Status
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Gene information
General information
TC002993 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5158413..5161725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002993
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 88.89% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002993 RA
      MTNTNVDDL TSALQGTRV SPTGVTFAG QSLKLDTVD 
      DAKSVIEEI EKCTNLQFL NLEGNTLGV EASKGIAKA 
      LEKHPEFKR ALWKDMFTG RMKTEIPKA LEFLGSGLV 
      TAGARLTEL DMSDNAFGP IGVEGLAAL LRSSSCYAL 
      EELRLNNNG LGITGGKLL ASALTDCYN SSKETGKPL 
      QLKVFIAGR NRLENEGAK ALAEVFKMI GTLEEIAMP 
      QNGIYHVGI SALSEAFTQ NKNLQILNL NDNTIGEKG 
      AEAIAKALP HLQQLKEIN FGDCLLKTA GAISLANVL 
      KTGHANLEE LTLGHNEIR LKGGLQLVK AMANKSKLK 
      VLNLNGNQF GEDGRKQIE TEIKQIGKL NSLGSLSGD 
      ESEDSEEET SEEVSHTES DMEEEIIEP KTATDFLNS 
      PTEEIFIAL GDARNELIL REAKKGDDI DVNTFIPVL 
      MKVASLANS SKENVSKLA LSCSERLYK ELFTWSDKT 
      NNSCLVNNT ILIHLGLIK AEDKKFKPT WNLDACAKA 
      LRSVMDKNY VTEITKNTF TVFLQKDKS GSR
    CDS sequence
    • >TC002993 RA
      atgacaaat accaatgta gacgacctt acaagtgcc 
      ttacaaggc actcgtgtg agccccact ggggtcacc 
      tttgccggc cagtcttta aaacttgat actgttgat 
      gacgcaaaa agcgtaatt gaagagata gaaaaatgc 
      accaacctc caattttta aacttggaa ggcaatact 
      ttgggagtt gaagcgtcc aaaggcatt gccaaagca 
      ttggaaaaa catcccgag tttaagaga gcgctatgg 
      aaggacatg tttacgggc agaatgaaa acagaaata 
      ccgaaagct ttggaattt cttggtagt ggtttagta 
      acggcaggt gcccgcttg actgaactg gacatgagt 
      gacaatgct ttcggccca attggtgtc gaaggactt 
      gctgctctt ttgagaagc tcctcatgc tatgcttta 
      gaagaactc aggttaaat aataacggc ctaggcatc 
      actggtggt aaattgtta gcgtcagcc ctaacagac 
      tgctataac tctagtaaa gaaacggga aaaccactc 
      cagctcaag gtttttatc gctggaaga aaccgactt 
      gagaatgaa ggtgcaaaa gcacttgct gaggttttc 
      aagatgatt ggtacactt gaagaaata gcaatgccc 
      cagaatggt atttaccac gttggcata tcggcacta 
      tctgaggct tttacccaa aataaaaat ctgcaaatt 
      ttaaacttg aatgacaat acgattggt gaaaagggg 
      gctgaggcc attgctaaa gccctccca cacttgcaa 
      cagttgaaa gaaatcaat tttggcgac tgtttgctg 
      aaaactgcc ggagctatt tctctagca aatgtttta 
      aaaactggt catgccaac ttggaggag ttaacactt 
      gggcacaac gaaattcgc ctgaaaggg gggcttcag 
      ttggttaaa gccatggcg aataaaagt aaattgaaa 
      gtgttaaat ttgaacgga aatcagttt ggggaagac 
      ggtcgtaaa caaattgaa accgaaatt aaacaaata 
      ggcaaatta aattccttg ggaagttta agcggggat 
      gagtctgag gacagtgag gaggaaacc agtgaagaa 
      gtgtcgcac actgagagt gacatggag gaggaaatc 
      attgagcca aaaacagcc accgatttt ctgaattcg 
      ccaactgag gaaattttt attgcactt ggtgatgcc 
      agaaatgag ctgatttta agagaagct aaaaaaggt 
      gacgatatt gatgtcaac acttttatt cccgtttta 
      atgaaagtc gcgtctctt gccaacagc agcaaagaa 
      aacgtctct aaattggcg ctttcttgc tcggaaagg 
      ttgtacaaa gaactgttc acatggtct gacaaaacg 
      aacaattct tgtctggtg aacaacaca attttgata 
      catttaggc ttaataaag gctgaagat aaaaaattt 
      aagccaacg tggaacttg gacgcttgt gccaaggcg 
      ctgaggagc gttatggac aaaaattac gtcacggaa 
      attaccaaa aataccttc actgtgttt ttacaaaaa 
      gataaaagt ggaagccga tga
    mRNA sequence
    • >TC002993 RA
      tgaatatct gaaccaact ttaaaaaat tccaacggc 
      ttgacacgc gtgagcgat tgtaattta ttaaataat 
      agttgtagt attgtgtaa ataataaaa atgacaaat 
      accaatgta gacgacctt acaagtgcc ttacaaggc 
      actcgtgtg agccccact ggggtcacc tttgccggc 
      cagtcttta aaacttgat actgttgat gacgcaaaa 
      agcgtaatt gaagagata gaaaaatgc accaacctc 
      caattttta aacttggaa ggcaatact ttgggagtt 
      gaagcgtcc aaaggcatt gccaaagca ttggaaaaa 
      catcccgag tttaagaga gcgctatgg aaggacatg 
      tttacgggc agaatgaaa acagaaata ccgaaagct 
      ttggaattt cttggtagt ggtttagta acggcaggt 
      gcccgcttg actgaactg gacatgagt gacaatgct 
      ttcggccca attggtgtc gaaggactt gctgctctt 
      ttgagaagc tcctcatgc tatgcttta gaagaactc 
      aggttaaat aataacggc ctaggcatc actggtggt 
      aaattgtta gcgtcagcc ctaacagac tgctataac 
      tctagtaaa gaaacggga aaaccactc cagctcaag 
      gtttttatc gctggaaga aaccgactt gagaatgaa 
      ggtgcaaaa gcacttgct gaggttttc aagatgatt 
      ggtacactt gaagaaata gcaatgccc cagaatggt 
      atttaccac gttggcata tcggcacta tctgaggct 
      tttacccaa aataaaaat ctgcaaatt ttaaacttg 
      aatgacaat acgattggt gaaaagggg gctgaggcc 
      attgctaaa gccctccca cacttgcaa cagttgaaa 
      gaaatcaat tttggcgac tgtttgctg aaaactgcc 
      ggagctatt tctctagca aatgtttta aaaactggt 
      catgccaac ttggaggag ttaacactt gggcacaac 
      gaaattcgc ctgaaaggg gggcttcag ttggttaaa 
      gccatggcg aataaaagt aaattgaaa gtgttaaat 
      ttgaacgga aatcagttt ggggaagac ggtcgtaaa 
      caaattgaa accgaaatt aaacaaata ggcaaatta 
      aattccttg ggaagttta agcggggat gagtctgag 
      gacagtgag gaggaaacc agtgaagaa gtgtcgcac 
      actgagagt gacatggag gaggaaatc attgagcca 
      aaaacagcc accgatttt ctgaattcg ccaactgag 
      gaaattttt attgcactt ggtgatgcc agaaatgag 
      ctgatttta agagaagct aaaaaaggt gacgatatt 
      gatgtcaac acttttatt cccgtttta atgaaagtc 
      gcgtctctt gccaacagc agcaaagaa aacgtctct 
      aaattggcg ctttcttgc tcggaaagg ttgtacaaa 
      gaactgttc acatggtct gacaaaacg aacaattct 
      tgtctggtg aacaacaca attttgata catttaggc 
      ttaataaag gctgaagat aaaaaattt aagccaacg 
      tggaacttg gacgcttgt gccaaggcg ctgaggagc 
      gttatggac aaaaattac gtcacggaa attaccaaa 
      aataccttc actgtgttt ttacaaaaa gataaaagt 
      ggaagccga tgatcgtgc tttaatatt tttaactga 
      attaaaaac tgccaaaaa tggatgtgt atctcatag 
      tatagacaa caaataaac acatttccc aaattacaa 
      aatttattt aatcattca aaacaatca gttctctaa 
      aacaagact aaattattt tccactatg tgtaaactt 
      tcattgagg agcggatgc gtttcctgc agatggggc 
      actgcccac tgtagcgca tcagtctgg gggtacaat 
      cgtcacaca aacataagt gtcgacaag gcaaaacta 
      acacacatt tagagttat cctgacaga ctacacaat 
      aaccctcgt ttttttcgt cttcattat ttgtactca 
      gtggtttac attctatta cttgtctag aactatgtt 
      ttaaagcaa tacgcgaca agaaacgta aagttgcca 
      ctttcacat gattgtact tcagtctta aagcggtaa 
      aaaacgaag acactatgt ttctgaaat aggagaata 
      ggacagaat agaactggg aaaaaatgt acaagaaac 
      ataacccga ctatcaaac ctatgagcg attctagcg 
      gtacgtctg ttacaaagt ggtaacaac tgtaaattg 
      agtttaaaa ttaacttat aaactagcg taacattgt 
      tacaaatgc tgtctaaga tgtaaacac acaaacttt 
      tcaagtaat tgtacgtca gaggcccca gagacagta 
      cagattttg gtacaaaga ctatgataa accacactc 
      cgtcaccaa acagtataa caacttgct tcataatga 
      caaaaatgt gtttaactg cgctcacta ttgcgagta 
      ttccattta taggagcag tgcaaactc cagaaagcc 
      attttaaga caccagtta gttgttggc aatagttga 
      gtttttcag ccagccatt caaaaacgt agaccacac 
      tttctgtta gacaatgaa gacggtgga tcacatctt 
      ggagaaata tggtaaagt cttccaaaa ttaccgtca 
      acagacaaa atagcttgt ttatgaacg atccggccg 
      tcgtcagaa gaaaagcca aacttgtga caataaatt 
      gccccgttt tcacactga tgtaaaacg tgtacgtaa 
      ttgccgcct ttaaagtgg tgataacgg ccaacattg 
      tgcaaaaac ttttctcat cgtaacttt tccaaaaac 
      ttgaaagtt cttggtttg taggttaga aatttgaat 
      ttgatcaag ggttttgat tttgacaaa accaaagtc 
      actaacgtg ggagcgtcg tgtacaagg gacgaataa 
      aaagtttac taccgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY