Status
Please wait ...
Gene information
General information
TC003010 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4718593..4720710
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003010
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003010 RA
      MSLFRRVFL RKHALRTLF DNNFNHKQT RVLCITPNS 
      QEANSENAT QNRLKYLFL VAAGYLTYR LFSKEHLFP 
      TALAASNLK SRKKELNFV ADVVETSAP AVVYIEIKD 
      TRRVDFFTG RPTTISNGS GFIIREDGL ILTNAHVVA 
      NKPHSKVEV KLHNGATYN GYVEDFDMK SDLATVRIP 
      ARNLPTMKL GNSSELRPG EFVVAIGSP LALSNTVTF 
      GVISSTHRG SDELGLRGK DMVYLQTDA AITFGNSGG 
      PLVNLDGEA IGINSMKVT AGISFAIPS DYVKEFLKE 
      SLKKAGKVK HSRRYMGIT MLTLTPEIL NELQQRNQF 
      VPADVKSGV LVWKVILGS PADNGGLQP GDIVTHIDG 
      KVITGANDV YGVLSGTTN RTISMTVSR RGKKLEVYV 
      TPENLE
    CDS sequence
    • >TC003010 RA
      atgtctcta ttccgaaga gtttttctt cgtaaacac 
      gccttgcgc acccttttt gataataat ttcaaccac 
      aagcaaaca agagttttg tgtattaca ccaaatagt 
      caagaagca aactcagaa aatgcaacg caaaatcgc 
      ctaaaatat ctcttcctg gtggcagcg ggatactta 
      acctacaga ttatttagc aaagaacac ttgttcccc 
      accgctttg gccgcttct aatttgaaa agtcgcaaa 
      aaggagctt aatttcgtt gctgacgtg gttgaaact 
      tccgcacct gctgtggtc tacatcgaa attaaggac 
      accagaagg gttgatttc ttcacagga agacccacc 
      acgatatca aacgggtcc ggttttatt atcagggaa 
      gacggtctt atcttaaca aatgcgcat gttgttgcc 
      aataaaccg cattctaaa gtcgaggtg aaattacac 
      aatggggcg acttataac ggatacgtg gaagatttc 
      gatatgaaa agcgactta gccactgtt cgaatccct 
      gctagaaac ttgcccaca atgaaatta ggcaattcg 
      tctgaactg aggcctgga gaatttgtc gtagccata 
      ggcagcccc ctagctttg agtaacacg gtgactttt 
      ggggttatc agctcaaca cacaggggc tcagacgag 
      ttggggttg aggggcaag gacatggtg tatttgcag 
      actgatgcc gctattacg tttgggaat tcagggggg 
      cctttggtt aatttggac ggggaggcg attggtata 
      aacagtatg aaagtaacg gctgggatt tcattcgct 
      attcctagt gattacgta aaggaattt ttgaaagaa 
      agtcttaaa aaagcgggg aaagtcaaa cactcgagg 
      agatacatg ggcattaca atgctcact ttgacgccc 
      gaaattcta aatgagttg caacaaaga aatcagttt 
      gtgccagct gatgtcaag agtggagtt ctagtctgg 
      aaagtgata ttaggatca cctgctgac aatggaggt 
      ttgcaacct ggtgatatt gtcacacat attgatggg 
      aaagtaatt actggtgct aatgatgtg tatggtgtt 
      cttagtgga acaaccaat cggactata tctatgact 
      gttagtcgc cgtgggaaa aaactagaa gtatacgta 
      acgcccgaa aatttagaa taa
    mRNA sequence
    • >TC003010 RA
      attctttaa aaaacagta cttttacta tttttttta 
      ttacttttt attgatcag gacagttac gagtagtgt 
      cttgaataa aacatgaca tttaattcg cattttgac 
      agtttttta aataattgt cacgttttt tgtaaaaaa 
      attatgaaa acatacact tacatacaa taagcagct 
      aggaattcg gttatttaa ccaatttgg ggtaggaat 
      agaagattc agaagaata ttctgatta gacttgggc 
      caacaggta tgaagtgct tttatgagg ttacaaact 
      gtctgaaac tcctattat tacataacg aagaaatac 
      caaataagt cctgtgagg gccctgtgg tcaaaccat 
      gccttttac tccaatgac gcaactata atgttttaa 
      attggagtt caaatttta actgaaaat gtctctatt 
      ccgaagagt ttttcttcg taaacacgc cttgcgcac 
      cctttttga taataattt caaccacaa gcaaacaag 
      agttttgtg tattacacc aaatagtca agaagcaaa 
      ctcagaaaa tgcaacgca aaatcgcct aaaatatct 
      cttcctggt ggcagcggg atacttaac ctacagatt 
      atttagcaa agaacactt gttccccac cgctttggc 
      cgcttctaa tttgaaaag tcgcaaaaa ggagcttaa 
      tttcgttgc tgacgtggt tgaaacttc cgcacctgc 
      tgtggtcta catcgaaat taaggacac cagaagggt 
      tgatttctt cacaggaag acccaccac gatatcaaa 
      cgggtccgg ttttattat cagggaaga cggtcttat 
      cttaacaaa tgcgcatgt tgttgccaa taaaccgca 
      ttctaaagt cgaggtgaa attacacaa tggggcgac 
      ttataacgg atacgtgga agatttcga tatgaaaag 
      cgacttagc cactgttcg aatccctgc tagaaactt 
      gcccacaat gaaattagg caattcgtc tgaactgag 
      gcctggaga atttgtcgt agccatagg cagccccct 
      agctttgag taacacggt gacttttgg ggttatcag 
      ctcaacaca caggggctc agacgagtt ggggttgag 
      gggcaagga catggtgta tttgcagac tgatgccgc 
      tattacgtt tgggaattc aggggggcc tttggttaa 
      tttggacgg ggaggcgat tggtataaa cagtatgaa 
      agtaacggc tgggatttc attcgctat tcctagtga 
      ttacgtaaa ggaattttt gaaagaaag tcttaaaaa 
      agcggggaa agtcaaaca ctcgaggag atacatggg 
      cattacaat gctcacttt gacgcccga aattctaaa 
      tgagttgca acaaagaaa tcagtttgt gccagctga 
      tgtcaagag tggagttct agtctggaa agtgatatt 
      aggatcacc tgctgacaa tggaggttt gcaacctgg 
      tgatattgt cacacatat tgatgggaa agtaattac 
      tggtgctaa tgatgtgta tggtgttct tagtggaac 
      aaccaatcg gactatatc tatgactgt tagtcgccg 
      tgggaaaaa actagaagt atacgtaac gcccgaaaa 
      tttagaata atgtaactt gtactggaa taaattaag 
      atttacatt gcactctat ttgaatacc aaaaccatg 
      tttttgtta tcctagaag gcaatgact gtttgacag 
      tgaaattgt cacatcatc acagcctgc tagcattaa 
      ttcattcat tcactattt tttatttgc aatataaaa 
      actttatta ctttttaca tctttgttt tacgaatgg 
      taactgcat aacattcca ttattttat cgccattat 
      ttgcgtagg aaaatgaaa aataacaga cttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY