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Gene information
General information
TC032375
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002989
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5223244..5225435
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032375
    iBeetle screen iB_00492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032375 RA
      MSFSVPFLL LSCALTQVL GQYEPTWES LDSRPLPDW 
      YDKAKVGIF LHWGVYSVP SFGSEWFWK FWQDEEEPY 
      VNFMQDNYP PDFTYQDFA KDFTAEFYD PEQWADLFA 
      KSGAKYVIL TSKHHEGYA MWPSKYSYS WNANDVGPN 
      RDLLGELAD AIRNSTDLH FGLYHSLFE WFHPLYDSD 
      RDNNFTTSE FVDYKIIPE MKELVETYE PEVLWSDGD 
      GEALDTYWK SLEFLAWLY NESPVKDFV VTNDRWGSN 
      VGCKHGGFY TCADRYNPG HLLPHKWEN CMTIDKKSW 
      GYRRNADLD EFLTTHELI TTLAETISC GGNILINVG 
      PTKAGTIIP LFQERLLDL GTWLSINGD AIYESSAWS 
      VQNDSFTSG VWYTAKDPV VYAIVLNWP IDNVLYLGS 
      AKSLFDNGK TTQVTMLGN ENGDLEAEV VDTTNLVAV 
      TFPDRASVE SEWAWVLKI TPGS
    CDS sequence
    • >TC032375 RA
      atgtctttc tccgtgccc ttcctgttg ctttcctgt 
      gcactaacc caagttttg ggccagtat gagccaact 
      tgggagagc ctcgacagc cgccctttg cccgactgg 
      tacgacaaa gccaaagtt gggattttc ctccactgg 
      ggcgtttac tcagtgccc agtttcggg agcgaatgg 
      ttctggaaa ttctggcaa gatgaagaa gaaccgtat 
      gtgaacttt atgcaagac aactatccg cccgacttc 
      acttatcaa gattttgca aaagatttc accgccgaa 
      ttctacgat cctgagcag tgggcggac ttgtttgcc 
      aaatctggg gccaaatat gttatcctg accagtaaa 
      catcacgaa ggttacgcc atgtggcct tcgaaatat 
      tcgtacagt tggaatgcc aacgacgtg gggcccaat 
      agagaccta ctaggagaa cttgccgat gcaattagg 
      aacagtaca gacctacac ttcggtctg taccactct 
      ctgttcgag tggtttcac cccctgtat gacagcgat 
      cgggataat aatttcacg acttctgaa tttgtcgac 
      tataaaatt attccagaa atgaaagaa ttggtcgaa 
      acttacgaa ccggaagtt ttatggtcg gatggagat 
      ggagaggct ttggatacg tactggaag tcactggag 
      tttctggct tggctttac aatgaaagc cctgtcaag 
      gattttgtc gtaactaac gacagatgg ggaagtaac 
      gttgggtgc aaacatggg ggtttctac acctgtgct 
      gatagatac aaccctgga catttgttg ccccacaag 
      tgggaaaac tgcatgaca attgacaaa aaatcttgg 
      ggttataga agaaatgcc gatctcgac gaattccta 
      acgacacat gaactgatt accacactt gccgaaacc 
      attagttgt ggagggaat attttaata aacgttgga 
      ccgacaaaa gccggaaca attatccca cttttccag 
      gaacgttta ctagatctt ggaacttgg ttgtccatt 
      aacggtgat gcaatttac gaaagtagc gcttggagt 
      gtacaaaac gactctttc acttctgga gtctggtac 
      acagctaaa gatccagtg gtgtatgca atagtttta 
      aactggccg attgataat gttctgtac ttgggctcg 
      gcaaagtct ctctttgac aatggtaaa accactcaa 
      gttacgatg cttgggaat gaaaatggg gatcttgag 
      gctgaagtt gttgatacg actaatctg gtagctgtg 
      acttttccg gatagagca agtgttgaa agtgagtgg 
      gcttgggtg ctcaaaatc acgcctggt tcttaa
    mRNA sequence
    • >TC032375 RA
      gtcagtatt aaaagtgct ttagtatcg gtattgagc 
      aacccatgg acaaacctg ttaagactc tgctaaaaa 
      gctctgact gatatgaac tcgtaataa ccgaacaaa 
      gcaagctga ttacaggac gccgagcag tcatgtctt 
      tctccgtgc ccttcctgt tgctttcct gtgcactaa 
      cccaagttt tgggccagt atgagccaa cttgggaga 
      gcctcgaca gccgccctt tgcccgact ggtacgaca 
      aagccaaag ttgggattt tcctccact ggggcgttt 
      actcagtgc ccagtttcg ggagcgaat ggttctgga 
      aattctggc aagatgaag aagaaccgt atgtgaact 
      ttatgcaag acaactatc cgcccgact tcacttatc 
      aagattttg caaaagatt tcaccgccg aattctacg 
      atcctgagc agtgggcgg acttgtttg ccaaatctg 
      gggccaaat atgttatcc tgaccagta aacatcacg 
      aaggttacg ccatgtggc cttcgaaat attcgtaca 
      gttggaatg ccaacgacg tggggccca atagagacc 
      tactaggag aacttgccg atgcaatta ggaacagta 
      cagacctac acttcggtc tgtaccact ctctgttcg 
      agtggtttc accccctgt atgacagcg atcgggata 
      ataatttca cgacttctg aatttgtcg actataaaa 
      ttattccag aaatgaaag aattggtcg aaacttacg 
      aaccggaag ttttatggt cggatggag atggagagg 
      ctttggata cgtactgga agtcactgg agtttctgg 
      cttggcttt acaatgaaa gccctgtca aggattttg 
      tcgtaacta acgacagat ggggaagta acgttgggt 
      gcaaacatg ggggtttct acacctgtg ctgatagat 
      acaaccctg gacatttgt tgccccaca agtgggaaa 
      actgcatga caattgaca aaaaatctt ggggttata 
      gaagaaatg ccgatctcg acgaattcc taacgacac 
      atgaactga ttaccacac ttgccgaaa ccattagtt 
      gtggaggga atattttaa taaacgttg gaccgacaa 
      aagccggaa caattatcc cacttttcc aggaacgtt 
      tactagatc ttggaactt ggttgtcca ttaacggtg 
      atgcaattt acgaaagta gcgcttgga gtgtacaaa 
      acgactctt tcacttctg gagtctggt acacagcta 
      aagatccag tggtgtatg caatagttt taaactggc 
      cgattgata atgttctgt acttgggct cggcaaagt 
      ctctctttg acaatggta aaaccactc aagttacga 
      tgcttggga atgaaaatg gggatcttg aggctgaag 
      ttgttgata cgactaatc tggtagctg tgacttttc 
      cggatagag caagtgttg aaagtgagt gggcttggg 
      tgctcaaaa tcacgcctg gttcttaat aattttttt 
      attgaaatg tgataataa agttgtagt gaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY