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Gene information
General information
TC002979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5422810..5425757
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002979
      iBeetle screen iB_00488

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
      Days post injection


      pupa lethality during eclosion - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      Pupal morphology
      elytra shortened - Number of animals showing the phenotype: 8

      Adult morphology
      wings shortened - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002979 RA
        MGFDINRFQ GDVDEELVC PICSGVLED PLQAPVCEH 
        AFCKSCITE WITRQPTCP VDRQSVTSA QLRPVPRIL 
        RNLLSRLCI NCDNAQYGC TRVLKLDSL SNHLEECEH 
        NPKRPLPCE KGCGLVIPK DELKDHNCV KELRALIQT 
        QQQKMNDFQ QEITEQRFL INEQKREMQ LLKEFMRAM 
        RISNPSMRA IADAMERDE VLRWSNFLQ RARVTRWGG 
        MISTPDEVL QRMIRRTLT EMGCPNHIL DDLMENCHE 
        RRWPPGLSS LETRQNNRR QYENYVCKR VPGKQAVLV 
        LACDNTHMG EDMMVEPGL VMIFAHGIE
      CDS sequence
      • >TC002979 RA
        atgggcttt gatattaat aggtttcaa ggagatgta 
        gatgaggaa ttggtgtgc cccatttgt tcgggagta 
        ctggaggac cccttgcag gcacctgtg tgcgagcat 
        gcattttgc aagtcctgc ataaccgaa tggatcaca 
        agacagccc acatgcccc gtggaccga caaagtgtc 
        acatcagcg caactgcgc ccagtgccc cgaatttta 
        cgaaacttg ctttctaga ctgtgtata aattgtgat 
        aacgctcag tatggctgt acgcgtgta ttaaaatta 
        gactctctc tcgaaccac cttgaagaa tgcgaacat 
        aatcctaag agaccttta ccgtgtgag aagggctgt 
        ggtttagtg attcctaaa gatgagttg aaagaccac 
        aattgcgtg aaagaactg cgagcgctg atacagacc 
        cagcagcag aaaatgaac gacttccag caggaaatc 
        accgagcag cgcttccta atcaacgag caaaagcgc 
        gaaatgcag ctgctgaag gagttcatg agggccatg 
        aggatatcg aacccttca atgagggca atagctgat 
        gcaatggag agagatgaa gtcttaagg tggagcaac 
        tttctccaa agggcgcga gtcacccga tggggaggg 
        atgatctcg acaccagat gaagttctt caaagaatg 
        attagaagg acgttaact gagatgggg tgccccaac 
        cacatatta gacgatcta atggaaaat tgtcacgag 
        aggaggtgg ccccccggc ttgagctcg ctggagacc 
        aggcaaaac aataggaga caatacgaa aactatgtt 
        tgtaaaaga gtaccgggc aagcaggct gtgctggta 
        ctggcgtgc gacaatacg catatgggc gaagacatg 
        atggtagag ccaggttta gttatgatt tttgctcac 
        ggtatagag taa
      mRNA sequence
      • >TC002979 RA
        acatatgat tttacaatt tataaataa atcgcagtg 
        ctaaaatcc gtgcaaatt gtgatgggt cgttactgt 
        taaaatggg ctttgatat taataggtt tcaaggaga 
        tgtagatga ggaattggt gtgccccat ttgttcggg 
        agtactgga ggacccctt gcaggcacc tgtgtgcga 
        gcatgcatt ttgcaagtc ctgcataac cgaatggat 
        cacaagaca gcccacatg ccccgtgga ccgacaaag 
        tgtcacatc agcgcaact gcgcccagt gccccgaat 
        tttacgaaa cttgctttc tagactgtg tataaattg 
        tgataacgc tcagtatgg ctgtacgcg tgtattaaa 
        attagactc tctctcgaa ccaccttga agaatgcga 
        acataatcc taagagacc tttaccgtg tgagaaggg 
        ctgtggttt agtgattcc taaagatga gttgaaaga 
        ccacaattg cgtgaaaga actgcgagc gctgataca 
        gacccagca gcagaaaat gaacgactt ccagcagga 
