Status
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Gene information
General information
TC002991 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5187077..5191065
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002991
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00493

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002991 RA
      MDHVALDVS GTSDSIHDR KRTKSLRRL WNQLSKCQR 
      SCIYTIVIV LTLTVFYLV STESERTQI SLAESANEN 
      KKEVLHNIR NDPRDVDTV FHDNDSEGQ VLEEPGGLE 
      ADSNRNQVL LPPKVVKKF AGPTTARQE AVVKAFKHA 
      WQGYKQFAW GHDHLKPIT EGYSDWFGL GLSIVDSID 
      TIYIMGLTK EYKEAREWI EKHLHFDVN RDVNLFEVT 
      IRVLGGLLS IYHLTQDRM FLTKAVDLA DRLLPSFES 
      ESGIPYSDV NLFTRKAHA PKWSPDSST SEVTTIQLE 
      FRDLSRITG NPKYENAVS KVSLHIHNL EKKDGLVPI 
      FINANTGQF RSYATITLG ARGDSYYEY LLKQWLQTG 
      KTIDYLRDD YVTSIMGVE KHLTKRTVP NGFLFIGEL 
      LVGGKDFKP KMDHLTCYL PGTLALGVH NGLPESHMK 
      LAEELLTTC YQTYAQQPT FLAPEITYF NIQGENSND 
      MYVKTNDAH NLLRPEYLE SLWYVYQLT GNKTYQDWG 
      WQIFQGFEN YTKVKNGYT SIGNVKNPL NVRPKDMME 
      SFFLSETLK YLYLLFSDD PKLIDLDTY VINSEAHPL 
      SIYSS
    CDS sequence
    • >TC002991 RA
      atggaccat gtcgccctt gatgtttcc ggtaccagc 
      gacagcatc cacgaccgc aaaagaacc aaaagctta 
      agaaggctc tggaaccag ttatcaaaa tgtcaaagg 
      agctgtatc tacaccata gtcatagtg ttaaccctc 
      actgtcttt tacctagtc tcgaccgaa tccgagcga 
      acgcagata agcctagcg gagagcgcg aatgagaac 
      aagaaggag gtgctccac aacatcagg aacgacccc 
      cgagacgtc gacaccgtg ttccatgat aacgacagc 
      gaggggcaa gtgctggag gagcccggc ggcttggag 
      gcggacagt aacaggaac caggtgctg ttgccgccc 
      aaagtcgtg aagaagttc gcgggcccc acaacggcg 
      cgccaggaa gccgtggtg aaggcgttc aagcacgcc 
      tggcaagga tacaagcaa tttgcgtgg ggtcatgac 
      cacttaaaa cccatcacc gaggggtac agtgactgg 
      tttgggctg ggtttgagc attgttgac tcgattgac 
      accatttac ataatggga ttaacaaaa gaatacaaa 
      gaagcgcgt gagtggatt gaaaaacac ctacatttt 
      gatgtgaac cgagacgtt aacttgttt gaagtaaca 
      atacgagtt ttgggaggc ctacttagc atatatcac 
      cttactcag gacaggatg tttctcact aaagctgtt 
      gatttagct gatcgatta ctgccaagt tttgagtcg 
      gagtcgggg attccctat tctgatgtt aatttattc 
      acacggaaa gctcatgca cccaagtgg tcacccgat 
      agcagcacg tctgaagtg actaccatt caactagaa 
      tttcgggat ttaagcaga atcactgga aatcccaaa 
      tacgaaaat gctgtttca aaagtgtca cttcatatc 
      cataatttg gaaaaaaaa gatggtttg gttcctatt 
      tttatcaat gccaacact ggacaattt aggtcttat 
      gctactata actctagga gctcgcggt gacagctat 
      tacgagtat ttactgaaa cagtggttg caaacagga 
      aaaacgatc gattattta agggacgat tacgtgacg 
      agtataatg ggtgttgag aaacattta acgaaaaga 
      accgtccct aacggtttc ctctttatt ggcgaattg 
      ctagtcggt ggtaaagat tttaaaccg aaaatggac 
      cacctgacg tgctatttg cctggaact ttggccctt 
      ggggtacac aatggtctt ccagaaagt cacatgaaa 
      ctagcagaa gaactgctt accacttgt tatcagaca 
      tacgcacaa caacccaca tttctagca cccgaaatc 
      acgtatttt aatatccag ggcgaaaac tccaacgac 
      atgtacgta aaaaccaac gacgcgcat aatttgctg 
      cggcccgag tatttggag agtctctgg tacgtgtac 
      cagctcact ggtaacaag acgtaccag gactggggc 
      tggcagata tttcaaggg tttgagaat tacacaaaa 
      gtgaaaaac ggctacact tcgattgga aacgtgaaa 
      aacccgctg aatgtgaga ccaaaggat atgatggaa 
      tcattcttt cttagtgaa accttaaag tatttatat 
      ttgttattt agtgacgat ccgaaactg atagacttg 
      gatacctat gttattaat tcggaagcg caccctttg 
      tctatatac tcgtcgtga
    mRNA sequence
    • >TC002991 RA
      actgaaaaa tttctctat aaattgttg gaaaatgcc 
      ctcaaagtg tttagaaga acgaaagtt tgtcaaacc 
