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Gene information
General information
TC002911 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2037985..2045476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002911
    iBeetle screen iB_00466
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition 3 number 2nd lateral oviduct: 4 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: animals lost, remarks: animals lost, adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost, remarks: animals lost,
    Adult morphology
    remarks: animals lost, remarks: animals lost,
    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002911 RA
      MFIKLSVII SFLHLTIAQ TQNHDWELA VVTSDKIEL 
      LLSNGSMIG SAFEQFNKL KALTFDNIR HQFVVSDMD 
      QQNDTIFSV QLTKETDVT SIIEDLPDD VQGLAIDPI 
      SDILYWTDS INKTINYVR LNEPAYKSN ILFAFTDES 
      PQDITIDVC RRYIYWTNS DINKPTIER ARLDGSQHE 
      VLIDTGLHR PAGISIDYK TQRLFWADV KEGIYYSIE 
      STNLEGKER EVVYEGTHS KPFGVAVDS DAVYWTDIN 
      NNVLWKINK HDKDSVPER LRHFKEKPM GIIAKNVQV 
      SSFPDCEEL SKAISSYNK SVEEVFKHG SQESDEEPE 
      EAVCLNGGF LVDGKFCHC PRGYAGKSC EISLCYNYC 
      VHGECYASS LGYPQCHCL KGFGGKRCS KDMCDGFCL 
      NGGVCKRGG ELPVCECQE GFTGRRCES TLDICEAFC 
      QDRQNDVLI TQKEVVCRC VDGYPDVVK SNFSSLPLL 
      SSQDEHPAF FDHFQDTAF LVLCCLVFI LLVVSVVLA 
      AFACSLRRR RPRIKKRII VNKNFTQLT NRPRASEQC 
      EITIENCCN MNICETPCF EPPQLPCLQ NKNDDKKKL 
      LSNMENGEE LC
    CDS sequence
    • >TC002911 RA
      atgtttatc aaattaagt gttatcatt tctttcctt 
      catcttacc atcgcccag acgcaaaat cacgactgg 
      gagttggct gtagtgacg agcgataaa atcgaattg 
      ttgttatca aatgggtcg atgattggg tcagcgttt 
      gaacaattc aacaaactg aaagcacta acgtttgat 
      aacatacgg catcagttc gttgtcagt gacatggac 
      caacaaaac gacacgatc ttttctgtt caacttacc 
      aaagagact gatgtgaca tccatcatc gaagatctt 
      ccagacgat gtccaggga ttagcgata gatcccatt 
      tctgatatt ttatactgg accgattca attaataag 
      accattaat tatgtacgt ttaaacgaa cctgcttac 
      aaatcaaac attcttttt gcgtttaca gatgagagt 
      ccgcaggac attaccatc gatgtgtgt cgccggtac 
      atttactgg acgaactca gatatcaac aagccaacg 
      attgaaaga gccagactg gacggttcc cagcatgag 
      gtcttgata gacacaggt ctgcacaga cctgcgggc 
      atctcaatc gactacaaa acgcaaaga ctcttctgg 
      gccgatgtc aaggaaggc atttattac agcatcgaa 
      agtacaaat ttggagggc aaagagcgc gaagttgtg 
      tatgaaggg acgcactcg aagcccttc ggcgtggct 
      gttgactca gacgccgtc tactggacg gacatcaac 
      aacaacgtc ttgtggaag ataaacaag cacgacaaa 
      gactcagtt cctgaacgc cttcgtcac ttcaaggag 
      aaacccatg ggaatcatt gccaaaaac gtgcaagtc 
      tctagtttc cccgactgt gaggagctg tcaaaagcc 
      ataagtagt tacaacaag tcggtggag gaggtgttc 
      aagcacggg tcgcaagag agtgacgaa gagccagaa 
      gaggcggtt tgcctcaat ggtgggttt ctagtcgac 
      ggtaaattc tgccattgt cctcgcggt tacgctggg 
      aaatcctgc gaaatatct ttatgctac aattattgt 
      gttcatggc gagtgctat gcctcgtct ctgggatat 
      cctcaatgt cactgcctc aagggtttt ggggggaag 
      aggtgcagc aaggatatg tgtgacggg ttttgctta 
      aatggagga gtgtgcaaa cgaggagga gaattgcct 
      gtgtgcgag tgccaggag ggattcact ggccgcaga 
      tgtgaaagt acacttgac atctgcgag gcgttttgt 
      caagaccgg caaaatgat gttttgata acacagaaa 
      gaagttgtt tgtagatgt gtggatgga tatcctgac 
      gttgttaag tcaaatttt tcctcactt ccactgtta 
      tcgtctcaa gacgagcat ccagccttt ttcgaccac 
      ttccaagac acggcattt ttagtcctg tgttgtctg 
      gtgttcata ttgttggtt gtgtcggtc gtgttggca 
      gcttttgct tgctccttg cgtagaagg agaccgagg 
      attaagaag cgaattata gtgaataaa aatttcaca 
      caactgact aacaggcca cgagcgtca gaacagtgt 
      gaaataact atagaaaat tgctgcaat atgaatatt 
      tgtgaaacg ccttgtttt gagccgcct caactgcca 
      tgtctgcaa aacaagaac gacgacaag aagaaattg 
      ctatcgaat atggaaaac ggtgaagaa ctgtgctga
    mRNA sequence
    • >TC002911 RA
      ctcgggcgc agtgtgagt tcacgtgct taaatgata 
      tcgtaaaga cctctttta tttttagta cttaaatta 
      ttttttaaa aaaatgttt atcaaatta agtgttatc 
      atttctttc cttcatctt accatcgcc cagacgcaa 
      aatcacgac tgggagttg gctgtagtg acgagcgat 
      aaaatcgaa ttgttgtta tcaaatggg tcgatgatt 
      gggtcagcg tttgaacaa ttcaacaaa ctgaaagca 
      ctaacgttt gataacata cggcatcag ttcgttgtc 
      agtgacatg gaccaacaa aacgacacg atcttttct 
      gttcaactt accaaagag actgatgtg acatccatc 
      atcgaagat cttccagac gatgtccag ggattagcg 
      atagatccc atttctgat attttatac tggaccgat 
      tcaattaat aagaccatt aattatgta cgtttaaac 
      gaacctgct tacaaatca aacattctt tttgcgttt 
      acagatgag agtccgcag gacattacc atcgatgtg 
      tgtcgccgg tacatttac tggacgaac tcagatatc 
      aacaagcca acgattgaa agagccaga ctggacggt 
      tcccagcat gaggtcttg atagacaca ggtctgcac 
      agacctgcg ggcatctca atcgactac aaaacgcaa 
      agactcttc tgggccgat gtcaaggaa ggcatttat 
      tacagcatc gaaagtaca aatttggag ggcaaagag 
      cgcgaagtt gtgtatgaa gggacgcac tcgaagccc 
      ttcggcgtg gctgttgac tcagacgcc gtctactgg 
      acggacatc aacaacaac gtcttgtgg aagataaac 
      aagcacgac aaagactca gttcctgaa cgccttcgt 
      cacttcaag gagaaaccc atgggaatc attgccaaa 
      aacgtgcaa gtctctagt ttccccgac tgtgaggag 
      ctgtcaaaa gccataagt agttacaac aagtcggtg 
      gaggaggtg ttcaagcac gggtcgcaa gagagtgac 
      gaagagcca gaagaggcg gtttgcctc aatggtggg 
      tttctagtc gacggtaaa ttctgccat tgtcctcgc 
      ggttacgct gggaaatcc tgcgaaata tctttatgc 
      tacaattat tgtgttcat ggcgagtgc tatgcctcg 
      tctctggga tatcctcaa tgtcactgc ctcaagggt 
      tttgggggg aagaggtgc agcaaggat atgtgtgac 
      gggttttgc ttaaatgga ggagtgtgc aaacgagga 
      ggagaattg cctgtgtgc gagtgccag gagggattc 
      actggccgc agatgtgaa agtacactt gacatctgc 
      gaggcgttt tgtcaagac cggcaaaat gatgttttg 
      ataacacag aaagaagtt gtttgtaga tgtgtggat 
      ggatatcct gacgttgtt aagtcaaat ttttcctca 
      cttccactg ttatcgtct caagacgag catccagcc 
      tttttcgac cacttccaa gacacggca tttttagtc 
      ctgtgttgt ctggtgttc atattgttg gttgtgtcg 
      gtcgtgttg gcagctttt gcttgctcc ttgcgtaga 
      aggagaccg aggattaag aagcgaatt atagtgaat 
      aaaaatttc acacaactg actaacagg ccacgagcg 
      tcagaacag tgtgaaata actatagaa aattgctgc 
      aatatgaat atttgtgaa acgccttgt tttgagccg 
      cctcaactg ccatgtctg caaaacaag aacgacgac 
      aagaagaaa ttgctatcg aatatggaa aacggtgaa 
      gaactgtgc tgaaactat ctatattag ataggctgt 
      gacttccaa aaagtatta agttgttaa gtttgctat 
      ttatattag aaacgaaga ctaagtaaa tatctgacg 
      taaaaccga gcactgcac acaaaaccg cgttatata 
      atatttata taattcatt tacgggcaa ctttgcgtt 
      ttgtgtgtt gttcctctt ataattttg acttgaaac 
      acattcctg gaatcctgt gtgactcga aaatgtttc 
      catgacaag acaaatatt catacgtaa gaaccttgg 
      tttattcga ggtcgtgga caccgacca ttctgcgtt 
      tcacaaatc tttagcaat caaactgca gcaagagtt 
      gtggtactt aagatacaa ccaaaaaat tgttaactg 
      acaccaaaa attgggact cgttttttg tatttggtg 
      caataggtt tacaaacta attgggtca gtttatttg 
      tgatagtga aaattttgg cgtagctga acgaatttt 
      tattttagt tgttttatt tgctgaatc ttaaatttt 
      tcttagttt caaggttgt gagtaggtt agttaaatt 
      ggccaaata gcaactgat aaaaggtgc ctgtaaggt 
      ttgaagtag caagaaatg ataattgtg aataattgt 
      ttgcagaca taatcatca tttcgggcc tagaatcat 
      gtaatgagt ctaattgga tgtagcaat tttttattg 
      attcattta aaataaatc aagtcttaa tggtgcatc 
      catttaaat tacttaagt gggtgtggt ttgtaatat 
      ccacatcag aaatgtgat attataact atgtagctc 
      tatttttca aatgtatat aaatttttt aataaacaa 
      aaaatgttg aa