General information | |||
TC002911 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6599272..6606763 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC002911 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00466 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
remarks: animals lost, remarks: animals lost, adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 4
remarks: animals lost,
- >TC002911 RA
MFIKLSVII SFLHLTIAQ TQNHDWELA VVTSDKIEL LLSNGSMIG SAFEQFNKL KALTFDNIR HQFVVSDMD QQNDTIFSV QLTKETDVT SIIEDLPDD VQGLAIDPI SDILYWTDS INKTINYVR LNEPAYKSN ILFAFTDES PQDITIDVC RRYIYWTNS DINKPTIER ARLDGSQHE VLIDTGLHR PAGISIDYK TQRLFWADV KEGIYYSIE STNLEGKER EVVYEGTHS KPFGVAVDS DAVYWTDIN NNVLWKINK HDKDSVPER LRHFKEKPM GIIAKNVQV SSFPDCEEL SKAISSYNK SVEEVFKHG SQESDEEPE EAVCLNGGF LVDGKFCHC PRGYAGKSC EISLCYNYC VHGECYASS LGYPQCHCL KGFGGKRCS KDMCDGFCL NGGVCKRGG ELPVCECQE GFTGRRCES TLDICEAFC QDRQNDVLI TQKEVVCRC VDGYPDVVK SNFSSLPLL SSQDEHPAF FDHFQDTAF LVLCCLVFI LLVVSVVLA AFACSLRRR RPRIKKRII VNKNFTQLT NRPRASEQC EITIENCCN MNICETPCF EPPQLPCLQ NKNDDKKKL LSNMENGEE LC
- >TC002911 RA
atgtttatc aaattaagt gttatcatt tctttcctt catcttacc atcgcccag acgcaaaat cacgactgg gagttggct gtagtgacg agcgataaa atcgaattg ttgttatca aatgggtcg atgattggg tcagcgttt gaacaattc aacaaactg aaagcacta acgtttgat aacatacgg catcagttc gttgtcagt gacatggac caacaaaac gacacgatc ttttctgtt caacttacc aaagagact gatgtgaca tccatcatc gaagatctt ccagacgat gtccaggga ttagcgata gatcccatt tctgatatt ttatactgg accgattca attaataag accattaat tatgtacgt ttaaacgaa cctgcttac aaatcaaac attcttttt gcgtttaca gatgagagt ccgcaggac attaccatc gatgtgtgt cgccggtac atttactgg acgaactca gatatcaac aagccaacg attgaaaga gccagactg gacggttcc cagcatgag gtcttgata gacacaggt ctgcacaga cctgcgggc atctcaatc gactacaaa acgcaaaga ctcttctgg gccgatgtc aaggaaggc atttattac agcatcgaa agtacaaat ttggagggc aaagagcgc gaagttgtg tatgaaggg acgcactcg aagcccttc ggcgtggct gttgactca gacgccgtc tactggacg gacatcaac aacaacgtc ttgtggaag ataaacaag cacgacaaa gactcagtt cctgaacgc cttcgtcac ttcaaggag aaacccatg ggaatcatt gccaaaaac gtgcaagtc tctagtttc cccgactgt gaggagctg tcaaaagcc ataagtagt tacaacaag tcggtggag gaggtgttc aagcacggg tcgcaagag agtgacgaa gagccagaa gaggcggtt tgcctcaat ggtgggttt ctagtcgac ggtaaattc tgccattgt cctcgcggt tacgctggg aaatcctgc gaaatatct ttatgctac aattattgt gttcatggc gagtgctat gcctcgtct ctgggatat cctcaatgt cactgcctc aagggtttt ggggggaag aggtgcagc aaggatatg tgtgacggg ttttgctta aatggagga gtgtgcaaa cgaggagga gaattgcct gtgtgcgag tgccaggag ggattcact ggccgcaga tgtgaaagt acacttgac atctgcgag gcgttttgt caagaccgg caaaatgat gttttgata acacagaaa gaagttgtt tgtagatgt gtggatgga tatcctgac gttgttaag tcaaatttt tcctcactt ccactgtta tcgtctcaa gacgagcat ccagccttt ttcgaccac ttccaagac acggcattt ttagtcctg tgttgtctg gtgttcata ttgttggtt gtgtcggtc gtgttggca gcttttgct tgctccttg cgtagaagg agaccgagg attaagaag cgaattata gtgaataaa aatttcaca caactgact aacaggcca cgagcgtca gaacagtgt gaaataact atagaaaat tgctgcaat atgaatatt tgtgaaacg ccttgtttt gagccgcct caactgcca tgtctgcaa aacaagaac gacgacaag aagaaattg ctatcgaat atggaaaac ggtgaagaa ctgtgctga
- >TC002911 RA
ctcgggcgc agtgtgagt tcacgtgct taaatgata tcgtaaaga cctctttta tttttagta cttaaatta ttttttaaa aaaatgttt atcaaatta agtgttatc atttctttc cttcatctt accatcgcc cagacgcaa aatcacgac tgggagttg gctgtagtg acgagcgat aaaatcgaa ttgttgtta tcaaatggg tcgatgatt gggtcagcg tttgaacaa ttcaacaaa ctgaaagca ctaacgttt gataacata cggcatcag ttcgttgtc agtgacatg gaccaacaa aacgacacg atcttttct gttcaactt accaaagag actgatgtg acatccatc atcgaagat cttccagac gatgtccag ggattagcg atagatccc atttctgat attttatac tggaccgat tcaattaat aagaccatt aattatgta cgtttaaac gaacctgct tacaaatca aacattctt tttgcgttt acagatgag agtccgcag gacattacc atcgatgtg tgtcgccgg tacatttac tggacgaac tcagatatc aacaagcca acgattgaa agagccaga ctggacggt tcccagcat gaggtcttg atagacaca ggtctgcac agacctgcg ggcatctca atcgactac aaaacgcaa agactcttc tgggccgat gtcaaggaa ggcatttat tacagcatc gaaagtaca aatttggag ggcaaagag cgcgaagtt gtgtatgaa gggacgcac tcgaagccc ttcggcgtg gctgttgac tcagacgcc gtctactgg acggacatc aacaacaac gtcttgtgg aagataaac aagcacgac aaagactca gttcctgaa cgccttcgt cacttcaag gagaaaccc atgggaatc attgccaaa aacgtgcaa gtctctagt ttccccgac tgtgaggag ctgtcaaaa gccataagt agttacaac aagtcggtg gaggaggtg ttcaagcac gggtcgcaa gagagtgac gaagagcca gaagaggcg gtttgcctc aatggtggg tttctagtc gacggtaaa ttctgccat tgtcctcgc ggttacgct gggaaatcc tgcgaaata tctttatgc tacaattat tgtgttcat ggcgagtgc tatgcctcg tctctggga tatcctcaa tgtcactgc ctcaagggt tttgggggg aagaggtgc agcaaggat atgtgtgac gggttttgc ttaaatgga ggagtgtgc aaacgagga ggagaattg cctgtgtgc gagtgccag gagggattc actggccgc agatgtgaa agtacactt gacatctgc gaggcgttt tgtcaagac cggcaaaat gatgttttg ataacacag aaagaagtt gtttgtaga tgtgtggat ggatatcct gacgttgtt aagtcaaat ttttcctca cttccactg ttatcgtct caagacgag catccagcc tttttcgac cacttccaa gacacggca tttttagtc ctgtgttgt ctggtgttc atattgttg gttgtgtcg gtcgtgttg gcagctttt gcttgctcc ttgcgtaga aggagaccg aggattaag aagcgaatt atagtgaat aaaaatttc acacaactg actaacagg ccacgagcg tcagaacag tgtgaaata actatagaa aattgctgc aatatgaat atttgtgaa acgccttgt tttgagccg cctcaactg ccatgtctg caaaacaag aacgacgac aagaagaaa ttgctatcg aatatggaa aacggtgaa gaactgtgc tgaaactat ctatattag ataggctgt gacttccaa aaagtatta agttgttaa gtttgctat ttatattag aaacgaaga ctaagtaaa tatctgacg taaaaccga gcactgcac acaaaaccg cgttatata atatttata taattcatt tacgggcaa ctttgcgtt ttgtgtgtt gttcctctt ataattttg acttgaaac acattcctg gaatcctgt gtgactcga aaatgtttc catgacaag acaaatatt catacgtaa gaaccttgg tttattcga ggtcgtgga caccgacca ttctgcgtt tcacaaatc tttagcaat caaactgca gcaagagtt gtggtactt aagatacaa ccaaaaaat tgttaactg acaccaaaa attgggact cgttttttg tatttggtg caataggtt tacaaacta attgggtca gtttatttg tgatagtga aaattttgg cgtagctga acgaatttt tattttagt tgttttatt tgctgaatc ttaaatttt tcttagttt caaggttgt gagtaggtt agttaaatt ggccaaata gcaactgat aaaaggtgc ctgtaaggt ttgaagtag caagaaatg ataattgtg aataattgt ttgcagaca taatcatca tttcgggcc tagaatcat gtaatgagt ctaattgga tgtagcaat tttttattg attcattta aaataaatc aagtcttaa tggtgcatc catttaaat tacttaagt gggtgtggt ttgtaatat ccacatcag aaatgtgat attataact atgtagctc tatttttca aatgtatat aaatttttt aataaacaa aaaatgttg aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
