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Gene information
General information
TC002954 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5835512..5836902
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002954
    iBeetle screen iB_00478

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002954 RA
      MYFLIFLLL ASISVSRAE IDIQSQDFI DSINQKQSH 
      WVARRNFPE NTTNEYLYK LNGFLGLHP DPNYMPEKI 
      KHNFNPQDI PKTFDARKK WPKCDSLNR IRDQGSCGS 
      CWAFAAVET MSDRICIHS SGAKKFFFS AEDLLSCCT 
      ACGSCSGGY MMAAFDFYI KQGVVSGGD LNSNEGCRP 
      YTADAHDKG VTPSCTKSC RKGYPTSYS SDKHYGSKD 
      YIVDAGVSN IQYEIMTNG PIIVSFKVY QDFYNYGSG 
      VYHHVSGNY TGNHIVKIV GWGTEKEQD YWLIANSWG 
      SSWGEHGFF KILRGKNEC GIENNPYAV LPKL
    CDS sequence
    • >TC002954 RA
      atgtacttt ttgattttc ttgttgctt gcctcaatt 
      tctgtttca agagcagaa attgacatc caatcacaa 
      gattttatc gattcgatc aaccaaaag caatcgcat 
      tgggtcgct cgtcgaaat ttccccgaa aacaccact 
      aatgagtat ctctacaaa ctgaacggg tttctcgga 
      cttcatccc gatcccaat tacatgccc gaaaaaatc 
      aagcacaat ttcaaccct caagatatc ccaaagact 
      ttcgatgcc aggaaaaag tggccaaag tgtgactcc 
      ctgaacaga attcgggat caagggtct tgtgggtct 
      tgctgggca tttgcagcg gtggagacg atgtctgac 
      cgaatatgt attcattcg tcaggagca aagaaattt 
      ttcttttcg gcggaagat cttctttct tgttgtaca 
      gcttgtggg agctgtagc ggaggatac atgatggct 
      gcgttcgac ttttatatt aaacaagga gtcgtttct 
      ggtggagat ttaaactca aatgaaggt tgcaggccg 
      tatactgca gatgctcac gacaaaggc gtgactcct 
      tcatgcacc aaaagttgt cgcaaaggc tatccaact 
      agttacagt tcagataag cactatggc agcaaagat 
      tacattgtt gatgctgga gttagcaac attcagtac 
      gagattatg accaatgga cctattatt gtaagtttt 
      aaagtgtac caagatttt tataattac gggtctgga 
      gtttaccac cacgtgagt ggaaattat accggtaat 
      catattgtt aaaatagtg ggatggggc actgaaaaa 
      gaacaagac tactggctc attgctaat tcctggggc 
      agtagttgg ggtgaacat ggatttttc aaaatatta 
      agaggcaaa aatgaatgt gggattgaa aacaatccg 
      tatgcagta ttgcctaaa ctttga
    mRNA sequence
    • >TC002954 RA
      attattccc aatttagag aataattgg gccatcaaa 
      ttacctaat gaaaatact gcggtaaaa tgggtcaaa 
      atcacgcaa tgttttaaa gataaaatt tccgtttca 
      caactgtta tcattagat ttaatgata atcctttaa 
      ttaaatctt tgattaaag gacgtgata gtaaaatca 
      aagctatat aaattgagc gttttattt tttttcggt 
      cataacaaa gttttcgaa aatgtactt tttgatttt 
      cttgttgct tgcctcaat ttctgtttc aagagcaga 
      aattgacat ccaatcaca agattttat cgattcgat 
      caaccaaaa gcaatcgca ttgggtcgc tcgtcgaaa 
      tttccccga aaacaccac taatgagta tctctacaa 
      actgaacgg gtttctcgg acttcatcc cgatcccaa 
      ttacatgcc cgaaaaaat caagcacaa tttcaaccc 
      tcaagatat cccaaagac tttcgatgc caggaaaaa 
      gtggccaaa gtgtgactc cctgaacag aattcggga 
      tcaagggtc ttgtgggtc ttgctgggc atttgcagc 
      ggtggagac gatgtctga ccgaatatg tattcattc 
      gtcaggagc aaagaaatt tttcttttc ggcggaaga 
      tcttctttc ttgttgtac agcttgtgg gagctgtag 
      cggaggata catgatggc tgcgttcga cttttatat 
      taaacaagg agtcgtttc tggtggaga tttaaactc 
      aaatgaagg ttgcaggcc gtatactgc agatgctca 
      cgacaaagg cgtgactcc ttcatgcac caaaagttg 
      tcgcaaagg ctatccaac tagttacag ttcagataa 
      gcactatgg cagcaaaga ttacattgt tgatgctgg 
      agttagcaa cattcagta cgagattat gaccaatgg 
      acctattat tgtaagttt taaagtgta ccaagattt 
      ttataatta cgggtctgg agtttacca ccacgtgag 
      tggaaatta taccggtaa tcatattgt taaaatagt 
      gggatgggg cactgaaaa agaacaaga ctactggct 
      cattgctaa ttcctgggg cagtagttg gggtgaaca 
      tggattttt caaaatatt aagaggcaa aaatgaatg 
      tgggattga aaacaatcc gtatgcagt attgcctaa 
      actttgaac atattttat ttagtgcaa ttctgattt 
      ttaaataaa gatgtcgtt gaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY