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Gene information
General information
TC002864 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7863841..7872199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002864
    iBeetle screen iB_00459

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment: one male found
    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002864 RA
      MAKPEQVLH IEPQNELRF RGPFNAPVT 
      SYMKLTNPS DKKVLFKIK TTAPKKYCV 
      RPNSGVLEP NTSIEIAIS LQPFLFDPA 
      EKNKHKFMV QTLFAPDGD VNLEQLWKE 
      IGPEQLMDS KLKCVFEMP VEQNESQEE 
      ATKASPKAA VAAVPNNAI DPELQKAAE 
      EVQQLREEE SSLRQENLR LKEEILQLK 
      LAAGARVTQ TNRYAPPAQ QQNIPVIYM 
      AVAVILGFL GIVLGKFVL
    CDS sequence
    • >TC002864 RA
      atggcgaag cccgaacag gtcctacac atcgaaccc 
      cagaacgag ctgcgattt cgagggccc ttcaacgct 
      ccggtgacc tcctacatg aaactaacg aacccatca 
      gacaaaaaa gtcctgttc aaaatcaaa acgacagcg 
      ccaaaaaag tactgcgta cgccccaac tcgggtgtc 
      ctagagccc aacacctca atagagata gcaataagt 
      ctccagccg ttcctgttc gacccagcg gaaaagaac 
      aaacacaaa tttatggta cagacgtta ttcgcgcca 
      gacggtgac gtgaatctc gaacagtta tggaaagaa 
      atcggcccc gaacagctg atggactcc aagttgaaa 
      tgcgttttc gaaatgcca gtcgaacag aacgaaagc 
      caagaggaa gccactaaa gctagtccg aaagctgcc 
      gtcgcggcg gtacccaat aacgcaata gatccggaa 
      ctgcagaag gcggccgag gaggttcaa caacttagg 
      gaagaagaa agttcgttg aggcaggag aacctgcga 
      cttaaagag gaaattctt caattaaag ctggcagca 
      ggagcgcga gtgacccaa accaaccga tacgcacct 
      ccagcgcag cagcagaac atcccagta atatacatg 
      gctgtggct gtaattttg ggcttctta ggcatcgta 
      ttgggcaag tttgtcctc tga
    mRNA sequence
    • >TC002864 RA
      cgcgcgtgc gcgttagct gtcagaaag tgcagcata 
      accgggatc tgtcaccga aagtgtgta aaaaaattg 
      cgtttgtag ttgtttttg gtggtttca tccagtgat 
      aggttaaaa caatggcga agcccgaac aggtcctac 
      acatcgaac cccagaacg agctgcgat ttcgagggc 
      ccttcaacg ctccggtga cctcctaca tgaaactaa 
      cgaacccat cagacaaaa aagtcctgt tcaaaatca 
      aaacgacag cgccaaaaa agtactgcg tacgcccca 
      actcgggtg tcctagagc ccaacacct caatagaga 
      tagcaataa gtctccagc cgttcctgt tcgacccag 
      cggaaaaga acaaacaca aatttatgg tacagacgt 
      tattcgcgc cagacggtg acgtgaatc tcgaacagt 
      tatggaaag aaatcggcc ccgaacagc tgatggact 
      ccaagttga aatgcgttt tcgaaatgc cagtcgaac 
      agaacgaaa gccaagagg aagccacta aagctagtc 
      cgaaagctg ccgtcgcgg cggtaccca ataacgcaa 
      tagatccgg aactgcaga aggcggccg aggaggttc 
      aacaactta gggaagaag aaagttcgt tgaggcagg 
      agaacctgc gacttaaag aggaaattc ttcaattaa 
      agctggcag caggagcgc gagtgaccc aaaccaacc 
      gatacgcac ctccagcgc agcagcaga acatcccag 
      taatataca tggctgtgg ctgtaattt tgggcttct 
      taggcatcg tattgggca agtttgtcc tctgagacc 
      ctccttaga gacttagcg tggtgttac tcagattac 
      catgcccaa actccaata tattgtata tttattaca 
      gttacttca ttattatct actgtaaaa taaaatttc 
      ttgtgcata cttaacggg tgtgctgtt ttggttgca 
      tctttaggc caatcggtt cgttgccaa tcttaacat 
      cgtcaaaaa tggtttagt ttagatgga ggagggtgt 
      gttgaggtt ggatggaag actggttgg tcgtttttt 
      tacagagtg tttattata aacgtagct aaacgaatt 
      taaatgtcg gaattgttt tatttggaa catggatgt 
      tttactttt ttttgtctc agcagtcgc agattagaa 
      ctatatata caatatatg gcaagttta cttactagc 
      catcttttg gacgtttca tgccttaag ttttcattt 
      cgtatacag tgacagtcc aaatgaact tgttgctaa 
      atttggaat acattttaa ggtgaatgt tgttttcat 
      acacgcact gactgtaca taactttga actttctct 
      gtatactct agtgtgtca agcaacctt ccggcactg 
      ttgttttaa ataaaactc accattgtg ttagatgta 
      tttattttg tttttaatt taatgtttt aatatagag 
      gaaagttgc ttgactctt gcatttttt taatttctc 
      ttagtattt gtggtaata gttgagtca ttttgtaat 
      tgttagtta gatcgagca cttgccaag gttcattga 
      gagagttca ctttagaat cacgtaatt cttgcattt 
      gggttcaga acatcctac tttagacta ggtccactt 
      catattaat gtggtgctg tcgtattta catttttat 
      tcatccttc gatttgata ttacattaa tttgacatg 
      aaagaggtt tttacatgt gtctgtgag ttttgttaa 
      cttatagat ttagggcaa ataaacatc taaaattaa 
      ttgtagtaa gaacttggc ttggtgcga ttgttcata 
      tgtattata ttttaacac gttgtacta ttttatgct 
      agcctggta agtgtaagt aacttcagt gaataaact 
      atacttcat tacctactc atacacttt ttgtaagcc 
      gttatctta ccatacaac taaatatat taattgaac 
      cgatgtaaa acatagcaa attataact gacatgttg 
      cattaacac tcgcctttt attctccaa aacgtacgt 
      cacctaaaa ctaattcag aattatttc atcgccact 
      tctgactca ctctttgta ggccagact acaactgca 
      ttgcacaat acagttatt catttcac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY