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Gene information
General information
TC002963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5706076..5709538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002963
    iBeetle screen iB_00483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002963 RA
      MTGRLPACV IDVGTGYTK LGFANNKEP QFIIPSAIA 
      IKETAKVGD QSSRRLCKG VEDLDFFIG DEAFDATGY 
      AVKYPVRHG LVEDWDLME RFLEQCIFK YLRAEPEDH 
      YFLLTEPPL NTPENREYT AEIMFESFN VPGLYIAVQ 
      AVLALAASW VGRSIEERT LTGIVIDSG DGVTHVIPV 
      AEGYVIGSC IKHIPIAGR NITYFIQSL LREREIGIP 
      PEQSLETAK AIKERHCYI CPDIAKEFA KYDADPAKW 
      MKKYEGMNS VTKQPFSVD VGYERFLGP EIFFHPEFS 
      NPDFTIPLS QIVDDVIQN CPIDVRRPM YNNIVLSGG 
      STMFKDFGR RLQRDIKKT VDARLKLSE TLGGGRLKP 
      KPIDVQVVS HHMQRYAVW FGGSMLAST PEFYTVCHT 
      KAAYEEYGP SICRHNPVF GTMT
    CDS sequence
    • >TC002963 RA
      atgactggg aggttacca gcttgtgtt atcgacgtc 
      ggcacaggt tacaccaaa ttaggcttc gccaataat 
      aaagaacca caatttata ataccctcc gctatagca 
      ataaaagaa accgccaaa gtcggcgat caatccagt 
      cgaagactg tgcaaaggt gttgaagat ttagatttc 
      tttataggc gatgaagcc ttcgacgct accggatat 
      gcagtcaaa tatcctgtt cgccatggg ttagtggag 
      gattgggat ttgatggaa aggtttcta gagcagtgt 
      atttttaaa tacctgcga gccgaaccg gaagatcac 
      tacttccta ttgactgaa ccaccattg aacaccccc 
      gaaaatagg gaatataca gccgaaatt atgtttgaa 
      tcgttcaat gtacctggc ttatacatt gctgtgcaa 
      gctgtctta gctttagcg gccagttgg gtcggccgc 
      agtatcgaa gaaagaact cttacaggc atcgtcatc 
      gacagtgga gacggtgtc acacacgtt attccagtg 
      gccgaaggc tatgttatc ggtagttgt atcaaacac 
      attcccatc gccggtcgc aacatcacc tacttcatc 
      cagtcgctt ctccgcgag cgcgaaatc ggcatcccc 
      cccgagcag tccctcgaa accgccaaa gcgatcaaa 
      gaacgccac tgctacatt tgccccgac atcgcaaag 
      gagtttgcg aaatacgac gcagacccg gccaaatgg 
      atgaaaaaa tacgagggg atgaactcg gttaccaaa 
      cagcctttc agcgtggac gtcggctac gagaggttt 
      ttgggacca gagattttc ttccatccc gaattttcc 
      aacccagac ttcaccatc ccgctgtcc cagatcgtg 
      gacgacgtg atccaaaac tgccccatt gatgtgcgg 
      aggcccatg tacaataat atcgttctg tcggggggc 
      tccaccatg ttcaaggat ttcgggcgc cggttgcag 
      agggacatc aagaagacg gtcgatgcg aggctcaag 
      cttagcgaa acccttggg ggtggcaga ttgaagcct 
      aaacctatt gatgttcaa gtagtgtct catcacatg 
      caacggtac gctgtttgg tttggaggg agtatgttg 
      gcgtcaacg cccgaattc tacaccgtt tgccacaca 
      aaggccgca tatgaagaa tatgggcct agcatttgc 
      aggcacaat cctgttttt ggaactatg acataa
    mRNA sequence
    • >TC002963 RA
      gggataggt cttgcgcag ttgtaccac ccgggaggt 
      gcggtttgt cctcccgat gagtgtgtt tggtttatg 
      atagtgatt gaaaaactg tcatttatc atatccaaa 
      ggtgtggtt tttatttca tttcctcgg ccaaatagt 
      aaattcctt tccaaattg tctgttatt ttttattaa 
      cacttaata gtcaatatg actgggagg ttaccagct 
      tgtgttatc gacgtcggc acaggttac accaaatta 
      ggcttcgcc aataataaa gaaccacaa tttataata 
      ccctccgct atagcaata aaagaaacc gccaaagtc 
      ggcgatcaa tccagtcga agactgtgc aaaggtgtt 
      gaagattta gatttcttt ataggcgat gaagccttc 
      gacgctacc ggatatgca gtcaaatat cctgttcgc 
      catgggtta gtggaggat tgggatttg atggaaagg 
      tttctagag cagtgtatt tttaaatac ctgcgagcc 
      gaaccggaa gatcactac ttcctattg actgaacca 
      ccattgaac acccccgaa aatagggaa tatacagcc 
      gaaattatg tttgaatcg ttcaatgta cctggctta 
      tacattgct gtgcaagct gtcttagct ttagcggcc 
      agttgggtc ggccgcagt atcgaagaa agaactctt 
      acaggcatc gtcatcgac agtggagac ggtgtcaca 
      cacgttatt ccagtggcc gaaggctat gttatcggt 
      agttgtatc aaacacatt cccatcgcc ggtcgcaac 
      atcacctac ttcatccag tcgcttctc cgcgagcgc 
      gaaatcggc atccccccc gagcagtcc ctcgaaacc 
      gccaaagcg atcaaagaa cgccactgc tacatttgc 
      cccgacatc gcaaaggag tttgcgaaa tacgacgca 
      gacccggcc aaatggatg aaaaaatac gaggggatg 
      aactcggtt accaaacag cctttcagc gtggacgtc 
      ggctacgag aggtttttg ggaccagag attttcttc 
      catcccgaa ttttccaac ccagacttc accatcccg 
      ctgtcccag atcgtggac gacgtgatc caaaactgc 
      cccattgat gtgcggagg cccatgtac aataatatc 
      gttctgtcg gggggctcc accatgttc aaggatttc 
      gggcgccgg ttgcagagg gacatcaag aagacggtc 
      gatgcgagg ctcaagctt agcgaaacc cttgggggt 
      ggcagattg aagcctaaa cctattgat gttcaagta 
      gtgtctcat cacatgcaa cggtacgct gtttggttt 
      ggagggagt atgttggcg tcaacgccc gaattctac 
      accgtttgc cacacaaag gccgcatat gaagaatat 
      gggcctagc atttgcagg cacaatcct gtttttgga 
      actatgaca taaataaat tacttgagt tgtctgtat 
      tattattgt gcattttta ttataaaat ttatagata 
      atattttaa tcgttttgt gaaatttaa ttttaatta 
      ataaaatat tagttctta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY