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Gene information
General information
TC002920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1678599..1680753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002920
    iBeetle screen iB_00469
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva mostly everted (inside-out) -

    head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002920 RA
      MNPCKLKEP KVIDNALLE KCIKDQGPK GEAGRLAEI 
      EKIPLEDVT EIRLEFLNI LRIDHLWVL TSLTKLNLN 
      NNLLEKIEN LESLVNLTE LNLSFNKIA KIENLDELR 
      NLEKLSLYD NEITVLENM DTLTKLTVF SIGRNKIDD 
      LDNILYLRR FGNLKSLNL VGNPCAEDE DFRLFIAVF 
      LPQLVYYEY KLIYESERE LGHETFSAK LRALLEKEE 
      KEREITAIK EQELADAEL HASSFVEYL NTRHLFDSL 
      FANDTDGMN LMKIGEDAQ EFYDEYEEN FIELCKQIF 
      ENGQQQYIL RKTEQDQFL KCVEDANKD NQDESIKHM 
      EIFLEKKAI LFLKIKNVQ NQLNSNEIN YDEFIDKCD 
      VFVVEYDEM LHEVWKALM KLELELYEQ MEEVNQTFE 
      HNITELVNN FIESSQAFF TQIRELEVS YAENIGDLA 
      TKFQTNANL NEEIEVPAV LKPLMADKD LLHNALATS 
      HDMHMQIID AREDTLISN AKDWLNDLI DDLNTNEIK 
      RNRGKVLEI NHFLEIQRQ EFEDLNKEY APDLEDDVI 
      ALE
    CDS sequence
    • >TC002920 RA
      atgaatcct tgtaaattg aaagagcca aaagtaata 
      gacaatgcc cttctggag aaatgtatc aaagaccag 
      ggccccaag ggtgaagcg ggtcgcttg gcggagatc 
      gaaaaaatc ccccttgaa gacgtcacc gagattcgc 
      ttagaattt ctgaatatc ttaagaatt gaccacttg 
      tgggtgctg acatcttta acgaaatta aatctcaac 
      aataattta ctggaaaaa atcgaaaat ttggaaagt 
      ctggtgaac ttaactgaa ctaaattta tcattcaat 
      aaaatagct aaaatcgaa aatttagac gagcttcgc 
      aaccttgaa aagctgagt ctgtatgat aacgaaatc 
      acggttttg gagaacatg gacacttta acaaaatta 
      acagttttt agcattgga agaaacaaa attgacgac 
      ttggacaat attctgtat ttgcgacga ttcggcaat 
      ttaaaatcg ttaaatcta gtggggaat ccctgtgcc 
      gaggatgaa gattttcgt ttatttatt gcagttttt 
      ctgccacaa ctcgtttac tacgaatat aaattaata 
      tacgagtcg gaacgggaa cttggacac gaaactttc 
      agtgcaaaa ctgcgagca ttgttggag aaagaggag 
      aaggaacgg gaaatcact gcgataaag gaacaagag 
      ttggccgat gccgaactt cacgcctct agtttcgtg 
      gagtattta aacactcgc catttgttc gactcttta 
      tttgccaac gacaccgac ggcatgaac ttgatgaaa 
      atcggcgaa gacgcgcaa gaattctac gacgagtac 
      gaggagaac ttcatcgaa ctgtgtaaa caaatattc 
      gaaaatggg caacaacag tacatcctg cgcaaaacc 
      gaacaggac cagttctta aaatgcgtc gaagacgca 
      aataaggac aatcaagac gaaagcatt aaacacatg 
      gagatcttc ctggagaag aaggcaata ctgttcctc 
      aaaatcaaa aacgtgcaa aaccagctg aactcaaac 
      gaaataaat tacgacgag tttatcgac aaatgcgac 
      gtcttcgtg gtggagtac gacgagatg ttgcacgaa 
      gtttggaaa gcgctaatg aaactggaa ctggaactt 
      tacgaacaa atggaagaa gtcaaccaa actttcgaa 
      cacaacatc acagaactc gttaataat ttcattgaa 
      tcgtcgcag gctttcttt acgcagatc agagagctg 
      gaagtgagc tacgctgaa aatatcggc gatttggcc 
      acaaagttt caaacaaat gccaatttg aatgaggaa 
      atcgaagtt cccgcagtt cttaaaccg cttatggca 
      gataaggat cttctgcac aacgcgttg gcaacaagt 
      cacgatatg cacatgcag attatcgat gcgagagaa 
      gacactctc atttcgaac gccaaggat tggctaaat 
      gatttaatc gacgattta aacacgaat gagatcaag 
      cgaaacagg gggaaagtg ctggaaatt aaccacttt 
      cttgaaatc caaaggcag gagtttgag gatttgaat 
      aaggaatac gcgcctgat ctcgaggat gacgttata 
      gccttggaa taa
    mRNA sequence
    • >TC002920 RA
      agagttttt tgtaattga tataatttc acaatgaaa 
      aataataaa actcaagag cacaagctc ggcaaactc 
      aaagattca tgatttctc aagtccata gcaacaata 
      aggtactta aacaacagt cattgtgac atcaataat 
      ttcaagaat gcttgaaaa ggtatttgt atcgattta 
      gtgtgccta atgaaaatt tgtaaaaat gaatccttg 
      taaattgaa agagccaaa agtaataga caatgccct 
      tctggagaa atgtatcaa agaccaggg ccccaaggg 
      tgaagcggg tcgcttggc ggagatcga aaaaatccc 
      ccttgaaga cgtcaccga gattcgctt agaatttct 
      gaatatctt aagaattga ccacttgtg ggtgctgac 
      atctttaac gaaattaaa tctcaacaa taatttact 
      ggaaaaaat cgaaaattt ggaaagtct ggtgaactt 
      aactgaact aaatttatc attcaataa aatagctaa 
      aatcgaaaa tttagacga gcttcgcaa ccttgaaaa 
      gctgagtct gtatgataa cgaaatcac ggttttgga 
      gaacatgga cactttaac aaaattaac agtttttag 
      cattggaag aaacaaaat tgacgactt ggacaatat 
      tctgtattt gcgacgatt cggcaattt aaaatcgtt 
      aaatctagt ggggaatcc ctgtgccga ggatgaaga 
      ttttcgttt atttattgc agtttttct gccacaact 
      cgtttacta cgaatataa attaatata cgagtcgga 
      acgggaact tggacacga aactttcag tgcaaaact 
      gcgagcatt gttggagaa agaggagaa ggaacggga 
      aatcactgc gataaagga acaagagtt ggccgatgc 
      cgaacttca cgcctctag tttcgtgga gtatttaaa 
      cactcgcca tttgttcga ctctttatt tgccaacga 
      caccgacgg catgaactt gatgaaaat cggcgaaga 
      cgcgcaaga attctacga cgagtacga ggagaactt 
      catcgaact gtgtaaaca aatattcga aaatgggca 
      acaacagta catcctgcg caaaaccga acaggacca 
      gttcttaaa atgcgtcga agacgcaaa taaggacaa 
      tcaagacga aagcattaa acacatgga gatcttcct 
      ggagaagaa ggcaatact gttcctcaa aatcaaaaa 
      cgtgcaaaa ccagctgaa ctcaaacga aataaatta 
      cgacgagtt tatcgacaa atgcgacgt cttcgtggt 
      ggagtacga cgagatgtt gcacgaagt ttggaaagc 
      gctaatgaa actggaact ggaacttta cgaacaaat 
      ggaagaagt caaccaaac tttcgaaca caacatcac 
      agaactcgt taataattt cattgaatc gtcgcaggc 
      tttctttac gcagatcag agagctgga agtgagcta 
      cgctgaaaa tatcggcga tttggccac aaagtttca 
      aacaaatgc caatttgaa tgaggaaat cgaagttcc 
      cgcagttct taaaccgct tatggcaga taaggatct 
      tctgcacaa cgcgttggc aacaagtca cgatatgca 
      catgcagat tatcgatgc gagagaaga cactctcat 
      ttcgaacgc caaggattg gctaaatga tttaatcga 
      cgatttaaa cacgaatga gatcaagcg aaacagggg 
      gaaagtgct ggaaattaa ccactttct tgaaatcca 
      aaggcagga gtttgagga tttgaataa ggaatacgc 
      gcctgatct cgaggatga cgttatagc cttggaata 
      agtaagata gtaaaatat acttttatt tttaca