| General information | |||
| TC002936 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6040565..6046218 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002936 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00473 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (19 dpi)
remarks: later than schedule, hours 24
- >TC002936 RA
MTTQQNQPK RGIVKQILS GDSVIIRGP TGAPPPEKQ INFSGIVAP KLARRAGDQ SEPTKDEPW AWEAREFLR KKLIGEEVF FTSEKPPNA NREYGTVYL GKDFNSAEN ITESLVSEG LVTVRREGV RQSPEGARL AELEDAAKA AGKGKWGSS PPSEHVRDI KWSVENMRS FVDKLGYKP VKAIIEHVR DGSTVRAFL LPEFYHVTL MISGIRCPG FKLDANGKP DPSIKVPYA EEARYFVEI RLLQREVDI VLESVNNNN FVGTIIHPK GNIAEALLK EGFAHCVDW SIAFMKSGV EGLRAAEKK AKMARLRIW KDWQSNAPQ VTGKEKEFS ATVAEVING DALSVKLNN GQYKKIFLS SIRPPKEPG RVADEDGKT APRPKGFRP LYDIPWMFE AREYLRKKL IGKKVHVVI DYIQEARDG YPEKVCATV TVGGKNVAE ALVAKGLAS VVKYRPDDD QRSSKYDDL LAAESKAMK SGIGIHNKK DVPIHRVTE IDAARAKLE LSSFQRAQR IDAVVEFVA SGTRLRVFI PKSNSLCTF LLGGINCPR ASRQATNAQ PAVEGEPFG DEALQFTKE KCLQREVSI QVDTHDKAG NFIGWLWID NVNLSVALV KEGFASVHR TGEKSQYAA LLKEAEDSA KQHRLRIWK NYEEEKEEP HAEEEKPNV ERKVSYEEV VVTEVTPEG SFFVQTISE GPKAEALNA KLRQEFQAN PPLPGAYTP KRGDICAAK YTVDDEWYR VKVEKVQGG KASVHYIDY GNRETLPST RLASLPAAY AGEKPYATE YILPYVTLP KDEEYAAMA LKYLREDTA VSKLLLNVE YRVQGGPSA ASLHTDNTA EGDIIKNLI TEGLLLVEN RKERRQNKL LGAYKEAQE VAKRNHSNI WEYGDITED DAKEFGLGK
- >TC002936 RA
atgactacc caacaaaat cagccaaaa cgtggaatt gtgaaacaa atcctctct ggggattct gtaataatc cggggcccc accggagca cctcccccc gagaaacaa atcaatttt tcggggatc gtcgccccc aagttggcc cgcagagcc ggagaccag tccgaaccg acgaaagac gagccatgg gcttgggaa gctcgcgag ttcctccgc aaaaagctc atcggagag gaggttttc ttcacctca gagaagccc ccgaatgcc aatcgggag tacggcacg gtgtatttg ggcaaagac ttcaactcg gccgaaaac atcaccgag tcgttggtc tcggagggc cttgtgacg gtgcgtcgc gagggcgtc cggcagagc cctgagggg gcccgtctg gccgagctc gaggacgcc gccaaggcc gccggtaag ggtaaatgg ggcagcagc cccccttca gagcacgtc cgagacatc aaatggtcg gttgagaac atgcgctct ttcgtcgat aaattgggg tacaagccc gtgaaggcc atcattgaa cacgtccgc gatggctcc actgtccgt gcgttcctc ctccctgag ttctaccac gtcacgctt atgatttcg ggaattagg tgtcccggt tttaaactg gacgctaat ggtaagccc gaccccagc attaaggtt ccatatgct gaagaggct cgctatttc gttgaaatt cgtctcttg caaagagaa gttgatatt gttttggaa tcggtcaat aataataat tttgtgggt actattatc catccgaaa ggcaacatt gctgaagct ctgttgaaa gagggcttc gcccattgc gttgattgg tcgattgcg tttatgaag tcgggggtt gaaggtttg agagctgct gagaaaaaa gcgaaaatg gcccggctt cggatttgg aaagactgg caatcgaat gcaccgcag gttacgggc aaagaaaag gagttttcg gctacagtt gctgaagtt attaatgga gatgcactc tctgttaag ttgaacaat ggccaatat aagaagatc tttttgtcg agtataaga ccaccgaaa gagccaggc cgtgttgcc gatgaagac ggcaagact gccccgaga cctaaagga ttccgtcca ctgtacgat attccatgg atgtttgaa gcaagagag tacttacgc aagaaactc atcggtaag aaagtccac gtcgtcatt gactacatc caagaagcc agagacggt taccctgaa aaagtttgc gctactgtt acagttggt ggaaaaaat gttgccgaa gcacttgtt gcaaaaggt ctcgcgtcc gttgtgaaa taccgccct gatgacgat caacgtagc tccaaatac gatgatctt ttggctgcc gaaagcaaa gccatgaaa tcaggaatc gggattcat aacaagaag gacgttccg attcaccga gtgactgaa attgatgct gccagagcc aaactcgaa ttgtcttcc ttccaacgt gcacaaaga atcgacgcc gttgttgaa tttgtcgcc agtggaacc agattaaga gttttcata ccgaaatcg aacagttta tgtaccttt ttgttagga ggtataaat tgtccgcgc gcttcccgc caagcaacc aatgcccag cccgccgtc gaaggcgaa cctttcggt gatgaagca ttgcaattt acaaaggaa aagtgtctt cagagagaa gtttcgatt caagttgat actcacgac aaagccggc aatttcatt ggctggctc tggattgac aatgttaac ttatcggtc gctctcgtt aaagaaggt ttcgcttcc gtgcaccgc accggagaa aaatcacaa tatgctgcc ctcctcaaa gaagccgaa gacagtgcc aaacaacac cgtttacga atatggaag aactacgaa gaggagaaa gaggaacca cacgcagaa gaggagaaa cccaacgtt gagcgaaaa gtgagttac gaggaagtg gtggtgaca gaagtcacc cccgagggt tcgttcttc gtccaaacc atctccgag ggtcccaaa gccgaagcg ttgaacgcc aaacttcgc caagaattc caagccaat ccgcccctt cccggcgcc tacactcca aagcgtggc gacatctgt gctgccaaa tacaccgtt gacgacgag tggtacagg gttaaggtc gagaaggtg caagggggc aaggcctcc gttcactac atcgactac ggcaaccgc gaaacgctt ccaagcacc cgcctcgcc agtctaccc gccgcctac gcaggggag aagccctac gccaccgaa tacatcctg ccttacgtc accctgccc aaagacgag gaatacgct gcgatggcc ctcaagtat ctgcgagag gacacggcg gtcagcaaa ctgctgctg aacgtcgag tatcgcgtg caagggggc ccatctgcc gcttcgctc catactgat aatactgca gaaggggat attatcaag aatttgatc acggagggg ttgctcctg gtcgagaac agaaaggag agacggcag aataaattg cttggcgcg tacaaggag gcccaggaa gtggccaag aggaaccat tcgaacatt tgggagtat ggtgacatc actgaagat gatgccaag gagtttgga ctggggaaa tga
- >TC002936 RA
taccaaata aaatgataa atgggtggt acttacagt taggtttgc ggtcacgtt gagtcccaa attactgta caattttca agtggttcc aaagaaggg aatcaattt ttaacccac cccttgacg ctttcaccg agtcacgtg actgagtga cgttcatcc acctttcag tacaagttg catggcccg ttcacgatg actacccaa caaaatcag ccaaaacgt ggaattgtg aaacaaatc ctctctggg gattctgta ataatccgg ggccccacc ggagcacct ccccccgag aaacaaatc aatttttcg gggatcgtc gcccccaag ttggcccgc agagccgga gaccagtcc gaaccgacg aaagacgag ccatgggct tgggaagct cgcgagttc ctccgcaaa aagctcatc ggagaggag gttttcttc acctcagag aagcccccg aatgccaat cgggagtac ggcacggtg tatttgggc aaagacttc aactcggcc gaaaacatc accgagtcg ttggtctcg gagggcctt gtgacggtg cgtcgcgag ggcgtccgg cagagccct gagggggcc cgtctggcc gagctcgag gacgccgcc aaggccgcc ggtaagggt aaatggggc agcagcccc ccttcagag cacgtccga gacatcaaa tggtcggtt gagaacatg cgctctttc gtcgataaa ttggggtac aagcccgtg aaggccatc attgaacac gtccgcgat ggctccact gtccgtgcg ttcctcctc cctgagttc taccacgtc acgcttatg atttcggga attaggtgt cccggtttt aaactggac gctaatggt aagcccgac cccagcatt aaggttcca tatgctgaa gaggctcgc tatttcgtt gaaattcgt ctcttgcaa agagaagtt gatattgtt ttggaatcg gtcaataat aataatttt gtgggtact attatccat ccgaaaggc aacattgct gaagctctg ttgaaagag ggcttcgcc cattgcgtt gattggtcg attgcgttt atgaagtcg ggggttgaa ggtttgaga gctgctgag aaaaaagcg aaaatggcc cggcttcgg atttggaaa gactggcaa tcgaatgca ccgcaggtt acgggcaaa gaaaaggag ttttcggct acagttgct gaagttatt aatggagat gcactctct gttaagttg aacaatggc caatataag aagatcttt ttgtcgagt ataagacca ccgaaagag ccaggccgt gttgccgat gaagacggc aagactgcc ccgagacct aaaggattc cgtccactg tacgatatt ccatggatg tttgaagca agagagtac ttacgcaag aaactcatc ggtaagaaa gtccacgtc gtcattgac tacatccaa gaagccaga gacggttac cctgaaaaa gtttgcgct actgttaca gttggtgga aaaaatgtt gccgaagca cttgttgca aaaggtctc gcgtccgtt gtgaaatac cgccctgat gacgatcaa cgtagctcc aaatacgat gatcttttg gctgccgaa agcaaagcc atgaaatca ggaatcggg attcataac aagaaggac gttccgatt caccgagtg actgaaatt gatgctgcc agagccaaa ctcgaattg tcttccttc caacgtgca caaagaatc gacgccgtt gttgaattt gtcgccagt ggaaccaga ttaagagtt ttcataccg aaatcgaac agtttatgt acctttttg ttaggaggt ataaattgt ccgcgcgct tcccgccaa gcaaccaat gcccagccc gccgtcgaa ggcgaacct ttcggtgat gaagcattg caatttaca aaggaaaag tgtcttcag agagaagtt tcgattcaa gttgatact cacgacaaa gccggcaat ttcattggc tggctctgg attgacaat gttaactta tcggtcgct ctcgttaaa gaaggtttc gcttccgtg caccgcacc ggagaaaaa tcacaatat gctgccctc ctcaaagaa gccgaagac agtgccaaa caacaccgt ttacgaata tggaagaac tacgaagag gagaaagag gaaccacac gcagaagag gagaaaccc aacgttgag cgaaaagtg agttacgag gaagtggtg gtgacagaa gtcaccccc gagggttcg ttcttcgtc caaaccatc tccgagggt cccaaagcc gaagcgttg aacgccaaa cttcgccaa gaattccaa gccaatccg ccccttccc ggcgcctac actccaaag cgtggcgac atctgtgct gccaaatac accgttgac gacgagtgg tacagggtt aaggtcgag aaggtgcaa gggggcaag gcctccgtt cactacatc gactacggc aaccgcgaa acgcttcca agcacccgc ctcgccagt ctacccgcc gcctacgca ggggagaag ccctacgcc accgaatac atcctgcct tacgtcacc ctgcccaaa gacgaggaa tacgctgcg atggccctc aagtatctg cgagaggac acggcggtc agcaaactg ctgctgaac gtcgagtat cgcgtgcaa gggggccca tctgccgct tcgctccat actgataat actgcagaa ggggatatt atcaagaat ttgatcacg gaggggttg ctcctggtc gagaacaga aaggagaga cggcagaat aaattgctt ggcgcgtac aaggaggcc caggaagtg gccaagagg aaccattcg aacatttgg gagtatggt gacatcact gaagatgat gccaaggag tttggactg gggaaatga gggaccatt cattcagag cgacattaa cctacatta aattttaaa actagctgt gggttgcaa aacttctct agtagactt tccatttaa ctaggatag atgccaata taggatata tttttatcc agcaccatt attatacag atgttgatg tgtatgttt attttatat ggtttgtac aatatggaa attatgaat ggaatattg tggccttta cataatgtt ttacatttt tattaacac ttttatgtt taaattcgt tcacttgac gcattcaat tttactttt tatgtatat ttctggttg tgacaattt atttttaca cgatttcct gtatatttt ggctaatcg aacagctac ctttcgtat tatgtatat tttgtgccc tatgttaat cgtatctct tattcaaat ttatgttat ttataagtt atcaggtgt aatatcatt acttcaaat gatttgatt aataagtat aatccaact tgattcatt caagttttg agtgcatct ggtcatgac acacagact gggtataat tttatgtac gtactgtaa aacaatttt tacttcgat tgaaggcgt ggatgtaac taatgtagg gagtggctc tgtgtaaat taaataaaa gcattattg ttat
