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Gene information
General information
TC002936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1479278..1484931
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002936
    iBeetle screen iB_00473
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002936 RA
      MTTQQNQPK RGIVKQILS GDSVIIRGP TGAPPPEKQ 
      INFSGIVAP KLARRAGDQ SEPTKDEPW AWEAREFLR 
      KKLIGEEVF FTSEKPPNA NREYGTVYL GKDFNSAEN 
      ITESLVSEG LVTVRREGV RQSPEGARL AELEDAAKA 
      AGKGKWGSS PPSEHVRDI KWSVENMRS FVDKLGYKP 
      VKAIIEHVR DGSTVRAFL LPEFYHVTL MISGIRCPG 
      FKLDANGKP DPSIKVPYA EEARYFVEI RLLQREVDI 
      VLESVNNNN FVGTIIHPK GNIAEALLK EGFAHCVDW 
      SIAFMKSGV EGLRAAEKK AKMARLRIW KDWQSNAPQ 
      VTGKEKEFS ATVAEVING DALSVKLNN GQYKKIFLS 
      SIRPPKEPG RVADEDGKT APRPKGFRP LYDIPWMFE 
      AREYLRKKL IGKKVHVVI DYIQEARDG YPEKVCATV 
      TVGGKNVAE ALVAKGLAS VVKYRPDDD QRSSKYDDL 
      LAAESKAMK SGIGIHNKK DVPIHRVTE IDAARAKLE 
      LSSFQRAQR IDAVVEFVA SGTRLRVFI PKSNSLCTF 
      LLGGINCPR ASRQATNAQ PAVEGEPFG DEALQFTKE 
      KCLQREVSI QVDTHDKAG NFIGWLWID NVNLSVALV 
      KEGFASVHR TGEKSQYAA LLKEAEDSA KQHRLRIWK 
      NYEEEKEEP HAEEEKPNV ERKVSYEEV VVTEVTPEG 
      SFFVQTISE GPKAEALNA KLRQEFQAN PPLPGAYTP 
      KRGDICAAK YTVDDEWYR VKVEKVQGG KASVHYIDY 
      GNRETLPST RLASLPAAY AGEKPYATE YILPYVTLP 
      KDEEYAAMA LKYLREDTA VSKLLLNVE YRVQGGPSA 
      ASLHTDNTA EGDIIKNLI TEGLLLVEN RKERRQNKL 
      LGAYKEAQE VAKRNHSNI WEYGDITED DAKEFGLGK
    CDS sequence
    • >TC002936 RA
      atgactacc caacaaaat cagccaaaa cgtggaatt 
      gtgaaacaa atcctctct ggggattct gtaataatc 
      cggggcccc accggagca cctcccccc gagaaacaa 
      atcaatttt tcggggatc gtcgccccc aagttggcc 
      cgcagagcc ggagaccag tccgaaccg acgaaagac 
      gagccatgg gcttgggaa gctcgcgag ttcctccgc 
      aaaaagctc atcggagag gaggttttc ttcacctca 
      gagaagccc ccgaatgcc aatcgggag tacggcacg 
      gtgtatttg ggcaaagac ttcaactcg gccgaaaac 
      atcaccgag tcgttggtc tcggagggc cttgtgacg 
      gtgcgtcgc gagggcgtc cggcagagc cctgagggg 
      gcccgtctg gccgagctc gaggacgcc gccaaggcc 
      gccggtaag ggtaaatgg ggcagcagc cccccttca 
      gagcacgtc cgagacatc aaatggtcg gttgagaac 
      atgcgctct ttcgtcgat aaattgggg tacaagccc 
      gtgaaggcc atcattgaa cacgtccgc gatggctcc 
      actgtccgt gcgttcctc ctccctgag ttctaccac 
      gtcacgctt atgatttcg ggaattagg tgtcccggt 
      tttaaactg gacgctaat ggtaagccc gaccccagc 
      attaaggtt ccatatgct gaagaggct cgctatttc 
      gttgaaatt cgtctcttg caaagagaa gttgatatt 
      gttttggaa tcggtcaat aataataat tttgtgggt 
      actattatc catccgaaa ggcaacatt gctgaagct 
      ctgttgaaa gagggcttc gcccattgc gttgattgg 
      tcgattgcg tttatgaag tcgggggtt gaaggtttg 
      agagctgct gagaaaaaa gcgaaaatg gcccggctt 
      cggatttgg aaagactgg caatcgaat gcaccgcag 
      gttacgggc aaagaaaag gagttttcg gctacagtt 
      gctgaagtt attaatgga gatgcactc tctgttaag 
      ttgaacaat ggccaatat aagaagatc tttttgtcg 
      agtataaga ccaccgaaa gagccaggc cgtgttgcc 
      gatgaagac ggcaagact gccccgaga cctaaagga 
      ttccgtcca ctgtacgat attccatgg atgtttgaa 
      gcaagagag tacttacgc aagaaactc atcggtaag 
      aaagtccac gtcgtcatt gactacatc caagaagcc 
      agagacggt taccctgaa aaagtttgc gctactgtt 
      acagttggt ggaaaaaat gttgccgaa gcacttgtt 
      gcaaaaggt ctcgcgtcc gttgtgaaa taccgccct 
      gatgacgat caacgtagc tccaaatac gatgatctt 
      ttggctgcc gaaagcaaa gccatgaaa tcaggaatc 
      gggattcat aacaagaag gacgttccg attcaccga 
      gtgactgaa attgatgct gccagagcc aaactcgaa 
      ttgtcttcc ttccaacgt gcacaaaga atcgacgcc 
      gttgttgaa tttgtcgcc agtggaacc agattaaga 
      gttttcata ccgaaatcg aacagttta tgtaccttt 
      ttgttagga ggtataaat tgtccgcgc gcttcccgc 
      caagcaacc aatgcccag cccgccgtc gaaggcgaa 
      cctttcggt gatgaagca ttgcaattt acaaaggaa 
      aagtgtctt cagagagaa gtttcgatt caagttgat 
      actcacgac aaagccggc aatttcatt ggctggctc 
      tggattgac aatgttaac ttatcggtc gctctcgtt 
      aaagaaggt ttcgcttcc gtgcaccgc accggagaa 
      aaatcacaa tatgctgcc ctcctcaaa gaagccgaa 
      gacagtgcc aaacaacac cgtttacga atatggaag 
      aactacgaa gaggagaaa gaggaacca cacgcagaa 
      gaggagaaa cccaacgtt gagcgaaaa gtgagttac 
      gaggaagtg gtggtgaca gaagtcacc cccgagggt 
      tcgttcttc gtccaaacc atctccgag ggtcccaaa 
      gccgaagcg ttgaacgcc aaacttcgc caagaattc 
      caagccaat ccgcccctt cccggcgcc tacactcca 
      aagcgtggc gacatctgt gctgccaaa tacaccgtt 
      gacgacgag tggtacagg gttaaggtc gagaaggtg 
      caagggggc aaggcctcc gttcactac atcgactac 
      ggcaaccgc gaaacgctt ccaagcacc cgcctcgcc 
      agtctaccc gccgcctac gcaggggag aagccctac 
      gccaccgaa tacatcctg ccttacgtc accctgccc 
      aaagacgag gaatacgct gcgatggcc ctcaagtat 
      ctgcgagag gacacggcg gtcagcaaa ctgctgctg 
      aacgtcgag tatcgcgtg caagggggc ccatctgcc 
      gcttcgctc catactgat aatactgca gaaggggat 
      attatcaag aatttgatc acggagggg ttgctcctg 
      gtcgagaac agaaaggag agacggcag aataaattg 
      cttggcgcg tacaaggag gcccaggaa gtggccaag 
      aggaaccat tcgaacatt tgggagtat ggtgacatc 
      actgaagat gatgccaag gagtttgga ctggggaaa 
      tga
    mRNA sequence
    • >TC002936 RA
      taccaaata aaatgataa atgggtggt acttacagt 
      taggtttgc ggtcacgtt gagtcccaa attactgta 
      caattttca agtggttcc aaagaaggg aatcaattt 
      ttaacccac cccttgacg ctttcaccg agtcacgtg 
      actgagtga cgttcatcc acctttcag tacaagttg 
      catggcccg ttcacgatg actacccaa caaaatcag 
      ccaaaacgt ggaattgtg aaacaaatc ctctctggg 
      gattctgta ataatccgg ggccccacc ggagcacct 
      ccccccgag aaacaaatc aatttttcg gggatcgtc 
      gcccccaag ttggcccgc agagccgga gaccagtcc 
      gaaccgacg aaagacgag ccatgggct tgggaagct 
      cgcgagttc ctccgcaaa aagctcatc ggagaggag 
      gttttcttc acctcagag aagcccccg aatgccaat 
      cgggagtac ggcacggtg tatttgggc aaagacttc 
      aactcggcc gaaaacatc accgagtcg ttggtctcg 
      gagggcctt gtgacggtg cgtcgcgag ggcgtccgg 
      cagagccct gagggggcc cgtctggcc gagctcgag 
      gacgccgcc aaggccgcc ggtaagggt aaatggggc 
      agcagcccc ccttcagag cacgtccga gacatcaaa 
      tggtcggtt gagaacatg cgctctttc gtcgataaa 
      ttggggtac aagcccgtg aaggccatc attgaacac 
      gtccgcgat ggctccact gtccgtgcg ttcctcctc 
      cctgagttc taccacgtc acgcttatg atttcggga 
      attaggtgt cccggtttt aaactggac gctaatggt 
      aagcccgac cccagcatt aaggttcca tatgctgaa 
      gaggctcgc tatttcgtt gaaattcgt ctcttgcaa 
      agagaagtt gatattgtt ttggaatcg gtcaataat 
      aataatttt gtgggtact attatccat ccgaaaggc 
      aacattgct gaagctctg ttgaaagag ggcttcgcc 
      cattgcgtt gattggtcg attgcgttt atgaagtcg 
      ggggttgaa ggtttgaga gctgctgag aaaaaagcg 
      aaaatggcc cggcttcgg atttggaaa gactggcaa 
      tcgaatgca ccgcaggtt acgggcaaa gaaaaggag 
      ttttcggct acagttgct gaagttatt aatggagat 
      gcactctct gttaagttg aacaatggc caatataag 
      aagatcttt ttgtcgagt ataagacca ccgaaagag 
      ccaggccgt gttgccgat gaagacggc aagactgcc 
      ccgagacct aaaggattc cgtccactg tacgatatt 
      ccatggatg tttgaagca agagagtac ttacgcaag 
      aaactcatc ggtaagaaa gtccacgtc gtcattgac 
      tacatccaa gaagccaga gacggttac cctgaaaaa 
      gtttgcgct actgttaca gttggtgga aaaaatgtt 
      gccgaagca cttgttgca aaaggtctc gcgtccgtt 
      gtgaaatac cgccctgat gacgatcaa cgtagctcc 
      aaatacgat gatcttttg gctgccgaa agcaaagcc 
      atgaaatca ggaatcggg attcataac aagaaggac 
      gttccgatt caccgagtg actgaaatt gatgctgcc 
      agagccaaa ctcgaattg tcttccttc caacgtgca 
      caaagaatc gacgccgtt gttgaattt gtcgccagt 
      ggaaccaga ttaagagtt ttcataccg aaatcgaac 
      agtttatgt acctttttg ttaggaggt ataaattgt 
      ccgcgcgct tcccgccaa gcaaccaat gcccagccc 
      gccgtcgaa ggcgaacct ttcggtgat gaagcattg 
      caatttaca aaggaaaag tgtcttcag agagaagtt 
      tcgattcaa gttgatact cacgacaaa gccggcaat 
      ttcattggc tggctctgg attgacaat gttaactta 
      tcggtcgct ctcgttaaa gaaggtttc gcttccgtg 
      caccgcacc ggagaaaaa tcacaatat gctgccctc 
      ctcaaagaa gccgaagac agtgccaaa caacaccgt 
      ttacgaata tggaagaac tacgaagag gagaaagag 
      gaaccacac gcagaagag gagaaaccc aacgttgag 
      cgaaaagtg agttacgag gaagtggtg gtgacagaa 
      gtcaccccc gagggttcg ttcttcgtc caaaccatc 
      tccgagggt cccaaagcc gaagcgttg aacgccaaa 
      cttcgccaa gaattccaa gccaatccg ccccttccc 
      ggcgcctac actccaaag cgtggcgac atctgtgct 
      gccaaatac accgttgac gacgagtgg tacagggtt 
      aaggtcgag aaggtgcaa gggggcaag gcctccgtt 
      cactacatc gactacggc aaccgcgaa acgcttcca 
      agcacccgc ctcgccagt ctacccgcc gcctacgca 
      ggggagaag ccctacgcc accgaatac atcctgcct 
      tacgtcacc ctgcccaaa gacgaggaa tacgctgcg 
      atggccctc aagtatctg cgagaggac acggcggtc 
      agcaaactg ctgctgaac gtcgagtat cgcgtgcaa 
      gggggccca tctgccgct tcgctccat actgataat 
      actgcagaa ggggatatt atcaagaat ttgatcacg 
      gaggggttg ctcctggtc gagaacaga aaggagaga 
      cggcagaat aaattgctt ggcgcgtac aaggaggcc 
      caggaagtg gccaagagg aaccattcg aacatttgg 
      gagtatggt gacatcact gaagatgat gccaaggag 
      tttggactg gggaaatga gggaccatt cattcagag 
      cgacattaa cctacatta aattttaaa actagctgt 
      gggttgcaa aacttctct agtagactt tccatttaa 
      ctaggatag atgccaata taggatata tttttatcc 
      agcaccatt attatacag atgttgatg tgtatgttt 
      attttatat ggtttgtac aatatggaa attatgaat 
      ggaatattg tggccttta cataatgtt ttacatttt 
      tattaacac ttttatgtt taaattcgt tcacttgac 
      gcattcaat tttactttt tatgtatat ttctggttg 
      tgacaattt atttttaca cgatttcct gtatatttt 
      ggctaatcg aacagctac ctttcgtat tatgtatat 
      tttgtgccc tatgttaat cgtatctct tattcaaat 
      ttatgttat ttataagtt atcaggtgt aatatcatt 
      acttcaaat gatttgatt aataagtat aatccaact 
      tgattcatt caagttttg agtgcatct ggtcatgac 
      acacagact gggtataat tttatgtac gtactgtaa 
      aacaatttt tacttcgat tgaaggcgt ggatgtaac 
      taatgtagg gagtggctc tgtgtaaat taaataaaa 
      gcattattg ttat