Status
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Gene information
General information
TC002887 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7246024..7249441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002887
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00463

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber shape rounded - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    remarks: later than schedule, hours 24
    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 7

    wings not closed - Number of animals showing the phenotype: 7
    oblique dorsal thoracic muscles number increased - Number of animals showing the phenotype: 5

    longitudinal dorsal thoracic muscles number increased - Number of animals showing the phenotype: 5
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 5
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002887 RA
      MNNQNLDLK SAWITIKEA FPILESPSK IPDNDFDSA 
PFEAISIIK RLNLTQQIQ DLVIIKIEE RLRKEIVPD 
FWSHFKKTE NEAAVFHEF YSVVKFLFH NYVQLDLII 
NKLILLREA SNFTKPVYN QTCLHEALK LIVRAVLLS 
QLPIDHEWM IVNFYETAL KMQDPEEEN STSGECVVC 
MQEEFSCSC REMFEETNK KLGKMTLLE PLVGQALTD 
LIYSYIQTH IQKICKDNF DNSHIESLE KWLQTVVIS 
WLKKIYFHN SAVKAMDEF DKKLINYLY NTYTKIRID 
QLFNIIIEY PDSLPALDD LRLCLPKTD LKPHLTRSL 
QKAIETRLL HPGVSTFDV LTAYVATIR SLRILDPCG 
LLLETITQP VHQYLRSRE DTIRCVVGS LTEEGPNDL 
AEELVRGEA VQVDENISV DEGVEDWET WTPDPVDVV 
PSKSSGLPR TSDIISMLV NVYGSKELF VNEYRTLLA 
DRLLSQYSC DTEKEIRYL ELLKLRFGD SQLHFCEVM 
LKDIADSKR INQHIQQDL SYSDDDIPM SAMILSAQF 
WPAFKDEKL ELHEKLVKQ MESYTGAFE TLKGSRTLC 
WKNHLGIVD LEIELADRT LNLSVSPVH ATILMHFQD 
QNTWSLEAL SKVMHVPST ILRRKLSFW QSHGIITEP 
EPDVFYLQE EQENNENNI QDDIVVEDF ESESAMASA 
QDQREEELQ TFWSYILGM LMNLDTLPL ERIHQMLKM 
FAFQGPTIE CNLQELKVF LDRKVREHQ LIYSNGLYR 
LPK
    CDS sequence
    • >TC002887 RA
      atgaataat caaaacctg gacttaaaa agtgcgtgg 
attacgatt aaagaagct tttccaatc ttagaaagt 
ccttcaaag atccctgat aatgatttc gactctgcc 
ccttttgag gcaatcagc attatcaaa aggctcaat 
ttaacacag caaattcag gatttggtc ataattaaa 
atagaagaa cgtttgagg aaggaaata gtcccagat 
ttttggtcc cactttaag aaaaccgag aacgaggcg 
gcagttttt cacgagttc tacagtgtg gttaaattt 
ttgtttcac aattatgtg cagctcgat ttaattatt 
aacaagttg atactgttg agagaagca agcaatttc 
actaaacca gtgtacaat caaacttgc ctgcatgaa 
gctttaaaa ttaatcgtt agggccgtc ttgttatcc 
caactcccc attgatcat gaatggatg atagtaaat 
ttttacgaa acggcgctt aaaatgcag gacccggag 
gaagaaaat agcacaagt ggggagtgt gttgtgtgc 
atgcaagag gaattttcc tgttcttgt cgtgaaatg 
tttgaagaa actaataag aaattgggc aagatgact 
ttattggaa ccgttagtt ggccaggct ctcactgat 
ttaatttat agttacatt cagacacat atccagaaa 
atctgtaaa gacaatttt gataattcg cacattgag 
agtctagaa aagtggctt caaacggtt gttatttca 
tggttgaaa aaaatttac tttcataat tcggcagtc 
aaggccatg gatgagttt gacaaaaaa ctaatcaat 
tatttatac aacacttac accaaaata cgaatagat 
cagttattt aacatcata attgaatat cctgattct 
cttccggcc cttgacgac cttcgacta tgccttcca 
aaaaccgac ctaaaaccc cacttaact cgctcgttg 
caaaaagca attgaaaca agattatta catccagga 
gtaagcacg tttgacgtt ctgacggct tatgtggcc 
acaattcgg tcattgcgg attttggac ccctgtgga 
ctgcttcta gagacaatt actcaaccg gttcatcag 
tatttaagg agtcgggaa gacacaata aggtgtgtg 
gtgggtagt ttgactgaa gagggccca aatgattta 
gctgaagaa ctggtccgg ggcgaagcc gtgcaagtg 
gacgagaac atttcggtc gatgagggg gttgaagac 
tgggaaacg tggacccca gatccggta gatgtcgtc 
cccagcaaa tcttcggga cttccccgc acttctgac 
atcatctca atgcttgtc aacgtctac ggcagtaag 
gagctcttc gtcaacgaa taccgcacc ttattggcc 
gatcgtctt ttgtcgcaa tactcctgc gacacggag 
aaagaaatt cgttatctt gaactcctg aagttgcgc 
tttggtgac tcccaactc cacttttgc gaagttatg 
ttgaaagat atcgccgat tcgaagcga atcaatcaa 
catattcag caagacttg agttattca gacgatgat 
attcccatg tctgccatg atcctaagc gcacagttt 
tggccagcg ttcaaggac gagaagctt gaactccac 
gaaaaattg gtgaaacag atggagagt tacactggt 
gcttttgaa actttaaaa ggtagcaga accctgtgt 
tggaaaaat catttagga attgtggat cttgaaatt 
gaattggcg gataggact ttgaatttg tcagtttca 
ccggtgcat gcgacgatt ttgatgcac ttccaagat 
cagaatact tggtctttg gaagctttg agtaaagta 
atgcacgtt ccgtcgaca attttacgg cgcaaactg 
agtttctgg caatcacac gggatcata actgagccc 
gaacccgac gttttttat ctacaggag gaacaggaa 
aataacgaa aataatata caggatgat attgttgtt 
gaagatttt gaatcagag tcggcgatg gccagcgct 
caagaccaa cgcgaagag gaattacag actttctgg 
tcgtacatt ttgggtatg ttgatgaat ttggacact 
ttacctttg gagagaatc caccaaatg ttgaaaatg 
ttcgcattc caaggaccc acaattgag tgcaatttg 
caggaactg aaagtgttt ttagatagg aaagttaga 
gagcatcag ttgatttat tcaaacgga ctgtataga 
ttgcctaaa tag
    mRNA sequence
    • >TC002887 RA
      aaagtttgt cagtttttt acgttcagc aattattat 
ttttacaat gaataatca aaacctgga cttaaaaag 
tgcgtggat tacgattaa agaagcttt tccaatctt 
agaaagtcc ttcaaagat ccctgataa tgatttcga 
ctctgcccc ttttgaggc aatcagcat tatcaaaag 
gctcaattt aacacagca aattcagga tttggtcat 
aattaaaat agaagaacg tttgaggaa ggaaatagt 
cccagattt ttggtccca ctttaagaa aaccgagaa 
cgaggcggc agtttttca cgagttcta cagtgtggt 
taaattttt gtttcacaa ttatgtgca gctcgattt 
aattattaa caagttgat actgttgag agaagcaag 
caatttcac taaaccagt gtacaatca aacttgcct 
gcatgaagc tttaaaatt aatcgttag ggccgtctt 
gttatccca actccccat tgatcatga atggatgat 
agtaaattt ttacgaaac ggcgcttaa aatgcagga 
cccggagga agaaaatag cacaagtgg ggagtgtgt 
tgtgtgcat gcaagagga attttcctg ttcttgtcg 
tgaaatgtt tgaagaaac taataagaa attgggcaa 
gatgacttt attggaacc gttagttgg ccaggctct 
cactgattt aatttatag ttacattca gacacatat 
ccagaaaat ctgtaaaga caattttga taattcgca 
cattgagag tctagaaaa gtggcttca aacggttgt 
tatttcatg gttgaaaaa aatttactt tcataattc 
ggcagtcaa ggccatgga tgagtttga caaaaaact 
aatcaatta tttatacaa cacttacac caaaatacg 
aatagatca gttatttaa catcataat tgaatatcc 
tgattctct tccggccct tgacgacct tcgactatg 
ccttccaaa aaccgacct aaaacccca cttaactcg 
ctcgttgca aaaagcaat tgaaacaag attattaca 
tccaggagt aagcacgtt tgacgttct gacggctta 
tgtggccac aattcggtc attgcggat tttggaccc 
ctgtggact gcttctaga gacaattac tcaaccggt 
tcatcagta tttaaggag tcgggaaga cacaataag 
gtgtgtggt gggtagttt gactgaaga gggcccaaa 
tgatttagc tgaagaact ggtccgggg cgaagccgt 
gcaagtgga cgagaacat ttcggtcga tgagggggt 
tgaagactg ggaaacgtg gaccccaga tccggtaga 
tgtcgtccc cagcaaatc ttcgggact tccccgcac 
ttctgacat catctcaat gcttgtcaa cgtctacgg 
cagtaagga gctcttcgt caacgaata ccgcacctt 
attggccga tcgtctttt gtcgcaata ctcctgcga 
cacggagaa agaaattcg ttatcttga actcctgaa 
gttgcgctt tggtgactc ccaactcca cttttgcga 
agttatgtt gaaagatat cgccgattc gaagcgaat 
caatcaaca tattcagca agacttgag ttattcaga 
cgatgatat tcccatgtc tgccatgat cctaagcgc 
acagttttg gccagcgtt caaggacga gaagcttga 
actccacga aaaattggt gaaacagat ggagagtta 
cactggtgc ttttgaaac tttaaaagg tagcagaac 
cctgtgttg gaaaaatca tttaggaat tgtggatct 
tgaaattga attggcgga taggacttt gaatttgtc 
agtttcacc ggtgcatgc gacgatttt gatgcactt 
ccaagatca gaatacttg gtctttgga agctttgag 
taaagtaat gcacgttcc gtcgacaat tttacggcg 
caaactgag tttctggca atcacacgg gatcataac 
tgagcccga acccgacgt tttttatct acaggagga 
acaggaaaa taacgaaaa taatataca ggatgatat 
tgttgttga agattttga atcagagtc ggcgatggc 
cagcgctca agaccaacg cgaagagga attacagac 
tttctggtc gtacatttt gggtatgtt gatgaattt 
ggacacttt acctttgga gagaatcca ccaaatgtt 
gaaaatgtt cgcattcca aggacccac aattgagtg 
caatttgca ggaactgaa agtgttttt agataggaa 
agttagaga gcatcagtt gatttattc aaacggact 
gtatagatt gcctaaata gctttattt tatatttat 
aagtctttg cattttgct acctacgta ttcctatga 
tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY