Status
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Gene information
General information
TC002832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8740129..8742357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002832
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002832 RA
      MKAIFQQLL TISFLFRCV LSGFECKES VDSTREVCK 
      TELRCRFDE DYDRIEKRF CRCKITIEH FPLPDNTLT 
      FDLFSGICF QRFNNLKQL WIQHADLQY LDNNIFEKY 
      PNLVHLDLS DNQVSLNFS KHAEKITTL NASYNKIDE 
      LKDEALADL KSLRVVDLS FNRLAQIGT GLKNLKSVE 
      EVYMNNNQL TQIEENDFE PLQKLRILN LAHNFQVSI 
      KSPLFFLHL QELYLNNNR LKSINAQEF VRLPSLRIL 
      KIGNNLFET ISDNHLFEQ LEEFHGENN QIKALNGHI 
      LNKLENLKM VNLSNNALK NIEGDTFAT SNRIKVVDL 
      SSNKLAKID PRNFRNMNN VSFLNLHNN SLTQEVISC 
      DNLQNLMQL NVLDLSYNE ISEIYTCTF RKLEKLQKL 
      SLLANHLKT IPPSAFQTL HNLQELNLA HNQLTEIPE 
      NSFQGLGEL RNLNISHNR LTKFDIDSL DGSPKVTKF 
      DLKCNNLTE IDLKLLRDK QTVLRELDF DDSLIKCDA 
      FGEVFYYFE KNEITVRSC AASGCCKNH ISQKLLIGI 
      IVGVIVCLL AAFGLIVIL YKLLCGRKK EPKIHLVPI 
      IQNQNH
    CDS sequence
    • >TC002832 RA
      atgaaagca atcttccaa caactcctg accattagt 
      ttcctcttc cgctgcgtt ttatctgga tttgagtgc 
      aaagaaagc gtggattca actcgagaa gtgtgcaaa 
      acggaatta aggtgccgt tttgatgaa gattacgac 
      cggattgaa aagagattt tgccggtgt aaaatcacg 
      attgagcat tttcccctt ccggacaac acgttaact 
      tttgactta ttttccgga atttgtttc caacgtttc 
      aataatctg aaacaactg tggatccaa cacgcagac 
      ctacaatac ttggacaac aacattttt gaaaagtac 
      ccaaattta gtgcatttg gatttgagt gacaaccaa 
      gtcagttta aatttctcc aaacacgcc gaaaaaata 
      accactctc aatgcaagt tacaacaaa atagatgaa 
      ttaaaagac gaagctttg gcagatttg aagagttta 
      agagtggtc gatttgtcg ttcaatcgt cttgcgcaa 
      atcggaaca ggtttaaaa aatttgaag agtgtggaa 
      gaggtttat atgaataat aatcaactg actcagatt 
      gaagaaaac gactttgaa cctttacaa aaactgagg 
      atcttaaat ctggctcac aatttccag gtttccatc 
      aaaagcccg ctgttcttt cttcacctc caagaactc 
      tacttgaac aataaccga ctgaaaagt ataaacgcc 
      caagagttc gtccgattg ccaagtctg aggattctc 
      aaaatcgga aataattta ttcgaaaca ataagtgac 
      aatcattta tttgaacaa ctggaagag tttcacgga 
      gaaaataac caaatcaag gcgcttaac ggacacatt 
      ttaaacaaa ctcgagaat ttaaaaatg gtcaatttg 
      tccaacaac gctttaaaa aacatagaa ggtgacacc 
      tttgctacc tcaaaccga atcaaagta gttgatttg 
      agttcgaac aaacttgct aaaattgac ccaagaaac 
      tttcggaat atgaacaat gtgtctttc ttgaatttg 
      cacaacaat tctctaaca caggaggtc atcagctgc 
      gacaatctg caaaattta atgcaattg aacgtactg 
      gacctctcc tacaacgaa atttcagaa atctacact 
      tgcactttt agaaaatta gaaaagttg caaaaattg 
      tcgttactg gccaaccat ttgaagacc atcccacca 
      tcagcattc caaactttg cacaatctc caagaacta 
      aatttagcc cacaatcaa ctaactgaa attccagaa 
      aattcattc caaggttta ggagagtta agaaattta 
      aatatttca cacaaccga ctgacaaaa ttcgatatt 
      gactctctt gacggaagc ccaaaagtg acaaaattt 
      gaccttaaa tgcaacaat ttaaccgaa atcgatttg 
      aaacttctt cgggataaa caaactgtg ttaagggaa 
      ctcgatttt gacgattcg ctaattaag tgtgatgcc 
      tttggtgaa gtgttttat tattttgaa aaaaacgag 
      atcactgtg cgaagttgt gcggcttca gggtgttgc 
      aaaaatcat atcagccaa aaattgttg atagggatt 
      attgtaggt gtgattgtt tgcctactt gcggctttt 
      ggcttaatt gtgatcctt tataagttg ctatgcggc 
      cgtaaaaaa gaaccgaaa attcattta gtgccgatt 
      attcaaaat caaaatcac tga
    mRNA sequence
    • >TC002832 RA
      aatagattt attattaca cttcttatc acttttatg 
      aagacagca acacaaaac tttaatctc ggaatttca 
      gacgattcg ccaacaatc tatttaaaa cggaattcc 
      cgtttattt gatttcgtt tcatttatt tattcgtac 
      cacaacaaa gtttaaatt agttgtcac aaaatcgta 
      gtgaagtaa cctagtcat gaaagcaat cttccaaca 
      actcctgac cattagttt cctcttccg ctgcgtttt 
      atctggatt tgagtgcaa agaaagcgt ggattcaac 
      tcgagaagt gtgcaaaac ggaattaag gtgccgttt 
      tgatgaaga ttacgaccg gattgaaaa gagattttg 
      ccggtgtaa aatcacgat tgagcattt tccccttcc 
      ggacaacac gttaacttt tgacttatt ttccggaat 
      ttgtttcca acgtttcaa taatctgaa acaactgtg 
      gatccaaca cgcagacct acaatactt ggacaacaa 
      catttttga aaagtaccc aaatttagt gcatttgga 
      tttgagtga caaccaagt cagtttaaa tttctccaa 
      acacgccga aaaaataac cactctcaa tgcaagtta 
      caacaaaat agatgaatt aaaagacga agctttggc 
      agatttgaa gagtttaag agtggtcga tttgtcgtt 
      caatcgtct tgcgcaaat cggaacagg tttaaaaaa 
      tttgaagag tgtggaaga ggtttatat gaataataa 
      tcaactgac tcagattga agaaaacga ctttgaacc 
      tttacaaaa actgaggat cttaaatct ggctcacaa 
      tttccaggt ttccatcaa aagcccgct gttctttct 
      tcacctcca agaactcta cttgaacaa taaccgact 
      gaaaagtat aaacgccca agagttcgt ccgattgcc 
      aagtctgag gattctcaa aatcggaaa taatttatt 
      cgaaacaat aagtgacaa tcatttatt tgaacaact 
      ggaagagtt tcacggaga aaataacca aatcaaggc 
      gcttaacgg acacatttt aaacaaact cgagaattt 
      aaaaatggt caatttgtc caacaacgc tttaaaaaa 
      catagaagg tgacacctt tgctacctc aaaccgaat 
      caaagtagt tgatttgag ttcgaacaa acttgctaa 
      aattgaccc aagaaactt tcggaatat gaacaatgt 
      gtctttctt gaatttgca caacaattc tctaacaca 
      ggaggtcat cagctgcga caatctgca aaatttaat 
      gcaattgaa cgtactgga cctctccta caacgaaat 
      ttcagaaat ctacacttg cacttttag aaaattaga 
      aaagttgca aaaattgtc gttactggc caaccattt 
      gaagaccat cccaccatc agcattcca aactttgca 
      caatctcca agaactaaa tttagccca caatcaact 
      aactgaaat tccagaaaa ttcattcca aggtttagg 
      agagttaag aaatttaaa tatttcaca caaccgact 
      gacaaaatt cgatattga ctctcttga cggaagccc 
      aaaagtgac aaaatttga ccttaaatg caacaattt 
      aaccgaaat cgatttgaa acttcttcg ggataaaca 
      aactgtgtt aagggaact cgattttga cgattcgct 
      aattaagtg tgatgcctt tggtgaagt gttttatta 
      ttttgaaaa aaacgagat cactgtgcg aagttgtgc 
      ggcttcagg gtgttgcaa aaatcatat cagccaaaa 
      attgttgat agggattat tgtaggtgt gattgtttg 
      cctacttgc ggcttttgg cttaattgt gatccttta 
      taagttgct atgcggccg taaaaaaga accgaaaat 
      tcatttagt gccgattat tcaaaatca aaatcactg 
      agttgtttt tatcgaata gactgtatt gttacgtta 
      atgatgatt aattaatta atgattatt gaatgatac 
      gcaaaaaaa gtagttaca agtacctac cacaaacta 
      ttatgcctt gttttggat ttaaaaaat aaacaaata 
      aactgtttt tagaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY