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Gene information
General information
TC002936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6040565..6046218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002936
    iBeetle screen iB_00473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002936 RA
      MTTQQNQPK RGIVKQILS GDSVIIRGP TGAPPPEKQ 
INFSGIVAP KLARRAGDQ SEPTKDEPW AWEAREFLR 
KKLIGEEVF FTSEKPPNA NREYGTVYL GKDFNSAEN 
ITESLVSEG LVTVRREGV RQSPEGARL AELEDAAKA 
AGKGKWGSS PPSEHVRDI KWSVENMRS FVDKLGYKP 
VKAIIEHVR DGSTVRAFL LPEFYHVTL MISGIRCPG 
FKLDANGKP DPSIKVPYA EEARYFVEI RLLQREVDI 
VLESVNNNN FVGTIIHPK GNIAEALLK EGFAHCVDW 
SIAFMKSGV EGLRAAEKK AKMARLRIW KDWQSNAPQ 
VTGKEKEFS ATVAEVING DALSVKLNN GQYKKIFLS 
SIRPPKEPG RVADEDGKT APRPKGFRP LYDIPWMFE 
AREYLRKKL IGKKVHVVI DYIQEARDG YPEKVCATV 
TVGGKNVAE ALVAKGLAS VVKYRPDDD QRSSKYDDL 
LAAESKAMK SGIGIHNKK DVPIHRVTE IDAARAKLE 
LSSFQRAQR IDAVVEFVA SGTRLRVFI PKSNSLCTF 
LLGGINCPR ASRQATNAQ PAVEGEPFG DEALQFTKE 
KCLQREVSI QVDTHDKAG NFIGWLWID NVNLSVALV 
KEGFASVHR TGEKSQYAA LLKEAEDSA KQHRLRIWK 
NYEEEKEEP HAEEEKPNV ERKVSYEEV VVTEVTPEG 
SFFVQTISE GPKAEALNA KLRQEFQAN PPLPGAYTP 
KRGDICAAK YTVDDEWYR VKVEKVQGG KASVHYIDY 
GNRETLPST RLASLPAAY AGEKPYATE YILPYVTLP 
KDEEYAAMA LKYLREDTA VSKLLLNVE YRVQGGPSA 
ASLHTDNTA EGDIIKNLI TEGLLLVEN RKERRQNKL 
LGAYKEAQE VAKRNHSNI WEYGDITED DAKEFGLGK
    CDS sequence
    • >TC002936 RA
      atgactacc caacaaaat cagccaaaa cgtggaatt 
gtgaaacaa atcctctct ggggattct gtaataatc 
cggggcccc accggagca cctcccccc gagaaacaa 
atcaatttt tcggggatc gtcgccccc aagttggcc 
cgcagagcc ggagaccag tccgaaccg acgaaagac 
gagccatgg gcttgggaa gctcgcgag ttcctccgc 
aaaaagctc atcggagag gaggttttc ttcacctca 
gagaagccc ccgaatgcc aatcgggag tacggcacg 
gtgtatttg ggcaaagac ttcaactcg gccgaaaac 
atcaccgag tcgttggtc tcggagggc cttgtgacg 
gtgcgtcgc gagggcgtc cggcagagc cctgagggg 
gcccgtctg gccgagctc gaggacgcc gccaaggcc 
gccggtaag ggtaaatgg ggcagcagc cccccttca 
gagcacgtc cgagacatc aaatggtcg gttgagaac 
atgcgctct ttcgtcgat aaattgggg tacaagccc 
gtgaaggcc atcattgaa cacgtccgc gatggctcc 
actgtccgt gcgttcctc ctccctgag ttctaccac 
gtcacgctt atgatttcg ggaattagg tgtcccggt 
tttaaactg gacgctaat ggtaagccc gaccccagc 
attaaggtt ccatatgct gaagaggct cgctatttc 
gttgaaatt cgtctcttg caaagagaa gttgatatt 
gttttggaa tcggtcaat aataataat tttgtgggt 
actattatc catccgaaa ggcaacatt gctgaagct 
ctgttgaaa gagggcttc gcccattgc gttgattgg 
tcgattgcg tttatgaag tcgggggtt gaaggtttg 
agagctgct gagaaaaaa gcgaaaatg gcccggctt 
cggatttgg aaagactgg caatcgaat gcaccgcag 
gttacgggc aaagaaaag gagttttcg gctacagtt 
gctgaagtt attaatgga gatgcactc tctgttaag 
ttgaacaat ggccaatat aagaagatc tttttgtcg 
agtataaga ccaccgaaa gagccaggc cgtgttgcc 
gatgaagac ggcaagact gccccgaga cctaaagga 
ttccgtcca ctgtacgat attccatgg atgtttgaa 
gcaagagag tacttacgc aagaaactc atcggtaag 
aaagtccac gtcgtcatt gactacatc caagaagcc 
agagacggt taccctgaa aaagtttgc gctactgtt 
acagttggt ggaaaaaat gttgccgaa gcacttgtt 
gcaaaaggt ctcgcgtcc gttgtgaaa taccgccct 
gatgacgat caacgtagc tccaaatac gatgatctt 
ttggctgcc gaaagcaaa gccatgaaa tcaggaatc 
gggattcat aacaagaag gacgttccg attcaccga 
gtgactgaa attgatgct gccagagcc aaactcgaa 
ttgtcttcc ttccaacgt gcacaaaga atcgacgcc 
gttgttgaa tttgtcgcc agtggaacc agattaaga 
gttttcata ccgaaatcg aacagttta tgtaccttt 
ttgttagga ggtataaat tgtccgcgc gcttcccgc 
caagcaacc aatgcccag cccgccgtc gaaggcgaa 
cctttcggt gatgaagca ttgcaattt acaaaggaa 
aagtgtctt cagagagaa gtttcgatt caagttgat 
actcacgac aaagccggc aatttcatt ggctggctc 
tggattgac aatgttaac ttatcggtc gctctcgtt 
aaagaaggt ttcgcttcc gtgcaccgc accggagaa 
aaatcacaa tatgctgcc ctcctcaaa gaagccgaa 
gacagtgcc aaacaacac cgtttacga atatggaag 
aactacgaa gaggagaaa gaggaacca cacgcagaa 
gaggagaaa cccaacgtt gagcgaaaa gtgagttac 
gaggaagtg gtggtgaca gaagtcacc cccgagggt 
tcgttcttc gtccaaacc atctccgag ggtcccaaa 
gccgaagcg ttgaacgcc aaacttcgc caagaattc 
caagccaat ccgcccctt cccggcgcc tacactcca 
aagcgtggc gacatctgt gctgccaaa tacaccgtt 
gacgacgag tggtacagg gttaaggtc gagaaggtg 
caagggggc aaggcctcc gttcactac atcgactac 
ggcaaccgc gaaacgctt ccaagcacc cgcctcgcc 
agtctaccc gccgcctac gcaggggag aagccctac 
gccaccgaa tacatcctg ccttacgtc accctgccc 
aaagacgag gaatacgct gcgatggcc ctcaagtat 
ctgcgagag gacacggcg gtcagcaaa ctgctgctg 
aacgtcgag tatcgcgtg caagggggc ccatctgcc 
gcttcgctc catactgat aatactgca gaaggggat 
attatcaag aatttgatc acggagggg ttgctcctg 
gtcgagaac agaaaggag agacggcag aataaattg 
cttggcgcg tacaaggag gcccaggaa gtggccaag 
aggaaccat tcgaacatt tgggagtat ggtgacatc 
actgaagat gatgccaag gagtttgga ctggggaaa 
tga
    mRNA sequence
    • >TC002936 RA
      taccaaata aaatgataa atgggtggt acttacagt 
taggtttgc ggtcacgtt gagtcccaa attactgta 
caattttca agtggttcc aaagaaggg aatcaattt 
ttaacccac cccttgacg ctttcaccg agtcacgtg 
actgagtga cgttcatcc acctttcag tacaagttg 
catggcccg ttcacgatg actacccaa caaaatcag 
ccaaaacgt ggaattgtg aaacaaatc ctctctggg 
gattctgta ataatccgg ggccccacc ggagcacct 
ccccccgag aaacaaatc aatttttcg gggatcgtc 
gcccccaag ttggcccgc agagccgga gaccagtcc 
gaaccgacg aaagacgag ccatgggct tgggaagct 
cgcgagttc ctccgcaaa aagctcatc ggagaggag 
gttttcttc acctcagag aagcccccg aatgccaat 
cgggagtac ggcacggtg tatttgggc aaagacttc 
aactcggcc gaaaacatc accgagtcg ttggtctcg 
gagggcctt gtgacggtg cgtcgcgag ggcgtccgg 
cagagccct gagggggcc cgtctggcc gagctcgag 
gacgccgcc aaggccgcc ggtaagggt aaatggggc 
agcagcccc ccttcagag cacgtccga gacatcaaa 
tggtcggtt gagaacatg cgctctttc gtcgataaa 
ttggggtac aagcccgtg aaggccatc attgaacac 
gtccgcgat ggctccact gtccgtgcg ttcctcctc 
cctgagttc taccacgtc acgcttatg atttcggga 
attaggtgt cccggtttt aaactggac gctaatggt 
aagcccgac cccagcatt aaggttcca tatgctgaa 
gaggctcgc tatttcgtt gaaattcgt ctcttgcaa 
agagaagtt gatattgtt ttggaatcg gtcaataat 
aataatttt gtgggtact attatccat ccgaaaggc 
aacattgct gaagctctg ttgaaagag ggcttcgcc 
cattgcgtt gattggtcg attgcgttt atgaagtcg 
ggggttgaa ggtttgaga gctgctgag aaaaaagcg 
aaaatggcc cggcttcgg atttggaaa gactggcaa 
tcgaatgca ccgcaggtt acgggcaaa gaaaaggag 
ttttcggct acagttgct gaagttatt aatggagat 
gcactctct gttaagttg aacaatggc caatataag 
aagatcttt ttgtcgagt ataagacca ccgaaagag 
ccaggccgt gttgccgat gaagacggc aagactgcc 
ccgagacct aaaggattc cgtccactg tacgatatt 
ccatggatg tttgaagca agagagtac ttacgcaag 
aaactcatc ggtaagaaa gtccacgtc gtcattgac 
tacatccaa gaagccaga gacggttac cctgaaaaa 
gtttgcgct actgttaca gttggtgga aaaaatgtt 
gccgaagca cttgttgca aaaggtctc gcgtccgtt 
gtgaaatac cgccctgat gacgatcaa cgtagctcc 
aaatacgat gatcttttg gctgccgaa agcaaagcc 
atgaaatca ggaatcggg attcataac aagaaggac 
gttccgatt caccgagtg actgaaatt gatgctgcc 
agagccaaa ctcgaattg tcttccttc caacgtgca 
caaagaatc gacgccgtt gttgaattt gtcgccagt 
ggaaccaga ttaagagtt ttcataccg aaatcgaac 
agtttatgt acctttttg ttaggaggt ataaattgt 
ccgcgcgct tcccgccaa gcaaccaat gcccagccc 
gccgtcgaa ggcgaacct ttcggtgat gaagcattg 
caatttaca aaggaaaag tgtcttcag agagaagtt 
tcgattcaa gttgatact cacgacaaa gccggcaat 
ttcattggc tggctctgg attgacaat gttaactta 
tcggtcgct ctcgttaaa gaaggtttc gcttccgtg 
caccgcacc ggagaaaaa tcacaatat gctgccctc 
ctcaaagaa gccgaagac agtgccaaa caacaccgt 
ttacgaata tggaagaac tacgaagag gagaaagag 
gaaccacac gcagaagag gagaaaccc aacgttgag 
cgaaaagtg agttacgag gaagtggtg gtgacagaa 
gtcaccccc gagggttcg ttcttcgtc caaaccatc 
tccgagggt cccaaagcc gaagcgttg aacgccaaa 
cttcgccaa gaattccaa gccaatccg ccccttccc 
ggcgcctac actccaaag cgtggcgac atctgtgct 
gccaaatac accgttgac gacgagtgg tacagggtt 
aaggtcgag aaggtgcaa gggggcaag gcctccgtt 
cactacatc gactacggc aaccgcgaa acgcttcca 
agcacccgc ctcgccagt ctacccgcc gcctacgca 
ggggagaag ccctacgcc accgaatac atcctgcct 
tacgtcacc ctgcccaaa gacgaggaa tacgctgcg 
atggccctc aagtatctg cgagaggac acggcggtc 
agcaaactg ctgctgaac gtcgagtat cgcgtgcaa 
gggggccca tctgccgct tcgctccat actgataat 
actgcagaa ggggatatt atcaagaat ttgatcacg 
gaggggttg ctcctggtc gagaacaga aaggagaga 
cggcagaat aaattgctt ggcgcgtac aaggaggcc 
caggaagtg gccaagagg aaccattcg aacatttgg 
gagtatggt gacatcact gaagatgat gccaaggag 
tttggactg gggaaatga gggaccatt cattcagag 
cgacattaa cctacatta aattttaaa actagctgt 
gggttgcaa aacttctct agtagactt tccatttaa 
ctaggatag atgccaata taggatata tttttatcc 
agcaccatt attatacag atgttgatg tgtatgttt 
attttatat ggtttgtac aatatggaa attatgaat 
ggaatattg tggccttta cataatgtt ttacatttt 
tattaacac ttttatgtt taaattcgt tcacttgac 
gcattcaat tttactttt tatgtatat ttctggttg 
tgacaattt atttttaca cgatttcct gtatatttt 
ggctaatcg aacagctac ctttcgtat tatgtatat 
tttgtgccc tatgttaat cgtatctct tattcaaat 
ttatgttat ttataagtt atcaggtgt aatatcatt 
acttcaaat gatttgatt aataagtat aatccaact 
tgattcatt caagttttg agtgcatct ggtcatgac 
acacagact gggtataat tttatgtac gtactgtaa 
aacaatttt tacttcgat tgaaggcgt ggatgtaac 
taatgtagg gagtggctc tgtgtaaat taaataaaa 
gcattattg ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY