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Gene information
General information
TC002924 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1638811..1646663
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002924
    iBeetle screen iB_00470
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002924 RA
      MKLLILDTP SDVSAWSAK YVAKRINEF NPGPDKHFV 
      LGLPTGGTP LGMYRKLID LYKKGEVSF RYVVTFNMD 
      EYVNLPRDH CQSYHFFMF DNFFKHIDI DPKNVHILD 
      GNAPDLMKE CKNYEKKIK EMGGVDLFI GGIGTDGHI 
      AFNEPGSSL VSRTRVKTL THDTLEANA RFFDNDISK 
      VPREALTVG VGTVMDARE VMILITGSH KAFALYKAI 
      EDGVNHMWT VSAFQLHPN TLMVCDEDA TMELRVKTV 
      KYFKGLNHH QLNETDSVA SIRS
    CDS sequence
    • >TC002924 RA
      atgaaactg ttaatactg gacactcca agcgacgtt 
      tcggcgtgg tctgccaaa tatgtcgct aaaagaatt 
      aacgaattt aacccaggt ccagacaag cactttgtc 
      cttggcctt ccgacaggt ggtacacct cttggtatg 
      taccggaag ctaatcgac ttgtacaaa aaaggagaa 
      gtgtccttt cgatatgtt gtaacgttc aatatggac 
      gaatatgtt aatctaccg cgggatcac tgccaaagt 
      taccacttc ttcatgttt gacaatttt ttcaaacat 
      atagacata gacccgaaa aatgtacat attttagac 
      ggaaatgca cccgatttg atgaaggaa tgcaaaaac 
      tatgagaag aaaattaaa gagatggga ggagtcgac 
      ctattcatc ggagggatt ggaacagat ggtcatatc 
      gccttcaat gagccggga tcatccttg gtttcccgt 
      actcgagtg aagactctc acacatgac actctagag 
      gccaatgct cggtttttt gataacgac atttccaaa 
      gtcccaaga gaagccctc actgttggc gtcggcacc 
      gtcatggac gccagagag gtgatgatt ttgataact 
      ggatcacat aaagcgttt gctttgtac aaggccatc 
      gaagatggg gtcaatcac atgtggact gtatccgca 
      tttcaactt catccaaac acgttaatg gtttgcgat 
      gaagatgcc acaatggaa ctcagagtg aagaccgtc 
      aaatatttt aaagggttg aaccatcat caacttaac 
      gaaaccgac agcgttgct agtatcagg agttga
    mRNA sequence
    • >TC002924 RA
      tgtgcttcc cgttggaga ttatttacg atatgatgc 
      tgtcaacgt ccgtttaaa aaaatgaaa ctgttaata 
      ctggacact ccaagcgac gtttcggcg tggtctgcc 
      aaatatgtc gctaaaaga attaacgaa tttaaccca 
      ggtccagac aagcacttt gtccttggc cttccgaca 
      ggtggtaca cctcttggt atgtaccgg aagctaatc 
      gacttgtac aaaaaagga gaagtgtcc tttcgatat 
      gttgtaacg ttcaatatg gacgaatat gttaatcta 
      ccgcgggat cactgccaa agttaccac ttcttcatg 
      tttgacaat tttttcaaa catatagac atagacccg 
      aaaaatgta catatttta gacggaaat gcacccgat 
      ttgatgaag gaatgcaaa aactatgag aagaaaatt 
      aaagagatg ggaggagtc gacctattc atcggaggg 
      attggaaca gatggtcat atcgccttc aatgagccg 
      ggatcatcc ttggtttcc cgtactcga gtgaagact 
      ctcacacat gacactcta gaggccaat gctcggttt 
      tttgataac gacatttcc aaagtccca agagaagcc 
      ctcactgtt ggcgtcggc accgtcatg gacgccaga 
      gaggtgatg attttgata actggatca cataaagcg 
      tttgctttg tacaaggcc atcgaagat ggggtcaat 
      cacatgtgg actgtatcc gcatttcaa cttcatcca 
      aacacgtta atggtttgc gatgaagat gccacaatg 
      gaactcaga gtgaagacc gtcaaatat tttaaaggg 
      ttgaaccat catcaactt aacgaaacc gacagcgtt 
      gctagtatc aggagttga aaacgaatg gtctatttt 
      attgttatc ctaactaaa taaaaactt taaagtgaa