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Gene information
General information
TC034585 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5990314..5998587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034585
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    potentially confused/crosscontaminated with : adults of..., iB # (comma separated)
    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) (egglay)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034585 RA
      MYGSFNELY SGREIWNSN NWRNRNSRS GVRRNRSPS 
      TSSERSFPG RNRSGFVPG QQDQRGGRR PNQNARKMG 
      FKALQDLLN KDSEDLILE LSNIKRGIQ DYIKTSLSD 
      DGIVLFVKV LKKVSDSAF VENKYKVLQ LYLDEVFVD 
      KLVKYVALL PMQSDLDRT RNQFFWENT DEFWNNVIG 
      ICQDIYNAI PTFGFKVLP NILKSLLVC FPAFQDQHK 
      QHISDGIRD SVNKLNESL QIMIAEEEE KKKASIQKK 
      VSTLIEEEP PDDFHQISV YPNSLELLG GTRVFLRKN 
      IVEGPYKDV HHYLDVQFR LLREDFVGP LRNGIATYR 
      TESTQKQRI ENVNIYKKV QFLNQETIN EQYCIRLRF 
      DFSRKTKKF RFEDSKKFM FGSLLCFTC DNFKSLLFG 
      KIAQRDVKD LEKGELIVG FDQNVLVDY KANYLMIEC 
      GIYFEPYFH VLNVLKNIN TDEFPMEKY IIRVDPTPQ 
      PPEYLNDPT PRKISIRGH DFFPLLNWP NTNFYTFNQ 
      SQLRAFRAA LTQEFSVIQ GPPGTGKTF LGLKIAHTL 
      LQNQAIWFK KTPMLVICY TNHALDQFL EGLALATDR 
      IIRIGGQSR NQNVAKFNL RNIKFRTNP AVLQQRHGV 
      KVLLLQIQN ITENLKEID FYHSIRDFK VFIDVIPNF 
      ITTWFHRAT IDELLDWLL PEYENEDLD STNEVKSPR 
      SVAEVQDKV ANLQIEDEM FDENLSDYE IEEDENMKI 
      ELDDLFDEV NAMAVQCSI PLITLSSLQ QRISALERT 
      FQQLKQKEE ITMEDIFEE DRIVFELYK LKFRYDSLN 
      FRLTEGKQM RFMKRRVPD WSEFTNPHK LSPDDRWNL 
      YFFCLNLYR QKLNEKLSV LNEQFREQH KIYEEMRDI 
      ENTKAMKNV LVVGMTTTG AARLRSSLQ TLKSPIVIV 
      EEAAEILEA HIVSSLTKH CKHLILIGD HQQLKPSTA 
      SYNIEKFYN LGISLFERM VVNRIQLNT LNVQHRMRP 
      EIASLVSPT IYPTLQDHP SVNDRPDIK GVDNCLFFI 
      DHKHPEANC EGKSKKNYH EVDFLIYFA RHLILNGYE 
      PGNITILAA YLGQMFELQ KERRKHNEL LANVRIAVL 
      DNYQGEECD IILLSLVRN NEENKIGFL SIENRVCVA 
      LSRARNGFY LMGNMDQLC AASQLWREI YKTFERQNA 
      IGPHLALRC QVHPDKVTY VASGKDFLN ISEGGCNQK 
      CGADLNCGH QCTSLCHVL NRDHASYRC LETCGKKLC 
      DKNELHLCQ RRCYQECGP CTYKVSRTL KCGHVVNLE 
      CHLDPATYS CEELVPTQL PCGHNADKP CHCNPETFP 
      CPFPCEKQV EPCGHACTR NCHVRQDPD HLEYKCKKR 
      CAKNQKNCK AEPEKHKCK KLCFEDCDA CMVRVRKQR 
      TICPHFYDV PCSSNVDDI SCEKPCTKI LPCNHKCKN 
      KCNQPCGNC KEKVEKQIP VCAHTIKVK CSEEPLRKF 
      CTKKCPLTL PCGHPCTKK CNESCTPTC TVLATCALK 
      APCGHTVTK LPCHLAHGA LDPKTLLQH CNHPCGGQL 
      LCEHTCSGT CGECSQGRI HKRCDAKCG NPLVCNHIC 
      EFPCRQACR PCVRPCPYK CKHSACGKR CGDICVPCK 
      ESCPRRCKH RRCSKYCGE ICDVPPCQE PCSKLLACG 
      HQCIGFCGD PCPQKCRIC DEDEVTEVF FGFEAEDDA 
      RFVLLEDCG HIFENTGLE TWLGQDDGE VIKFKECPR 
