General information | |||
TC002832 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 8740129..8742357 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002832 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00451 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC002832 RA
MKAIFQQLL TISFLFRCV LSGFECKES VDSTREVCK TELRCRFDE DYDRIEKRF CRCKITIEH FPLPDNTLT FDLFSGICF QRFNNLKQL WIQHADLQY LDNNIFEKY PNLVHLDLS DNQVSLNFS KHAEKITTL NASYNKIDE LKDEALADL KSLRVVDLS FNRLAQIGT GLKNLKSVE EVYMNNNQL TQIEENDFE PLQKLRILN LAHNFQVSI KSPLFFLHL QELYLNNNR LKSINAQEF VRLPSLRIL KIGNNLFET ISDNHLFEQ LEEFHGENN QIKALNGHI LNKLENLKM VNLSNNALK NIEGDTFAT SNRIKVVDL SSNKLAKID PRNFRNMNN VSFLNLHNN SLTQEVISC DNLQNLMQL NVLDLSYNE ISEIYTCTF RKLEKLQKL SLLANHLKT IPPSAFQTL HNLQELNLA HNQLTEIPE NSFQGLGEL RNLNISHNR LTKFDIDSL DGSPKVTKF DLKCNNLTE IDLKLLRDK QTVLRELDF DDSLIKCDA FGEVFYYFE KNEITVRSC AASGCCKNH ISQKLLIGI IVGVIVCLL AAFGLIVIL YKLLCGRKK EPKIHLVPI IQNQNH
- >TC002832 RA
atgaaagca atcttccaa caactcctg accattagt ttcctcttc cgctgcgtt ttatctgga tttgagtgc aaagaaagc gtggattca actcgagaa gtgtgcaaa acggaatta aggtgccgt tttgatgaa gattacgac cggattgaa aagagattt tgccggtgt aaaatcacg attgagcat tttcccctt ccggacaac acgttaact tttgactta ttttccgga atttgtttc caacgtttc aataatctg aaacaactg tggatccaa cacgcagac ctacaatac ttggacaac aacattttt gaaaagtac ccaaattta gtgcatttg gatttgagt gacaaccaa gtcagttta aatttctcc aaacacgcc gaaaaaata accactctc aatgcaagt tacaacaaa atagatgaa ttaaaagac gaagctttg gcagatttg aagagttta agagtggtc gatttgtcg ttcaatcgt cttgcgcaa atcggaaca ggtttaaaa aatttgaag agtgtggaa gaggtttat atgaataat aatcaactg actcagatt gaagaaaac gactttgaa cctttacaa aaactgagg atcttaaat ctggctcac aatttccag gtttccatc aaaagcccg ctgttcttt cttcacctc caagaactc tacttgaac aataaccga ctgaaaagt ataaacgcc caagagttc gtccgattg ccaagtctg aggattctc aaaatcgga aataattta ttcgaaaca ataagtgac aatcattta tttgaacaa ctggaagag tttcacgga gaaaataac caaatcaag gcgcttaac ggacacatt ttaaacaaa ctcgagaat ttaaaaatg gtcaatttg tccaacaac gctttaaaa aacatagaa ggtgacacc tttgctacc tcaaaccga atcaaagta gttgatttg agttcgaac aaacttgct aaaattgac ccaagaaac tttcggaat atgaacaat gtgtctttc ttgaatttg cacaacaat tctctaaca caggaggtc atcagctgc gacaatctg caaaattta atgcaattg aacgtactg gacctctcc tacaacgaa atttcagaa atctacact tgcactttt agaaaatta gaaaagttg caaaaattg tcgttactg gccaaccat ttgaagacc atcccacca tcagcattc caaactttg cacaatctc caagaacta aatttagcc cacaatcaa ctaactgaa attccagaa aattcattc caaggttta ggagagtta agaaattta aatatttca cacaaccga ctgacaaaa ttcgatatt gactctctt gacggaagc ccaaaagtg acaaaattt gaccttaaa tgcaacaat ttaaccgaa atcgatttg aaacttctt cgggataaa caaactgtg ttaagggaa ctcgatttt gacgattcg ctaattaag tgtgatgcc tttggtgaa gtgttttat tattttgaa aaaaacgag atcactgtg cgaagttgt gcggcttca gggtgttgc aaaaatcat atcagccaa aaattgttg atagggatt attgtaggt gtgattgtt tgcctactt gcggctttt ggcttaatt gtgatcctt tataagttg ctatgcggc cgtaaaaaa gaaccgaaa attcattta gtgccgatt attcaaaat caaaatcac tga
- >TC002832 RA
aatagattt attattaca cttcttatc acttttatg aagacagca acacaaaac tttaatctc ggaatttca gacgattcg ccaacaatc tatttaaaa cggaattcc cgtttattt gatttcgtt tcatttatt tattcgtac cacaacaaa gtttaaatt agttgtcac aaaatcgta gtgaagtaa cctagtcat gaaagcaat cttccaaca actcctgac cattagttt cctcttccg ctgcgtttt atctggatt tgagtgcaa agaaagcgt ggattcaac tcgagaagt gtgcaaaac ggaattaag gtgccgttt tgatgaaga ttacgaccg gattgaaaa gagattttg ccggtgtaa aatcacgat tgagcattt tccccttcc ggacaacac gttaacttt tgacttatt ttccggaat ttgtttcca acgtttcaa taatctgaa acaactgtg gatccaaca cgcagacct acaatactt ggacaacaa catttttga aaagtaccc aaatttagt gcatttgga tttgagtga caaccaagt cagtttaaa tttctccaa acacgccga aaaaataac cactctcaa tgcaagtta caacaaaat agatgaatt aaaagacga agctttggc agatttgaa gagtttaag agtggtcga tttgtcgtt caatcgtct tgcgcaaat cggaacagg tttaaaaaa tttgaagag tgtggaaga ggtttatat gaataataa tcaactgac tcagattga agaaaacga ctttgaacc tttacaaaa actgaggat cttaaatct ggctcacaa tttccaggt ttccatcaa aagcccgct gttctttct tcacctcca agaactcta cttgaacaa taaccgact gaaaagtat aaacgccca agagttcgt ccgattgcc aagtctgag gattctcaa aatcggaaa taatttatt cgaaacaat aagtgacaa tcatttatt tgaacaact ggaagagtt tcacggaga aaataacca aatcaaggc gcttaacgg acacatttt aaacaaact cgagaattt aaaaatggt caatttgtc caacaacgc tttaaaaaa catagaagg tgacacctt tgctacctc aaaccgaat caaagtagt tgatttgag ttcgaacaa acttgctaa aattgaccc aagaaactt tcggaatat gaacaatgt gtctttctt gaatttgca caacaattc tctaacaca ggaggtcat cagctgcga caatctgca aaatttaat gcaattgaa cgtactgga cctctccta caacgaaat ttcagaaat ctacacttg cacttttag aaaattaga aaagttgca aaaattgtc gttactggc caaccattt gaagaccat cccaccatc agcattcca aactttgca caatctcca agaactaaa tttagccca caatcaact aactgaaat tccagaaaa ttcattcca aggtttagg agagttaag aaatttaaa tatttcaca caaccgact gacaaaatt cgatattga ctctcttga cggaagccc aaaagtgac aaaatttga ccttaaatg caacaattt aaccgaaat cgatttgaa acttcttcg ggataaaca aactgtgtt aagggaact cgattttga cgattcgct aattaagtg tgatgcctt tggtgaagt gttttatta ttttgaaaa aaacgagat cactgtgcg aagttgtgc ggcttcagg gtgttgcaa aaatcatat cagccaaaa attgttgat agggattat tgtaggtgt gattgtttg cctacttgc ggcttttgg cttaattgt gatccttta taagttgct atgcggccg taaaaaaga accgaaaat tcatttagt gccgattat tcaaaatca aaatcactg agttgtttt tatcgaata gactgtatt gttacgtta atgatgatt aattaatta atgattatt gaatgatac gcaaaaaaa gtagttaca agtacctac cacaaacta ttatgcctt gttttggat ttaaaaaat aaacaaata aactgtttt tagaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
