Status
Please wait ...
Gene information
General information
TC002960 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5777326..5782432
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002960
    iBeetle screen iB_00481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002960 RA
      MTSEQVQEL RNAFLQQLA SKGNDAVHP KDLERVKKD 
      DSWVNRFLM QHDNDQTEA LNMMWETLT WRKEFNVNE 
      INDHVKMDI IVQGGFFPH GHDKDGSTL FVFKCKKYV 
      KGTHNMDDL KRCVVYWFE RLERQDKGK PITLFFDME 
      GCGLANMDL EFTKYLIGL FKQYYPYFL NYILIFEMP 
      WILNAAFKI IKSWLPEKA VQKIKFVSK KDIKEYVPL 
      DQALKCWGG QNDYTFSFL PEPQEEVPN NVGTASSRK 
      VHFADGSSM SESPEGSGD RDHDGSALS VQPSSIITF 
      VKEGGELVS TVELQNTDS SLILSFKLK TTSPEKFRV 
      RPSVGCLTP GARATVHVT LLPGFQLGG LSRDKFLVM 
      STVIESSEM NQDLAQLWR DTTGRKVNQ HRLKCAQSN 
      DVSKNGNAV AVPGGSSDG DHTSSSQLM TKINNLTEC 
      QSQLHGAVK RIQYFQMVI VLLVIVVGV MLSYVLHLE 
      SQEGHTGSC SAP
    CDS sequence
    • >TC002960 RA
      atgacttcg gaacaagtt caggaactt agaaatgct 
      tttctccag cagcttgcg tcaaaaggg aacgatgcc 
      gttcatccc aaggacttg gaaagggtg aagaaggac 
      gatagctgg gtgaaccga ttcttaatg caacatgat 
      aatgaccaa actgaagcc ctaaacatg atgtgggag 
      acgctcact tggagaaaa gagtttaac gtcaatgag 
      attaatgac catgtaaag atggatatt atagtccag 
      ggcggattt tttccacat gggcatgat aaggatggt 
      agcaccttg tttgttttc aagtgtaag aaatacgtg 
      aaagggaca cacaacatg gatgatttg aaaagatgc 
      gtcgtatat tggtttgaa agattagaa cggcaagac 
      aaaggcaaa cctatcacc ctcttcttt gatatggaa 
      ggctgtggc ttagcaaat atggattta gaatttact 
      aaatatcta ataggcctc ttcaaacaa tattacccc 
      tatttttta aactacatt ctgattttt gaaatgcca 
      tggattttg aacgcggcc ttcaaaata ataaagtct 
      tggctaccc gagaaagcg gttcaaaaa atcaaattt 
      gtgagtaaa aaagacata aaagagtac gtccctctg 
      gatcaggca ctaaaatgt tggggtggg caaaacgac 
      tacaccttc agcttcctg ccagaaccc caagaggaa 
      gttccaaat aacgtcggc actgccagc agtaggaag 
      gttcatttt gctgatggg tcttcgatg tcagagtca 
      ccagaaggg agtggagac agggaccac gatggaagt 
      gcccttagt gtacaacca tccagcatc atcaccttc 
      gtcaaagag ggtggagag ctggttagt accgtggaa 
      ttgcaaaac actgatagt tctctcata ctctccttc 
      aagctaaaa accacatca ccggaaaaa ttccgcgtt 
      aggcccagt gtcgggtgt ttgacgccg ggagcacgc 
      gctacagtt catgtgact ctcttgccc ggttttcag 
      ctaggtggc ctgtccaga gacaaattc cttgtgatg 
      agcacagtc attgaatcc agtgaaatg aaccaggat 
      ctagcccag ctatggagg gatacaact ggcaggaag 
      gtcaaccag caccgtctg aagtgcgcc cagtcgaac 
      gacgtctcc aaaaacggc aacgccgtt gccgtgcct 
      gggggctcc tctgacggg gaccacacc agcagcagt 
      caacttatg acaaaaata aataactta acggagtgc 
      caaagtcag ctacatggg gctgtaaaa cgtattcag 
      tatttccag atggtaatc gttctcttg gttatcgtt 
      gtgggggtt atgttatct tatgtctta catttggaa 
      agtcaagag gggcacacc ggttcctgc agcgcccca 
      taa
    mRNA sequence
    • >TC002960 RA
      ggctgaact gcacatgtc ggatgattg cgattggtg 
      tatttattt tagtttatc aacacaagt gtcactact 
      tgtcaccag tcgtcatac atcactttt tatttcgtt 
      ggttgatag ttgtgattt agtttaagt tcaaggatc 
      gttaataac gtaagagtg gattaaagt gttatattg 
      tcaatgtct tatttaaat tccgtgtca ttaaaccca 
      gtgtaatct tattgaagt gttgaatta tttgttgtc 
