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Gene information
General information
TC002911 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6599272..6606763
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002911
    iBeetle screen iB_00466

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition 3 number 2nd lateral oviduct: 4 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    remarks: animals lost, remarks: animals lost, adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Pupal morphology
    remarks: animals lost, remarks: animals lost,
    Adult morphology
    remarks: animals lost, remarks: animals lost,
    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002911 RA
      MFIKLSVII SFLHLTIAQ TQNHDWELA VVTSDKIEL 
LLSNGSMIG SAFEQFNKL KALTFDNIR HQFVVSDMD 
QQNDTIFSV QLTKETDVT SIIEDLPDD VQGLAIDPI 
SDILYWTDS INKTINYVR LNEPAYKSN ILFAFTDES 
PQDITIDVC RRYIYWTNS DINKPTIER ARLDGSQHE 
VLIDTGLHR PAGISIDYK TQRLFWADV KEGIYYSIE 
STNLEGKER EVVYEGTHS KPFGVAVDS DAVYWTDIN 
NNVLWKINK HDKDSVPER LRHFKEKPM GIIAKNVQV 
SSFPDCEEL SKAISSYNK SVEEVFKHG SQESDEEPE 
EAVCLNGGF LVDGKFCHC PRGYAGKSC EISLCYNYC 
VHGECYASS LGYPQCHCL KGFGGKRCS KDMCDGFCL 
NGGVCKRGG ELPVCECQE GFTGRRCES TLDICEAFC 
QDRQNDVLI TQKEVVCRC VDGYPDVVK SNFSSLPLL 
SSQDEHPAF FDHFQDTAF LVLCCLVFI LLVVSVVLA 
AFACSLRRR RPRIKKRII VNKNFTQLT NRPRASEQC 
EITIENCCN MNICETPCF EPPQLPCLQ NKNDDKKKL 
LSNMENGEE LC
    CDS sequence
    • >TC002911 RA
      atgtttatc aaattaagt gttatcatt tctttcctt 
catcttacc atcgcccag acgcaaaat cacgactgg 
gagttggct gtagtgacg agcgataaa atcgaattg 
ttgttatca aatgggtcg atgattggg tcagcgttt 
gaacaattc aacaaactg aaagcacta acgtttgat 
aacatacgg catcagttc gttgtcagt gacatggac 
caacaaaac gacacgatc ttttctgtt caacttacc 
aaagagact gatgtgaca tccatcatc gaagatctt 
ccagacgat gtccaggga ttagcgata gatcccatt 
tctgatatt ttatactgg accgattca attaataag 
accattaat tatgtacgt ttaaacgaa cctgcttac 
aaatcaaac attcttttt gcgtttaca gatgagagt 
ccgcaggac attaccatc gatgtgtgt cgccggtac 
atttactgg acgaactca gatatcaac aagccaacg 
attgaaaga gccagactg gacggttcc cagcatgag 
gtcttgata gacacaggt ctgcacaga cctgcgggc 
atctcaatc gactacaaa acgcaaaga ctcttctgg 
gccgatgtc aaggaaggc atttattac agcatcgaa 
agtacaaat ttggagggc aaagagcgc gaagttgtg 
tatgaaggg acgcactcg aagcccttc ggcgtggct 
gttgactca gacgccgtc tactggacg gacatcaac 
aacaacgtc ttgtggaag ataaacaag cacgacaaa 
gactcagtt cctgaacgc cttcgtcac ttcaaggag 
aaacccatg ggaatcatt gccaaaaac gtgcaagtc 
tctagtttc cccgactgt gaggagctg tcaaaagcc 
ataagtagt tacaacaag tcggtggag gaggtgttc 
aagcacggg tcgcaagag agtgacgaa gagccagaa 
gaggcggtt tgcctcaat ggtgggttt ctagtcgac 
ggtaaattc tgccattgt cctcgcggt tacgctggg 
aaatcctgc gaaatatct ttatgctac aattattgt 
gttcatggc gagtgctat gcctcgtct ctgggatat 
cctcaatgt cactgcctc aagggtttt ggggggaag 
aggtgcagc aaggatatg tgtgacggg ttttgctta 
aatggagga gtgtgcaaa cgaggagga gaattgcct 
gtgtgcgag tgccaggag ggattcact ggccgcaga 
tgtgaaagt acacttgac atctgcgag gcgttttgt 
caagaccgg caaaatgat gttttgata acacagaaa 
gaagttgtt tgtagatgt gtggatgga tatcctgac 
gttgttaag tcaaatttt tcctcactt ccactgtta 
tcgtctcaa gacgagcat ccagccttt ttcgaccac 
ttccaagac acggcattt ttagtcctg tgttgtctg 
gtgttcata ttgttggtt gtgtcggtc gtgttggca 
gcttttgct tgctccttg cgtagaagg agaccgagg 
attaagaag cgaattata gtgaataaa aatttcaca 
caactgact aacaggcca cgagcgtca gaacagtgt 
gaaataact atagaaaat tgctgcaat atgaatatt 
tgtgaaacg ccttgtttt gagccgcct caactgcca 
tgtctgcaa aacaagaac gacgacaag aagaaattg 
ctatcgaat atggaaaac ggtgaagaa ctgtgctga
    mRNA sequence
    • >TC002911 RA
      ctcgggcgc agtgtgagt tcacgtgct taaatgata 
tcgtaaaga cctctttta tttttagta cttaaatta 
ttttttaaa aaaatgttt atcaaatta agtgttatc 
atttctttc cttcatctt accatcgcc cagacgcaa 
aatcacgac tgggagttg gctgtagtg acgagcgat 
aaaatcgaa ttgttgtta tcaaatggg tcgatgatt 
gggtcagcg tttgaacaa ttcaacaaa ctgaaagca 
ctaacgttt gataacata cggcatcag ttcgttgtc 
agtgacatg gaccaacaa aacgacacg atcttttct 
gttcaactt accaaagag actgatgtg acatccatc 
atcgaagat cttccagac gatgtccag ggattagcg 
atagatccc atttctgat attttatac tggaccgat 
tcaattaat aagaccatt aattatgta cgtttaaac 
gaacctgct tacaaatca aacattctt tttgcgttt 
acagatgag agtccgcag gacattacc atcgatgtg 
tgtcgccgg tacatttac tggacgaac tcagatatc 
aacaagcca acgattgaa agagccaga ctggacggt 
tcccagcat gaggtcttg atagacaca ggtctgcac 
agacctgcg ggcatctca atcgactac aaaacgcaa 
agactcttc tgggccgat gtcaaggaa ggcatttat 
tacagcatc gaaagtaca aatttggag ggcaaagag 
cgcgaagtt gtgtatgaa gggacgcac tcgaagccc 
ttcggcgtg gctgttgac tcagacgcc gtctactgg 
acggacatc aacaacaac gtcttgtgg aagataaac 
aagcacgac aaagactca gttcctgaa cgccttcgt 
cacttcaag gagaaaccc atgggaatc attgccaaa 
aacgtgcaa gtctctagt ttccccgac tgtgaggag 
ctgtcaaaa gccataagt agttacaac aagtcggtg 
gaggaggtg ttcaagcac gggtcgcaa gagagtgac 
gaagagcca gaagaggcg gtttgcctc aatggtggg 
tttctagtc gacggtaaa ttctgccat tgtcctcgc 
ggttacgct gggaaatcc tgcgaaata tctttatgc 
tacaattat tgtgttcat ggcgagtgc tatgcctcg 
tctctggga tatcctcaa tgtcactgc ctcaagggt 
tttgggggg aagaggtgc agcaaggat atgtgtgac 
gggttttgc ttaaatgga ggagtgtgc aaacgagga 
ggagaattg cctgtgtgc gagtgccag gagggattc 
actggccgc agatgtgaa agtacactt gacatctgc 
gaggcgttt tgtcaagac cggcaaaat gatgttttg 
ataacacag aaagaagtt gtttgtaga tgtgtggat 
ggatatcct gacgttgtt aagtcaaat ttttcctca 
cttccactg ttatcgtct caagacgag catccagcc 
tttttcgac cacttccaa gacacggca tttttagtc 
ctgtgttgt ctggtgttc atattgttg gttgtgtcg 
gtcgtgttg gcagctttt gcttgctcc ttgcgtaga 
aggagaccg aggattaag aagcgaatt atagtgaat 
aaaaatttc acacaactg actaacagg ccacgagcg 
tcagaacag tgtgaaata actatagaa aattgctgc 
aatatgaat atttgtgaa acgccttgt tttgagccg 
cctcaactg ccatgtctg caaaacaag aacgacgac 
aagaagaaa ttgctatcg aatatggaa aacggtgaa 
gaactgtgc tgaaactat ctatattag ataggctgt 
gacttccaa aaagtatta agttgttaa gtttgctat 
ttatattag aaacgaaga ctaagtaaa tatctgacg 
taaaaccga gcactgcac acaaaaccg cgttatata 
atatttata taattcatt tacgggcaa ctttgcgtt 
ttgtgtgtt gttcctctt ataattttg acttgaaac 
acattcctg gaatcctgt gtgactcga aaatgtttc 
catgacaag acaaatatt catacgtaa gaaccttgg 
tttattcga ggtcgtgga caccgacca ttctgcgtt 
tcacaaatc tttagcaat caaactgca gcaagagtt 
gtggtactt aagatacaa ccaaaaaat tgttaactg 
acaccaaaa attgggact cgttttttg tatttggtg 
caataggtt tacaaacta attgggtca gtttatttg 
tgatagtga aaattttgg cgtagctga acgaatttt 
tattttagt tgttttatt tgctgaatc ttaaatttt 
tcttagttt caaggttgt gagtaggtt agttaaatt 
ggccaaata gcaactgat aaaaggtgc ctgtaaggt 
ttgaagtag caagaaatg ataattgtg aataattgt 
ttgcagaca taatcatca tttcgggcc tagaatcat 
gtaatgagt ctaattgga tgtagcaat tttttattg 
attcattta aaataaatc aagtcttaa tggtgcatc 
catttaaat tacttaagt gggtgtggt ttgtaatat 
ccacatcag aaatgtgat attataact atgtagctc 
tatttttca aatgtatat aaatttttt aataaacaa 
aaaatgttg aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY