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Gene information
General information
TC002851 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 3585534..3591781
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002851
    iBeetle screen iB_00455
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002851 RA
      MTVTESSEH DGEKDENSA EAPSEDDKN VLTSYIQEV 
      EERFVCKQE LRSANQNAA NTRPSESHF SKLDSSLKK 
      NTAFVKKIK NFSANQVDT YLKDMTGLN LSKYISEIA 
      AAIVDAKLK MTDVGAAVK MCSVLHQTY ADFSQHLFE 
      NWQKTLAFK VGEKIPNPS KLRVDLRFY AELVQAGVF 
      SNKNAFTLL GTVITTLVN MDKDEHYNC SIILSFCKH 
      CGEDYAGLV PRKMRELSE KFDIPIPKS TFLPPEKQQ 
      NVRTLLRDY YVSLSKHLV KDHQEIQNF EKQNMRILQ 
      TKGELSQER KDKLEALQT SYDKLLAST QSFAEILDE 
      DMPTLKNQT LMKNDESMI VTGAGPDLD EAALANIEN 
      VWCDIETQR FYCELPELQ AFLPTSYIN KQAPPPEES 
      VTEEVLDSE IPTEELEDD EKAEEAPVA AEDEPEDSA 
      TTNASNKIL LEAFFNNLP NCVNREMID NAAIDFLVT 
      LNNKHNRKK LVRALFGVN RTRLDLLPF YARFVAILR 
      PALPEVGNE LCQMLRQDF KYHVRKKDQ INIESKIKV 
      VRFIGELVK FKLYSKIEA LYCLKVLLY DFSHHHIEM 
      ACNLLEVCG RFLYCSPDS HQRTRVYLE QMMRKKAVM 
      ALDSRYVTQ IENAYYYVN PPEVTITKK ERPIMHQFI 
      RKLLFQDLQ KNNTDKIMR LIRKLDWGN EDVSAYAIK 
      CLSSAHNVK YFNIRCLAN LLAGLVTYQ EEVGTRVVD 
      SVLEDIRLG MEVNLPKYN QRRVAQVKY LGELYNYRM 
      VESADIFKV LYSLITFGV SMDPNIPSP LDPPNHLFR 
      IRLACVLLE TCGTYFSSG SSKRRLDYY LIFLQSYYW 
      FKRDLWKDA FPPTLEHIF KDTLTTLRP KIKLCQSYE 
      ETQTELNNI RVTLGFDKI TGDLNENKV EEDGLETIA 
      ETDTEEQDE VSEVTAPIT DDTATEDET VDHTQGSDD 
      EGEPDVSEA EQEQVSESL AIPSGPRKV ECPEDEDFL 
      NALDKMVSE NIQERMREP VKAGNVDIS VPVVLKSNK 
      KTYEQLQDA PETEPNKIG FVLMVRKGN KQQYKNFEA 
      DINSELAQN LRDQELAQK EEKERVKRL TLNITERFE 
      EEDYQDMMQ QQNRPVTQN LNRERKKYQ HPKGAPDAD 
      LIFGPKRVR
    CDS sequence
    • >TC002851 RA
      atgaccgtg accgaatcc tcggaacac gacggtgag 
      aaagacgag aacagtgcc gaggccccc agcgaagac 
      gacaaaaac gtcctaacc tcttacatc caagaggtg 
      gaagagagg tttgtttgc aagcaagag ctgcgaagt 
      gccaaccaa aatgcggca aatacgcgc cccagcgag 
      tcccacttc agcaagctg gactccagc ctcaagaag 
      aacacggcg tttgttaag aaaatcaaa aacttctct 
      gccaaccaa gtggacaca tacttgaaa gacatgacg 
      gggctgaac ctgtccaag tacatatcg gagattgcg 
      gctgcgata gtcgatgct aaattgaaa atgaccgat 
      gtgggcgca gccgtaaag atgtgcagt gttttgcac 
      cagacttac gctgatttc tcgcaacac ttgtttgag 
      aactggcag aaaaccctg gcctttaaa gtgggtgag 
      aagattcca aatccgagt aaactgagg gtggattta 
      aggttttat gccgagttg gtgcaggct ggggtgttt 
      agcaataaa aacgcgttc actttgctg gggaccgtg 
      atcaccact ttggtcaac atggacaag gacgaacac 
      tacaattgc tcaataatc ctcagcttt tgcaagcac 
      tgcggcgaa gattacgca ggtctggtc cctcgcaag 
      atgcgggaa ttgtccgaa aaattcgac atccccatc 
      ccgaagagc actttcctg ccgcccgag aagcagcaa 
      aacgtgcgc accttgctg agggattac tacgtttcg 
      ctcagtaag catctcgtc aaagaccac caagagatc 
      caaaacttc gagaagcag aacatgagg atactccaa 
      accaaaggc gagttgagt caagaacga aaagacaag 
      ctcgaggcg cttcaaaca tcgtacgat aaattatta 
      gcttcgaca caaagtttt gcggaaatt ttagacgaa 
      gatatgcca actttgaaa aatcagact ctcatgaaa 
      aatgatgag agtatgata gtgacaggt gcggggccc 
      gatttggac gaagctgcg ttggctaat atcgagaat 
      gtttggtgc gatattgaa acgcagagg ttttactgc 
      gagttgccg gaattgcaa gcttttttg ccgacttct 
      tatataaat aaacaggcg cctccgccc gaggagtcg 
      gtcactgag gaggtgctg gattcggaa ataccaact 
      gaagaactg gaagatgac gaaaaagcc gaggaggcg 
      cccgtggca gcggaagat gagcccgaa gactctgcc 
      acaaccaac gccagcaat aaaatcctc ctcgaggca 
      tttttcaac aacttgccc aactgcgtg aaccgcgaa 
      atgatcgac aacgcagcc atcgatttc ctcgtaacc 
      ctcaacaac aaacacaac cgcaagaag ctggtccgt 
      gccctgttc ggggtaaac cgcacccgt ctcgacctc 
      ctcccgttc tacgcccgc ttcgtggcc atcctccgt 
      ccagccctt cccgaagtg ggcaacgaa ctgtgccaa 
      atgctgcgg caagacttc aaataccac gtacgcaag 
      aaagaccaa attaacatc gaatccaaa ataaaagtc 
      gtacgattt atcggcgaa ctcgtcaaa ttcaagctc 
      tattcaaaa atagaagct ctctattgt ctcaaagtt 
      ctgctgtac gatttctcg caccatcac atcgaaatg 
      gcctgcaat ctgttggaa gtttgcggc cggtttttg 
      tactgcagt cccgactcg catcaaagg acgagggtg 
      tatttggag caaatgatg aggaaaaag gccgttatg 
      gcgttggat tcgagatat gtgacgcaa attgaaaac 
      gcctattat tacgtcaat ccgcccgaa gtaacaatc 
      acgaagaag gagaggccg ataatgcac cagtttatc 
      cggaaattg ctctttcaa gacttgcag aagaataac 
      actgataag attatgagg ctgattagg aagctggac 
      tggggcaac gaggacgtg tccgcttat gcaataaag 
      tgtctaagc agcgcccac aacgtgaag tatttcaat 
      atcaggtgt ctcgcaaat ttgcttgcg ggcttggtg 
      acctaccag gaagaggtt ggcactcgt gtagtggac 
      agtgtttta gaagacatt cggttaggt atggaagta 
      aatcttccc aaatataac cagagacgt gtagctcaa 
      gtaaaatat cttggggag ctgtataac tacagaatg 
      gttgaaagc gctgacatt ttcaaggtt ttgtattct 
      ttgataaca tttggagtt tcaatggat ccaaacata 
      ccgtcgcca ctcgatcca ccaaatcac cttttccga 
      ataagactg gcttgtgtt ttacttgaa acatgcggc 
      acgtatttc agcagcggt agcagtaag aggagacta 
      gactattat ttaatattt ctgcaatcg tattattgg 
      ttcaagcgc gacttgtgg aaagatgca tttcctcca 
      acactggag cacattttt aaagacact ctcacgacc 
      ctgagacca aaaattaaa ttgtgtcaa agctacgaa 
      gaaacacaa accgagtta aataatatt cgcgtcact 
      ttgggcttt gataaaatc actggagat ttgaacgaa 
      aataaagtt gaggaagac ggcctggaa acgatcgct 
      gaaaccgac actgaagaa caggacgaa gtgtccgaa 
      gtcacggca cctattact gatgatacc gccactgag 
      gacgaaacg gtggaccac acacaaggc tcggacgat 
      gaaggggaa cccgatgtt agcgaagct gagcaagaa 
      caagtgtcc gaatcgctg gcaattccc agtggcccc 
      cgcaaggtc gagtgcccc gaggacgag gacttttta 
      aacgctttg gataaaatg gtttcggag aacatccaa 
      gagcggatg cgcgagccg gtgaaggcc ggaaacgtg 
      gacatttcc gttccggtt gtcctcaaa tcgaacaaa 
      aagacctac gagcagttg caggacgcc cctgaaacg 
      gaacccaac aaaatcggg ttcgtgcta atggtccgc 
      aaaggaaac aagcagcaa tataaaaat ttcgaggct 
      gatatcaac tccgaactc gctcagaat ctgagggac 
      caggagttg gctcagaag gaggagaag gaacgagtg 
      aagcgcttg acgctgaac atcacggaa cggtttgaa 
      gaggaggat tatcaagac atgatgcag cagcagaac 
      aggcccgtg acccaaaac ttgaaccgc gaacgcaag 
      aaataccag caccccaag ggggcgccc gacgccgac 
      ctgattttt ggacctaag agagttaga taa
    mRNA sequence
    • >TC002851 RA
      tgtacattt gtccgggtt aaatccgaa ttattgtct 
      ttaattttg atgatgtaa acactttcc gttcgcttg 
      gaattaacg ataagataa cacgaaagt tgattgtgt 
      aacttgcat tggcaacac ttatatcta ttgacggtt 
      gggaatttt cgctttttc cctaaattg ttttctaat 
      aataatcat tttaacaag ttttttgca aataaagcc 
      cgttttgcg acatgaccg tgaccgaat cctcggaac 
      acgacggtg agaaagacg agaacagtg ccgaggccc 
      ccagcgaag acgacaaaa acgtcctaa cctcttaca 
      tccaagagg tggaagaga ggtttgttt gcaagcaag 
      agctgcgaa gtgccaacc aaaatgcgg caaatacgc 
      gccccagcg agtcccact tcagcaagc tggactcca 
      gcctcaaga agaacacgg cgtttgtta agaaaatca 
      aaaacttct ctgccaacc aagtggaca catacttga 
      aagacatga cggggctga acctgtcca agtacatat 
      cggagattg cggctgcga tagtcgatg ctaaattga 
      aaatgaccg atgtgggcg cagccgtaa agatgtgca 
      gtgttttgc accagactt acgctgatt tctcgcaac 
      acttgtttg agaactggc agaaaaccc tggccttta 
      aagtgggtg agaagattc caaatccga gtaaactga 
      gggtggatt taaggtttt atgccgagt tggtgcagg 
      ctggggtgt ttagcaata aaaacgcgt tcactttgc 
      tggggaccg tgatcacca ctttggtca acatggaca 
      aggacgaac actacaatt gctcaataa tcctcagct 
      tttgcaagc actgcggcg aagattacg caggtctgg 
      tccctcgca agatgcggg aattgtccg aaaaattcg 
      acatcccca tcccgaaga gcactttcc tgccgcccg 
      agaagcagc aaaacgtgc gcaccttgc tgagggatt 
      actacgttt cgctcagta agcatctcg tcaaagacc 
      accaagaga tccaaaact tcgagaagc agaacatga 
      ggatactcc aaaccaaag gcgagttga gtcaagaac 
      gaaaagaca agctcgagg cgcttcaaa catcgtacg 
      ataaattat tagcttcga cacaaagtt ttgcggaaa 
      ttttagacg aagatatgc caactttga aaaatcaga 
      ctctcatga aaaatgatg agagtatga tagtgacag 
      gtgcggggc ccgatttgg acgaagctg cgttggcta 
      atatcgaga atgtttggt gcgatattg aaacgcaga 
      ggttttact gcgagttgc cggaattgc aagcttttt 
      tgccgactt cttatataa ataaacagg cgcctccgc 
      ccgaggagt cggtcactg aggaggtgc tggattcgg 
      aaataccaa ctgaagaac tggaagatg acgaaaaag 
      ccgaggagg cgcccgtgg cagcggaag atgagcccg 
      aagactctg ccacaacca acgccagca ataaaatcc 
      tcctcgagg catttttca acaacttgc ccaactgcg 
      tgaaccgcg aaatgatcg acaacgcag ccatcgatt 
      tcctcgtaa ccctcaaca acaaacaca accgcaaga 
      agctggtcc gtgccctgt tcggggtaa accgcaccc 
      gtctcgacc tcctcccgt tctacgccc gcttcgtgg 
      ccatcctcc gtccagccc ttcccgaag tgggcaacg 
      aactgtgcc aaatgctgc ggcaagact tcaaatacc 
      acgtacgca agaaagacc aaattaaca tcgaatcca 
      aaataaaag tcgtacgat ttatcggcg aactcgtca 
      aattcaagc tctattcaa aaatagaag ctctctatt 
      gtctcaaag ttctgctgt acgatttct cgcaccatc 
      acatcgaaa tggcctgca atctgttgg aagtttgcg 
      gccggtttt tgtactgca gtcccgact cgcatcaaa 
      ggacgaggg tgtatttgg agcaaatga tgaggaaaa 
      aggccgtta tggcgttgg attcgagat atgtgacgc 
      aaattgaaa acgcctatt attacgtca atccgcccg 
      aagtaacaa tcacgaaga aggagaggc cgataatgc 
      accagttta tccggaaat tgctctttc aagacttgc 
      agaagaata acactgata agattatga ggctgatta 
      ggaagctgg actggggca acgaggacg tgtccgctt 
      atgcaataa agtgtctaa gcagcgccc acaacgtga 
      agtatttca atatcaggt gtctcgcaa atttgcttg 
      cgggcttgg tgacctacc aggaagagg ttggcactc 
      gtgtagtgg acagtgttt tagaagaca ttcggttag 
      gtatggaag taaatcttc ccaaatata accagagac 
      gtgtagctc aagtaaaat atcttgggg agctgtata 
      actacagaa tggttgaaa gcgctgaca ttttcaagg 
      ttttgtatt ctttgataa catttggag tttcaatgg 
      atccaaaca taccgtcgc cactcgatc caccaaatc 
      accttttcc gaataagac tggcttgtg ttttacttg 
      aaacatgcg gcacgtatt tcagcagcg gtagcagta 
      agaggagac tagactatt atttaatat ttctgcaat 
      cgtattatt ggttcaagc gcgacttgt ggaaagatg 
      catttcctc caacactgg agcacattt ttaaagaca 
      ctctcacga ccctgagac caaaaatta aattgtgtc 
      aaagctacg aagaaacac aaaccgagt taaataata 
      ttcgcgtca ctttgggct ttgataaaa tcactggag 
      atttgaacg aaaataaag ttgaggaag acggcctgg 
      aaacgatcg ctgaaaccg acactgaag aacaggacg 
      aagtgtccg aagtcacgg cacctatta ctgatgata 
      ccgccactg aggacgaaa cggtggacc acacacaag 
      gctcggacg atgaagggg aacccgatg ttagcgaag 
      ctgagcaag aacaagtgt ccgaatcgc tggcaattc 
      ccagtggcc cccgcaagg tcgagtgcc ccgaggacg 
      aggactttt taaacgctt tggataaaa tggtttcgg 
      agaacatcc aagagcgga tgcgcgagc cggtgaagg 
      ccggaaacg tggacattt ccgttccgg ttgtcctca 
      aatcgaaca aaaagacct acgagcagt tgcaggacg 
      cccctgaaa cggaaccca acaaaatcg ggttcgtgc 
      taatggtcc gcaaaggaa acaagcagc aatataaaa 
      atttcgagg ctgatatca actccgaac tcgctcaga 
      atctgaggg accaggagt tggctcaga aggaggaga 
      aggaacgag tgaagcgct tgacgctga acatcacgg 
      aacggtttg aagaggagg attatcaag acatgatgc 
      agcagcaga acaggcccg tgacccaaa acttgaacc 
      gcgaacgca agaaatacc agcacccca agggggcgc 
      ccgacgccg acctgattt ttggaccta agagagtta 
      gataatggg aggaattca ggatgaaat ggttgaata 
      cgattaagg gtcaaagaa gttcagaat gtttcagaa 
      atagataga ttgtttcgt ttttagtct atcggaatt 
      gagaattgt gaaggagaa gcaaggaat ttcattttt 
      tgtttcgtt caac