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Gene information
General information
TC002963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5706076..5709538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002963
    iBeetle screen iB_00483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002963 RA
      MTGRLPACV IDVGTGYTK LGFANNKEP QFIIPSAIA 
IKETAKVGD QSSRRLCKG VEDLDFFIG DEAFDATGY 
AVKYPVRHG LVEDWDLME RFLEQCIFK YLRAEPEDH 
YFLLTEPPL NTPENREYT AEIMFESFN VPGLYIAVQ 
AVLALAASW VGRSIEERT LTGIVIDSG DGVTHVIPV 
AEGYVIGSC IKHIPIAGR NITYFIQSL LREREIGIP 
PEQSLETAK AIKERHCYI CPDIAKEFA KYDADPAKW 
MKKYEGMNS VTKQPFSVD VGYERFLGP EIFFHPEFS 
NPDFTIPLS QIVDDVIQN CPIDVRRPM YNNIVLSGG 
STMFKDFGR RLQRDIKKT VDARLKLSE TLGGGRLKP 
KPIDVQVVS HHMQRYAVW FGGSMLAST PEFYTVCHT 
KAAYEEYGP SICRHNPVF GTMT
    CDS sequence
    • >TC002963 RA
      atgactggg aggttacca gcttgtgtt atcgacgtc 
ggcacaggt tacaccaaa ttaggcttc gccaataat 
aaagaacca caatttata ataccctcc gctatagca 
ataaaagaa accgccaaa gtcggcgat caatccagt 
cgaagactg tgcaaaggt gttgaagat ttagatttc 
tttataggc gatgaagcc ttcgacgct accggatat 
gcagtcaaa tatcctgtt cgccatggg ttagtggag 
gattgggat ttgatggaa aggtttcta gagcagtgt 
atttttaaa tacctgcga gccgaaccg gaagatcac 
tacttccta ttgactgaa ccaccattg aacaccccc 
gaaaatagg gaatataca gccgaaatt atgtttgaa 
tcgttcaat gtacctggc ttatacatt gctgtgcaa 
gctgtctta gctttagcg gccagttgg gtcggccgc 
agtatcgaa gaaagaact cttacaggc atcgtcatc 
gacagtgga gacggtgtc acacacgtt attccagtg 
gccgaaggc tatgttatc ggtagttgt atcaaacac 
attcccatc gccggtcgc aacatcacc tacttcatc 
cagtcgctt ctccgcgag cgcgaaatc ggcatcccc 
cccgagcag tccctcgaa accgccaaa gcgatcaaa 
gaacgccac tgctacatt tgccccgac atcgcaaag 
gagtttgcg aaatacgac gcagacccg gccaaatgg 
atgaaaaaa tacgagggg atgaactcg gttaccaaa 
cagcctttc agcgtggac gtcggctac gagaggttt 
ttgggacca gagattttc ttccatccc gaattttcc 
aacccagac ttcaccatc ccgctgtcc cagatcgtg 
gacgacgtg atccaaaac tgccccatt gatgtgcgg 
aggcccatg tacaataat atcgttctg tcggggggc 
tccaccatg ttcaaggat ttcgggcgc cggttgcag 
agggacatc aagaagacg gtcgatgcg aggctcaag 
cttagcgaa acccttggg ggtggcaga ttgaagcct 
aaacctatt gatgttcaa gtagtgtct catcacatg 
caacggtac gctgtttgg tttggaggg agtatgttg 
gcgtcaacg cccgaattc tacaccgtt tgccacaca 
aaggccgca tatgaagaa tatgggcct agcatttgc 
aggcacaat cctgttttt ggaactatg acataa
    mRNA sequence
    • >TC002963 RA
      gggataggt cttgcgcag ttgtaccac ccgggaggt 
gcggtttgt cctcccgat gagtgtgtt tggtttatg 
atagtgatt gaaaaactg tcatttatc atatccaaa 
ggtgtggtt tttatttca tttcctcgg ccaaatagt 
aaattcctt tccaaattg tctgttatt ttttattaa 
cacttaata gtcaatatg actgggagg ttaccagct 
tgtgttatc gacgtcggc acaggttac accaaatta 
ggcttcgcc aataataaa gaaccacaa tttataata 
ccctccgct atagcaata aaagaaacc gccaaagtc 
ggcgatcaa tccagtcga agactgtgc aaaggtgtt 
gaagattta gatttcttt ataggcgat gaagccttc 
gacgctacc ggatatgca gtcaaatat cctgttcgc 
catgggtta gtggaggat tgggatttg atggaaagg 
tttctagag cagtgtatt tttaaatac ctgcgagcc 
gaaccggaa gatcactac ttcctattg actgaacca 
ccattgaac acccccgaa aatagggaa tatacagcc 
gaaattatg tttgaatcg ttcaatgta cctggctta 
tacattgct gtgcaagct gtcttagct ttagcggcc 
agttgggtc ggccgcagt atcgaagaa agaactctt 
acaggcatc gtcatcgac agtggagac ggtgtcaca 
cacgttatt ccagtggcc gaaggctat gttatcggt 
agttgtatc aaacacatt cccatcgcc ggtcgcaac 
atcacctac ttcatccag tcgcttctc cgcgagcgc 
gaaatcggc atccccccc gagcagtcc ctcgaaacc 
gccaaagcg atcaaagaa cgccactgc tacatttgc 
cccgacatc gcaaaggag tttgcgaaa tacgacgca 
gacccggcc aaatggatg aaaaaatac gaggggatg 
aactcggtt accaaacag cctttcagc gtggacgtc 
ggctacgag aggtttttg ggaccagag attttcttc 
catcccgaa ttttccaac ccagacttc accatcccg 
ctgtcccag atcgtggac gacgtgatc caaaactgc 
cccattgat gtgcggagg cccatgtac aataatatc 
gttctgtcg gggggctcc accatgttc aaggatttc 
gggcgccgg ttgcagagg gacatcaag aagacggtc 
gatgcgagg ctcaagctt agcgaaacc cttgggggt 
ggcagattg aagcctaaa cctattgat gttcaagta 
gtgtctcat cacatgcaa cggtacgct gtttggttt 
ggagggagt atgttggcg tcaacgccc gaattctac 
accgtttgc cacacaaag gccgcatat gaagaatat 
gggcctagc atttgcagg cacaatcct gtttttgga 
actatgaca taaataaat tacttgagt tgtctgtat 
tattattgt gcattttta ttataaaat ttatagata 
atattttaa tcgttttgt gaaatttaa ttttaatta 
ataaaatat tagttctta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY