General information | |||
TC002972 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5523951..5526328 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002972 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00486 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC002972 RA
MSTLKTVWN CMFGPRLIK IYGNEGPVE KFYQPHSFE KWGDQVIHS LYVIWKIGV YTSPFLVGI LYQRGYLTV EGLGPFTKL VTSISVILI VSYCCKGLG RFSNPTYLN FLSTLEAAR KELTPETKH QLSLYDFEF HSWPVEFDM RTVRKDTTK TFINKPILH QNVLQYLTQ IPFRIIAYV AIHTFGIRL IYPGSIGII QMILEQSLL QGRSRLIEH NQGERFKVR TCDNNDIDT MFIDRRKST QNGNTLVIC SEGNAGFYE IGIMITAIE AGYSTLGWN HPGFGGSTG KPFPTQEQN AVDAVLQFA IYKLGFKPE NIILFGWSI GGYSTSWAA MNYPDVRGV IVDATFDDI LPLAINHMP SWWESIVKL AIREYVNLN VFEQLAKYP GPVLIIRRT EDEVICLRE NELSSNRGN DLLIKLLRV RYPYIFEDR QIDVLKQYL SVAFASQDL FLKKYNVNE NVCNSLLQT YISEYSKSF PMKIGEEFQ DQDKNQMAL FLARKYMKD FKATHCTNL PAEMFQPPW DVNVESDFV FT
- >TC002972 RA
atgtcaaca ctaaaaaca gtttggaat tgcatgttt gggccaaga ttaatcaaa atatacgga aacgagggc cctgtcgag aagttctat caacctcac agtttcgaa aaatggggc gatcaagtg atacattct ttgtacgtt atttggaaa atcggagtg tacacgtcc ccgttctta gtagggata ctataccag cgaggctat ttgactgtt gagggtttg gggccattc acaaaatta gttactagt ataagtgtt attctgata gtttcttac tgttgcaaa ggtttgggt aggttcagt aacccaact tacttgaac tttttgtca acactggaa gcagctagg aaggaattg acccctgaa acaaaacac cagctctct ttgtatgat tttgaattt cacagctgg cctgtagag tttgatatg cgtactgtg agaaaggac accacaaag acattcatt aataaacca attcttcac caaaacgta ttacaatat ctcacgcaa atacctttc cgaattatc gcttatgtc gcaattcac actttcggt attagatta atataccca ggctccatt gggatcatt caaatgata ctggagcag agccttctt caaggcagg tctaggctg attgaacac aaccaagga gaacgattc aaagtgcgc acttgtgac aacaacgac attgatact atgttcata gacagacgc aagagtacc caaaatggc aatactttg gtcatttgt tccgaaggc aatgcaggc ttttacgaa attggcatt atgataaca gctatagaa gcaggctac tcaacgctt ggatggaac catccaggc tttggcggt agtactggc aagccattc ccaacacaa gaacaaaat gcagttgac gctgtacta caattcgcc atttataaa ctaggtttt aagcctgaa aatatcata ctgtttggg tggagcatt gggggctac tcaacgtcg tgggcggcc atgaattat cctgatgtg agaggagtg attgttgat gctactttc gatgatatt cttccactg gccataaat cacatgcca tcttggtgg gaatccatc gtcaagcta gctattaga gaatacgta aacttgaac gtctttgaa cagcttgcg aaatatcca ggccctgtt cttatcatt aggcgaacg gaagatgaa gttatctgt ctaagagaa aacgaattg tcttcaaat cgcggaaac gacttatta atcaaattg ttacgtgtg agatatccc tacattttt gaagacagg caaatcgat gtattgaaa caatatcta tctgtcgcc ttcgcttct caagacttg tttttgaaa aaatataac gtgaacgaa aatgtatgc aattcactt ttgcaaact tacatttcc gagtattcg aaatctttc cccatgaaa attggggag gagttccag gatcaagac aagaaccag atggcgctc tttttggca aggaaatac atgaaagat ttcaaagcc acacattgc actaatcta ccggctgag atgtttcaa ccaccttgg gacgtcaac gttgagtct gattttgtt ttcacttaa
- >TC002972 RA
ccgcccctg tttctgctg tcaattgta aattgtgac gtcagacgt tacatttac aaacaattt tttaaaggt gtaaaatga aatgtaaat acttttgat atagtggaa tccgtcgtt aaacgtaca ttaagtgtt agaacttgc catattcat tattaaata gaaaattag caccttctt gcaactttc accacgaaa cctaaccat aaaattatc gcaaaatgt caacactaa aaacagttt ggaattgca tgtttgggc caagattaa tcaaaatat acggaaacg agggccctg tcgagaagt tctatcaac ctcacagtt tcgaaaaat ggggcgatc aagtgatac attctttgt acgttattt ggaaaatcg gagtgtaca cgtccccgt tcttagtag ggatactat accagcgag gctatttga ctgttgagg gtttggggc cattcacaa aattagtta ctagtataa gtgttattc tgatagttt cttactgtt gcaaaggtt tgggtaggt tcagtaacc caacttact tgaactttt tgtcaacac tggaagcag ctaggaagg aattgaccc ctgaaacaa aacaccagc tctctttgt atgattttg aatttcaca gctggcctg tagagtttg atatgcgta ctgtgagaa aggacacca caaagacat tcattaata aaccaattc ttcaccaaa acgtattac aatatctca cgcaaatac ctttccgaa ttatcgctt atgtcgcaa ttcacactt tcggtatta gattaatat acccaggct ccattggga tcattcaaa tgatactgg agcagagcc ttcttcaag gcaggtcta ggctgattg aacacaacc aaggagaac gattcaaag tgcgcactt gtgacaaca acgacattg atactatgt tcatagaca gacgcaaga gtacccaaa atggcaata ctttggtca tttgttccg aaggcaatg caggctttt acgaaattg gcattatga taacagcta tagaagcag gctactcaa cgcttggat ggaaccatc caggctttg gcggtagta ctggcaagc cattcccaa cacaagaac aaaatgcag ttgacgctg tactacaat tcgccattt ataaactag gttttaagc ctgaaaata tcatactgt ttgggtgga gcattgggg gctactcaa cgtcgtggg cggccatga attatcctg atgtgagag gagtgattg ttgatgcta ctttcgatg atattcttc cactggcca taaatcaca tgccatctt ggtgggaat ccatcgtca agctagcta ttagagaat acgtaaact tgaacgtct ttgaacagc ttgcgaaat atccaggcc ctgttctta tcattaggc gaacggaag atgaagtta tctgtctaa gagaaaacg aattgtctt caaatcgcg gaaacgact tattaatca aattgttac gtgtgagat atccctaca tttttgaag acaggcaaa tcgatgtat tgaaacaat atctatctg tcgccttcg cttctcaag acttgtttt tgaaaaaat ataacgtga acgaaaatg tatgcaatt cacttttgc aaacttaca tttccgagt attcgaaat ctttcccca tgaaaattg gggaggagt tccaggatc aagacaaga accagatgg cgctctttt tggcaagga aatacatga aagatttca aagccacac attgcacta atctaccgg ctgagatgt ttcaaccac cttgggacg tcaacgttg agtctgatt ttgttttca cttaaaatg tgaaggaat tggtgataa gaggtttta aaatgtcct tggtatttg taagatttt atgaaaata ttttggtgt aatatgaaa taaatatgt aaaaatgtg acggttcta gttaagttt tgttagtaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