      CKTAIKTTA RFSDYVKKA KADVAKVKK NLHGTENEL 
      NMKRQELAR LLLKVRTNA QDVKCSNFF KDILGAMET 
      RLRISLMGK RQRINKFEL NAIESKLQI IEHIVDIGI 
      NCPTALSKN RCSSQVKFI LKILEREDN YVTDQEIDD 
      IPYELNRLS RIAELEEIE SSPNFLQRY SNSSTVRRV 
      TEQIEGFLF GFGKFYDQD EQTVKELLQ ELSRLVASG 
      IGISEQEKR EIVKAMGFR QGHWFKCPN GHPYCIADC 
      GGAMVESKC PECACKIGG GSHTLRADN ALASEMDGA 
      QFPAWSETA NNMGNFLLD FDD
    CDS sequence
    • >TC034585 RA
      atgtacgga agttttaac gaattatat tcaggaaga 
      gaaatttgg aattccaac aactggaga aaccgtaat 
      tctaggagc ggagtacga cgaaaccga agtccaagt 
      accagttcc gagcggtcc tttcctggt aggaatcgt 
      tctggattc gtcccagga caacaagac caaagaggt 
      ggcagaaga cctaaccaa aatgcaaga aaaatgggt 
      ttcaaagcc ctgcaagat ttgttaaac aaggattcc 
      gaggattta atcctagaa ttgtccaac attaaaaga 
      ggcatacag gactacatc aaaacgagt ttgtccgac 
      gatggcatt gtgctgttc gtcaaagtt ttgaagaaa 
      gtgtcggac tcggccttt gttgaaaac aagtacaag 
      gttttacaa ttatatctg gatgaagta tttgttgac 
      aaattggta aagtatgtt gctttattg ccaatgcaa 
      agcgattta gaccgaaca aggaatcaa tttttttgg 
      gaaaacacc gatgagttt tggaacaat gttatagga 
      atttgccag gatatctat aatgcaatc cccacattt 
      gggttcaaa gttttacca aatatactg aaaagtttg 
      ctcgtctgt ttccccgcg tttcaagac caacacaag 
      caacatatt tcggatggg ataagggac agtgtgaac 
      aaactcaat gaaagtttg caaatcatg attgcagag 
      gaagaagaa aagaagaaa gcttctata caaaagaaa 
      gtttcaaca ttaattgag gaagaacca ccggatgat 
      tttcatcaa atttctgtc taccccaat tcacttgaa 
      ctccttgga ggcactcga gtttttctg cgtaaaaac 
      atagtagaa ggaccttat aaagacgta caccactac 
      ttggacgtc cagtttaga ctgctaaga gaagacttt 
      gttgggcct ttgagaaat gggatagcc acgtaccga 
      acagaatca acccagaag cagcgaatt gaaaatgtc 
      aatatttac aaaaaagta cagttttta aatcaagag 
      acaatcaac gaacaatac tgcattcga ttaaggttc 
      gacttttca cgcaaaaca aagaaattt cgctttgaa 
      gactccaag aaattcatg tttggttct ctcctctgc 
      ttcacctgc gacaacttc aagtcacta ctctttggc 
      aaaattgct cagcgagac gtgaaagac cttgaaaag 
      ggggaactg attgtaggt tttgatcaa aatgtgctc 
      gtggactac aaagcgaac tatttgatg atcgaatgc 
      ggaatctac ttcgagccg tatttccac gtcctcaac 
      gtcctcaaa aacataaac actgacgaa ttcccgatg 
      gaaaaatac atcattcgc gtggacccc acacctcaa 
      ccgccggaa tacctcaac gacccaacc ccccgtaaa 
      atctcaatc cgtggtcat gattttttc cctctcctc 
      aactggccc aacaccaac ttctacact ttcaaccaa 
      tcgcaacta agggccttc cgggcagct ttaacgcaa 
      gagttctcc gttattcaa ggcccacct ggcactggg 
      aagaccttc cttggcctc aaaatcgcc cacactttg 
      ctgcaaaat caggccatt tggttcaag aaaaccccg 
      atgttggtc atctgctac accaaccat gccctcgac 
      cagttcttg gagggtttg gctctggcc actgacaga 
      atcattcga attggggga cagagcaga aaccaaaat 
      gtggcaaag ttcaatttg agaaatata aagttccgg 
      acaaacccg gccgtttta caacaaaga cacggagtg 
      aaagtcctc ttgctacaa atccagaat ataacggaa 
      aacttgaaa gaaatcgat ttttatcac agcatcaga 
      gatttcaaa gtttttatt gatgtgata ccgaatttt 
      attacaaca tggtttcat cgcgctaca atagacgaa 
      ctactggac tggttgctg cccgaatat gaaaatgaa 
      gacttggat tcgactaat gaagttaaa agtcctagg 
      agtgttgca gaggtccag gacaaagtc gctaacttg 
      cagattgaa gatgaaatg tttgatgaa aatttgtcg 
      gattatgaa atcgaagaa gatgaaaat atgaaaatt 
      gagttggat gatcttttc gatgaagtg aatgcaatg 
      gcggttcag tgcagcatt ccccttatt acgctctcc 
      tcacttcag caaaggatt tctgcacta gaaagaact 
      ttccagcaa ctgaagcaa aaggaagag ataactatg 
      gaggatatt ttcgaagaa gatcgaatt gtcttcgaa 
      ttgtataaa ctgaaattt cgttacgac agtttaaat 
      tttagacta accgagggc aagcaaatg cgtttcatg 
      aaaagaaga gttcctgat tggtcagag ttcacgaat 
      cctcacaaa ttgtcaccc gacgatcgc tggaattta 
      tactttttt tgtttaaat ttatacaga cagaaatta 
      aatgagaag ctgagcgta ctaaatgag cagttccgc 
      gaacaacac aaaatctat gaagaaatg cgggacatt 
      gaaaacacg aaagcaatg aaaaacgtg cttgttgtt 
      ggtatgacg accactgga gcagcccgt ctaagatct 
      tcgttgcaa acgttgaaa agtccaatc gttattgtc 
      gaggaagca gcggagatt ctagaagct cacatcgtt 
      tcctctttg accaaacat tgcaaacac ttgattctc 
      atcggagac catcagcag ttgaaaccg agcactgcg 
      agttataac atagaaaag ttttacaat ttggggata 
      agtctattt gagagaatg gttgtgaac agaatccag 
      ttgaatacc ctaaacgtg caacacaga atgaggccg 
      gagattgcc agtttggtc tcgccgacc atttatcca 
      accctgcaa gatcacccg tctgtgaac gacagacct 
      gatataaaa ggggtcgat aattgcttg tttttcatc 
      gaccacaag caccctgaa gcaaattgt gaaggcaaa 
      agcaagaag aattaccat gaagttgac ttcttgatc 
      tatttcgca aggcatctt attctgaac ggttacgaa 
      cccggaaac atcacaatc ctggcggct tacttgggg 
      caaatgttc gagttgcag aaagagagg cgcaaacac 
      aatgagctt ctagcgaac gtaagaatt gcagttttg 
      gataactat caaggtgaa gaatgcgat attatccta 
      ctttcgctg gttcgaaac aacgaggaa aacaaaatc 
      gggttccta tcaattgaa aacagagtt tgtgtggcg 
      ctttcaaga gccagaaac ggcttctat ctcatgggg 
      aacatggac cagttgtgt gccgctagt caactttgg 
      cgtgagatt tacaagact tttgaacgc cagaatgcc 
      attggaccc cacctagcc ctacggtgc caagtgcac 
      ccagataag gtcacgtat gtggcaagc ggaaaggat 
      tttctgaat atatcagaa ggagggtgc aaccagaaa 
      tgtggggct gatttgaat tgcggtcat cagtgcact 
      tctctttgc cacgtgtta aatcgagat catgcaagc 
      tacaggtgc cttgaaacg tgcgggaaa aaattgtgc 
      gataaaaat gaactacat ttatgccaa agaaggtgt 
      taccaagag tgtggtcct tgcacttac aaggtgtca 
      aggacccta aaatgtggg catgttgtg aacttggag 
      tgtcacctg gatcctgca acttacagt tgtgaggag 
      ctcgtgcct acgcaactt ccctgtggt cacaacgct 
      gacaagcct tgccactgc aatccggaa acttttcct 
      tgcccgttc ccctgcgaa aaacaggtg gaaccttgt 
      ggccatgcg tgcactaga aattgccac gttcggcag 
      gatccagat cacttagaa tacaaatgt aagaaacgt 
      tgcgctaaa aaccaaaag aattgcaaa gccgaaccc 
      gaaaaacac aagtgcaag aaactatgt tttgaggat 
      tgtgacgcc tgtatggtc cgtgttagg aaacagcga 
      accatctgt ccacatttt tacgacgtg ccttgttcc 
      agtaacgtg gacgatata agctgcgaa aagccttgc 
      accaaaata ttgccttgc aatcacaaa tgcaaaaat 
      aaatgcaat caaccgtgc ggaaactgc aaagaaaag 
      gttgagaaa caaatcccg gtatgtgcc catacaatc 
      aaagtaaaa tgtagcgaa gaacctctc cgaaaattc 
      tgtaccaaa aaatgtcct ctgacacta ccctgtggc 
      cacccttgc acgaaaaaa tgcaacgag tcttgcacc 
      ccaacttgc accgttctg gccacctgt gcattgaaa 
      gccccttgt ggccacacc gtgaccaag ttaccgtgc 
      cacctagcc catggtgcc ctcgaccct aaaaccctg 
      ctacaacac tgcaaccac ccttgcggt ggccagtta 
      ctgtgcgag cacacctgc tcgggaacc tgcggggag 
      tgctcgcag ggccgcatc cacaaacgc tgcgacgca 
      aaatgcggg aaccccctg gtgtgcaac cacatctgc 
      gagttcccc tgcagacag gcctgcagg ccctgcgtg 
      cgcccttgc ccctacaag tgcaagcac agcgcctgt 
      gggaagcgc tgcggcgat atctgcgtc ccttgcaag 
      gagagctgc ccccgcagg tgcaagcac cgaaggtgc 
      tccaagtac tgcggggaa atctgcgac gtgccccct 
      tgtcaagag ccttgcagc aaattactg gcatgtggc 
      caccaatgt atcgggttc tgcggcgat ccttgccct 
      caaaaatgc cgcatttgt gacgaagac gaggtgacc 
      gaagtgttt ttcgggttc gaggctgaa gacgatgcc 
      cggtttgtg ctcctggag gactgcggc cacattttc 
      gaaaatacg ggactggaa acgtggctg gggcaagac 
      gacggggaa gtgatcaag ttcaaggag tgtccgcgg 
      tgcaaaacg gcgatcaaa acaacggcc cgattcagt 
      gattatgtg aagaaagcg aaggccgat gtggccaaa 
      gttaagaaa aatctgcac gggactgag aatgagttg 
      aacatgaag aggcaagaa ctggcgaga ctgctacta 
      aaagttagg acgaatgca caagatgtg aaatgttcc 
      aacttcttt aaagatatt ttgggcgcg atggaaacc 
      cgtctgcga atttctcta atgggcaag cgccaacgt 
      atcaacaaa tttgagttg aatgcaatt gaatccaaa 
      ctccaaatc atcgaacac atcgttgac attggcata 
      aactgcccc acggctctt tcaaaaaat cgctgctcc 
      tctcaggtc aaattcata ctcaagatt ttggaacgt 
      gaggacaac tacgtaacg gatcaagaa atagacgat 
      atcccgtac gaattgaac cgtttgtca cgaatcgcc 
      gaacttgaa gaaatcgaa agttcgcca aacttcctc 
      caacgatat tcaaattct tccacagtt aggcgcgtt 
      accgaacaa attgaaggc tttctcttc ggtttcggc 
      aagttttat gaccaggat gaacaaacg gtcaaggaa 
      ttattgcaa gagttgagt cgattggtc gcgtcggga 
      ataggaatt tcagaacag gaaaaaagg gaaattgtt 
      aaagccatg ggtttcagg caagggcat tggttcaag 
      tgccccaat ggccaccct tactgcata gctgactgt 
      gggggcgca atggtggag tcaaaatgc ccggaatgt 
      gcgtgcaaa ataggggga ggtagtcac acactgcgc 
      gccgataat gcgttggct tctgaaatg gatggagcc 
      cagtttccc gcttggtcg gaaactgca aacaatatg 
      ggcaatttt ttgttagat ttcgatgat tga
    mRNA sequence
    • >TC034585 RA
      cgcggacgc ggtggtgga aaccctaac tggcggtcc 
      cgtcgagtt gataatgaa atgtacgga agttttaac 
      gaattatat tcaggaaga gaaatttgg aattccaac 
      aactggaga aaccgtaat tctaggagc ggagtacga 
      cgaaaccga agtccaagt accagttcc gagcggtcc 
      tttcctggt aggaatcgt tctggattc gtcccagga 
      caacaagac caaagaggt ggcagaaga cctaaccaa 
      aatgcaaga aaaatgggt ttcaaagcc ctgcaagat 
      ttgttaaac aaggattcc gaggattta atcctagaa 
      ttgtccaac attaaaaga ggcatacag gactacatc 
      aaaacgagt ttgtccgac gatggcatt gtgctgttc 
      gtcaaagtt ttgaagaaa gtgtcggac tcggccttt 
      gttgaaaac aagtacaag gttttacaa ttatatctg 
      gatgaagta tttgttgac aaattggta aagtatgtt 
      gctttattg ccaatgcaa agcgattta gaccgaaca 
      aggaatcaa tttttttgg gaaaacacc gatgagttt 
      tggaacaat gttatagga atttgccag gatatctat 
      aatgcaatc cccacattt gggttcaaa gttttacca 
      aatatactg aaaagtttg ctcgtctgt ttccccgcg 
      tttcaagac caacacaag caacatatt tcggatggg 
      ataagggac agtgtgaac aaactcaat gaaagtttg 
      caaatcatg attgcagag gaagaagaa aagaagaaa 
      gcttctata caaaagaaa gtttcaaca ttaattgag 
      gaagaacca ccggatgat tttcatcaa atttctgtc 
      taccccaat tcacttgaa ctccttgga ggcactcga 
      gtttttctg cgtaaaaac atagtagaa ggaccttat 
      aaagacgta caccactac ttggacgtc cagtttaga 
      ctgctaaga gaagacttt gttgggcct ttgagaaat 
      gggatagcc acgtaccga acagaatca acccagaag 
      cagcgaatt gaaaatgtc aatatttac aaaaaagta 
      cagttttta aatcaagag acaatcaac gaacaatac 
      tgcattcga ttaaggttc gacttttca cgcaaaaca 
      aagaaattt cgctttgaa gactccaag aaattcatg 
      tttggttct ctcctctgc ttcacctgc gacaacttc 
      aagtcacta ctctttggc aaaattgct cagcgagac 
      gtgaaagac cttgaaaag ggggaactg attgtaggt 
      tttgatcaa aatgtgctc gtggactac aaagcgaac 
      tatttgatg atcgaatgc ggaatctac ttcgagccg 
      tatttccac gtcctcaac gtcctcaaa aacataaac 
      actgacgaa ttcccgatg gaaaaatac atcattcgc 
      gtggacccc acacctcaa ccgccggaa tacctcaac 
      gacccaacc ccccgtaaa atctcaatc cgtggtcat 
      gattttttc cctctcctc aactggccc aacaccaac 
      ttctacact ttcaaccaa tcgcaacta agggccttc 
      cgggcagct ttaacgcaa gagttctcc gttattcaa 
      ggcccacct ggcactggg aagaccttc cttggcctc 
      aaaatcgcc cacactttg ctgcaaaat caggccatt 
      tggttcaag aaaaccccg atgttggtc atctgctac 
      accaaccat gccctcgac cagttcttg gagggtttg 
      gctctggcc actgacaga atcattcga attggggga 
      cagagcaga aaccaaaat gtggcaaag ttcaatttg 
      agaaatata aagttccgg acaaacccg gccgtttta 
      caacaaaga cacggagtg aaagtcctc ttgctacaa 
      atccagaat ataacggaa aacttgaaa gaaatcgat 
      ttttatcac agcatcaga gatttcaaa gtttttatt 
      gatgtgata ccgaatttt attacaaca tggtttcat 
      cgcgctaca atagacgaa ctactggac tggttgctg 
      cccgaatat gaaaatgaa gacttggat tcgactaat 
      gaagttaaa agtcctagg agtgttgca gaggtccag 
      gacaaagtc gctaacttg cagattgaa gatgaaatg 
      tttgatgaa aatttgtcg gattatgaa atcgaagaa 
      gatgaaaat atgaaaatt gagttggat gatcttttc 
      gatgaagtg aatgcaatg gcggttcag tgcagcatt 
      ccccttatt acgctctcc tcacttcag caaaggatt 
      tctgcacta gaaagaact ttccagcaa ctgaagcaa 
      aaggaagag ataactatg gaggatatt ttcgaagaa 
      gatcgaatt gtcttcgaa ttgtataaa ctgaaattt 
      cgttacgac agtttaaat tttagacta accgagggc 
      aagcaaatg cgtttcatg aaaagaaga gttcctgat 
      tggtcagag ttcacgaat cctcacaaa ttgtcaccc 
      gacgatcgc tggaattta tactttttt tgtttaaat 
      ttatacaga cagaaatta aatgagaag ctgagcgta 
      ctaaatgag cagttccgc gaacaacac aaaatctat 
      gaagaaatg cgggacatt gaaaacacg aaagcaatg 
      aaaaacgtg cttgttgtt ggtatgacg accactgga 
      gcagcccgt ctaagatct tcgttgcaa acgttgaaa 
      agtccaatc gttattgtc gaggaagca gcggagatt 
      ctagaagct cacatcgtt tcctctttg accaaacat 
      tgcaaacac ttgattctc atcggagac catcagcag 
      ttgaaaccg agcactgcg agttataac atagaaaag 
      ttttacaat ttggggata agtctattt gagagaatg 
      gttgtgaac agaatccag ttgaatacc ctaaacgtg 
      caacacaga atgaggccg gagattgcc agtttggtc 
      tcgccgacc atttatcca accctgcaa gatcacccg 
      tctgtgaac gacagacct gatataaaa ggggtcgat 
      aattgcttg tttttcatc gaccacaag caccctgaa 
      gcaaattgt gaaggcaaa agcaagaag aattaccat 
      gaagttgac ttcttgatc tatttcgca aggcatctt 
      attctgaac ggttacgaa cccggaaac atcacaatc 
      ctggcggct tacttgggg caaatgttc gagttgcag 
      aaagagagg cgcaaacac aatgagctt ctagcgaac 
      gtaagaatt gcagttttg gataactat caaggtgaa 
      gaatgcgat attatccta ctttcgctg gttcgaaac 
      aacgaggaa aacaaaatc gggttccta tcaattgaa 
      aacagagtt tgtgtggcg ctttcaaga gccagaaac 
      ggcttctat ctcatgggg aacatggac cagttgtgt 
      gccgctagt caactttgg cgtgagatt tacaagact 
      tttgaacgc cagaatgcc attggaccc cacctagcc 
      ctacggtgc caagtgcac ccagataag gtcacgtat 
      gtggcaagc ggaaaggat tttctgaat atatcagaa 
      ggagggtgc aaccagaaa tgtggggct gatttgaat 
      tgcggtcat cagtgcact tctctttgc cacgtgtta 
      aatcgagat catgcaagc tacaggtgc cttgaaacg 
      tgcgggaaa aaattgtgc gataaaaat gaactacat 
      ttatgccaa agaaggtgt taccaagag tgtggtcct 
      tgcacttac aaggtgtca aggacccta aaatgtggg 
      catgttgtg aacttggag tgtcacctg gatcctgca 
      acttacagt tgtgaggag ctcgtgcct acgcaactt 
      ccctgtggt cacaacgct gacaagcct tgccactgc 
      aatccggaa acttttcct tgcccgttc ccctgcgaa 
      aaacaggtg gaaccttgt ggccatgcg tgcactaga 
      aattgccac gttcggcag gatccagat cacttagaa 
      tacaaatgt aagaaacgt tgcgctaaa aaccaaaag 
      aattgcaaa gccgaaccc gaaaaacac aagtgcaag 
      aaactatgt tttgaggat tgtgacgcc tgtatggtc 
      cgtgttagg aaacagcga accatctgt ccacatttt 
      tacgacgtg ccttgttcc agtaacgtg gacgatata 
      agctgcgaa aagccttgc accaaaata ttgccttgc 
      aatcacaaa tgcaaaaat aaatgcaat caaccgtgc 
      ggaaactgc aaagaaaag gttgagaaa caaatcccg 
      gtatgtgcc catacaatc aaagtaaaa tgtagcgaa 
      gaacctctc cgaaaattc tgtaccaaa aaatgtcct 
      ctgacacta ccctgtggc cacccttgc acgaaaaaa 
      tgcaacgag tcttgcacc ccaacttgc accgttctg 
      gccacctgt gcattgaaa gccccttgt ggccacacc 
      gtgaccaag ttaccgtgc cacctagcc catggtgcc 
      ctcgaccct aaaaccctg ctacaacac tgcaaccac 
      ccttgcggt ggccagtta ctgtgcgag cacacctgc 
      tcgggaacc tgcggggag tgctcgcag ggccgcatc 
      cacaaacgc tgcgacgca aaatgcggg aaccccctg 
      gtgtgcaac cacatctgc gagttcccc tgcagacag 
      gcctgcagg ccctgcgtg cgcccttgc ccctacaag 
      tgcaagcac agcgcctgt gggaagcgc tgcggcgat 
      atctgcgtc ccttgcaag gagagctgc ccccgcagg 
      tgcaagcac cgaaggtgc tccaagtac tgcggggaa 
      atctgcgac gtgccccct tgtcaagag ccttgcagc 
      aaattactg gcatgtggc caccaatgt atcgggttc 
      tgcggcgat ccttgccct caaaaatgc cgcatttgt 
      gacgaagac gaggtgacc gaagtgttt ttcgggttc 
      gaggctgaa gacgatgcc cggtttgtg ctcctggag 
      gactgcggc cacattttc gaaaatacg ggactggaa 
      acgtggctg gggcaagac gacggggaa gtgatcaag 
      ttcaaggag tgtccgcgg tgcaaaacg gcgatcaaa 
      acaacggcc cgattcagt gattatgtg aagaaagcg 
      aaggccgat gtggccaaa gttaagaaa aatctgcac 
      gggactgag aatgagttg aacatgaag aggcaagaa 
      ctggcgaga ctgctacta aaagttagg acgaatgca 
      caagatgtg aaatgttcc aacttcttt aaagatatt 
      ttgggcgcg atggaaacc cgtctgcga atttctcta 
      atgggcaag cgccaacgt atcaacaaa tttgagttg 
      aatgcaatt gaatccaaa ctccaaatc atcgaacac 
      atcgttgac attggcata aactgcccc acggctctt 
      tcaaaaaat cgctgctcc tctcaggtc aaattcata 
      ctcaagatt ttggaacgt gaggacaac tacgtaacg 
      gatcaagaa atagacgat atcccgtac gaattgaac 
      cgtttgtca cgaatcgcc gaacttgaa gaaatcgaa 
      agttcgcca aacttcctc caacgatat tcaaattct 
      tccacagtt aggcgcgtt accgaacaa attgaaggc 
      tttctcttc ggtttcggc aagttttat gaccaggat 
      gaacaaacg gtcaaggaa ttattgcaa gagttgagt 
      cgattggtc gcgtcggga ataggaatt tcagaacag 
      gaaaaaagg gaaattgtt aaagccatg ggtttcagg 
      caagggcat tggttcaag tgccccaat ggccaccct 
      tactgcata gctgactgt gggggcgca atggtggag 
      tcaaaatgc ccggaatgt gcgtgcaaa ataggggga 
      ggtagtcac acactgcgc gccgataat gcgttggct 
      tctgaaatg gatggagcc cagtttccc gcttggtcg 
      gaaactgca aacaatatg ggcaatttt ttgttagat 
      ttcgatgat tgatccagt taattaaac acgattata 
      cttt
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