        aatcaccga gcagcgctt cctaatcaa cgagcaaaa 
        gcgcgaaat gcagctgct gaaggagtt catgagggc 
        catgaggat atcgaaccc ttcaatgag ggcaatagc 
        tgatgcaat ggagagaga tgaagtctt aaggtggag 
        caactttct ccaaagggc gcgagtcac ccgatgggg 
        agggatgat ctcgacacc agatgaagt tcttcaaag 
        aatgattag aaggacgtt aactgagat ggggtgccc 
        caaccacat attagacga tctaatgga aaattgtca 
        cgagaggag gtggccccc cggcttgag ctcgctgga 
        gaccaggca aaacaatag gagacaata cgaaaacta 
        tgtttgtaa aagagtacc gggcaagca ggctgtgct 
        ggtactggc gtgcgacaa tacgcatat gggcgaaga 
        catgatggt agagccagg tttagttat gatttttgc 
        tcacggtat agagtaacc ctctttgtt tttattttt 
        tttaattac attcctttt aaatacatt aatttacat 
        taagtttaa tcataatct ttctcactg ctttattat 
        aaaatctgg ggtctgtaa gtgccacaa attgttccg 
        ttctggtgg tgatgcagt caattttat caattagaa 
        ttcgtaaaa ttagaaaat tgacaggaa gtgcactaa 
        tgatacagg attattcag cttgaacaa attaactgt 
        tttgtaact tactgcaat gtgccatta ttcatgaac 
        caaaagtaa attaaggct gtggtctac tttattaaa 
        acattttca cccgtttac gtataaata gctttaatg 
        ggtcattta aacaccggt aaaaatgaa actaattcg 
        ccgcttgag gggctttaa acgtcgttt gtagaaaat 
        ttaattctc ttcaactgt ttttgttac gtttttctt 
        gtgtttttg attgtattc gcatgtaaa ttataaatt 
        taataaatt attcatgca ctttaaaaa ataataata 
        atgtaattt atactttca atgagagat ctaaccatt 
        aataattat tgactgtgt ctaaactcc cgggagcgt 
        cacgaatat tgaaagtga cgtcatcaa cgaccagga 
        tttttaatt gttttattg attttttgt attttacac 
        ataattttt gttgtaaat attatagca atccttaaa 
        ttttccaat tggaggaga agccaatca gtcaagtct 
        ttaataacg attgcaagg ccaaattta aaaaatact 
        tacatttta ttttttatt acacaaaaa gtttgtgtc 
        ttcgagcat catatatat atgatgagt gcctataat 
        aatagggag aattttttt gcagaccag tgttattga 
        acttacttt ttgcaatca agaaattat tataaacgt 
        tgactgcat catcaattt tgttccaga acgtgttca 
        tttgtcgat tttataatg cgtaaagtc atcaataat 
        tcctgccgc caggataat tgattactt ttgcatttt 
        caaagaatt tgctgttaa tattgattt ttcaacaat 
        aacggaaac gctgacgca gacttgaat gttggtgga 
        tgtgcggtt ttcccgttt tgtttcgtt ttagttatg 
        tatagttta atgatctga catttgatc tcaaaaatt 
        cacaacaaa gtcgttcct acatccgat cggatttgg 
        tctgacata acactagtc acgtcagaa atgtgattt 
        aatttctaa attttttga atggaggta tggaaatat 
        caaacacta aattctact cgaagctcc cctacgttt 
        tgaggacta gcaaaggaa ctcaaagag ctaaataca 
        tttgtgtag cttcttgca gttaatttt tgttagggt 
        tttgggggg tgcgatgtt tatttattt tgtaatgtg 
        attaatatt gaggcttaa atgtactta acttctgtt 
        gggcatttt gtagttctt atgtgctgc caattcttg 
        taataaaca agtaaattt ttatatgtt aatgtgatt 
        acgaccccg tttgtaaaa gtaaaacac gaattcgga 
        cacgagaaa taataacgc gattaaaac accgcctaa 
        aaacagtaa aattgcgga tcctacgtg ttcacgacc 
        ggcacttga atacccagg attcaagtt gagagttga 
        aacaggccg taaatttga tttgagaat tttttgcaa 
        tattccaga ctcgtccaa agcaattac actgaaaaa 
        caatgatat aacgtattc caacaatct taaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY