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Gene information
General information
TC002875 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 3049004..3052170
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002875
    iBeetle screen iB_00460
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber sometimes shape constricted - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002875 RA
      MGAPLEVLQ ECMKNTSCI RNVCILAHV DHGKTTIAD 
      SLLATNRLV SKRMAGLVR YLDDRLDEQ ERGITMKSS 
      AVSLINLVE DEDTKEEKP LLLNLIDTP GHIDFSSEV 
      GAALRVCDG ALVVVDLVE GVCVQTREA IKQAFTERC 
      KMILILNKI DKLIVELHK EVNDIFQSI LHAIEDCNA 
      IVAELYQYE YCNPDVDIE DTGLLFSPD AGNVIFASA 
      IDGWGFTLK QIASMFVNA IKSETVDSL NTKLWNFDA 
      YVDGKTNTV KLGAIEKGK TNLFVQLCI KTIFHIYST 
      IVIQMQRDK VGTIVQKLN ITNVTREMN HTDPKTQVK 
      AIMQAWSPL ADVILHQCM KIIPPPSNM HEDKIKYLL 
      DFNKFCENE FHKNCLEKL ITVFKSCSS DDSTPCTAY 
      VSKMFCVDN KNLSQNKPK VFIPKPRPK NTEPKQENG 
      EKTNHTEKE KNCEEEKPR DEFSIIALA RVFTGCLKT 
      GQEIYVLSP QYVPQEGKT SDTCAQLVK VKELYMLFG 
      RELVLVDEI TAGNVCGIG GLESAIVRT ATLSTTLQC 
      VAFIEHPSQ PPIVRNAIE PTNPKDLPI LRQGLRVLM 
      QSDSCVQVV IEESGEYVL LTAGDVHLA KCLEDLTTK 
      FAKIEINVS SPMVSLRET VTHGSNKSD LKKDLENSV 
      TVEVAQIRL TVVVVALPD VIANEIEKN YKLLHSIEE 
      HQQISGFEL FAKRASKPE DLKPPTLKM FKSDVTNVS 
      LKHVSEQLS SVFASCKGI WAKIENKVW SVGRMPDSI 
      NLLINGTSN YARNIYETL DEKDPRSCF DQFVVNAFN 
      SCCKAGPLC EEPLKNCVF LVKNFEVSH DESLDGTTK 
      TSVNIESAL SSAFREAFE KQQQRLMEP MFTTSIQVN 
      TNILGKVYS VVSKRHGKV LDAVGMDEQ EKSFLVKAQ 
      IPVVESTGF ANEMRKTTS GQAIPTLKF SHFEIIDGD 
      PFYEPVEDD EEEEDVNVE SAIRATKLR KAIRKRKGL 
      HVEEQVVVH GEKQRTLNK KK
    CDS sequence
    • >TC002875 RA
      atgggtgcc ccactcgaa gtcctccaa gaatgcatg 
      aaaaacaca tcgtgcata cgaaatgta tgcattttg 
      gcacacgtg gaccacggc aagaccaca atcgccgat 
      tcgctacta gccacgaat cgtttagta agcaaacgc 
      atggctggt ctggtcagg tatttagac gaccgactg 
      gacgagcaa gaacgtgga attaccatg aaaagtagc 
      gcagtgtct ttaataaac ttagtcgaa gatgaagac 
      acgaaagaa gaaaagccg cttttgttg aatttaatt 
      gatacaccc ggacacatc gatttctcc tccgaggtg 
      ggcgcagcc ctacgcgta tgcgacggg gccctagtc 
      gtggtcgac ttagtcgaa ggcgtgtgc gtacaaaca 
      cgcgaagcc atcaaacag gcgttcaca gaacgctgc 
      aaaatgata ctaattcta aacaaaata gataaatta 
      atcgtggag ttgcacaag gaagtgaac gacattttc 
      cagtcgatt ttgcacgcg attgaggac tgcaatgcg 
      atcgttgcg gaactgtac caatacgaa tattgcaat 
      cctgacgtc gatattgaa gacaccgga cttttgttt 
      tcaccggac gccggaaac gtgattttt gcaagtgcc 
      atcgatggg tggggcttc accctcaaa caaatagcc 
      tcgatgttt gtgaatgcc atcaaaagt gaaaccgta 
      gactcactg aatacgaaa ttatggaat ttcgatgct 
      tacgtcgac ggtaaaaca aacacagtt aaactagga 
      gcgatcgaa aagggcaaa actaattta tttgtgcaa 
      ctgtgcatc aaaacgata ttccacatc tactcaacc 
      atagtcatc caaatgcaa agggataaa gttggcacc 
      attgtccag aaactgaac attacgaac gtgacgcga 
      gaaatgaac cacacggac cccaaaacc caagtcaag 
      gccataatg caagcttgg agccccctt gccgatgtc 
      attttacac caatgtatg aaaattatc ccgccaccc 
      tcaaacatg cacgaagac aaaattaaa tatctcctc 
      gattttaac aaattttgc gaaaacgag tttcacaaa 
      aattgtctc gaaaaacta atcacggtg ttcaaatcg 
      tgttcctct gacgactcc acaccgtgt actgcatat 
      gtttcgaaa atgttttgc gtcgacaat aagaacttg 
      tcacaaaac aaacctaaa gtttttatc cccaagccg 
      agacctaaa aatactgaa cccaagcag gaaaatggc 
      gaaaaaaca aaccacact gaaaaggaa aaaaattgc 
      gaagaagag aaacctcgg gatgaattt tctataata 
      gcactggcg cgagttttc accggttgt ctcaaaacg 
      ggccaagaa atctacgtc ttgtcacct caatacgtc 
      ccccaagaa ggcaaaaca agtgatacg tgtgcccaa 
      ttagtgaaa gttaaagaa ctttatatg ctttttggt 
      cgtgagttg gtcctagtt gatgaaata acggctgga 
      aatgtgtgt ggtatcggg gggctggag tcggctata 
      gtccgcaca gcaacccta tcaacaact ctacagtgt 
      gtcgcattt attgaacac ccatcacag ccccctata 
      gtccgaaac gccatcgaa ccaaccaat ccgaaagac 
      cttccaatt ttaagacaa ggtttgcgc gtactaatg 
      caaagtgat tcttgcgtc caggtcgta atagaagaa 
      tcaggagag tatgtttta ctgacagca ggggacgta 
      caccttgct aaatgtctg gaagatttg acgacgaaa 
      tttgccaaa attgaaatc aacgtttcc agtccgatg 
      gtctcactg cgggaaact gtgacccac ggaagcaac 
      aaaagcgac ttaaagaaa gacctggag aattcggtc 
      acagtagaa gtggcccag attcgttta accgtagtt 
      gtggtcgct ttaccggac gttatagcc aacgaaatc 
      gaaaagaac tacaaactg ttgcactca atcgaagaa 
      caccaacaa atcagcgga ttcgaactg ttcgccaaa 
      cgtgcgtca aaacctgaa gacttgaaa cccccaact 
      ttaaaaatg ttcaaaagc gacgtcact aacgtctca 
      cttaaacat gtgagcgag caattaagc tcggttttc 
      gcttcgtgt aaaggcatt tgggccaaa atcgaaaat 
      aaggtctgg agcgtgggc cgcatgcct gacagtata 
      aatttacta atcaacggg accagtaat tacgcgcga 
      aatatttac gaaactttg gacgagaaa gacccgcgg 
      tcgtgtttc gaccaattt gtggtgaac gccttcaat 
      tcgtgctgc aaagctggc ccgctgtgt gaagaacca 
      ctgaaaaac tgtgttttc ctggtgaaa aactttgaa 
      gtcagtcat gacgagagc ttggacggg acgaccaaa 
      accagtgtt aatatcgaa tcggctctg agttcagcg 
      tttcgcgaa gcgttcgaa aaacaacag cagagactc 
      atggaaccg atgtttacg accagtatt caagtcaac 
      acaaatatt ttaggaaaa gtctactcg gttgtgagt 
      aaacggcac gggaaagtt ctggacgct gtcggaatg 
      gacgagcaa gagaaaagt tttctggtt aaggcccag 
      attccggtt gttgaaagt acgggtttt gctaacgaa 
      atgcgcaaa acgacttct gggcaagcg attcctact 
      ttgaagttt agtcatttt gagattatc gacggtgat 
      ccgttttac gagccggtg gaggacgac gaagaagag 
      gaagatgtg aatgtggag tcggctatc agggccacc 
      aaacttaga aaagcgata aggaaacgg aaagggttg 
      cacgtggag gaacaggtg gtcgtgcat ggagagaaa 
      caaaggacg ctcaataag aaaaaataa
    mRNA sequence
    • >TC002875 RA
      tgacttaac ctcaacttt tcacccttc attttccac 
      acatttcac cgttttaaa ccgcaattt cactcaaca 
      tgggtgccc cactcgaag tcctccaag aatgcatga 
      aaaacacat cgtgcatac gaaatgtat gcattttgg 
      cacacgtgg accacggca agaccacaa tcgccgatt 
      cgctactag ccacgaatc gtttagtaa gcaaacgca 
      tggctggtc tggtcaggt atttagacg accgactgg 
      acgagcaag aacgtggaa ttaccatga aaagtagcg 
      cagtgtctt taataaact tagtcgaag atgaagaca 
      cgaaagaag aaaagccgc ttttgttga atttaattg 
      atacacccg gacacatcg atttctcct ccgaggtgg 
      gcgcagccc tacgcgtat gcgacgggg ccctagtcg 
      tggtcgact tagtcgaag gcgtgtgcg tacaaacac 
      gcgaagcca tcaaacagg cgttcacag aacgctgca 
      aaatgatac taattctaa acaaaatag ataaattaa 
      tcgtggagt tgcacaagg aagtgaacg acattttcc 
      agtcgattt tgcacgcga ttgaggact gcaatgcga 
      tcgttgcgg aactgtacc aatacgaat attgcaatc 
      ctgacgtcg atattgaag acaccggac ttttgtttt 
      caccggacg ccggaaacg tgatttttg caagtgcca 
      tcgatgggt ggggcttca ccctcaaac aaatagcct 
      cgatgtttg tgaatgcca tcaaaagtg aaaccgtag 
      actcactga atacgaaat tatggaatt tcgatgctt 
      acgtcgacg gtaaaacaa acacagtta aactaggag 
      cgatcgaaa agggcaaaa ctaatttat ttgtgcaac 
      tgtgcatca aaacgatat tccacatct actcaacca 
      tagtcatcc aaatgcaaa gggataaag ttggcacca 
      ttgtccaga aactgaaca ttacgaacg tgacgcgag 
      aaatgaacc acacggacc ccaaaaccc aagtcaagg 
      ccataatgc aagcttgga gcccccttg ccgatgtca 
      ttttacacc aatgtatga aaattatcc cgccaccct 
      caaacatgc acgaagaca aaattaaat atctcctcg 
      attttaaca aattttgcg aaaacgagt ttcacaaaa 
      attgtctcg aaaaactaa tcacggtgt tcaaatcgt 
      gttcctctg acgactcca caccgtgta ctgcatatg 
      tttcgaaaa tgttttgcg tcgacaata agaacttgt 
      cacaaaaca aacctaaag tttttatcc ccaagccga 
      gacctaaaa atactgaac ccaagcagg aaaatggcg 
      aaaaaacaa accacactg aaaaggaaa aaaattgcg 
      aagaagaga aacctcggg atgaatttt ctataatag 
      cactggcgc gagttttca ccggttgtc tcaaaacgg 
      gccaagaaa tctacgtct tgtcacctc aatacgtcc 
      cccaagaag gcaaaacaa gtgatacgt gtgcccaat 
      tagtgaaag ttaaagaac tttatatgc tttttggtc 
      gtgagttgg tcctagttg atgaaataa cggctggaa 
      atgtgtgtg gtatcgggg ggctggagt cggctatag 
      tccgcacag caaccctat caacaactc tacagtgtg 
      tcgcattta ttgaacacc catcacagc cccctatag 
      tccgaaacg ccatcgaac caaccaatc cgaaagacc 
      ttccaattt taagacaag gtttgcgcg tactaatgc 
      aaagtgatt cttgcgtcc aggtcgtaa tagaagaat 
      caggagagt atgttttac tgacagcag gggacgtac 
      accttgcta aatgtctgg aagatttga cgacgaaat 
      ttgccaaaa ttgaaatca acgtttcca gtccgatgg 
      tctcactgc gggaaactg tgacccacg gaagcaaca 
      aaagcgact taaagaaag acctggaga attcggtca 
      cagtagaag tggcccaga ttcgtttaa ccgtagttg 
      tggtcgctt taccggacg ttatagcca acgaaatcg 
      aaaagaact acaaactgt tgcactcaa tcgaagaac 
      accaacaaa tcagcggat tcgaactgt tcgccaaac 
      gtgcgtcaa aacctgaag acttgaaac ccccaactt 
      taaaaatgt tcaaaagcg acgtcacta acgtctcac 
      ttaaacatg tgagcgagc aattaagct cggttttcg 
      cttcgtgta aaggcattt gggccaaaa tcgaaaata 
      aggtctgga gcgtgggcc gcatgcctg acagtataa 
      atttactaa tcaacggga ccagtaatt acgcgcgaa 
      atatttacg aaactttgg acgagaaag acccgcggt 
      cgtgtttcg accaatttg tggtgaacg ccttcaatt 
      cgtgctgca aagctggcc cgctgtgtg aagaaccac 
      tgaaaaact gtgttttcc tggtgaaaa actttgaag 
      tcagtcatg acgagagct tggacggga cgaccaaaa 
      ccagtgtta atatcgaat cggctctga gttcagcgt 
      ttcgcgaag cgttcgaaa aacaacagc agagactca 
      tggaaccga tgtttacga ccagtattc aagtcaaca 
      caaatattt taggaaaag tctactcgg ttgtgagta 
      aacggcacg ggaaagttc tggacgctg tcggaatgg 
      acgagcaag agaaaagtt ttctggtta aggcccaga 
      ttccggttg ttgaaagta cgggttttg ctaacgaaa 
      tgcgcaaaa cgacttctg ggcaagcga ttcctactt 
      tgaagttta gtcattttg agattatcg acggtgatc 
      cgttttacg agccggtgg aggacgacg aagaagagg 
      aagatgtga atgtggagt cggctatca gggccacca 
      aacttagaa aagcgataa ggaaacgga aagggttgc 
      acgtggagg aacaggtgg tcgtgcatg gagagaaac 
      aaaggacgc tcaataaga aaaaataaa agtgaaact 
      aacacgtga ataaatatt tttgtaaga t