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Gene information
General information
TC002828 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8802241..8825527
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002828
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00450

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002828 RA
      MAPHSRSLW LVLVLRAGL ASAQEAACY GSGSIALSV 
ILSVVFTAV LLAALLYLW GKYRKRKEL ILETDPEKG 
KGGEYAFDN PAFKDPGLT PIGKLEKEK VDANKTKWT 
QWSPLTALT TKSEKKKTL DDSAIDGNL VKVVALRSH 
DFTGLGFNI CGNMKEGIY IKDVLHRGP ASESGKLNP 
GDRINSVTI SFEHMVYED ALTILSYAS PYEVIIEAR 
GGKMFQTSG QGAQPSHPV YRSSSCTEL FQVNSAKRK 
LFGTEEHSG SLSSNYSSL QKSRSNMTT LERKESESP 
RNRPKKNPP KTQMSPEQL KTQLEQRIM SDHQHNLKN 
KVEPKVEME VQKTENKLQ KFGIRVLPP EKSPKSAEL 
SQNENNLNL EKLPIGLDE VDDKKPQVK KRERKLTPE 
IFDRDHVNN SSGIKRDAN GIPQEIPNY MMNAAVAAR 
SNRKSSVKD EEDKTPKKT KGKAPSPPE FKNLDEIQP 
VDDFEEIAP KPTQDDLVE EIEVELRNK TMKDYNSDS 
DLETDNHSS VNTIELNSS DITIHHAED TEDRQNRRT 
ASTGDLTKI QRTRKSSTG TLERAQSLD ITDTSIPTL 
TKKRKGRIE DDLMASEED IFGKVVMSK EPRLSLMLD 
GLNTFQRNR LKKSTEWGN LEDAILKLN QEDESDPKT 
DLDLSGKTE EIDAVVNKI NEIKRESLE VQPPIKEET 
PPPKPEKKV KNQIWPFET KANGSAIVD DNPRLASVE 
KRQRKPPIL DPVKPAVPE KKDHLLAKK ILQNFIFTE 
RIACSQDDD DTNVSDDIK VSRHSLGSL ERAKSDDAK 
INVSNVTVN NLRPQSEEN LHSLEIDTT EPELYTTAL 
DSTVKPEPE TPKVTISTP DIIKNVTIA EAINKLNND 
VMVQGPSSL TLEISNDAE IKDNRFMVD VKPDRVKKE 
DSGSETKSF EDDSSKSNS SLTYITEIQ VLTPNNSTS 
TNISEIEII PNVNSNSNG KTLDLEHEF EDYVRNFES 
NVKTFESNI QKFENHLDS TPKPDLKSI IITENDAEK 
ELHKIQEIA EEQLKKLPE MRFTTSSYE PSKLPEKRQ 
SQIELLRSN FEKSPPKSK PDTPKSRIP IATTAKTPP 
TSPERRDSR NLDMENDKD ILELMSSTV HSTPKIKPP 
KNVTVTSIR SNSKIPSGL PVLGGRPAV PPRRQEEDH 
VVQVSTNGG VESSFKQWV FNPTDAVTN IVVAESKQQ 
K
    CDS sequence
    • >TC002828 RA
      atggcaccg cattctcgc agtttgtgg ttggtgctg 
gtgctcagg gccgggctg gccagcgct caggaggcc 
gcctgttac ggatctgga tccatcgcg ctctcggtc 
atcctgtcc gtcgtcttc acggccgtt ctgttggcg 
gcgctgctc tacctctgg gggaagtac cggaaacgg 
aaggagctc atcctcgaa accgacccc gagaaaggc 
aaaggcggc gaatacgca ttcgacaat ccagcgttc 
aaagatcct ggactaact ccgatcggg aaattggag 
aaggaaaag gtcgacgcg aacaagacg aaatggacg 
cagtggtcg cccctgacg gccctcacc accaagtcc 
gagaaaaag aagactttg gatgacagt gccattgat 
ggaaatttg gtgaaagta gtggcgtta cgcagccat 
gatttcacc ggactgggg ttcaacatt tgtggcaac 
atgaaagaa gggatttac atcaaggat gtcctgcac 
cgagggcct gcctccgag tcaggcaaa ctcaatccc 
ggtgataga atcaacagt gtcaccatt agtttcgaa 
cacatggta tacgaagat gctttaaca attttaagt 
tatgctagt ccctatgaa gtcataatc gaagcacga 
ggcgggaaa atgttccaa acaagtggg caaggggcc 
caaccaagt caccccgtg tacaggagc tcctcttgt 
accgaactc ttccaagtt aactccgcc aaacgtaaa 
ttatttggc accgaagaa cactccggt tccctcagt 
tcgaattac tcaagtctc cagaaatct agaagtaac 
atgacgacc cttgaacgc aaggaatcc gaatctccc 
cgaaaccgc cccaagaaa aaccccccg aagacacaa 
atgtccccc gaacagtta aaaacgcag cttgagcaa 
agaataatg tcagaccac caacacaac ttgaaaaac 
aaagtcgag ccaaaagtc gaaatggag gtccaaaaa 
accgagaac aagttgcaa aaattcggc attagggtc 
ctcccgcct gagaagagc cccaaatcg gccgagctg 
tcccaaaac gagaacaac ctcaacttg gagaaactc 
cccatcgga cttgacgaa gtcgacgat aagaaacca 
caagtcaaa aagcgggaa cgcaaacta acccccgaa 
attttcgat cgggaccac gtaaacaac agttccggg 
atcaaacgg gacgccaac ggaatcccc caggagatc 
cccaactac atgatgaac gcggccgtt gccgcccgc 
agtaaccgc aaaagctcg gtcaaagac gaggaggac 
aaaaccccc aagaaaacc aaaggcaag gcgccgtca 
cctcccgaa ttcaaaaac ctggatgag atacaacca 
gttgacgat ttcgaggaa atcgctccc aagcccacc 
caagacgac ttagttgag gagattgag gtcgagttg 
aggaacaaa acaatgaaa gactacaac tcggactcg 
gatctcgaa accgacaat cactcctct gtcaacacc 
atcgagctg aattcgagc gacatcacc atccaccac 
gccgaagac accgaagac cgccaaaac cgccgaact 
gcaagcacc ggcgacttg accaagatc cagcgcacg 
cggaaatcc agcactggc accctggag cgcgcccag 
agcctggac atcaccgac accagcata ccaaccctg 
accaagaag cgcaaaggg cggatcgag gacgacttg 
atggcttcc gaggaggat attttcgga aaagtggtc 
atgagtaaa gaaccccgc ctgtcgctg atgctcgac 
ggcttgaac acgttccag cgaaaccgc ctgaagaag 
tcaaccgag tgggggaac ctcgaggac gccatcctc 
aagctgaac caagaggac gaaagcgac ccgaagacc 
gacctggac ctcagcggc aagaccgag gaaatcgac 
gctgttgtg aacaaaatc aacgaaatc aagcgcgag 
tccctggag gtgcagccg cccatcaag gaggaaacc 
ccgccgcct aagcccgag aagaaggtc aagaaccag 
atctggccg ttcgagacc aaggcgaac ggaagtgcc 
atagtggac gataacccg cggcttgcc tcggtggag 
aagcggcag cgcaagccc ccgattttg gacccagtg 
aagccggcg gttcccgaa aagaaggac cacctcctg 
gcgaaaaaa atcctccag aattttatc ttcacggag 
aggatcgcc tgcagtcag gacgacgac gatacgaac 
gtctccgac gatattaag gtctccagg cactccttg 
gggagcctg gagcgggca aagtccgac gacgccaag 
ataaacgtt agcaatgtc actgttaat aatctccga 
ccgcaaagc gaggagaat ctgcacagt ttggaaatt 
gacacgact gagcccgag ttgtacacc acggcgctt 
gatagtacg gttaaaccg gagcccgaa acgcccaaa 
gtcacgata agtaccccc gacatcatc aaaaatgtc 
acaatcgcc gaagcgata aataagtta aacaacgat 
gtgatggtt cagggaccg tcgtcactg actttggag 
atttcgaac gatgcggaa atcaaggac aatcgattt 
atggtcgat gttaaaccg gatcgagtg aagaaggaa 
gattcggga agcgagacg aagagtttt gaggacgat 
agtagcaaa agtaactcg tccctgact tacatcacc 
gagatccag gtcttgact cctaacaac tcgaccagt 
accaacatt tctgagatc gaaatcatc cccaatgtt 
aacagcaat agcaatggc aaaaccctg gacctggag 
cacgaattc gaggactac gtccgcaac ttcgaatcc 
aacgtgaaa accttcgag tccaacatc caaaagttt 
gaaaaccac ctggactcg accccgaaa cccgacttg 
aagagcatc ataataacg gaaaacgac gccgaaaag 
gaactccac aaaatccaa gaaatcgca gaggagcaa 
ctgaagaaa ctccccgaa atgcgcttc acaacatcc 
agctacgag ccatcgaaa ctcccggag aagcgccag 
tcgcagatc gagctcctg cgttcaaac ttcgagaaa 
agcccgccc aagtccaag ccagacacg cccaagtcg 
cgcattccc atcgcgacc acggccaaa actccgccc 
acctcgccg gaaaggcgc gattcgcgc aatctggac 
atggaaaac gacaaggac atcctcgag ttgatgtcg 
tcgacggtc cactccacc cccaagata aaaccgccc 
aaaaacgtc acagtcact tcgattcgg agcaattcg 
aagattccg tccggactg cccgttttg ggcgggagg 
ccggcggtg ccgccccgt aggcaggag gaagaccac 
gtggtgcag gtctcgacc aatggcgga gtggagagt 
agttttaag cagtgggtg tttaatccc accgatgcc 
gtgacgaat atcgtcgtg gctgagagt aaacagcag 
aagtga
    mRNA sequence
    • >TC002828 RA
      agtgcccct atcccgccc cctttatct gcaactggg 
ggcaatccc ttgcaataa ataactacc cccttccac 
tggccccaa gcacccctc ggccacatc cggaaaacc 
cccaattgc ggtcggaat gcgcccccc tcggcccat 
cttcctcgc cgcaataat cagacaggt ttcggtccc 
agtcggtca ttcaaggta gtaagttca aagatagcc 
tcaggtgca cacacacac atacacaca cctgccagg 
cctccgagt cggtgcctc acagatagt cgcatgttc 
gtccacctg tcgggtgta aacaaagta gtccgtggt 
gcagtgtga tcgtcacat gtttttccc ggttttcgc 
ttgtttcgg gatgggtga tggtgctgt gctgggatt 
aagggacac ggattaggg acactggga ccaaaccgc 
aatgaagaa cggccgttg agaacctcc gtgaaaaac 
gttcgaaat ggcaccgca ttctcgcag tttgtggtt 
ggtgctggt gctcagggc cgggctggc cagcgctca 
ggaggccgc ctgttacgg atctggatc catcgcgct 
ctcggtcat cctgtccgt cgtcttcac ggccgttct 
gttggcggc gctgctcta cctctgggg gaagtaccg 
gaaacggaa ggagctcat cctcgaaac cgaccccga 
gaaaggcaa aggcggcga atacgcatt cgacaatcc 
agcgttcaa agatcctgg actaactcc gatcgggaa 
attggagaa ggaaaaggt cgacgcgaa caagacgaa 
atggacgca gtggtcgcc cctgacggc cctcaccac 
caagtccga gaaaaagaa gactttgga tgacagtgc 
cattgatgg aaatttggt gaaagtagt ggcgttacg 
cagccatga tttcaccgg actggggtt caacatttg 
tggcaacat gaaagaagg gatttacat caaggatgt 
cctgcaccg agggcctgc ctccgagtc aggcaaact 
caatcccgg tgatagaat caacagtgt caccattag 
tttcgaaca catggtata cgaagatgc tttaacaat 
tttaagtta tgctagtcc ctatgaagt cataatcga 
agcacgagg cgggaaaat gttccaaac aagtgggca 
aggggccca accaagtca ccccgtgta caggagctc 
ctcttgtac cgaactctt ccaagttaa ctccgccaa 
acgtaaatt atttggcac cgaagaaca ctccggttc 
cctcagttc gaattactc aagtctcca gaaatctag 
aagtaacat gacgaccct tgaacgcaa ggaatccga 
atctccccg aaaccgccc caagaaaaa ccccccgaa 
gacacaaat gtcccccga acagttaaa aacgcagct 
tgagcaaag aataatgtc agaccacca acacaactt 
gaaaaacaa agtcgagcc aaaagtcga aatggaggt 
ccaaaaaac cgagaacaa gttgcaaaa attcggcat 
tagggtcct cccgcctga gaagagccc caaatcggc 
cgagctgtc ccaaaacga gaacaacct caacttgga 
gaaactccc catcggact tgacgaagt cgacgataa 
gaaaccaca agtcaaaaa gcgggaacg caaactaac 
ccccgaaat tttcgatcg ggaccacgt aaacaacag 
ttccgggat caaacggga cgccaacgg aatccccca 
ggagatccc caactacat gatgaacgc ggccgttgc 
cgcccgcag taaccgcaa aagctcggt caaagacga 
ggaggacaa aacccccaa gaaaaccaa aggcaaggc 
gccgtcacc tcccgaatt caaaaacct ggatgagat 
acaaccagt tgacgattt cgaggaaat cgctcccaa 
gcccaccca agacgactt agttgagga gattgaggt 
cgagttgag gaacaaaac aatgaaaga ctacaactc 
ggactcgga tctcgaaac cgacaatca ctcctctgt 
caacaccat cgagctgaa ttcgagcga catcaccat 
ccaccacgc cgaagacac cgaagaccg ccaaaaccg 
ccgaactgc aagcaccgg cgacttgac caagatcca 
gcgcacgcg gaaatccag cactggcac cctggagcg 
cgcccagag cctggacat caccgacac cagcatacc 
aaccctgac caagaagcg caaagggcg gatcgagga 
cgacttgat ggcttccga ggaggatat tttcggaaa 
agtggtcat gagtaaaga accccgcct gtcgctgat 
gctcgacgg cttgaacac gttccagcg aaaccgcct 
gaagaagtc aaccgagtg ggggaacct cgaggacgc 
catcctcaa gctgaacca agaggacga aagcgaccc 
gaagaccga cctggacct cagcggcaa gaccgagga 
aatcgacgc tgttgtgaa caaaatcaa cgaaatcaa 
gcgcgagtc cctggaggt gcagccgcc catcaagga 
ggaaacccc gccgcctaa gcccgagaa gaaggtcaa 
gaaccagat ctggccgtt cgagaccaa ggcgaacgg 
aagtgccat agtggacga taacccgcg gcttgcctc 
ggtggagaa gcggcagcg caagccccc gattttgga 
cccagtgaa gccggcggt tcccgaaaa gaaggacca 
cctcctggc gaaaaaaat cctccagaa ttttatctt 
cacggagag gatcgcctg cagtcagga cgacgacga 
tacgaacgt ctccgacga tattaaggt ctccaggca 
ctccttggg gagcctgga gcgggcaaa gtccgacga 
cgccaagat aaacgttag caatgtcac tgttaataa 
tctccgacc gcaaagcga ggagaatct gcacagttt 
ggaaattga cacgactga gcccgagtt gtacaccac 
ggcgcttga tagtacggt taaaccgga gcccgaaac 
gcccaaagt cacgataag tacccccga catcatcaa 
aaatgtcac aatcgccga agcgataaa taagttaaa 
caacgatgt gatggttca gggaccgtc gtcactgac 
tttggagat ttcgaacga tgcggaaat caaggacaa 
tcgatttat ggtcgatgt taaaccgga tcgagtgaa 
gaaggaaga ttcgggaag cgagacgaa gagttttga 
ggacgatag tagcaaaag taactcgtc cctgactta 
catcaccga gatccaggt cttgactcc taacaactc 
gaccagtac caacatttc tgagatcga aatcatccc 
caatgttaa cagcaatag caatggcaa aaccctgga 
cctggagca cgaattcga ggactacgt ccgcaactt 
cgaatccaa cgtgaaaac cttcgagtc caacatcca 
aaagtttga aaaccacct ggactcgac cccgaaacc 
cgacttgaa gagcatcat aataacgga aaacgacgc 
cgaaaagga actccacaa aatccaaga aatcgcaga 
ggagcaact gaagaaact ccccgaaat gcgcttcac 
aacatccag ctacgagcc atcgaaact cccggagaa 
gcgccagtc gcagatcga gctcctgcg ttcaaactt 
cgagaaaag cccgcccaa gtccaagcc agacacgcc 
caagtcgcg cattcccat cgcgaccac ggccaaaac 
tccgcccac ctcgccgga aaggcgcga ttcgcgcaa 
tctggacat ggaaaacga caaggacat cctcgagtt 
gatgtcgtc gacggtcca ctccacccc caagataaa 
accgcccaa aaacgtcac agtcacttc gattcggag 
caattcgaa gattccgtc cggactgcc cgttttggg 
cgggaggcc ggcggtgcc gccccgtag gcaggagga 
agaccacgt ggtgcaggt ctcgaccaa tggcggagt 
ggagagtag ttttaagca gtgggtgtt taatcccac 
cgatgccgt gacgaatat cgtcgtggc tgagagtaa 
acagcagaa gtgatgata cgtgcaatg gaagaggcg 
gctcaattt agtgtagtg gaattattt atataaaaa 
cgtagttgt atatatttg taaaagtaa tgtttatgt 
atgtgctat tattttaag ctgatgatt ttgttggtt 
tattttgta attttaatc gagggtgac tatacatta 
ataacaaaa agtacatgt ttttctaac cagagatag 
atatatcca ataaatgaa attaaaccc a
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