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Gene information
General information
TC002857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8043281..8046539
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002857
    iBeetle screen iB_00457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4
    vitellarium not present - Number of animals showing the phenotype: 4
    tropharium shape irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002857 RA
      MGETKPVEN TEKADSKKA AKKEAKKAE KAAKKAEHK 
      AASGQSQSV VADENDVSV GKYGNLPLI RSDSTNTNK 
      DRKFIYVKE LNKSLAGND VWVRARLHT VRARGKQCF 
      IVLRQREFT VQLLLNVSE TVSKSMVKF ASNITKESI 
      IDVEAKVVQ VPTPIAACS QQDVELLAS QIWVVSASA 
      PQLPLQIED ASRPEKGDE AEGLNIRVN QDTRLDNRV 
      LDLRTPTNQ AIFRLEAGV CRLFREVLT RKGFTEIHT 
      PKIIPAASE GGANVFTVS YFKGSAYLA QSPQLYKQM 
      AIAADFDKV FTIGAVFRA EDSNTHRHL TEFTGLDLE 
      MAFNYHYHE VMLLIGNSF TEIFKGLRD QYQTEIQII 
      GQQFSVEPF TFLDPPLVL HFDEGRRLL GEAGVEVGP 
      EEDLSTPAE KLLGRLVKA KYDTDFYIL DKYPLAVRP 
      FYTMPDPET PRSSNSYDM FMRGEEILS GAQRIHDPQ 
      LLTERAKHH GIDLKKIAS YIDAFKYGC PPHAGGGIG 
      LERVVMLYL GLDNIRKTC MFPRDPKRI TP
    CDS sequence
    • >TC002857 RA
      atgggcgaa accaagccg gtcgaaaac actgaaaag 
      gctgatagt aaaaaggca gccaagaaa gaggccaaa 
      aaggccgaa aaagcggca aaaaaagct gaacacaaa 
      gccgccagt ggacagtca cagtctgtt gttgctgat 
      gaaaatgat gtatctgtt ggaaaatat gggaatttg 
      ccgttaatc cggtccgat agcactaac actaataag 
      gaccgcaaa tttatttat gtcaaagag ttaaataaa 
      tctcttgcc ggtaatgat gtttgggtg agggctagg 
      cttcacact gtccgtgcc aggggaaag cagtgtttt 
      atcgtcctc agacaaagg gagtttacc gtccagtta 
      cttctgaat gtctctgag acagtttcg aaatctatg 
      gtcaagttt gcaagcaac atcacgaaa gaaagcatc 
      attgatgtg gaggccaaa gtagtgcaa gttccgact 
      ccaatcgct gcctgttcc cagcaagac gtggaattg 
      ttggcgtcg caaatttgg gttgtctcg gcctcagcc 
      ccccaactg cctttacag attgaggat gcaagcagg 
      ccggaaaag ggcgacgag gcagaaggg ctcaatatt 
      cgggtcaac caagacact agattggac aaccgggtc 
      ctagattta cgcacaccg accaatcag gcgattttc 
      aggctagag gccggcgtt tgccgcctt ttcagagaa 
      gttttaaca aggaaagga tttacggag attcacacg 
      ccgaaaata attcctgcc gcgtctgaa ggcggcgcc 
      aatgtcttc acagtcagt tatttcaaa gggtcggca 
      tatttggct caaagtccg caactgtac aaacaaatg 
      gcaatagcg gccgatttc gataaggtt tttacgatt 
      ggggccgtt ttccgcgct gaggactcc aacacccac 
      cgccacttg accgaattc acaggcctt gacttagaa 
      atggccttc aactatcac tatcacgaa gtgatgctt 
      ttgattggg aattcgttc actgaaatt ttcaaagga 
      ctgcgtgac caataccag actgaaatt cagataatc 
      ggtcaacaa ttctcagtc gagcctttt actttcttg 
      gaccctccc ctagtttta cactttgac gaagggcgc 
      agacttttg ggggaagcc ggggttgaa gtggggccc 
      gaagaggat ttgagtaca cctgctgaa aagttgcta 
      gggcggctg gtgaaagcc aaatacgac accgatttt 
      tacatcttg gacaagtat ccattggct gtgaggccg 
      ttctacacc atgccggac ccagagaca cccaggtcc 
      tcaaattcg tacgatatg tttatgagg ggtgaggag 
      atcctgagc ggggctcag aggattcac gatccgcag 
      ttgttgacc gaacgggcc aaacatcat ggaattgat 
      ttaaagaaa attgcttcg tacattgat gctttcaag 
      tacgggtgt ccgccccac gctgggggc ggcatcggc 
      ttggaacgc gtggtgatg ctttatttg gggttggat 
      aacatcagg aagacttgc atgttcccg cgtgacccc 
      aaacgaatc acgccgtaa
    mRNA sequence
    • >TC002857 RA
      tatcttcac cgatattaa aaatttagt tgaaaaatc 
      gctgccaac atgatgtaa ttgtcagat ggttgtcaa 
      gtttgacac gtgagagaa gtgctctag gagtgttct 
      tttcaaagc aaaaatggg cgaaaccaa gccggtcga 
      aaacactga aaaggctga tagtaaaaa ggcagccaa 
      gaaagaggc caaaaaggc cgaaaaagc ggcaaaaaa 
      agctgaaca caaagccgc cagtggaca gtcacagtc 
      tgttgttgc tgatgaaaa tgatgtatc tgttggaaa 
      atatgggaa tttgccgtt aatccggtc cgatagcac 
      taacactaa taaggaccg caaatttat ttatgtcaa 
      agagttaaa taaatctct tgccggtaa tgatgtttg 
      ggtgagggc taggcttca cactgtccg tgccagggg 
      aaagcagtg ttttatcgt cctcagaca aagggagtt 
      taccgtcca gttacttct gaatgtctc tgagacagt 
      ttcgaaatc tatggtcaa gtttgcaag caacatcac 
      gaaagaaag catcattga tgtggaggc caaagtagt 
      gcaagttcc gactccaat cgctgcctg ttcccagca 
      agacgtgga attgttggc gtcgcaaat ttgggttgt 
      ctcggcctc agcccccca actgccttt acagattga 
      ggatgcaag caggccgga aaagggcga cgaggcaga 
      agggctcaa tattcgggt caaccaaga cactagatt 
      ggacaaccg ggtcctaga tttacgcac accgaccaa 
      tcaggcgat tttcaggct agaggccgg cgtttgccg 
      ccttttcag agaagtttt aacaaggaa aggatttac 
      ggagattca cacgccgaa aataattcc tgccgcgtc 
      tgaaggcgg cgccaatgt cttcacagt cagttattt 
      caaagggtc ggcatattt ggctcaaag tccgcaact 
      gtacaaaca aatggcaat agcggccga tttcgataa 
      ggtttttac gattggggc cgttttccg cgctgagga 
      ctccaacac ccaccgcca cttgaccga attcacagg 
      ccttgactt agaaatggc cttcaacta tcactatca 
      cgaagtgat gcttttgat tgggaattc gttcactga 
      aattttcaa aggactgcg tgaccaata ccagactga 
      aattcagat aatcggtca acaattctc agtcgagcc 
      ttttacttt cttggaccc tcccctagt tttacactt 
      tgacgaagg gcgcagact tttggggga agccggggt 
      tgaagtggg gcccgaaga ggatttgag tacacctgc 
      tgaaaagtt gctagggcg gctggtgaa agccaaata 
      cgacaccga tttttacat cttggacaa gtatccatt 
      ggctgtgag gccgttcta caccatgcc ggacccaga 
      gacacccag gtcctcaaa ttcgtacga tatgtttat 
      gaggggtga ggagatcct gagcggggc tcagaggat 
      tcacgatcc gcagttgtt gaccgaacg ggccaaaca 
      tcatggaat tgatttaaa gaaaattgc ttcgtacat 
      tgatgcttt caagtacgg gtgtccgcc ccacgctgg 
      gggcggcat cggcttgga acgcgtggt gatgcttta 
      tttggggtt ggataacat caggaagac ttgcatgtt 
      cccgcgtga ccccaaacg aatcacgcc gtaaattga 
      tacaagact aatgtttta taagaataa ctaataaac 
      ttattgttt caacttgta tttacaaaa attacaata 
      aaattgaat tttacttaa gttttttta ttatgtgaa 
      gcctccagg gcttccaaa cacccatcc atgtttact 
      ttctgaacc gactctccc ttttccgcc aagatttct 
      tctatattc cttttacca tttaaatca gtgaaccag 
      tttaaatta tcagcaatc aaatggttg ttgttgcaa 
      ccggagcat ttcacgata acgacacct ttctggtaa 
      gccaccttg cttatgaat tttgtgttc cgagcacta 
      cacacttta cacgtaaaa cccaacatt aattttccc 
      tcgagcttt gcaaggggt gtggaagtg gatgattca 
      tcttctgtt tcattttga actttattt atactgtgg 
      tttagccaa gaactgtga aaatgtcgt gctgttatc 
      acttgtgag gtgtcccca aattttttt attgtttgg 
      gctttgaca acaggtgat ttggttacg aatcggagt 
      aaagtggta agacgcgac ctccaggcc attattttc 
      gtacaaaaa ccaacgaaa tgtgcacta aacacgact 
      ttttgctac ctaaccttt actataaat gaggttacg 
      tcatgttat tacctccga ttgttaaat attaatcca 
      ttagtgagt ttattgtcc cactatctt tgctatctg 
      tttaattta ttagtaagt gactgttag cattatcat 
      aatttccaa aaaaaaaat atccgcgac caataaatt 
      tgaaacaca tat
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