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Gene information
General information
TC002972 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5523951..5526328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002972
    iBeetle screen iB_00486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    TR: 47118 potentially confused/crosscontaminated with : iB # (comma separated) 00502
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002972 RA
      MSTLKTVWN CMFGPRLIK IYGNEGPVE KFYQPHSFE 
      KWGDQVIHS LYVIWKIGV YTSPFLVGI LYQRGYLTV 
      EGLGPFTKL VTSISVILI VSYCCKGLG RFSNPTYLN 
      FLSTLEAAR KELTPETKH QLSLYDFEF HSWPVEFDM 
      RTVRKDTTK TFINKPILH QNVLQYLTQ IPFRIIAYV 
      AIHTFGIRL IYPGSIGII QMILEQSLL QGRSRLIEH 
      NQGERFKVR TCDNNDIDT MFIDRRKST QNGNTLVIC 
      SEGNAGFYE IGIMITAIE AGYSTLGWN HPGFGGSTG 
      KPFPTQEQN AVDAVLQFA IYKLGFKPE NIILFGWSI 
      GGYSTSWAA MNYPDVRGV IVDATFDDI LPLAINHMP 
      SWWESIVKL AIREYVNLN VFEQLAKYP GPVLIIRRT 
      EDEVICLRE NELSSNRGN DLLIKLLRV RYPYIFEDR 
      QIDVLKQYL SVAFASQDL FLKKYNVNE NVCNSLLQT 
      YISEYSKSF PMKIGEEFQ DQDKNQMAL FLARKYMKD 
      FKATHCTNL PAEMFQPPW DVNVESDFV FT
    CDS sequence
    • >TC002972 RA
      atgtcaaca ctaaaaaca gtttggaat tgcatgttt 
      gggccaaga ttaatcaaa atatacgga aacgagggc 
      cctgtcgag aagttctat caacctcac agtttcgaa 
      aaatggggc gatcaagtg atacattct ttgtacgtt 
      atttggaaa atcggagtg tacacgtcc ccgttctta 
      gtagggata ctataccag cgaggctat ttgactgtt 
      gagggtttg gggccattc acaaaatta gttactagt 
      ataagtgtt attctgata gtttcttac tgttgcaaa 
      ggtttgggt aggttcagt aacccaact tacttgaac 
      tttttgtca acactggaa gcagctagg aaggaattg 
      acccctgaa acaaaacac cagctctct ttgtatgat 
      tttgaattt cacagctgg cctgtagag tttgatatg 
      cgtactgtg agaaaggac accacaaag acattcatt 
      aataaacca attcttcac caaaacgta ttacaatat 
      ctcacgcaa atacctttc cgaattatc gcttatgtc 
      gcaattcac actttcggt attagatta atataccca 
      ggctccatt gggatcatt caaatgata ctggagcag 
      agccttctt caaggcagg tctaggctg attgaacac 
      aaccaagga gaacgattc aaagtgcgc acttgtgac 
      aacaacgac attgatact atgttcata gacagacgc 
      aagagtacc caaaatggc aatactttg gtcatttgt 
      tccgaaggc aatgcaggc ttttacgaa attggcatt 
      atgataaca gctatagaa gcaggctac tcaacgctt 
      ggatggaac catccaggc tttggcggt agtactggc 
      aagccattc ccaacacaa gaacaaaat gcagttgac 
      gctgtacta caattcgcc atttataaa ctaggtttt 
      aagcctgaa aatatcata ctgtttggg tggagcatt 
      gggggctac tcaacgtcg tgggcggcc atgaattat 
      cctgatgtg agaggagtg attgttgat gctactttc 
      gatgatatt cttccactg gccataaat cacatgcca 
      tcttggtgg gaatccatc gtcaagcta gctattaga 
      gaatacgta aacttgaac gtctttgaa cagcttgcg 
      aaatatcca ggccctgtt cttatcatt aggcgaacg 
      gaagatgaa gttatctgt ctaagagaa aacgaattg 
      tcttcaaat cgcggaaac gacttatta atcaaattg 
      ttacgtgtg agatatccc tacattttt gaagacagg 
      caaatcgat gtattgaaa caatatcta tctgtcgcc 
      ttcgcttct caagacttg tttttgaaa aaatataac 
      gtgaacgaa aatgtatgc aattcactt ttgcaaact 
      tacatttcc gagtattcg aaatctttc cccatgaaa 
      attggggag gagttccag gatcaagac aagaaccag 
      atggcgctc tttttggca aggaaatac atgaaagat 
      ttcaaagcc acacattgc actaatcta ccggctgag 
      atgtttcaa ccaccttgg gacgtcaac gttgagtct 
      gattttgtt ttcacttaa
    mRNA sequence
    • >TC002972 RA
      ccgcccctg tttctgctg tcaattgta aattgtgac 
      gtcagacgt tacatttac aaacaattt tttaaaggt 
      gtaaaatga aatgtaaat acttttgat atagtggaa 
      tccgtcgtt aaacgtaca ttaagtgtt agaacttgc 
      catattcat tattaaata gaaaattag caccttctt 
      gcaactttc accacgaaa cctaaccat aaaattatc 
      gcaaaatgt caacactaa aaacagttt ggaattgca 
      tgtttgggc caagattaa tcaaaatat acggaaacg 
      agggccctg tcgagaagt tctatcaac ctcacagtt 
      tcgaaaaat ggggcgatc aagtgatac attctttgt 
      acgttattt ggaaaatcg gagtgtaca cgtccccgt 
      tcttagtag ggatactat accagcgag gctatttga 
      ctgttgagg gtttggggc cattcacaa aattagtta 
      ctagtataa gtgttattc tgatagttt cttactgtt 
      gcaaaggtt tgggtaggt tcagtaacc caacttact 
      tgaactttt tgtcaacac tggaagcag ctaggaagg 
      aattgaccc ctgaaacaa aacaccagc tctctttgt 
      atgattttg aatttcaca gctggcctg tagagtttg 
      atatgcgta ctgtgagaa aggacacca caaagacat 
      tcattaata aaccaattc ttcaccaaa acgtattac 
      aatatctca cgcaaatac ctttccgaa ttatcgctt 
      atgtcgcaa ttcacactt tcggtatta gattaatat 
      acccaggct ccattggga tcattcaaa tgatactgg 
      agcagagcc ttcttcaag gcaggtcta ggctgattg 
      aacacaacc aaggagaac gattcaaag tgcgcactt 
      gtgacaaca acgacattg atactatgt tcatagaca 
      gacgcaaga gtacccaaa atggcaata ctttggtca 
      tttgttccg aaggcaatg caggctttt acgaaattg 
      gcattatga taacagcta tagaagcag gctactcaa 
      cgcttggat ggaaccatc caggctttg gcggtagta 
      ctggcaagc cattcccaa cacaagaac aaaatgcag 
      ttgacgctg tactacaat tcgccattt ataaactag 
      gttttaagc ctgaaaata tcatactgt ttgggtgga 
      gcattgggg gctactcaa cgtcgtggg cggccatga 
      attatcctg atgtgagag gagtgattg ttgatgcta 
      ctttcgatg atattcttc cactggcca taaatcaca 
      tgccatctt ggtgggaat ccatcgtca agctagcta 
      ttagagaat acgtaaact tgaacgtct ttgaacagc 
      ttgcgaaat atccaggcc ctgttctta tcattaggc 
      gaacggaag atgaagtta tctgtctaa gagaaaacg 
      aattgtctt caaatcgcg gaaacgact tattaatca 
      aattgttac gtgtgagat atccctaca tttttgaag 
      acaggcaaa tcgatgtat tgaaacaat atctatctg 
      tcgccttcg cttctcaag acttgtttt tgaaaaaat 
      ataacgtga acgaaaatg tatgcaatt cacttttgc 
      aaacttaca tttccgagt attcgaaat ctttcccca 
      tgaaaattg gggaggagt tccaggatc aagacaaga 
      accagatgg cgctctttt tggcaagga aatacatga 
      aagatttca aagccacac attgcacta atctaccgg 
      ctgagatgt ttcaaccac cttgggacg tcaacgttg 
      agtctgatt ttgttttca cttaaaatg tgaaggaat 
      tggtgataa gaggtttta aaatgtcct tggtatttg 
      taagatttt atgaaaata ttttggtgt aatatgaaa 
      taaatatgt aaaaatgtg acggttcta gttaagttt 
      tgttagtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY