Status
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Gene information
General information
TC002976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5485473..5494258
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002976
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Comment: uterus size increased
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber rounded - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 1
    wings shortened - Number of animals showing the phenotype: 1
    wings not closed - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002976 RA
      MNNLEFVLK SNHRSEPGS GFWQLQLDR DLVDTISAI 
      YPEWFQNNM AEGGSPSTP RESPPKAVI DTSPLHIDQ 
      NKESLKVKL MLRRPFDQL VAQGIMPPH KTPAAFHGQ 
      RRQLERAKT GDMLKAKIQ QRPPRQELE RRHILEADP 
      GHVDPSLAE RQRMLKKAR LADQLNDQL SHRPGPLEL 
      IQKNILHTE EPIEQAVKT GRIPYKATS EGQLNRPQH 
      PQSYVTPED DSQSSEGDN IVSPGPSDV LETAAKSAG 
      IVVSLIPAT EGTVVVTTA TPVLNKDNS EIVFADLCR 
      SVAAPLLPQ VSASSPASL VSSTSTLSP LSSVASPVP 
      SVVSQPATP APPPPPPIV LTPRPIQSP AKSDAPGKD 
      KNRKKSKSK SAPKARTIK FHEYKGPPS AQKNSNNNN 
      NNGESSYDL LLQQQTLLL QFQLQLQHK YPQIILPAS 
      QKTNNETNN NNPLPSPSP STSSESSTP ARLTGRLED 
      MKVSDLKAE LKRRSLPVS GSKPQLIER LKPFTGSDS 
      LNQNISSVD STHSNSSMD QSHNSPQYQ ETGSPQGSV 
      KEEPAEQME ISDPRSPLP QQQIQEQFQ QLQQQIAQQ 
      QQNQENSQK SQEEIVREQ QRQIEELQR ELTLSKLRL 
      QEVTRVEPK AQLLALQKH LQARQQQQQ HQQQQQIAL 
      QMQQLQALQ ARQAHVNEE QQRIQQQQH VAFQNQKNM 
      TGGLVLNGA DAALVFNLM QGKAKVVNG HVRTNSLPS 
      FLNSIVPTP VIAAPDVKP EYIEDVKPP PPLYEEATK 
      QINKKNNVK SQTVDDVLE ILIKNGELP PSAAQDPST 
      PGSAGTKTT EPVFPNSPE AALGLDPTE ILDTLDNLD 
      NMDFTQFVM ELGDNQQHS DNNNMQSDH EQMSVPMDT 
      DDWLESLCV ENGHNGACT APLASQEAD LVGYDPLLG 
      ITQDPFDPF NLEEFQSPA DLTASLSWD KVDYAA
    CDS sequence
    • >TC002976 RA
      atgaacaac ttggagttt gttttaaag tcaaatcac 
      cggagtgag ccgggctct gggttttgg cagctccaa 
      ttagatcgc gacttggtt gataccatc tccgctatt 
      tacccggag tggtttcag aacaatatg gccgaggga 
      ggatcgcca tcgactcct agggagtca ccgccgaag 
      gcggtgatc gatacatct ccactccac atcgatcag 
      aataaagaa tccctcaag gtgaagttg atgctacgg 
      cggccgttc gaccagctg gtagcgcaa ggaatcatg 
      cctcctcac aagacgccg gcggccttc cacggccag 
      cggcggcag ctggagcgg gcgaagacc ggcgacatg 
      ctcaaggcg aagatccag cagcggcct ccgcggcaa 
      gaactcgaa aggcggcat attctcgag gcggatcca 
      ggtcacgta gatcccagt ttggcggaa cgccaacga 
      atgctgaaa aaagcgcgc ttagcggac caactcaac 
      gaccaacta tcacacaga cccggtccc cttgaactc 
      atccagaag aacatcctc cacacggag gagcccatc 
      gagcaggcc gtgaagacc ggccggata ccgtacaag 
      gccaccagc gagggccaa ctcaaccgc cctcagcac 
      ccccagagt tatgtcact ccggaggac gactcgcaa 
      agttccgaa ggcgataat attgtcagt ccaggacct 
      agtgatgtt ttagagacc gccgcgaaa tcggcgggt 
      atcgtggta tcgctcatt cccgccacc gaaggcacg 
      gtggtggtt acgactgcc accccagtg ttaaacaaa 
      gacaacagt gaaatagtg ttcgctgat ttgtgcaga 
