Status
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Gene information
General information
TC002887 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7246024..7249441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002887
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00463

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber shape rounded - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 7

    wings not closed - Number of animals showing the phenotype: 7
    oblique dorsal thoracic muscles number increased - Number of animals showing the phenotype: 5

    longitudinal dorsal thoracic muscles number increased - Number of animals showing the phenotype: 5
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 5
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002887 RA
      MNNQNLDLK SAWITIKEA FPILESPSK IPDNDFDSA 
      PFEAISIIK RLNLTQQIQ DLVIIKIEE RLRKEIVPD 
      FWSHFKKTE NEAAVFHEF YSVVKFLFH NYVQLDLII 
      NKLILLREA SNFTKPVYN QTCLHEALK LIVRAVLLS 
      QLPIDHEWM IVNFYETAL KMQDPEEEN STSGECVVC 
      MQEEFSCSC REMFEETNK KLGKMTLLE PLVGQALTD 
      LIYSYIQTH IQKICKDNF DNSHIESLE KWLQTVVIS 
      WLKKIYFHN SAVKAMDEF DKKLINYLY NTYTKIRID 
      QLFNIIIEY PDSLPALDD LRLCLPKTD LKPHLTRSL 
      QKAIETRLL HPGVSTFDV LTAYVATIR SLRILDPCG 
      LLLETITQP VHQYLRSRE DTIRCVVGS LTEEGPNDL 
      AEELVRGEA VQVDENISV DEGVEDWET WTPDPVDVV 
      PSKSSGLPR TSDIISMLV NVYGSKELF VNEYRTLLA 
      DRLLSQYSC DTEKEIRYL ELLKLRFGD SQLHFCEVM 
      LKDIADSKR INQHIQQDL SYSDDDIPM SAMILSAQF 
      WPAFKDEKL ELHEKLVKQ MESYTGAFE TLKGSRTLC 
      WKNHLGIVD LEIELADRT LNLSVSPVH ATILMHFQD 
      QNTWSLEAL SKVMHVPST ILRRKLSFW QSHGIITEP 
      EPDVFYLQE EQENNENNI QDDIVVEDF ESESAMASA 
      QDQREEELQ TFWSYILGM LMNLDTLPL ERIHQMLKM 
      FAFQGPTIE CNLQELKVF LDRKVREHQ LIYSNGLYR 
      LPK
    CDS sequence
    • >TC002887 RA
      atgaataat caaaacctg gacttaaaa agtgcgtgg 
      attacgatt aaagaagct tttccaatc ttagaaagt 
      ccttcaaag atccctgat aatgatttc gactctgcc 
      ccttttgag gcaatcagc attatcaaa aggctcaat 
      ttaacacag caaattcag gatttggtc ataattaaa 
      atagaagaa cgtttgagg aaggaaata gtcccagat 
      ttttggtcc cactttaag aaaaccgag aacgaggcg 
      gcagttttt cacgagttc tacagtgtg gttaaattt 
      ttgtttcac aattatgtg cagctcgat ttaattatt 
      aacaagttg atactgttg agagaagca agcaatttc 
      actaaacca gtgtacaat caaacttgc ctgcatgaa 
      gctttaaaa ttaatcgtt agggccgtc ttgttatcc 
      caactcccc attgatcat gaatggatg atagtaaat 
      ttttacgaa acggcgctt aaaatgcag gacccggag 
      gaagaaaat agcacaagt ggggagtgt gttgtgtgc 
      atgcaagag gaattttcc tgttcttgt cgtgaaatg 
      tttgaagaa actaataag aaattgggc aagatgact 
      ttattggaa ccgttagtt ggccaggct ctcactgat 
      ttaatttat agttacatt cagacacat atccagaaa 
      atctgtaaa gacaatttt gataattcg cacattgag 
      agtctagaa aagtggctt caaacggtt gttatttca 
      tggttgaaa aaaatttac tttcataat tcggcagtc 
      aaggccatg gatgagttt gacaaaaaa ctaatcaat 
      tatttatac aacacttac accaaaata cgaatagat 
      cagttattt aacatcata attgaatat cctgattct 
      cttccggcc cttgacgac cttcgacta tgccttcca 
      aaaaccgac ctaaaaccc cacttaact cgctcgttg 
      caaaaagca attgaaaca agattatta catccagga 
      gtaagcacg tttgacgtt ctgacggct tatgtggcc 
      acaattcgg tcattgcgg attttggac ccctgtgga 
      ctgcttcta gagacaatt actcaaccg gttcatcag 
      tatttaagg agtcgggaa gacacaata aggtgtgtg 
      gtgggtagt ttgactgaa gagggccca aatgattta 
      gctgaagaa ctggtccgg ggcgaagcc gtgcaagtg 
      gacgagaac atttcggtc gatgagggg gttgaagac 
      tgggaaacg tggacccca gatccggta gatgtcgtc 
      cccagcaaa tcttcggga cttccccgc acttctgac 
      atcatctca atgcttgtc aacgtctac ggcagtaag 
      gagctcttc gtcaacgaa taccgcacc ttattggcc 
      gatcgtctt ttgtcgcaa tactcctgc gacacggag 
      aaagaaatt cgttatctt gaactcctg aagttgcgc 
      tttggtgac tcccaactc cacttttgc gaagttatg 
      ttgaaagat atcgccgat tcgaagcga atcaatcaa 
      catattcag caagacttg agttattca gacgatgat 
      attcccatg tctgccatg atcctaagc gcacagttt 
      tggccagcg ttcaaggac gagaagctt gaactccac 
      gaaaaattg gtgaaacag atggagagt tacactggt 
      gcttttgaa actttaaaa ggtagcaga accctgtgt 
      tggaaaaat catttagga attgtggat cttgaaatt 
      gaattggcg gataggact ttgaatttg tcagtttca 
      ccggtgcat gcgacgatt ttgatgcac ttccaagat 
      cagaatact tggtctttg gaagctttg agtaaagta 
      atgcacgtt ccgtcgaca attttacgg cgcaaactg 
      agtttctgg caatcacac gggatcata actgagccc 
      gaacccgac gttttttat ctacaggag gaacaggaa 
      aataacgaa aataatata caggatgat attgttgtt 
      gaagatttt gaatcagag tcggcgatg gccagcgct 
      caagaccaa cgcgaagag gaattacag actttctgg 
      tcgtacatt ttgggtatg ttgatgaat ttggacact 
      ttacctttg gagagaatc caccaaatg ttgaaaatg 
      ttcgcattc caaggaccc acaattgag tgcaatttg 
      caggaactg aaagtgttt ttagatagg aaagttaga 
      gagcatcag ttgatttat tcaaacgga ctgtataga 
      ttgcctaaa tag
    mRNA sequence
    • >TC002887 RA
      aaagtttgt cagtttttt acgttcagc aattattat 
      ttttacaat gaataatca aaacctgga cttaaaaag 
      tgcgtggat tacgattaa agaagcttt tccaatctt 
      agaaagtcc ttcaaagat ccctgataa tgatttcga 
      ctctgcccc ttttgaggc aatcagcat tatcaaaag 
      gctcaattt aacacagca aattcagga tttggtcat 
      aattaaaat agaagaacg tttgaggaa ggaaatagt 
      cccagattt ttggtccca ctttaagaa aaccgagaa 
      cgaggcggc agtttttca cgagttcta cagtgtggt 
      taaattttt gtttcacaa ttatgtgca gctcgattt 
      aattattaa caagttgat actgttgag agaagcaag 
      caatttcac taaaccagt gtacaatca aacttgcct 
      gcatgaagc tttaaaatt aatcgttag ggccgtctt 
      gttatccca actccccat tgatcatga atggatgat 
      agtaaattt ttacgaaac ggcgcttaa aatgcagga 
      cccggagga agaaaatag cacaagtgg ggagtgtgt 
      tgtgtgcat gcaagagga attttcctg ttcttgtcg 
      tgaaatgtt tgaagaaac taataagaa attgggcaa 
      gatgacttt attggaacc gttagttgg ccaggctct 
      cactgattt aatttatag ttacattca gacacatat 
      ccagaaaat ctgtaaaga caattttga taattcgca 
      cattgagag tctagaaaa gtggcttca aacggttgt 
      tatttcatg gttgaaaaa aatttactt tcataattc 
      ggcagtcaa ggccatgga tgagtttga caaaaaact 
      aatcaatta tttatacaa cacttacac caaaatacg 
      aatagatca gttatttaa catcataat tgaatatcc 
      tgattctct tccggccct tgacgacct tcgactatg 
      ccttccaaa aaccgacct aaaacccca cttaactcg 
      ctcgttgca aaaagcaat tgaaacaag attattaca 
      tccaggagt aagcacgtt tgacgttct gacggctta 
      tgtggccac aattcggtc attgcggat tttggaccc 
      ctgtggact gcttctaga gacaattac tcaaccggt 
      tcatcagta tttaaggag tcgggaaga cacaataag 
      gtgtgtggt gggtagttt gactgaaga gggcccaaa 
      tgatttagc tgaagaact ggtccgggg cgaagccgt 
      gcaagtgga cgagaacat ttcggtcga tgagggggt 
      tgaagactg ggaaacgtg gaccccaga tccggtaga 
      tgtcgtccc cagcaaatc ttcgggact tccccgcac 
      ttctgacat catctcaat gcttgtcaa cgtctacgg 
      cagtaagga gctcttcgt caacgaata ccgcacctt 
      attggccga tcgtctttt gtcgcaata ctcctgcga 
      cacggagaa agaaattcg ttatcttga actcctgaa 
      gttgcgctt tggtgactc ccaactcca cttttgcga 
      agttatgtt gaaagatat cgccgattc gaagcgaat 
      caatcaaca tattcagca agacttgag ttattcaga 
      cgatgatat tcccatgtc tgccatgat cctaagcgc 
      acagttttg gccagcgtt caaggacga gaagcttga 
      actccacga aaaattggt gaaacagat ggagagtta 
      cactggtgc ttttgaaac tttaaaagg tagcagaac 
      cctgtgttg gaaaaatca tttaggaat tgtggatct 
      tgaaattga attggcgga taggacttt gaatttgtc 
      agtttcacc ggtgcatgc gacgatttt gatgcactt 
      ccaagatca gaatacttg gtctttgga agctttgag 
      taaagtaat gcacgttcc gtcgacaat tttacggcg 
      caaactgag tttctggca atcacacgg gatcataac 
      tgagcccga acccgacgt tttttatct acaggagga 
      acaggaaaa taacgaaaa taatataca ggatgatat 
      tgttgttga agattttga atcagagtc ggcgatggc 
      cagcgctca agaccaacg cgaagagga attacagac 
      tttctggtc gtacatttt gggtatgtt gatgaattt 
      ggacacttt acctttgga gagaatcca ccaaatgtt 
      gaaaatgtt cgcattcca aggacccac aattgagtg 
      caatttgca ggaactgaa agtgttttt agataggaa 
      agttagaga gcatcagtt gatttattc aaacggact 
      gtatagatt gcctaaata gctttattt tatatttat 
      aagtctttg cattttgct acctacgta ttcctatga 
      tc
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