      aaatcgaaa accaaagaa atgacttcg gaacaagtt 
      caggaactt agaaatgct tttctccag cagcttgcg 
      tcaaaaggg aacgatgcc gttcatccc aaggacttg 
      gaaagggtg aagaaggac gatagctgg gtgaaccga 
      ttcttaatg caacatgat aatgaccaa actgaagcc 
      ctaaacatg atgtgggag acgctcact tggagaaaa 
      gagtttaac gtcaatgag attaatgac catgtaaag 
      atggatatt atagtccag ggcggattt tttccacat 
      gggcatgat aaggatggt agcaccttg tttgttttc 
      aagtgtaag aaatacgtg aaagggaca cacaacatg 
      gatgatttg aaaagatgc gtcgtatat tggtttgaa 
      agattagaa cggcaagac aaaggcaaa cctatcacc 
      ctcttcttt gatatggaa ggctgtggc ttagcaaat 
      atggattta gaatttact aaatatcta ataggcctc 
      ttcaaacaa tattacccc tatttttta aactacatt 
      ctgattttt gaaatgcca tggattttg aacgcggcc 
      ttcaaaata ataaagtct tggctaccc gagaaagcg 
      gttcaaaaa atcaaattt gtgagtaaa aaagacata 
      aaagagtac gtccctctg gatcaggca ctaaaatgt 
      tggggtggg caaaacgac tacaccttc agcttcctg 
      ccagaaccc caagaggaa gttccaaat aacgtcggc 
      actgccagc agtaggaag gttcatttt gctgatggg 
      tcttcgatg tcagagtca ccagaaggg agtggagac 
      agggaccac gatggaagt gcccttagt gtacaacca 
      tccagcatc atcaccttc gtcaaagag ggtggagag 
      ctggttagt accgtggaa ttgcaaaac actgatagt 
      tctctcata ctctccttc aagctaaaa accacatca 
      ccggaaaaa ttccgcgtt aggcccagt gtcgggtgt 
      ttgacgccg ggagcacgc gctacagtt catgtgact 
      ctcttgccc ggttttcag ctaggtggc ctgtccaga 
      gacaaattc cttgtgatg agcacagtc attgaatcc 
      agtgaaatg aaccaggat ctagcccag ctatggagg 
      gatacaact ggcaggaag gtcaaccag caccgtctg 
      aagtgcgcc cagtcgaac gacgtctcc aaaaacggc 
      aacgccgtt gccgtgcct gggggctcc tctgacggg 
      gaccacacc agcagcagt caacttatg acaaaaata 
      aataactta acggagtgc caaagtcag ctacatggg 
      gctgtaaaa cgtattcag tatttccag atggtaatc 
      gttctcttg gttatcgtt gtgggggtt atgttatct 
      tatgtctta catttggaa agtcaagag gggcacacc 
      ggttcctgc agcgcccca taacaataa ctatcagac 
      gcaggcccg atcgaaata aatttggtt cgtaaactg 
      gtgcaaaat tcattgtac attgttttg tatttgatt 
      tgtatttta aaactggtt ttacgtttc taaccggtg 
      cctgcagtt catgacaat ataccacat tcctataaa 
      atttatttt tgttaaaag taaagtgct ttattttgg 
      gtattttaa tgtattaaa tcagtttga gtgatattg 
      ttaaaatgt gtgttgttg cgttggtat atgttacta 
      gcgccacaa cgtactgtt ttgtctagt gaatatatt 
      ttgtttaca cttttaaag gttatattg ttacaatat 
      ttaacttaa attagattt tatacctta gttaagata 
      ttttcatgc tatgtacaa tcgcattaa aagaactga 
      aaaaatgct aattgtata tattttgcc tatgtgtaa 
      gttacacaa ctatgtata gtgattttt atattgaag 
      gtcgtacat tctgtttaa cagtttgaa attatatct 
      ctttgtttg acatttaat aagagttta atcgatatt 
      atatttttt ttgaaaata ttttcttaa tgagacgta 
      ctgccacaa aaatttatt taagtagta ttaatatgt 
      atcaaatca accaatcaa aatttatat ttaggcaac 
      tcttttata taagtcaaa caaaaaccg aaaaatcag 
      tcaagtgct acaaattac caaatacac tcattgtta 
      aagcctacg ctacgaata ttgccttac ctaattgta 
      caacaaaaa aggacaaac tgtggtttt ctaaagcta 
      gttaggcag ctgatctcc aactcattt tttactctt 
      atagcgaat caaatgtct aaaaacagt tgcctaagc 
      taaaatttt gaagcatgt agttaaaaa aggctagaa 
      acgtaaatg tagtaatta tttttaatc cacaaaatt 
      cgtttgttc ataagtgat gaataaatt gatgaaaaa 
      act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY