| General information | |||
| TC002995 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5131925..5142969 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002995 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00496 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
- >TC002995 RA
MFLRFAVLC LVLCLQNVA SEWKKPTVL IAVLARNKA HTLPYFLTT LENLDYPKN RISLWIRSD HNSDKTIEI LRKWINAVK DEYRMISTE FVEENEGYP DESGPAHWT PERFNHVID LRESSLNFA RKIWADYYW TIDCDVFLT NPKTLDILI SKGYTVVAP MLKSDGLYS NFWYGMTDD YYYQRTEDY KPVVNRENI GCFNVPMVH SCVLVDLRR QVSDFLTYD PDKVPNFDG PIDDIITFA IGANRSGIS LNLCNDERF GFVMLPLEQ DANLAIDFE QLTNIKLEV LTEEKPLNV SKLLEAYTW LPEKDTLGF DKIFMINLL RRPERRQRM RFCFDELGL QVTIVDAVD GRALNASIL QQISPLPEY ADPYHKRPM TLGEIGCFL SHYNIWKDI VRNGYETTL VLEDDIRFE SFFRTKVLN VMDEVKRVS GWDLVYLGR KRLQEDDEP WVEGSSLLV EAGYSYWTL GYVLSLNGA KKLLNADPL SRLVPVDEY LPILFDKHP QTSWKGYFP KRNLVALSA APLLVYPTH YTGEQGYIS DTEDSVIIK ENGPRARDD L
- >TC002995 RA
atgtttttg cgcttcgcg gtgctgtgt cttgttttg tgtttacaa aacgtggct agtgaatgg aaaaaaccc acggttctc atcgctgtt ttggcccga aacaaagct catacgtta ccttatttt ctcacaact ttggaaaat ctcgattat cccaaaaat cggatttct ttgtggata agaagcgac cacaatagt gataaaacg attgaaatt ttgcggaaa tggatcaat gcggtcaag gatgaatat cgtatgatt agcaccgaa tttgtggaa gagaatgaa gggtatcct gacgaaagt ggacctgct cattggacc ccggaacgg tttaaccat gttatagac ttgagagag tcatctttg aatttcgcc agaaaaatc tgggctgat tattattgg acaatcgat tgcgacgtt tttctaaca aaccctaaa acacttgac atactaata tcaaagggc tacactgta gtggccccc atgctgaaa tccgacgga ttgtattca aatttctgg tacggaatg accgacgat tactattac caaagaaca gaagactat aagcccgta gtaaaccgc gaaaatatt ggttgtttt aacgtgcca atggtccac tcttgcgtc ctcgttgat ttgaggaga caagtctca gattttcta acttatgat ccggataaa gtgccgaat tttgatggc cctattgat gatattatc acgtttgct atcggagca aacaggagc ggaatctca ttaaattta tgcaacgat gaacggttt ggatttgtt atgttgccc ttggaacaa gacgctaac ttagcgatt gattttgaa caactgacg aatattaag ttggaggtt ctgactgaa gagaaacct ctaaacgtt agcaaattg ttggaggcc tatacgtgg cttcctgaa aaagatact ttagggttt gataaaatt tttatgatc aatttgctg cgacgaccg gaaaggagg caacggatg aggttctgc tttgacgag ctgggactt caagtcaca atcgtcgac gcggtggat ggaagggcg ctgaatgcg agtattttg cagcagata tctcctttg cccgaatat gcggaccct tatcacaaa agaccgatg acactcgga gaaattggg tgcttttta agtcattat aacatctgg aaggatatt gttcggaac ggatatgag accactctt gttttagag gatgatatt agatttgaa tcctttttc cgaactaaa gtgttgaat gtgatggac gaggtaaag cgtgtttcc ggctgggat ttggtgtat ttaggccgt aaaaggttg caggaagac gatgaaccg tgggtggag gggtcaagt ttgttagtc gaggcgggt tattcctac tggacttta ggatatgtc cttagcctg aacggagcc aaaaaactt ttaaacgct gatcctctc tccagactt gtgccagtt gacgagtat ttaccgata ttatttgat aagcatcca cagacgagt tggaagggt tatttcccc aagagaaat ttggttgca ttatcggcc gctccctta ctggtctat cccacgcac tatacggga gaacagggc tatattagc gacacggag gactctgtt attatcaag gaaaatgga ccacgagct cgcgacgat ctttga
- >TC002995 RA
gctaaaatt tgaaaagtg tggttcgaa atttattta taaaatagt tttacaata cgtttccaa tgattttgc gctaaagtt taaaaatgt ttttgcgct tcgcggtgc tgtgtcttg ttttgtgtt tacaaaacg tggctagtg aatggaaaa aacccacgg ttctcatcg ctgttttgg cccgaaaca aagctcata cgttacctt attttctca caactttgg aaaatctcg attatccca aaaatcgga tttctttgt ggataagaa gcgaccaca atagtgata aaacgattg aaattttgc ggaaatgga tcaatgcgg tcaaggatg aatatcgta tgattagca ccgaatttg tggaagaga atgaagggt atcctgacg aaagtggac ctgctcatt ggaccccgg aacggttta accatgtta tagacttga gagagtcat ctttgaatt tcgccagaa aaatctggg ctgattatt attggacaa tcgattgcg acgtttttc taacaaacc ctaaaacac ttgacatac taatatcaa agggctaca ctgtagtgg cccccatgc tgaaatccg acggattgt attcaaatt tctggtacg gaatgaccg acgattact attaccaaa gaacagaag actataagc ccgtagtaa accgcgaaa atattggtt gttttaacg tgccaatgg tccactctt gcgtcctcg ttgatttga ggagacaag tctcagatt ttctaactt atgatccgg ataaagtgc cgaattttg atggcccta ttgatgata ttatcacgt ttgctatcg gagcaaaca ggagcggaa tctcattaa atttatgca acgatgaac ggtttggat ttgttatgt tgcccttgg aacaagacg ctaacttag cgattgatt ttgaacaac tgacgaata ttaagttgg aggttctga ctgaagaga aacctctaa acgttagca aattgttgg aggcctata cgtggcttc ctgaaaaag atactttag ggtttgata aaattttta tgatcaatt tgctgcgac gaccggaaa ggaggcaac ggatgaggt tctgctttg acgagctgg gacttcaag tcacaatcg tcgacgcgg tggatggaa gggcgctga atgcgagta ttttgcagc agatatctc ctttgcccg aatatgcgg acccttatc acaaaagac cgatgacac tcggagaaa ttgggtgct ttttaagtc attataaca tctggaagg atattgttc ggaacggat atgagacca ctcttgttt tagaggatg atattagat ttgaatcct ttttccgaa ctaaagtgt tgaatgtga tggacgagg taaagcgtg tttccggct gggatttgg tgtatttag gccgtaaaa ggttgcagg aagacgatg aaccgtggg tggaggggt caagtttgt tagtcgagg cgggttatt cctactgga ctttaggat atgtcctta gcctgaacg gagccaaaa aacttttaa acgctgatc ctctctcca gacttgtgc cagttgacg agtatttac cgatattat ttgataagc atccacaga cgagttgga agggttatt tccccaaga gaaatttgg ttgcattat cggccgctc ccttactgg tctatccca cgcactata cgggagaac agggctata ttagcgaca cggaggact ctgttatta tcaaggaaa atggaccac gagctcgcg acgatcttt gatgcagat ttatttacg tgaatgttc ctactaact tgacctcac atttttcat taggtggcg acagtgcaa tttcttatt tatgtgcca tttgttaat gccatttat tattacgat aagctgcaa gttgcttat aaaagtgcc ttataacaa tattttatg tcaaagaat tttataaca caataaaat aataatacc aag
