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Gene information
General information
TC002832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8740129..8742357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002832
    iBeetle screen iB_00451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002832 RA
      MKAIFQQLL TISFLFRCV LSGFECKES VDSTREVCK 
TELRCRFDE DYDRIEKRF CRCKITIEH FPLPDNTLT 
FDLFSGICF QRFNNLKQL WIQHADLQY LDNNIFEKY 
PNLVHLDLS DNQVSLNFS KHAEKITTL NASYNKIDE 
LKDEALADL KSLRVVDLS FNRLAQIGT GLKNLKSVE 
EVYMNNNQL TQIEENDFE PLQKLRILN LAHNFQVSI 
KSPLFFLHL QELYLNNNR LKSINAQEF VRLPSLRIL 
KIGNNLFET ISDNHLFEQ LEEFHGENN QIKALNGHI 
LNKLENLKM VNLSNNALK NIEGDTFAT SNRIKVVDL 
SSNKLAKID PRNFRNMNN VSFLNLHNN SLTQEVISC 
DNLQNLMQL NVLDLSYNE ISEIYTCTF RKLEKLQKL 
SLLANHLKT IPPSAFQTL HNLQELNLA HNQLTEIPE 
NSFQGLGEL RNLNISHNR LTKFDIDSL DGSPKVTKF 
DLKCNNLTE IDLKLLRDK QTVLRELDF DDSLIKCDA 
FGEVFYYFE KNEITVRSC AASGCCKNH ISQKLLIGI 
IVGVIVCLL AAFGLIVIL YKLLCGRKK EPKIHLVPI 
IQNQNH
    CDS sequence
    • >TC002832 RA
      atgaaagca atcttccaa caactcctg accattagt 
ttcctcttc cgctgcgtt ttatctgga tttgagtgc 
aaagaaagc gtggattca actcgagaa gtgtgcaaa 
acggaatta aggtgccgt tttgatgaa gattacgac 
cggattgaa aagagattt tgccggtgt aaaatcacg 
attgagcat tttcccctt ccggacaac acgttaact 
tttgactta ttttccgga atttgtttc caacgtttc 
aataatctg aaacaactg tggatccaa cacgcagac 
ctacaatac ttggacaac aacattttt gaaaagtac 
ccaaattta gtgcatttg gatttgagt gacaaccaa 
gtcagttta aatttctcc aaacacgcc gaaaaaata 
accactctc aatgcaagt tacaacaaa atagatgaa 
ttaaaagac gaagctttg gcagatttg aagagttta 
agagtggtc gatttgtcg ttcaatcgt cttgcgcaa 
atcggaaca ggtttaaaa aatttgaag agtgtggaa 
gaggtttat atgaataat aatcaactg actcagatt 
gaagaaaac gactttgaa cctttacaa aaactgagg 
atcttaaat ctggctcac aatttccag gtttccatc 
aaaagcccg ctgttcttt cttcacctc caagaactc 
tacttgaac aataaccga ctgaaaagt ataaacgcc 
caagagttc gtccgattg ccaagtctg aggattctc 
aaaatcgga aataattta ttcgaaaca ataagtgac 
aatcattta tttgaacaa ctggaagag tttcacgga 
gaaaataac caaatcaag gcgcttaac ggacacatt 
ttaaacaaa ctcgagaat ttaaaaatg gtcaatttg 
tccaacaac gctttaaaa aacatagaa ggtgacacc 
tttgctacc tcaaaccga atcaaagta gttgatttg 
agttcgaac aaacttgct aaaattgac ccaagaaac 
tttcggaat atgaacaat gtgtctttc ttgaatttg 
cacaacaat tctctaaca caggaggtc atcagctgc 
gacaatctg caaaattta atgcaattg aacgtactg 
gacctctcc tacaacgaa atttcagaa atctacact 
tgcactttt agaaaatta gaaaagttg caaaaattg 
tcgttactg gccaaccat ttgaagacc atcccacca 
tcagcattc caaactttg cacaatctc caagaacta 
aatttagcc cacaatcaa ctaactgaa attccagaa 
aattcattc caaggttta ggagagtta agaaattta 
aatatttca cacaaccga ctgacaaaa ttcgatatt 
gactctctt gacggaagc ccaaaagtg acaaaattt 
gaccttaaa tgcaacaat ttaaccgaa atcgatttg 
aaacttctt cgggataaa caaactgtg ttaagggaa 
ctcgatttt gacgattcg ctaattaag tgtgatgcc 
tttggtgaa gtgttttat tattttgaa aaaaacgag 
atcactgtg cgaagttgt gcggcttca gggtgttgc 
aaaaatcat atcagccaa aaattgttg atagggatt 
attgtaggt gtgattgtt tgcctactt gcggctttt 
ggcttaatt gtgatcctt tataagttg ctatgcggc 
cgtaaaaaa gaaccgaaa attcattta gtgccgatt 
attcaaaat caaaatcac tga
    mRNA sequence
    • >TC002832 RA
      aatagattt attattaca cttcttatc acttttatg 
aagacagca acacaaaac tttaatctc ggaatttca 
gacgattcg ccaacaatc tatttaaaa cggaattcc 
cgtttattt gatttcgtt tcatttatt tattcgtac 
cacaacaaa gtttaaatt agttgtcac aaaatcgta 
gtgaagtaa cctagtcat gaaagcaat cttccaaca 
actcctgac cattagttt cctcttccg ctgcgtttt 
atctggatt tgagtgcaa agaaagcgt ggattcaac 
tcgagaagt gtgcaaaac ggaattaag gtgccgttt 
tgatgaaga ttacgaccg gattgaaaa gagattttg 
ccggtgtaa aatcacgat tgagcattt tccccttcc 
ggacaacac gttaacttt tgacttatt ttccggaat 
ttgtttcca acgtttcaa taatctgaa acaactgtg 
gatccaaca cgcagacct acaatactt ggacaacaa 
catttttga aaagtaccc aaatttagt gcatttgga 
tttgagtga caaccaagt cagtttaaa tttctccaa 
acacgccga aaaaataac cactctcaa tgcaagtta 
caacaaaat agatgaatt aaaagacga agctttggc 
agatttgaa gagtttaag agtggtcga tttgtcgtt 
caatcgtct tgcgcaaat cggaacagg tttaaaaaa 
tttgaagag tgtggaaga ggtttatat gaataataa 
tcaactgac tcagattga agaaaacga ctttgaacc 
tttacaaaa actgaggat cttaaatct ggctcacaa 
tttccaggt ttccatcaa aagcccgct gttctttct 
tcacctcca agaactcta cttgaacaa taaccgact 
gaaaagtat aaacgccca agagttcgt ccgattgcc 
aagtctgag gattctcaa aatcggaaa taatttatt 
cgaaacaat aagtgacaa tcatttatt tgaacaact 
ggaagagtt tcacggaga aaataacca aatcaaggc 
gcttaacgg acacatttt aaacaaact cgagaattt 
aaaaatggt caatttgtc caacaacgc tttaaaaaa 
catagaagg tgacacctt tgctacctc aaaccgaat 
caaagtagt tgatttgag ttcgaacaa acttgctaa 
aattgaccc aagaaactt tcggaatat gaacaatgt 
gtctttctt gaatttgca caacaattc tctaacaca 
ggaggtcat cagctgcga caatctgca aaatttaat 
gcaattgaa cgtactgga cctctccta caacgaaat 
ttcagaaat ctacacttg cacttttag aaaattaga 
aaagttgca aaaattgtc gttactggc caaccattt 
gaagaccat cccaccatc agcattcca aactttgca 
caatctcca agaactaaa tttagccca caatcaact 
aactgaaat tccagaaaa ttcattcca aggtttagg 
agagttaag aaatttaaa tatttcaca caaccgact 
gacaaaatt cgatattga ctctcttga cggaagccc 
aaaagtgac aaaatttga ccttaaatg caacaattt 
aaccgaaat cgatttgaa acttcttcg ggataaaca 
aactgtgtt aagggaact cgattttga cgattcgct 
aattaagtg tgatgcctt tggtgaagt gttttatta 
ttttgaaaa aaacgagat cactgtgcg aagttgtgc 
ggcttcagg gtgttgcaa aaatcatat cagccaaaa 
attgttgat agggattat tgtaggtgt gattgtttg 
cctacttgc ggcttttgg cttaattgt gatccttta 
taagttgct atgcggccg taaaaaaga accgaaaat 
tcatttagt gccgattat tcaaaatca aaatcactg 
agttgtttt tatcgaata gactgtatt gttacgtta 
atgatgatt aattaatta atgattatt gaatgatac 
gcaaaaaaa gtagttaca agtacctac cacaaacta 
ttatgcctt gttttggat ttaaaaaat aaacaaata 
aactgtttt tagaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY