Status
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Gene information
General information
TC003006 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4786372..4795109
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003006
    iBeetle screen iB_00498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    potentially confused/crosscontaminated with: 0 remarks: animals lost,
    Pupal morphology
    potentially confused/crosscontaminated with: 0 remarks: animals lost,
    Adult morphology
    potentially confused/crosscontaminated with: 0 remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003006 RA
      MAQVPNADG FKNQLEAKA VTYHDIATK LLHDKFLLT 
      ALELHTELV ECGREVKQL KDFFSNPGN FEVPWQDTS 
      SRISRSESQ VTLDSLDLT RYSEDGAGT DERIAVLEF 
      ELRKAKETI NALRNNLTM ATESETSTP DKGCMRHLN 
      TDVIKPHEQ RALNFLINE YLLLNSYKL TSITFADEN 
      QHQDFDDWD DVGLNISKP PELITLYRE CFKQTSLDR 
      LSTGTQTDF DLSSYENLR QKIIEQEED LKKLEAKYK 
      NLESEYNEL KSIKETELL PSQNKSTPN SKDSLGSES 
      PSERFEIID KNMTSFKKR DSIITLEDN ISNSSLNVN 
      DWTNLTITT HDSVNNESA TKGIDSPKE KASKLINID 
      ISQEPFIKE VFSLCYVNI PQKVDNEIL DDILNYSVT 
      NENLVHTLS LSLLKIIPN IILNKREDA IPLLIGAVQ 
      LNSNSGERD KLLQQLFNL KKKPSDTER IMILTGLVG 
      IAKYSGENL VENEILPQC WEQLTDKHV EKRLLVAEA 
      CTALIPYVS SQIRNSLIL SMLQQLLED REDLVREAV 
      IRALSLVMV LCEDSDKYL QCEEITLNI LNDPSASIV 
      NLAIQILIP VLGKWALSI GRLKSHLIK YLLHKLSDY 
      SRDFESSKQ SNKILCIIE VLEALMPFV LISVVLHEQ 
      VVSNIEKDM TIDLRADLN KLCSNLTNP SVFCKWDHN 
      VGMVLYEFD KYIASNPTS SWDEMAWIL DMMIPDLLN 
      SLNHIDITY QSIIQNFIN LFSHICIFF GKSFTKFKI 
      QPIFQVQVN SLEQIFSNF NQYSPSLNI IPVYLVSVL 
      SYCDRDELS IVLKKFLCT LPLCGTPLD CLEVSVKGL 
      CENGLQTLV VSSLWEGVV HQSPLVRAV TADLFSAIV 
      GFCDKNLLS LKVTPGLVT LASDNDTLV KTAAIPALG 
      TLVTNCNEK EIHDKVYVQ FQNYLTDPS VKENHTVIR 
      QLIVTLGSI VNHCSAQFR QEVILPQLS LISTYLSQM 
      SNQTRKIDL ANALIEAFT TVVYSPFNK QDITSLIIP 
      GLRCLETAV TENQSLTCH RDIIGSMIR ECDDHAESS 
      THAQPEHSN KLQNVNQGV EEMKQRMSK IFTKPAGSK 
      PNTLPNLQS IFKKK
    CDS sequence
    • >TC003006 RA
      atggctcaa gtaccaaac gcagatggt ttcaaaaac 
      cagcttgaa gccaaagcc gtcacgtac cacgacatc 
      gccactaaa ttactgcat gacaaattc ctgctgact 
      gctttggaa ctgcacaca gaactggtc gaatgtgga 
      cgggaagtc aagcagttg aaggacttt ttctcaaat 
      ccggggaat tttgaagtt ccctggcag gacacttca 
      tcgcggatt tcgcgctct gaaagtcag gtcactttg 
      gatagtcta gatttgacc agatattca gaggatggg 
      gctgggact gatgaaagg attgcagtc cttgaattc 
      gagctgaga aaagcaaaa gaaaccatc aatgcgcta 
      agaaacaat ttaacaatg gcaacagaa tctgaaacc 
      agcactcct gataaggga tgcatgaga cacttgaac 
      accgacgta attaaacct cacgaacaa agagcctta 
      aatttttta atcaatgaa taccttctt ttaaactcg 
      tacaaacta acttccata actttcgct gatgaaaat 
      caacatcag gattttgat gattgggac gacgttggt 
      ttgaatatt tccaagcca cccgaactc atcactttg 
      tatagggag tgtttcaaa cagacgagc ttagaccgt 
      ttgtccact ggtacacaa accgatttc gacctcagc 
      tcttacgaa aatttgcga caaaaaatc attgaacag 
      gaggaagat ttgaaaaag ttggaggct aaatacaag 
      aatcttgaa tcggaatat aacgaactc aaaagtatt 
      aaagaaacc gagcttttg cctagtcaa aataaaagc 
      acacccaac agcaaagac tctctcgga agtgaatct 
      ccgtccgaa aggtttgaa attatcgac aaaaacatg 
      acaagtttt aagaaaagg gacagtatt ataacttta 
      gaagataac atttcaaac agcagtctc aacgtaaat 
      gactggacc aacttgacg attactaca catgacagc 
      gttaataac gaaagtgcc acaaaggga atcgactca 
      cctaaggaa aaagcttcc aagttaatt aatatcgat 
      attagtcaa gaaccgttt ataaaggaa gtgttctcg 
      ttatgctac gtcaatatt ccgcaaaaa gtggacaac 
      gagatacta gacgacatt ttaaactac tcagtaacc 
      aacgaaaat ttggttcac actttatcc ttgtctctc 
      ttgaaaatt attcccaac attatttta aacaaacgg 
      gaagatgcg attcccttg cttataggc gcagtccag 
      ttaaattca aacagtggt gaacgcgat aagttgttg 
      caacagttg tttaatctg aaaaagaag ccgtctgat 
      acggaacga ataatgatt ctcacaggg ttagtcggg 
      attgccaaa tattcggga gaaaattta gtggagaat 
      gagattttg ccgcagtgt tgggaacaa ctgactgat 
      aagcatgtg gaaaagcgg ctgcttgtg gcggaggct 
      tgcacagcc ctgattccc tacgtttca agccagatc 
      cgaaactcg ctcattctg tcaatgttg caacaactc 
      ctcgaagat cgcgaagac ctagtcagg gaggcagtc 
      atcagagct ttatcttta gtcatggtt ttatgtgaa 
      gactctgat aaatacctt cagtgtgaa gaaataacg 
      ctaaatatt ttaaacgac cctagtgcc agtatcgtc 
      aatctggcg attcaaatt ttaatcccg gttttagga 
      aagtgggct ttatccata gggcgcctc aagtcgcac 
      ttaattaag tatcttttg cacaaactt agcgactat 
      tctcgggat tttgagtcg agtaaacag tcgaataaa 
      atactgtgc attattgag gtcttggag gctttgatg 
      ccgtttgtg ttaatatcg gtggttttg cacgaacag 
      gttgtttca aatattgag aaagacatg acgattgat 
      ttaagagct gatttaaat aagttatgt tcgaattta 
      accaatccc tctgttttc tgcaagtgg gaccataac 
      gtgggcatg gtcctttac gagtttgat aaatatatc 
      gcctcaaac cccaccagt tcttgggac gagatggcg 
      tggattctg gacatgatg attccagat ttgttgaac 
      agcctgaac cacattgac ataacgtac cagtcaatt 
      atccaaaat ttcataaat ttattctcc cacatttgc 
      atctttttc ggcaaaagt ttcacaaaa tttaagata 
      cagcccatc ttccaagtg caagttaac agtcttgag 
      caaattttc tcaaacttc aatcagtat tccccaagt 
      ttgaacata attccagta tatctagtc tccgtttta 
      agctactgt gatcgagac gaactgtca attgttttg 
      aaaaagttt ttgtgtact cttccctta tgcggcact 
      ccgctcgat tgcttagaa gtttccgtc aaaggattg 
      tgtgaaaat ggtttacaa acacttgtt gtgtcaagt 
      ttatgggag ggggttgtc caccaaagt cctctagta 
      agagccgtc actgcggat ttgttttcg gcaattgtg 
      ggcttttgt gacaagaat cttctcagt ctgaaggta 
      acacccggt cttgttacg ttagccagt gataatgac 
      acattggtt aaaacggca gcaattcca gctctgggt 
      acacttgtg acaaactgt aatgagaaa gaaatacac 