      gcgtctgcg cgcccagtc caatttttg acaattttg 
      acataactt tcaatttct gtttgtgat gtgtacgtg 
      aattagaat aagttttat cgtagtttt atcatcttg 
      tacagaaag tggcccctc tttaacaat gctagtgtg 
      ttaaaaccg ccgtaacga tggaccatg tcgcccttg 
      atgtttccg gtaccagcg acagcatcc acgaccgca 
      aaagaacca aaagcttaa gaaggctct ggaaccagt 
      tatcaaaat gtcaaagga gctgtatct acaccatag 
      tcatagtgt taaccctca ctgtctttt acctagtct 
      cgaccgaat ccgagcgaa cgcagataa gcctagcgg 
      agagcgcga atgagaaca agaaggagg tgctccaca 
      acatcagga acgaccccc gagacgtcg acaccgtgt 
      tccatgata acgacagcg aggggcaag tgctggagg 
      agcccggcg gcttggagg cggacagta acaggaacc 
      aggtgctgt tgccgccca aagtcgtga agaagttcg 
      cgggcccca caacggcgc gccaggaag ccgtggtga 
      aggcgttca agcacgcct ggcaaggat acaagcaat 
      ttgcgtggg gtcatgacc acttaaaac ccatcaccg 
      aggggtaca gtgactggt ttgggctgg gtttgagca 
      ttgttgact cgattgaca ccatttaca taatgggat 
      taacaaaag aatacaaag aagcgcgtg agtggattg 
      aaaaacacc tacattttg atgtgaacc gagacgtta 
      acttgtttg aagtaacaa tacgagttt tgggaggcc 
      tacttagca tatatcacc ttactcagg acaggatgt 
      ttctcacta aagctgttg atttagctg atcgattac 
      tgccaagtt ttgagtcgg agtcgggga ttccctatt 
      ctgatgtta atttattca cacggaaag ctcatgcac 
      ccaagtggt cacccgata gcagcacgt ctgaagtga 
      ctaccattc aactagaat ttcgggatt taagcagaa 
      tcactggaa atcccaaat acgaaaatg ctgtttcaa 
      aagtgtcac ttcatatcc ataatttgg aaaaaaaag 
      atggtttgg ttcctattt ttatcaatg ccaacactg 
      gacaattta ggtcttatg ctactataa ctctaggag 
      ctcgcggtg acagctatt acgagtatt tactgaaac 
      agtggttgc aaacaggaa aaacgatcg attatttaa 
      gggacgatt acgtgacga gtataatgg gtgttgaga 
      aacatttaa cgaaaagaa ccgtcccta acggtttcc 
      tctttattg gcgaattgc tagtcggtg gtaaagatt 
      ttaaaccga aaatggacc acctgacgt gctatttgc 
      ctggaactt tggcccttg gggtacaca atggtcttc 
      cagaaagtc acatgaaac tagcagaag aactgctta 
      ccacttgtt atcagacat acgcacaac aacccacat 
      ttctagcac ccgaaatca cgtatttta atatccagg 
      gcgaaaact ccaacgaca tgtacgtaa aaaccaacg 
      acgcgcata atttgctgc ggcccgagt atttggaga 
      gtctctggt acgtgtacc agctcactg gtaacaaga 
      cgtaccagg actggggct ggcagatat ttcaagggt 
      ttgagaatt acacaaaag tgaaaaacg gctacactt 
      cgattggaa acgtgaaaa acccgctga atgtgagac 
      caaaggata tgatggaat cattctttc ttagtgaaa 
      ccttaaagt atttatatt tgttattta gtgacgatc 
      cgaaactga tagacttgg atacctatg ttattaatt 
      cggaagcgc accctttgt ctatatact cgtcgtgat 
      ttttttata ggtagatgc aatacgtct agtcgattt 
      tacatgagg gggatttat taatttgtt agtgagatt 
      ttaatttcc gtataaaac ttggaaagc aaggtcctc 
      tgtttttgt ctgttttgt cacctaagt gggccagta 
      tttaccagc aggatgtac ttacgatta agtaaaaaa 
      tgtcaagta tttagaaat gtgtcattt tccattcca 
      agactgagt gagattggg tttgatatt aattacaaa 
      aactgactt cctaaacct aagtgttga attttattt 
      ttatactca cgtttttgt ttaaaccgc catgacttg 
      acaaatatt accgttttg atgtattct tttcgcagt 
      ataattgaa taagctcgt tgataggaa atgtaatat 
      cgatagtta attgttgtg ttatttagt ttattaggc 
      caagaatgt taatttcac tgttggata tgatggtat 
      cgatggtag aactgcacg cttctgagt gtatgtttg 
      tttattgga cgtgctgat tctttcggt ttttacaca 
      cgaaacatt caactcagt agcctgtct agaaggaat 
      tacgttaat ttataactc cgatttgtg ggtaaatta 
      tttcacttt gtaggaacg atcaataaa tttttactc 
      acatttcaa tgtctctac cctagagca agaaaacca 
      ttcgcacta tcgcacaaa gcaatttat tgcaccttt 
      ttgtttcac tattaacaa atgcaggaa atatttttt 
      acaaataaa tacacaaat gtatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY