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Gene information
General information
TC002988 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 665407..667178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002988
    iBeetle screen iB_00491
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002988 RA
      MVQTVIFTL LLSCGLQFA SSAKYEPTW DSLDGRPLP 
      DWYDKAKVG IFLHWGVYS VPSFGSEWF WSHWESGDE 
      KTVEFIKKN YPPDWTYQD FAQQFTAEF YDPFMWVNL 
      FLKSGAKYI VLTSKHHEG YTLWPSKYS YSWNAKDVG 
      PHLDLLGEL AKRVKARGL HFGVYHSLF EWYHPLYLQ 
      DKANGWNSS NFVDTKIIP EMKELITTY KPEVLWSDG 
      EWEAPDTYW KSKEFLAWL YNESPVKDV IVVNDRWGN 
      DSLCTHGGF YTCSDRYNP GHLLPHKWE NAMTVDKPS 
      WGYRRNAVI SDILTPHEL ITTLVETVS CGGNILINV 
      GPTKEGTIS PIFQERLMQ LGSWLSLNG DAIYESKPW 
      TAQNDTSPS PIWYTAKNE TVYAIVLEW PSKNVVSLE 
      HPDIITFFT QAKPTVTLL GDNKNKTLS SDSSDTQVT 
      VTLPDKATV ASEWAWVLK MVPTKSI
    CDS sequence
    • >TC002988 RA
      atggtccaa accgttatc ttcacgttg ttactttcg 
      tgtggatta caatttgcc tcaagtgca aaatatgaa 
      ccaacttgg gacagcctc gacggtcgc cctttgccc 
      gactggtac gacaaagcc aaagtcggg attttcctc 
      cactggggt gtttactca gtgcccagt ttcgggagc 
      gaatggttt tggagtcac tgggaatcg ggtgacgaa 
      aaaactgtc gagtttatt aagaaaaac taccctcca 
      gactggact taccaagac tttgcccag caatttacc 
      gccgaattt tacgaccct ttcatgtgg gtgaacctc 
      tttctcaaa tccggggcc aagtacata gtgctaacg 
      agtaaacac cacgaaggc tacaccctg tggccgtcg 
      aaatattcc tacagttgg aacgccaaa gatgttggg 
      ccacacctt gacctttta ggtgagctt gcaaagcgt 
      gtcaaagcc agagggctc catttcgga gtttaccac 
      tcattgttc gaatggtac caccctctg tatctgcaa 
      gacaaagcc aatggctgg aacagctct aacttcgtt 
      gacacaaaa ataatcccg gaaatgaaa gaactgatc 
      accacttac aaaccggag gtgctttgg tctgacggt 
      gaatgggaa gcccctgat acctactgg aaatcaaag 
      gaattcctc gcatggctt tacaacgaa agccccgtc 
      aaagacgtt atcgtggtt aacgacaga tggggaaac 
      gactcgttg tgcacccat ggcggtttt tacacttgt 
      tctgataga tacaacccg ggacatttg ttgccacac 
      aagtgggaa aacgctatg actgtggac aaaccctca 
      tggggctac agaagaaat gcagtgatt tccgacatt 
      ctaactccc catgaattg ataacgaca cttgtagaa 
      accgttagt tgcggtgga aacattctt atcaatgtt 
      gggccgacc aaagaaggc acaatttcg ccgattttc 
      caggagcgg ttaatgcaa cttggttct tggttgtcc 
      cttaacggg gacgcaatc tacgagagt aagccctgg 
      actgcacaa aatgacacc tccccaagt cctatctgg 
      tacacagct aagaacgaa actgtctac gcgatagtg 
      ctagaatgg cccagtaaa aatgtagtg tccttagag 
      catccagac attatcacg tttttcacg caagcaaaa 
      ccaacggtg acgcttcta ggagataat aaaaataaa 
      actctttcg agtgattca agtgatact caagtcact 
      gtgacttta ccagacaaa gcgacagtt gcaagtgag 
      tgggcatgg gttcttaaa atggtacct acaaagtcg 
      atttaa
    mRNA sequence
    • >TC002988 RA
      tatgaaaca aacaagact cttacagat aagaaaaca 
      atattatat ttttgataa caaccgatc gctcgttga 
      gaacaagta gaaaacatt acaacaaac aacagtggt 
      tcaaacggt gcaatcatg gtccaaacc gttatcttc 
      acgttgtta ctttcgtgt ggattacaa tttgcctca 
      agtgcaaaa tatgaacca acttgggac agcctcgac 
      ggtcgccct ttgcccgac tggtacgac aaagccaaa 
      gtcgggatt ttcctccac tggggtgtt tactcagtg 
      cccagtttc gggagcgaa tggttttgg agtcactgg 
      gaatcgggt gacgaaaaa actgtcgag tttattaag 
      aaaaactac cctccagac tggacttac caagacttt 
      gcccagcaa tttaccgcc gaattttac gaccctttc 
      atgtgggtg aacctcttt ctcaaatcc ggggccaag 
      tacatagtg ctaacgagt aaacaccac gaaggctac 
      accctgtgg ccgtcgaaa tattcctac agttggaac 
      gccaaagat gttgggcca caccttgac cttttaggt 
      gagcttgca aagcgtgtc aaagccaga gggctccat 
      ttcggagtt taccactca ttgttcgaa tggtaccac 
      cctctgtat ctgcaagac aaagccaat ggctggaac 
      agctctaac ttcgttgac acaaaaata atcccggaa 
      atgaaagaa ctgatcacc acttacaaa ccggaggtg 
      ctttggtct gacggtgaa tgggaagcc cctgatacc 
      tactggaaa tcaaaggaa ttcctcgca tggctttac 
      aacgaaagc cccgtcaaa gacgttatc gtggttaac 
      gacagatgg ggaaacgac tcgttgtgc acccatggc 
      ggtttttac acttgttct gatagatac aacccggga 
      catttgttg ccacacaag tgggaaaac gctatgact 
      gtggacaaa ccctcatgg ggctacaga agaaatgca 
      gtgatttcc gacattcta actccccat gaattgata 
      acgacactt gtagaaacc gttagttgc ggtggaaac 
      attcttatc aatgttggg ccgaccaaa gaaggcaca 
      atttcgccg attttccag gagcggtta atgcaactt 
      ggttcttgg ttgtccctt aacggggac gcaatctac 
      gagagtaag ccctggact gcacaaaat gacacctcc 
      ccaagtcct atctggtac acagctaag aacgaaact 
      gtctacgcg atagtgcta gaatggccc agtaaaaat 
      gtagtgtcc ttagagcat ccagacatt atcacgttt 
      ttcacgcaa gcaaaacca acggtgacg cttctagga 
      gataataaa aataaaact ctttcgagt gattcaagt 
      gatactcaa gtcactgtg actttacca gacaaagcg 
      acagttgca agtgagtgg gcatgggtt cttaaaatg 
      gtacctaca aagtcgatt taaaatgta gctacaaca 
      aatttgaag aaaaatatg tattgtaac aaaattaga 
      tttttcctg aataaagaa ttacagaaa ac