      tcggtcgct gcgcctctt ctgccccaa gtatcggcc 
      tccagtccg gcgtcccta gtgtcttcc acgtccact 
      cttagtccc ttatcatca gtcgctagt cctgtgcct 
      tcggttgtc tcacaaccg gccacacca gcaccaccc 
      ccgccacca cctatagta cttacacct agaccaatc 
      caatcacct gccaaaagc gatgctccc ggcaaggac 
      aagaacaga aaaaaatcc aagtcaaaa tcggccccc 
      aaagcacgc accattaaa ttccatgaa tataaggga 
      ccaccgagt gcccaaaag aactcaaac aataacaac 
      aacaacgga gaaagctcc tacgatctc ttattacag 
      caacaaacc cttttactg cagtttcaa ctgcagttg 
      cagcacaaa tacccccaa attatacta ccagcttcc 
      caaaaaaca aacaacgaa acaaataac aataacccc 
      ctaccgtcc ccatcacca tcaacttcg tcagaatca 
      tcaaccccc gcacgcctt acaggtcgc ttagaagac 
      atgaaagtc agcgatttg aaagccgaa cttaaacgc 
      cgcagcttg ccagtctcg ggctccaaa ccccaatta 
      atcgagaga ttaaaacca ttcacagga tccgattca 
      ttaaatcag aatatttcg tcagtggat tcaacccac 
      agcaattcg agcatggac cagtcacac aactcccct 
      caataccaa gaaaccggt tcacctcaa ggttcggtg 
      aaggaagaa ccggccgaa cagatggaa atttcggat 
      cctagaagt cctttacca caacaacaa atccaggaa 
      caattccaa caattgcaa caacaaatc gctcaacaa 
      caacaaaat caagaaaac tcacaaaaa tcccaagaa 
      gaaatcgta cgagagcag caaagacaa atcgaggag 
      ttgcaacgc gagttgact ctctcgaag ttgcggctc 
      caggaagtg actcgtgtc gagcccaaa gcccagttg 
      ttggccctg cagaagcac ctgcaggcg cgacagcag 
      caacaacag caccaacaa cagcaacaa atcgctttg 
      cagatgcaa caattgcag gcgttgcaa gcgcgacaa 
      gctcacgtt aacgaagaa caacaaagg atacaacaa 
      cagcaacat gtggcgttc cagaaccag aaaaacatg 
      actggtggg ttggttctg aacggtgct gatgcggct 
      ctagtgttt aacctaatg caaggcaaa gcgaaagtg 
      gtcaatggg catgtgcga actaattcg ttaccaagt 
      ttcttgaat tccattgtt ccaacgccg gtaatagcg 
      gcacctgac gttaaaccg gaatatatt gaagatgtg 
      aagccaccg ccgcctctc tacgaagaa gccacgaag 
      caaatcaac aagaagaac aacgtcaag agtcagact 
      gttgacgat gtgttggag attcttatc aaaaacggg 
      gaattgcct ccgagtgca gctcaagac ccgtcgact 
      ccgggctcc gccggaacg aagacaacc gagccggtg 
      ttcccgaac agtccggag gcggctctg ggtctggac 
      ccgaccgag attttggac actttggac aacctggac 
      aacatggac ttcacccag ttcgtgatg gagctgggc 
      gacaaccag cagcattcg gacaacaac aacatgcag 
      agtgaccac gagcagatg tcggtgccg atggacacg 
      gacgattgg ttggagtcg ctgtgcgtc gagaacgga 
      cacaatggt gcgtgtacg gcgccgctg gcgtcgcaa 
      gaggccgac ctggtgggc tatgacccc ctgctcggc 
      atcactcag gatcccttc gatccgttt aacttagaa 
      gagttccag tcacctgcc gatttgacc gcgtcgctt 
      tcttgggac aaagtcgac tatgcagcg tag
    mRNA sequence
    • >TC002976 RA
      aattgtcag gaagaattg tgatgatgt tctggcaga 
      atgacttca gaagcgtgc caaattgca cttatcgta 
      acgccaatc attgtattt ttctagcga catatatgc 
      taacttcac gtgtttttc attaatgat aataaatga 
      atggaaagc gggcaaaca cgtgcctaa attaaaggg 
      ggtttctgg acttggcag cttttttaa ctgaaaaga 
      gattatttt gacaaagca aaatgtgac gaaatgatt 
      ttcgaagaa agtttggaa atatctgta gtcctggaa 
      aattttcaa gagagatga agagttaat aaattgtaa 
      aaagtttcg tcgtgtgta ataaagcgc agcatcatc 
      aataactaa acgtgtcat cgtcgggac tttgtatcg 
      ccaatcgga tgtcgaatt aacagctgt tgaaaaatg 
      atttcgcaa taaatattt gaagctgaa agtgatagt 
      tatgtgggt ccataattt atccggggc cagtgctct 
      tccacgaaa ttggtgata cccgacgga aatttttcc 
      cgcaaattg ggatagact atttcagga ttctagttc 
      tttgggtta gtgacttcg cattccagg atgattggt 
      taatttaca acagttgtt ttcagcatc agcagttcc 
      attataaga ggaaacgtc aggaggtta gacacacgt 
      atggaatag ccaactgac aagacccga cttaatggc 
      tctgctggg ctatttatt ttgatacta cagccatgc 
      gacttcaat taagttttg cgccaagtg tgattagcg 
      tttgttttc atttaaatg atttcggga tgctataat 
      aagcaggtg tcattaaga tatttaacg taaaatttt 
      gtttttgtg tatgtcatc ttagtgttg ctctctatt 
      tttatggat ctttgacct atgcatttc tttgataaa 
      cctaaatgt ttatttgca atgcacgta tattgaatt 
      gtggtcttg tggtgactg aagttaggt gttttgtgc 
      gtttttgta tagatttat ggggaaatg aacaacttg 
      gagtttgtt ttaaagtca aatcaccgg agtgagccg 
      ggctctggg ttttggcag ctccaatta gatcgcgac 
      ttggttgat accatctcc gctatttac ccggagtgg 
      tttcagaac aatatggcc gagggagga tcgccatcg 
      actcctagg gagtcaccg ccgaaggcg gtgatcgat 
      acatctcca ctccacatc gatcagaat aaagaatcc 
      ctcaaggtg aagttgatg ctacggcgg ccgttcgac 
      cagctggta gcgcaagga atcatgcct cctcacaag 
      acgccggcg gccttccac ggccagcgg cggcagctg 
      gagcgggcg aagaccggc gacatgctc aaggcgaag 
      atccagcag cggcctccg cggcaagaa ctcgaaagg 
      cggcatatt ctcgaggcg gatccaggt cacgtagat 
      cccagtttg gcggaacgc caacgaatg ctgaaaaaa 
      gcgcgctta gcggaccaa ctcaacgac caactatca 
      cacagaccc ggtcccctt gaactcatc cagaagaac 
      atcctccac acggaggag cccatcgag caggccgtg 
      aagaccggc cggataccg tacaaggcc accagcgag 
      ggccaactc aaccgccct cagcacccc cagagttat 
      gtcactccg gaggacgac tcgcaaagt tccgaaggc 
      gataatatt gtcagtcca ggacctagt gatgtttta 
      gagaccgcc gcgaaatcg gcgggtatc gtggtatcg 
      ctcattccc gccaccgaa ggcacggtg gtggttacg 
      actgccacc ccagtgtta aacaaagac aacagtgaa 
      atagtgttc gctgatttg tgcagatcg gtcgctgcg 
      cctcttctg ccccaagta tcggcctcc agtccggcg 
      tccctagtg tcttccacg tccactctt agtccctta 
      tcatcagtc gctagtcct gtgccttcg gttgtctca 
      caaccggcc acaccagca ccacccccg ccaccacct 
      atagtactt acacctaga ccaatccaa tcacctgcc 
      aaaagcgat gctcccggc aaggacaag aacagaaaa 
      aaatccaag tcaaaatcg gcccccaaa gcacgcacc 
      attaaattc catgaatat aagggacca ccgagtgcc 
      caaaagaac tcaaacaat aacaacaac aacggagaa 
      agctcctac gatctctta ttacagcaa caaaccctt 
      ttactgcag tttcaactg cagttgcag cacaaatac 
      ccccaaatt atactacca gcttcccaa aaaacaaac 
      aacgaaaca aataacaat aacccccta ccgtcccca 
      tcaccatca acttcgtca gaatcatca acccccgca 
      cgccttaca ggtcgctta gaagacatg aaagtcagc 
      gatttgaaa gccgaactt aaacgccgc agcttgcca 
      gtctcgggc tccaaaccc caattaatc gagagatta 
      aaaccattc acaggatcc gattcatta aatcagaat 
      atttcgtca gtggattca acccacagc aattcgagc 
      atggaccag tcacacaac tcccctcaa taccaagaa 
      accggttca cctcaaggt tcggtgaag gaagaaccg 
      gccgaacag atggaaatt tcggatcct agaagtcct 
      ttaccacaa caacaaatc caggaacaa ttccaacaa 
      ttgcaacaa caaatcgct caacaacaa caaaatcaa 
      gaaaactca caaaaatcc caagaagaa atcgtacga 
      gagcagcaa agacaaatc gaggagttg caacgcgag 
      ttgactctc tcgaagttg cggctccag gaagtgact 
      cgtgtcgag cccaaagcc cagttgttg gccctgcag 
      aagcacctg caggcgcga cagcagcaa caacagcac 
      caacaacag caacaaatc gctttgcag atgcaacaa 
      ttgcaggcg ttgcaagcg cgacaagct cacgttaac 
      gaagaacaa caaaggata caacaacag caacatgtg 
      gcgttccag aaccagaaa aacatgact ggtgggttg 
      gttctgaac ggtgctgat gcggctcta gtgtttaac 
      ctaatgcaa ggcaaagcg aaagtggtc aatgggcat 
      gtgcgaact aattcgtta ccaagtttc ttgaattcc 
      attgttcca acgccggta atagcggca cctgacgtt 
      aaaccggaa tatattgaa gatgtgaag ccaccgccg 
      cctctctac gaagaagcc acgaagcaa atcaacaag 
      aagaacaac gtcaagagt cagactgtt gacgatgtg 
      ttggagatt cttatcaaa aacggggaa ttgcctccg 
      agtgcagct caagacccg tcgactccg ggctccgcc 
      ggaacgaag acaaccgag ccggtgttc ccgaacagt 
      ccggaggcg gctctgggt ctggacccg accgagatt 
      ttggacact ttggacaac ctggacaac atggacttc 
      acccagttc gtgatggag ctgggcgac aaccagcag 
      cattcggac aacaacaac atgcagagt gaccacgag 
      cagatgtcg gtgccgatg gacacggac gattggttg 
      gagtcgctg tgcgtcgag aacggacac aatggtgcg 
      tgtacggcg ccgctggcg tcgcaagag gccgacctg 
      gtgggctat gaccccctg ctcggcatc actcaggat 
      cccttcgat ccgtttaac ttagaagag ttccagtca 
      cctgccgat ttgaccgcg tcgctttct tgggacaaa 
      gtcgactat gcagcgtag tgcgcgtgc gcgagtctc 
      catttgcgg tgccgctta ttccagtga tgtgcgatt 
      ccactcggt aagaaattg acattccac taacggccg 
      ccaagatcg ccagagggc gcgggacca gggcggccc 
      caatcggcc agggaacac atcaacaga tgatattgt 
      acatatata cttgtagtg gtagataac gcttagggt 
      ttacaaatt tcgtaagac acgaggttt taatttatt 
      tcaaggcgg cgatgagac tgaggtttt tttctaaaa 
      aaagaagct gcaaaaata gattttgaa atattccat 
      tttctgtga aagttgctc attggaacg aaatgacaa 
      tttcattta acgcaccaa ttggatcag ttatgtgcc 
      tcaggtagc aagggtttt cttttttac gtactttac 
      tgtattttt atagggttg aaaggttga atgtctgaa 
      taagtggtt ttatttggg attaggtaa ggtgtactt 
      tttatgtaa taaattgcc ttgatttat atgtatatt 
      ttgcatttg aagtatcgt gtatttatt aaaatattt 
      taggacgtt tgctaaaat tgattgtga tttttcttg 
      tcttttcgc cgctgtcat tgattcttg gaatttttt 
      acaaccgag tgtcaatgt ctgactacc tgtacagat 
      tgttttctg acaaagcct ttaattgtt aaattattt 
      cgaatgtta gacatttat tttgttgac tatttgtat 
      ttaggcagc ccgtgctcg gctgcaaca ggaatgtcg 
      cgctcttag attgcctaa tttattgta tgaaataag 
      aacttgaga tgccttatg atgattata cattgcctc 
      cttgtgttt gaatatgtt tcaacccat actgttcga 
      gggaaacat tttacaacg gctgcaata ttttataaa 
      cagtatgcg ggtgaaatg cttcattca taatgtaat 
      aaaaatgta attaatca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY