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Gene information
General information
TC002983 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 746195..753380
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002983
    iBeetle screen iB_00489
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 488 adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 488
    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 488
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupation delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    pupal molt - (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002983 RA
      MSDGYFEKH QAHPYQNSK YSSSKSYGS TSERYERIR 
      DSPNGNYRS DSPDSQSPR DRDRSYQSK SSYLQKIRE 
      KERENRDYK MSRDKYSDC ARSPKDKRS RESEHRTNN 
      DRNDEKSIL HPIKMSQNL SSRDRKPVH NNSVDKRDD 
      RERVARVGD WSEHVSSSG KKYYYNCKT EVSQWEKPR 
      EWMERDRFR SRERDRERD SDRCYSRSG TRHEKHSNS 
      RAPNSSSST KEGTRDSAT GSRHWSSSS SRDDSDVPV 
      KDGRSRKHP DENVQSTQD MDISPGDST PTSEISYGN 
      SSSQQTAEP NTLGPVLLA NALPRLISH PIHMPSTPG 
      RPESSPQST PSMMPVPTT VTISNTPGP PTSLGSLPR 
      IMSQTTPTD NSDKNSHGS IGLQRQTVS GDSAVHTSL 
      SVDTNHTGT VVEGPPTPT HSETPDCSK VVSVPVSSP 
      PGNLSTLQG MSSSLQALR PQGPSITPS LANHYRDDL 
      VNHVRGWPA EILEKQAQK LAEEAHIMG SIQCSKVSA 
      ELKSARSIV RLTEIQATL QEQRILFLR QQIKELEEL 
      KSQNSFMSD DS
    CDS sequence
    • >TC002983 RA
      atgagcgat gggtacttc gagaagcac caagcccat 
      ccctatcag aactcgaag tatagttca tcgaagagt 
      tatggatcc actagtgaa aggtacgag agaattcgg 
      gattctcca aatggtaat tatcggtcg gacagccca 
      gattctcaa tctcccaga gaccgtgat cgatcttat 
      caatcaaag agctcctac ctacagaaa atacgtgaa 
      aaggagaga gaaaataga gactataaa atgtcaaga 
      gacaagtat tcagattgt gcccgatcg ccaaaggat 
      aagcgcagc agagaatct gagcacagg accaacaat 
      gacagaaac gacgagaag agcatttta catccaata 
      aaaatgtcc cagaacctg tcttcgagg gacaggaaa 
      ccagtacac aataattct gtggataag agggatgat 
      cgggaaagg gttgctaga gtaggtgat tggtcggag 
      catgtaagt tcgtcgggt aaaaaatat tattacaat 
      tgcaaaacc gaagtatcg caatgggag aaaccaagg 
      gaatggatg gaacgagac cgatttagg agtcgagaa 
      cgggacaga gaacgagac tcggatcgt tgttacagc 
      cgctccggc acgcggcat gagaaacat tccaactcg 
      cgagcaccg aacagcagt agcagtacg aaggaagga 
      acgagagat agcgcgacg ggaagtcgg cactggagc 
      agtagtagc agccgagac gacagcgac gttcccgtc 
      aaagatggc agatcgaga aagcatcca gatgagaac 
      gtgcagtcg acacaagac atggatatc tctcccggc 
      gatagcacg ccgacgtcc gagatatcc tacgggaat 
      agttcgagc cagcagacg gccgaacca aatacactg 
      ggacctgtt ttattggca aacgcattg cctcgatta 
      atttcacac cctattcac atgccctcg acgccggga 
      cgacctgaa tcgtcaccg cagagtaca ccctcgatg 
      atgcccgtg cccacgacc gtgacaata tcgaacacg 
      cctgggcct ccaacgagt cttggaagt ttaccccga 
      attatgtca cagactacg cctacagat aattctgat 
      aaaaactcg catggttct ataggtttg caaaggcag 
      actgtttct ggcgatagt gcagtgcat acttctcta 
      agtgtagac actaatcat acaggaact gtagttgaa 
      ggtccacct acacccaca cattctgag acgcctgat 
      tgttctaaa gtcgtctca gtacctgtt agtagtcca 
      cctggcaat ctctcgaca ctgcaggga atgtcaagc 
      tcgttacaa gctttaaga ccccaaggt ccttcaata 
      acaccgtca ttggctaac cattataga gatgattta 
      gttaaccat gttagggga tggccagca gaaatttta 
      gagaaacaa gcccaaaaa ttagcagaa gaagctcac 
      attatgggt agtatacaa tgttctaaa gtttccgct 
      gaactcaag tctgctagg tcaatagtt cgtctcaca 
      gaaattcag gccacatta caagaacag agaattttg 
      tttcttcgt caacaaatt aaagaattg gaagaatta 
      aaatcccaa aattcattt atgtctgat gattcttag
    mRNA sequence
    • >TC002983 RA
      ggtagacgg gacgttttc ttacgtcaa ccaatgaga 
      caacggaag aaattagga gcatttacc ttcagctgc 
      aaattgttc ccttatgtg acatggcgt ctggtagga 
      aagtcagtt cttgatcgg tttcaaacg tttttattg 
      tttttatga ccttttcat ccaaatcgg cttgctgtg 
      aaagccaca aacatttta tagagactt gcattgatg 
      gtaatgcat gcaaggaaa cctcaacgg atgagcgat 
      gggtacttc gagaagcac caagcccat ccctatcag 
      aactcgaag tatagttca tcgaagagt tatggatcc 
      actagtgaa aggtacgag agaattcgg gattctcca 
      aatggtaat tatcggtcg gacagccca gattctcaa 
      tctcccaga gaccgtgat cgatcttat caatcaaag 
      agctcctac ctacagaaa atacgtgaa aaggagaga 
      gaaaataga gactataaa atgtcaaga gacaagtat 
      tcagattgt gcccgatcg ccaaaggat aagcgcagc 
      agagaatct gagcacagg accaacaat gacagaaac 
      gacgagaag agcatttta catccaata aaaatgtcc 
      cagaacctg tcttcgagg gacaggaaa ccagtacac 
      aataattct gtggataag agggatgat cgggaaagg 
      gttgctaga gtaggtgat tggtcggag catgtaagt 
      tcgtcgggt aaaaaatat tattacaat tgcaaaacc 
      gaagtatcg caatgggag aaaccaagg gaatggatg 
      gaacgagac cgatttagg agtcgagaa cgggacaga 
      gaacgagac tcggatcgt tgttacagc cgctccggc 
      acgcggcat gagaaacat tccaactcg cgagcaccg 
      aacagcagt agcagtacg aaggaagga acgagagat 
      agcgcgacg ggaagtcgg cactggagc agtagtagc 
      agccgagac gacagcgac gttcccgtc aaagatggc 
      agatcgaga aagcatcca gatgagaac gtgcagtcg 
      acacaagac atggatatc tctcccggc gatagcacg 
      ccgacgtcc gagatatcc tacgggaat agttcgagc 
      cagcagacg gccgaacca aatacactg ggacctgtt 
      ttattggca aacgcattg cctcgatta atttcacac 
      cctattcac atgccctcg acgccggga cgacctgaa 
      tcgtcaccg cagagtaca ccctcgatg atgcccgtg 
      cccacgacc gtgacaata tcgaacacg cctgggcct 
      ccaacgagt cttggaagt ttaccccga attatgtca 
      cagactacg cctacagat aattctgat aaaaactcg 
      catggttct ataggtttg caaaggcag actgtttct 
      ggcgatagt gcagtgcat acttctcta agtgtagac 
      actaatcat acaggaact gtagttgaa ggtccacct 
      acacccaca cattctgag acgcctgat tgttctaaa 
      gtcgtctca gtacctgtt agtagtcca cctggcaat 
      ctctcgaca ctgcaggga atgtcaagc tcgttacaa 
      gctttaaga ccccaaggt ccttcaata acaccgtca 
      ttggctaac cattataga gatgattta gttaaccat 
      gttagggga tggccagca gaaatttta gagaaacaa 
      gcccaaaaa ttagcagaa gaagctcac attatgggt 
      agtatacaa tgttctaaa gtttccgct gaactcaag 
      tctgctagg tcaatagtt cgtctcaca gaaattcag 
      gccacatta caagaacag agaattttg tttcttcgt 
      caacaaatt aaagaattg gaagaatta aaatcccaa 
      aattcattt atgtctgat gattcttag tggatgaaa 
      atatcacaa gattagtgt taatagcta aacattttc 
      gttaagtgg aaagtatgc attattatt taattaatt 
      tataacttc agccggtgt atatttata tttccttga 
      atttgcctt caagttcat aacttgaca aggcctatt 
      ttggcattt tatttaatt tgttagata ctaaagact 
      atttctctt aggtagaat ttaatatgt gacctgtat 
      tataatggt tttatgttt tagatttag ataaattaa 
      tataatata gaaatagtt tttttttca acagtgtat 
      attttcatt tgatctttg ttttattat gatggcatg 
      ctgtacaaa gtgatttta ctggtcgaa gtactattt 
      atgtattta cgttgtatt tataaaaat atttgataa 
      aataaaatt atgctataa actaatcta tcttgtact 
      cgtcacata tgattttag atcatggga acagaactg 
      aacgaaaat gactcaatc aaatcaaat ggaaatata 
      cacaacaca gtaaaaagc acattgctc cttctgaat 
      ataaatatt ttaaagttt gtatttttt ccaaaaaaa 
      aaagaaaaa taaaaatct attaaatat cttgtgata 
      taaacactg ttcatgttt ctgttctat gttgcttta 
      atgtaagaa atttataaa atacaattg ttatattca 
      atataaata aagttacac taattttgt acatccaga 
      taattgaat atgacaact gtaggacga ttttataca 
      attatcttg tttacatct cttttgtat tatatttta 
      ttttcactt ttaaaatta taacttgta aaaaagtac 
      aacttttga cgcaaatcc ttagtctta atttccaca 
      ggattaagg ctcttcaga cgttttagc acagtttca 
      tttggctga ggaaatact ttgtatagt tgtctccat 
      gtatttgag tgtatgtct gttatatat aatcttata 
      aaattgaaa tgttccata caagtaaca ttaaaactt 
      tttaatcta tatggactt ttagttgta aagttattg 
      tcctccaca caaattttg taactaaga acaactgca 
      ggaatttca gatagtacc aggatctaa aaagaaact 
      aggtcaata catactcat gatggtttt ataactaat 
      gtaatttta ttaactaaa acaaaattt aacaaaact 
      tggctttat taagtataa attattgca taatttaaa 
      aagatacta gcgtagtag gaatgattt ggaacatta 
      aaaaatagg gtgctcctg tcagttgtt ttccattgt 
      aaacttcac attcaacat aaatatgta aaactaccc 
      aaacgaaaa taatttaaa caaaactag gccaagttc 
      ttgacaaat ataactaat aaaattata cattaaaaa 
      aataaaggc acaaatatg ctgaaaatt ccatccgtt 
      cgtaaaaag taacgcaac ctcgtgata aatgaatta 
      ggaataaca atagctgaa gtaaaaatg aaattttca 
      gcaaaacgt tttatttca tgtagaatt acaatattt 
      acacgaagc agtctaact ccttttaat acttctcac 
      ataaaaaga gcccaataa cggctcatt acgtaaaac 
      aacacaagt atttcagac aacaaaaat tattcattt 
      aaatatatc ctacgaata taattatac aaaatagat 
      gtgttgtta aaaaccaca cttttaaac aaatattta 
      caacttttt caatttgag gaactaagg agtgagcct 
      actttttga taagataac acagacaaa aactaaatt 
      aacaataaa aaataaata ctattgcat tgcaataat 
      tactagttc gatactcag caatccttg ataaacaac 
      ttggctgcc aaattcttc caacaaacc aaattacaa 
      cgtaaaaaa ataatctag tcttctacc atggacttt 
      tgcaataaa aacaataat tcgttttgt ctgtg