      gacaaggtt tatgtgcaa tttcagaat tacttaacc 
      gatcccagt gtgaaggaa aatcacact gtgatcagg 
      caactgatc gtcactttg ggcagcatt gtaaatcac 
      tgctcggcg cagttcaga caagaagtg atcctgccg 
      cagctatcc ttaatctca acgtactta tcgcaaatg 
      tccaaccag acccgaaaa atcgactta gcaaacgcc 
      ttaatcgaa gctttcact acagtagtt tacagtccg 
      tttaacaag caagacatt acctcactc ataatccca 
      ggcttgcgc tgtttggag accgcagtg accgaaaac 
      cagtcacta acctgtcac cgagacatc atcggttcg 
      atgatccgc gaatgcgac gatcacgcc gaaagctcc 
      actcacgcc caaccagaa cactcgaat aaactccag 
      aacgtcaac caaggcgtt gaagagatg aagcaacgt 
      atgagcaaa attttcact aaaccagct ggatccaaa 
      ccaaatacc cttccaaac ctgcaaagc attttcaag 
      aagaagtga
    mRNA sequence
    • >TC003006 RA
      ataattaaa taatgcggt tacgtttgt tgttacatt 
      ttgaggaaa taaaaacaa tcacatgat cccaaggtc 
      ttggcgttt tgtgaattt gacaactgt ttttaaggt 
      tacaaaagt cagtcatgt caacgtgat gtatttgtt 
      gttgaagtg ttgtaacgg tgtaattgc acaagaaat 
      gcgtttcta gtttcgttg cattgtaaa catgcccag 
      tcctcactg aacactcat aatttgttg taacatgaa 
      agttacacc aaacactcc aacctagtt gtgaaccca 
      aaaatcccg gaaatggct caagtacca aacgcagat 
      ggtttcaaa aaccagctt gaagccaaa gccgtcacg 
      taccacgac atcgccact aaattactg catgacaaa 
      ttcctgctg actgctttg gaactgcac acagaactg 
      gtcgaatgt ggacgggaa gtcaagcag ttgaaggac 
      tttttctca aatccgggg aattttgaa gttccctgg 
      caggacact tcatcgcgg atttcgcgc tctgaaagt 
      caggtcact ttggatagt ctagatttg accagatat 
      tcagaggat ggggctggg actgatgaa aggattgca 
      gtccttgaa ttcgagctg agaaaagca aaagaaacc 
      atcaatgcg ctaagaaac aatttaaca atggcaaca 
      gaatctgaa accagcact cctgataag ggatgcatg 
      agacacttg aacaccgac gtaattaaa cctcacgaa 
      caaagagcc ttaaatttt ttaatcaat gaatacctt 
      cttttaaac tcgtacaaa ctaacttcc ataactttc 
      gctgatgaa aatcaacat caggatttt gatgattgg 
      gacgacgtt ggtttgaat atttccaag ccacccgaa 
      ctcatcact ttgtatagg gagtgtttc aaacagacg 
      agcttagac cgtttgtcc actggtaca caaaccgat 
      ttcgacctc agctcttac gaaaatttg cgacaaaaa 
      atcattgaa caggaggaa gatttgaaa aagttggag 
      gctaaatac aagaatctt gaatcggaa tataacgaa 
      ctcaaaagt attaaagaa accgagctt ttgcctagt 
      caaaataaa agcacaccc aacagcaaa gactctctc 
      ggaagtgaa tctccgtcc gaaaggttt gaaattatc 
      gacaaaaac atgacaagt tttaagaaa agggacagt 
      attataact ttagaagat aacatttca aacagcagt 
      ctcaacgta aatgactgg accaacttg acgattact 
      acacatgac agcgttaat aacgaaagt gccacaaag 
      ggaatcgac tcacctaag gaaaaagct tccaagtta 
      attaatatc gatattagt caagaaccg tttataaag 
      gaagtgttc tcgttatgc tacgtcaat attccgcaa 
      aaagtggac aacgagata ctagacgac attttaaac 
      tactcagta accaacgaa aatttggtt cacacttta 
      tccttgtct ctcttgaaa attattccc aacattatt 
      ttaaacaaa cgggaagat gcgattccc ttgcttata 
      ggcgcagtc cagttaaat tcaaacagt ggtgaacgc 
      gataagttg ttgcaacag ttgtttaat ctgaaaaag 
      aagccgtct gatacggaa cgaataatg attctcaca 
      gggttagtc gggattgcc aaatattcg ggagaaaat 
      ttagtggag aatgagatt ttgccgcag tgttgggaa 
      caactgact gataagcat gtggaaaag cggctgctt 
      gtggcggag gcttgcaca gccctgatt ccctacgtt 
      tcaagccag atccgaaac tcgctcatt ctgtcaatg 
      ttgcaacaa ctcctcgaa gatcgcgaa gacctagtc 
      agggaggca gtcatcaga gctttatct ttagtcatg 
      gttttatgt gaagactct gataaatac cttcagtgt 
      gaagaaata acgctaaat attttaaac gaccctagt 
      gccagtatc gtcaatctg gcgattcaa attttaatc 
      ccggtttta ggaaagtgg gctttatcc atagggcgc 
      ctcaagtcg cacttaatt aagtatctt ttgcacaaa 
      cttagcgac tattctcgg gattttgag tcgagtaaa 
      cagtcgaat aaaatactg tgcattatt gaggtcttg 
      gaggctttg atgccgttt gtgttaata tcggtggtt 
      ttgcacgaa caggttgtt tcaaatatt gagaaagac 
      atgacgatt gatttaaga gctgattta aataagtta 
      tgttcgaat ttaaccaat ccctctgtt ttctgcaag 
      tgggaccat aacgtgggc atggtcctt tacgagttt 
      gataaatat atcgcctca aaccccacc agttcttgg 
      gacgagatg gcgtggatt ctggacatg atgattcca 
      gatttgttg aacagcctg aaccacatt gacataacg 
      taccagtca attatccaa aatttcata aatttattc 
      tcccacatt tgcatcttt ttcggcaaa agtttcaca 
      aaatttaag atacagccc atcttccaa gtgcaagtt 
      aacagtctt gagcaaatt ttctcaaac ttcaatcag 
      tattcccca agtttgaac ataattcca gtatatcta 
      gtctccgtt ttaagctac tgtgatcga gacgaactg 
      tcaattgtt ttgaaaaag tttttgtgt actcttccc 
      ttatgcggc actccgctc gattgctta gaagtttcc 
      gtcaaagga ttgtgtgaa aatggttta caaacactt 
      gttgtgtca agtttatgg gagggggtt gtccaccaa 
      agtcctcta gtaagagcc gtcactgcg gatttgttt 
      tcggcaatt gtgggcttt tgtgacaag aatcttctc 
      agtctgaag gtaacaccc ggtcttgtt acgttagcc 
      agtgataat gacacattg gttaaaacg gcagcaatt 
      ccagctctg ggtacactt gtgacaaac tgtaatgag 
      aaagaaata cacgacaag gtttatgtg caatttcag 
      aattactta accgatccc agtgtgaag gaaaatcac 
      actgtgatc aggcaactg atcgtcact ttgggcagc 
      attgtaaat cactgctcg gcgcagttc agacaagaa 
      gtgatcctg ccgcagcta tccttaatc tcaacgtac 
      ttatcgcaa atgtccaac cagacccga aaaatcgac 
      ttagcaaac gccttaatc gaagctttc actacagta 
      gtttacagt ccgtttaac aagcaagac attacctca 
      ctcataatc ccaggcttg cgctgtttg gagaccgca 
      gtgaccgaa aaccagtca ctaacctgt caccgagac 
      atcatcggt tcgatgatc cgcgaatgc gacgatcac 
      gccgaaagc tccactcac gcccaacca gaacactcg 
      aataaactc cagaacgtc aaccaaggc gttgaagag 
      atgaagcaa cgtatgagc aaaattttc actaaacca 
      gctggatcc aaaccaaat acccttcca aacctgcaa 
      agcattttc aagaagaag tgatagtgc cctgaagga 
      ggtagctct ctttcgcaa tcgctaaat attctgtga 
      ttaacggga atagagtag agtagaggt agagattta 
      agagagatc ttattgttt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY