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Gene information
General information
TC002966 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5683091..5689198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002966
    iBeetle screen iB_00485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002966 RA
      MQDSLEDYI RISRNCKEG PTQVKKEVL ITAQIKKEC 
      DAKALQILQ FAIEGKMRS DALFKCLPY INQSHYQDI 
      VEERAISKL CGYPLCSKK IPDMPKKQF YISTKNNKV 
      YDITDRKNY CSNFCYRAS LHIKGQIDT SPLWLRKYQ 
      EKAHYTLLN KSEKGLPGE MIDQGVIKP AVEPYFTSV 
      SSFTQMSLD EAEELELNP QEEEKIKQK KKPRKYQRN 
      AMKTINENE EEEQVESGE KMDEMKESV EILNKENKN 
      TPKIKDKPL AQTKQTNAT DVESHIYKC IKEWLTLET 
      FMFVHGEEK VKEKLDSQK LSHYFDALK ISQLQVEQQ 
      KRYIDICRK LHLRELAEE KFDKAVVGN QALKPIPDY 
      RQLKEESRE IDLKVKCFY SGEIHIQED KNFPSKKED 
      TTNEEEEQP AVLPLVDVN SQNALRRKI FLNSLNKTV 
      QTLFQTLRI SPSLVLSDL QSLVKTFSL KADNIVFKP 
      AIWTYIAII LLKILAVRD ENLRKLLDQ EASKSQLNT 
      WLSSLPNKT QIVSDTLNI LFDIDTFVE NYIYARGLI 
      FSVGFSRFK MNLNVNMFS AKKVKLHGM QSDKWTTKD 
      KINQYKGLI SLYTRDRKI IELDTNVTR RKLTRLIKD 
      LQRAVYNDR RELDNQIRG DKQKLRNTL AEHRELQLA 
      FQNSQPKKV IEEIKQRNF NRRKVLDRL KYEKKMRSQ 
      KLIDLKLEQ ALLEDRLKY AFLGQIKQE KEAQIITGK 
      LQDAILRKE GASAIYHIY REIIDIMKK DALYFDAII 
      ATVKYDGFS QSKCFLGAT KLGQLATEY LDDRRQEYD 
      TLEKIIIKD MTTRKTDIR VLQEHIDRA TASIKLLLR 
      KDSDLTTCA KSIRDSASM ENLRRDVGQ VEKTLVFLK 
      NTTLARSYD TIYPCLEEQ LKQKRRLKR LTKKCERDR 
      DIMLNKTNH ANLMNSVLK NTMNETTVE YRARKTELK 
      DEVERLRSE KKRYAKLLQ IKRTIAANI RIGLRQLLQ 
      VSYELSDET KKKPAPSSH PFGVLAERK DKPPEIIED 
      EIDGSKIVP ILVAKFTPL MSHYFGLKH EPKSDEAFR 
      NIESMMQER MILADQSEA PLSEASLLE GLYVEASEQ 
      LTREDIKKQ SQEIVAANT KNEDLVPLI VVPFNKWKK 
      MQKQQKN
    CDS sequence
    • >TC002966 RA
      atgcaagat tcgctcgaa gattatatt cgtatttcg 
      cgcaactgc aaagaggga ccaacccag gttaagaaa 
      gaagttctt attactgct caaataaaa aaggagtgt 
      gatgcgaaa gcactgcaa atacttcag tttgcaata 
      gaagggaaa atgaggtca gatgctttg ttcaaatgt 
      ttaccctac attaaccag tctcattac caagacatt 
      gtcgaggaa agagctatc agcaagctg tgcggatat 
      ccactttgt tccaaaaaa attccagac atgccaaag 
      aaacaattt tacatttcc actaaaaac aacaaggtt 
      tatgacata acagatcgc aagaattat tgtagcaat 
      ttttgctat cgcgccagt cttcatatc aaaggccaa 
      atagacacc agtccgtta tggcttaga aagtatcaa 
      gaaaaggcc cattacacg ctcctcaac aaatcagaa 
      aagggtctg ccaggagag atgatagat cagggtgta 
      ataaaacca gctgtggaa ccatatttt acttcagta 
      tccagtttt actcaaatg tcattagat gaggcagaa 
      gaattggag ttaaaccct caagaggag gagaaaata 
      aagcaaaaa aagaaaccc agaaagtac caaagaaat 
      gcaatgaaa accattaac gaaaatgag gaagaagaa 
      caagtcgaa tcaggagaa aaaatggac gaaatgaaa 
      gaatctgta gaaatacta aataaggaa aataaaaac 
      accccgaaa ataaaagac aaaccgtta gctcaaact 
      aaacaaaca aacgccact gacgtggaa tcacacatc 
      tacaaatgt attaaagag tggttaact ctagaaacg 
      ttcatgttt gttcacggc gaagaaaaa gtaaaagaa 
      aagctggat agtcaaaaa ctaagccac tactttgac 
      gctctgaaa atatcacaa ctgcaagtg gagcaacag 
      aaacgatac atcgacatt tgcagaaag ctgcattta 
      agagaattg gccgaagaa aaatttgac aaagccgtt 
      gtcggaaac caggccttg aaaccaatc cctgactat 
      cgacaacta aaggaagaa agccgcgaa atcgactta 
      aaggtgaag tgtttctac agtggcgaa atacatatc 
      caggaagac aaaaacttc ccctcgaaa aaggaagac 
      acgactaat gaagaagag gaacaaccg gcagtgctg 
      cccttggtc gatgtgaac tcacaaaac gcgttaaga 
      agaaaaatc ttcctaaat tcactcaac aaaacagta 
      caaacttta ttccaaact ttgaggata agtcccagc 
      ttggttttg tcagatttg caatccctg gttaagact 
      ttcagtctg aaagcggac aacattgtc ttcaaaccc 
      gcaatttgg acctacatt gctataatt ttgctgaaa 
      atcctggcc gtgagggac gaaaacttg cgcaagctt 
      ctggaccag gaggcgtcc aaaagtcag ttaaacacc 
      tggctgtct tcactcccc aacaaaacc caaatagtc 
      agtgatacc ttgaacatt ctttttgat atcgatacc 
      tttgtggaa aattatatt tatgcgcgt ggtttgatt 
      ttttctgtg ggtttttcg cgtttcaaa atgaattta 
      aacgttaac atgttttcg gccaaaaag gtgaagctc 
      catgggatg cagtccgac aaatggaca acaaaagac 
      aagataaat cagtacaag ggactcatc agtctttac 
      accagagac cggaagatt atcgaactc gacacaaat 
      gtgactcgg agaaaactg acgcggctt attaaagac 
      ttgcaacgg gcggtttac aatgatcga agggagctg 
      gacaaccaa attaggggc gataaacaa aaactgcgc 
      aacacttta gccgaacac cgggagctt cagttggcc 
      ttccaaaat tcacagccg aagaaagtg attgaggaa 
      attaaacaa cgtaatttt aacagacgc aaggtcctg 
      gaccggttg aaatacgag aaaaagatg cggtcgcaa 
      aaactgata gatctaaag ttggagcaa gcgctactg 
      gaggaccgc ctaaaatac gcattcctc ggccaaatc 
      aaacaggag aaagaggcg cagattata accggcaag 
      ttacaggac gctattttg cgcaaagaa ggcgcctcg 
      gcgatctat cacatctac cgcgagata atcgacatt 
      atgaaaaaa gacgcttta tactttgac gcgattata 
      gcaacggta aagtacgac ggcttcagc caaagcaaa 
      tgcttcctg ggggctacc aaactggga caactggcc 
      acggaatac ttggacgac aggagacaa gaatacgac 
      actttggaa aagattata atcaaagac atgaccact 
      cgcaagact gacattcgc gttttgcaa gaacacatt 
      gaccgcgcg actgccagc attaagctg ttgttgcga 
      aaagatagc gacctcaca acatgtgct aaaagtatt 
      agggattcc gccagcatg gaaaacctg cggcgggat 
      gtgggccag gttgaaaaa acccttgtg tttcttaaa 
      aacacaacg ttggcacgg tcttatgat acgatatat 
      ccttgcctg gaagaacag ctcaagcaa aaacggcgt 
      ctgaagcgc ctgactaaa aaatgtgaa cgcgataga 
      gacatcatg ttgaacaaa accaaccac gctaattta 
      atgaactcg gttttgaag aatacgatg aatgagaca 
      actgtcgaa tacagagca aggaagacg gaattaaag 
      gatgaagtg gagagactc aggagtgaa aaaaaaaga 
      tatgcaaaa ctcctacaa atcaagcga acaatagct 
      gcaaatatt cgcataggt ttgcggcaa cttttacaa 
      gtttcgtat gaactgtcg gatgaaact aagaaaaaa 
      cctgctcct agctcacac ccgtttggc gttttggcc 
      gagaggaag gacaaacca ccggaaatt atcgaagat 
      gaaatcgac ggatcaaaa attgtgcca attttggtg 
      gccaaattc accccctta atgtcacac tactttggc 
      ctcaaacac gagccaaag tccgatgag gcttttcga 
      aatattgaa tcaatgatg caggagcga atgattctg 
      gcagaccag tctgaggca cccctgagt gaagcttcc 
      ttgttggag ggtttatat gttgaagcc agtgagcaa 
      ctgacaagg gaggatatt aaaaaacag agtcaggaa 
      atcgttgca gcgaatacg aaaaacgaa gatttagtt 
      ccactaatt gtcgttccg ttcaacaag tggaagaaa 
      atgcaaaag cagcaaaaa aattaa
    mRNA sequence
    • >TC002966 RA
      taaaaagaa attgtaata aaataaatc acagtgagt 
      acctacata atatccaac aaatatata caaaaatta 
      tttcggaaa attaacaag ttctgtggc atcttcgct 
      gtcactaaa cctctatga caaacatgc gttaatact 
      tccaaacta aatgatggt ccaagagtc gcactattc 
      gaaaactgt agtcgagaa gaatcccac aacctaaaa 
      tcaagttca aaaaactca aaacaaatt aaaacttaa 
      tcccaagag aactaatat ttaaattaa acttaaatt 
      atggattaa ctgtcacca caactagta ccaaaacaa 
      ttgccaaca tttaacatt tattaaaaa atcgataaa 
      atgcacgaa tttaatcac gtgacatca atttccacc 
      aatcaacga gatgattga ggtcacgtg aatcgtgac 
      gttaaacag cctgtcaat ttgacaagt gtggaaggt 
      tatgctact tatgcactg ttcttcaat tttttttta 
      gtttttgat gaacagatt taatttctt taattaaaa 
      agtatgcaa gattcgctc gaagattat attcgtatt 
      tcgcgcaac tgcaaagag ggaccaacc caggttaag 
      aaagaagtt cttattact gctcaaata aaaaaggag 
      tgtgatgcg aaagcactg caaatactt cagtttgca 
      atagaaggg aaaatgagg tcagatgct ttgttcaaa 
      tgtttaccc tacattaac cagtctcat taccaagac 
      attgtcgag gaaagagct atcagcaag ctgtgcgga 
      tatccactt tgttccaaa aaaattcca gacatgcca 
      aagaaacaa ttttacatt tccactaaa aacaacaag 
      gtttatgac ataacagat cgcaagaat tattgtagc 
      aatttttgc tatcgcgcc agtcttcat atcaaaggc 
      caaatagac accagtccg ttatggctt agaaagtat 
      caagaaaag gcccattac acgctcctc aacaaatca 
      gaaaagggt ctgccagga gagatgata gatcagggt 
      gtaataaaa ccagctgtg gaaccatat tttacttca 
      gtatccagt tttactcaa atgtcatta gatgaggca 
      gaagaattg gagttaaac cctcaagag gaggagaaa 
      ataaagcaa aaaaagaaa cccagaaag taccaaaga 
      aatgcaatg aaaaccatt aacgaaaat gaggaagaa 
      gaacaagtc gaatcagga gaaaaaatg gacgaaatg 
      aaagaatct gtagaaata ctaaataag gaaaataaa 
      aacaccccg aaaataaaa gacaaaccg ttagctcaa 
      actaaacaa acaaacgcc actgacgtg gaatcacac 
      atctacaaa tgtattaaa gagtggtta actctagaa 
      acgttcatg tttgttcac ggcgaagaa aaagtaaaa 
      gaaaagctg gatagtcaa aaactaagc cactacttt 
      gacgctctg aaaatatca caactgcaa gtggagcaa 
      cagaaacga tacatcgac atttgcaga aagctgcat 
      ttaagagaa ttggccgaa gaaaaattt gacaaagcc 
      gttgtcgga aaccaggcc ttgaaacca atccctgac 
      tatcgacaa ctaaaggaa gaaagccgc gaaatcgac 
      ttaaaggtg aagtgtttc tacagtggc gaaatacat 
      atccaggaa gacaaaaac ttcccctcg aaaaaggaa 
      gacacgact aatgaagaa gaggaacaa ccggcagtg 
      ctgcccttg gtcgatgtg aactcacaa aacgcgtta 
      agaagaaaa atcttccta aattcactc aacaaaaca 
      gtacaaact ttattccaa actttgagg ataagtccc 
      agcttggtt ttgtcagat ttgcaatcc ctggttaag 
      actttcagt ctgaaagcg gacaacatt gtcttcaaa 
      cccgcaatt tggacctac attgctata attttgctg 
      aaaatcctg gccgtgagg gacgaaaac ttgcgcaag 
      cttctggac caggaggcg tccaaaagt cagttaaac 
      acctggctg tcttcactc cccaacaaa acccaaata 
      gtcagtgat accttgaac attcttttt gatatcgat 
      acctttgtg gaaaattat atttatgcg cgtggtttg 
      attttttct gtgggtttt tcgcgtttc aaaatgaat 
      ttaaacgtt aacatgttt tcggccaaa aaggtgaag 
      ctccatggg atgcagtcc gacaaatgg acaacaaaa 
      gacaagata aatcagtac aagggactc atcagtctt 
      tacaccaga gaccggaag attatcgaa ctcgacaca 
      aatgtgact cggagaaaa ctgacgcgg cttattaaa 
      gacttgcaa cgggcggtt tacaatgat cgaagggag 
      ctggacaac caaattagg ggcgataaa caaaaactg 
      cgcaacact ttagccgaa caccgggag cttcagttg 
      gccttccaa aattcacag ccgaagaaa gtgattgag 
      gaaattaaa caacgtaat tttaacaga cgcaaggtc 
      ctggaccgg ttgaaatac gagaaaaag atgcggtcg 
      caaaaactg atagatcta aagttggag caagcgcta 
      ctggaggac cgcctaaaa tacgcattc ctcggccaa 
      atcaaacag gagaaagag gcgcagatt ataaccggc 
      aagttacag gacgctatt ttgcgcaaa gaaggcgcc 
      tcggcgatc tatcacatc taccgcgag ataatcgac 
      attatgaaa aaagacgct ttatacttt gacgcgatt 
      atagcaacg gtaaagtac gacggcttc agccaaagc 
      aaatgcttc ctgggggct accaaactg ggacaactg 
      gccacggaa tacttggac gacaggaga caagaatac 
      gacactttg gaaaagatt ataatcaaa gacatgacc 
      actcgcaag actgacatt cgcgttttg caagaacac 
      attgaccgc gcgactgcc agcattaag ctgttgttg 
      cgaaaagat agcgacctc acaacatgt gctaaaagt 
      attagggat tccgccagc atggaaaac ctgcggcgg 
      gatgtgggc caggttgaa aaaaccctt gtgtttctt 
      aaaaacaca acgttggca cggtcttat gatacgata 
      tatccttgc ctggaagaa cagctcaag caaaaacgg 
      cgtctgaag cgcctgact aaaaaatgt gaacgcgat 
      agagacatc atgttgaac aaaaccaac cacgctaat 
      ttaatgaac tcggttttg aagaatacg atgaatgag 
      acaactgtc gaatacaga gcaaggaag acggaatta 
      aaggatgaa gtggagaga ctcaggagt gaaaaaaaa 
      agatatgca aaactccta caaatcaag cgaacaata 
      gctgcaaat attcgcata ggtttgcgg caactttta 
      caagtttcg tatgaactg tcggatgaa actaagaaa 
      aaacctgct cctagctca cacccgttt ggcgttttg 
      gccgagagg aaggacaaa ccaccggaa attatcgaa 
      gatgaaatc gacggatca aaaattgtg ccaattttg 
      gtggccaaa ttcaccccc ttaatgtca cactacttt 
      ggcctcaaa cacgagcca aagtccgat gaggctttt 
      cgaaatatt gaatcaatg atgcaggag cgaatgatt 
      ctggcagac cagtctgag gcacccctg agtgaagct 
      tccttgttg gagggttta tatgttgaa gccagtgag 
      caactgaca agggaggat attaaaaaa cagagtcag 
      gaaatcgtt gcagcgaat acgaaaaac gaagattta 
      gttccacta attgtcgtt ccgttcaac aagtggaag 
      aaaatgcaa aagcagcaa aaaaattaa tgggattta 
      atgataaat ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY