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Gene information
General information
TC002828 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8802241..8825527
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002828
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00450

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002828 RA
      MAPHSRSLW LVLVLRAGL ASAQEAACY GSGSIALSV 
      ILSVVFTAV LLAALLYLW GKYRKRKEL ILETDPEKG 
      KGGEYAFDN PAFKDPGLT PIGKLEKEK VDANKTKWT 
      QWSPLTALT TKSEKKKTL DDSAIDGNL VKVVALRSH 
      DFTGLGFNI CGNMKEGIY IKDVLHRGP ASESGKLNP 
      GDRINSVTI SFEHMVYED ALTILSYAS PYEVIIEAR 
      GGKMFQTSG QGAQPSHPV YRSSSCTEL FQVNSAKRK 
      LFGTEEHSG SLSSNYSSL QKSRSNMTT LERKESESP 
      RNRPKKNPP KTQMSPEQL KTQLEQRIM SDHQHNLKN 
      KVEPKVEME VQKTENKLQ KFGIRVLPP EKSPKSAEL 
      SQNENNLNL EKLPIGLDE VDDKKPQVK KRERKLTPE 
      IFDRDHVNN SSGIKRDAN GIPQEIPNY MMNAAVAAR 
      SNRKSSVKD EEDKTPKKT KGKAPSPPE FKNLDEIQP 
      VDDFEEIAP KPTQDDLVE EIEVELRNK TMKDYNSDS 
      DLETDNHSS VNTIELNSS DITIHHAED TEDRQNRRT 
      ASTGDLTKI QRTRKSSTG TLERAQSLD ITDTSIPTL 
      TKKRKGRIE DDLMASEED IFGKVVMSK EPRLSLMLD 
      GLNTFQRNR LKKSTEWGN LEDAILKLN QEDESDPKT 
      DLDLSGKTE EIDAVVNKI NEIKRESLE VQPPIKEET 
      PPPKPEKKV KNQIWPFET KANGSAIVD DNPRLASVE 
      KRQRKPPIL DPVKPAVPE KKDHLLAKK ILQNFIFTE 
      RIACSQDDD DTNVSDDIK VSRHSLGSL ERAKSDDAK 
      INVSNVTVN NLRPQSEEN LHSLEIDTT EPELYTTAL 
      DSTVKPEPE TPKVTISTP DIIKNVTIA EAINKLNND 
      VMVQGPSSL TLEISNDAE IKDNRFMVD VKPDRVKKE 
      DSGSETKSF EDDSSKSNS SLTYITEIQ VLTPNNSTS 
      TNISEIEII PNVNSNSNG KTLDLEHEF EDYVRNFES 
      NVKTFESNI QKFENHLDS TPKPDLKSI IITENDAEK 
      ELHKIQEIA EEQLKKLPE MRFTTSSYE PSKLPEKRQ 
      SQIELLRSN FEKSPPKSK PDTPKSRIP IATTAKTPP 
      TSPERRDSR NLDMENDKD ILELMSSTV HSTPKIKPP 
      KNVTVTSIR SNSKIPSGL PVLGGRPAV PPRRQEEDH 
      VVQVSTNGG VESSFKQWV FNPTDAVTN IVVAESKQQ 
      K
    CDS sequence
    • >TC002828 RA
      atggcaccg cattctcgc agtttgtgg ttggtgctg 
      gtgctcagg gccgggctg gccagcgct caggaggcc 
      gcctgttac ggatctgga tccatcgcg ctctcggtc 
      atcctgtcc gtcgtcttc acggccgtt ctgttggcg 
      gcgctgctc tacctctgg gggaagtac cggaaacgg 
      aaggagctc atcctcgaa accgacccc gagaaaggc 
      aaaggcggc gaatacgca ttcgacaat ccagcgttc 
      aaagatcct ggactaact ccgatcggg aaattggag 
      aaggaaaag gtcgacgcg aacaagacg aaatggacg 
      cagtggtcg cccctgacg gccctcacc accaagtcc 
      gagaaaaag aagactttg gatgacagt gccattgat 
      ggaaatttg gtgaaagta gtggcgtta cgcagccat 
      gatttcacc ggactgggg ttcaacatt tgtggcaac 
      atgaaagaa gggatttac atcaaggat gtcctgcac 
      cgagggcct gcctccgag tcaggcaaa ctcaatccc 
      ggtgataga atcaacagt gtcaccatt agtttcgaa 
      cacatggta tacgaagat gctttaaca attttaagt 
      tatgctagt ccctatgaa gtcataatc gaagcacga 
      ggcgggaaa atgttccaa acaagtggg caaggggcc 
      caaccaagt caccccgtg tacaggagc tcctcttgt 
      accgaactc ttccaagtt aactccgcc aaacgtaaa 
      ttatttggc accgaagaa cactccggt tccctcagt 
      tcgaattac tcaagtctc cagaaatct agaagtaac 
      atgacgacc cttgaacgc aaggaatcc gaatctccc 
      cgaaaccgc cccaagaaa aaccccccg aagacacaa 
      atgtccccc gaacagtta aaaacgcag cttgagcaa 
      agaataatg tcagaccac caacacaac ttgaaaaac 
      aaagtcgag ccaaaagtc gaaatggag gtccaaaaa 
      accgagaac aagttgcaa aaattcggc attagggtc 
      ctcccgcct gagaagagc cccaaatcg gccgagctg 
      tcccaaaac gagaacaac ctcaacttg gagaaactc 
      cccatcgga cttgacgaa gtcgacgat aagaaacca 
      caagtcaaa aagcgggaa cgcaaacta acccccgaa 
      attttcgat cgggaccac gtaaacaac agttccggg 
      atcaaacgg gacgccaac ggaatcccc caggagatc 
      cccaactac atgatgaac gcggccgtt gccgcccgc 
      agtaaccgc aaaagctcg gtcaaagac gaggaggac 
      aaaaccccc aagaaaacc aaaggcaag gcgccgtca 
      cctcccgaa ttcaaaaac ctggatgag atacaacca 
      gttgacgat ttcgaggaa atcgctccc aagcccacc 
      caagacgac ttagttgag gagattgag gtcgagttg 
      aggaacaaa acaatgaaa gactacaac tcggactcg 
      gatctcgaa accgacaat cactcctct gtcaacacc 
      atcgagctg aattcgagc gacatcacc atccaccac 
      gccgaagac accgaagac cgccaaaac cgccgaact 
      gcaagcacc ggcgacttg accaagatc cagcgcacg 
      cggaaatcc agcactggc accctggag cgcgcccag 
      agcctggac atcaccgac accagcata ccaaccctg 
      accaagaag cgcaaaggg cggatcgag gacgacttg 
      atggcttcc gaggaggat attttcgga aaagtggtc 
      atgagtaaa gaaccccgc ctgtcgctg atgctcgac 
      ggcttgaac acgttccag cgaaaccgc ctgaagaag 
      tcaaccgag tgggggaac ctcgaggac gccatcctc 
      aagctgaac caagaggac gaaagcgac ccgaagacc 
      gacctggac ctcagcggc aagaccgag gaaatcgac 
      gctgttgtg aacaaaatc aacgaaatc aagcgcgag 
      tccctggag gtgcagccg cccatcaag gaggaaacc 
      ccgccgcct aagcccgag aagaaggtc aagaaccag 
      atctggccg ttcgagacc aaggcgaac ggaagtgcc 
      atagtggac gataacccg cggcttgcc tcggtggag 
      aagcggcag cgcaagccc ccgattttg gacccagtg 
      aagccggcg gttcccgaa aagaaggac cacctcctg 
      gcgaaaaaa atcctccag aattttatc ttcacggag 
      aggatcgcc tgcagtcag gacgacgac gatacgaac 
      gtctccgac gatattaag gtctccagg cactccttg 
      gggagcctg gagcgggca aagtccgac gacgccaag 
      ataaacgtt agcaatgtc actgttaat aatctccga 
      ccgcaaagc gaggagaat ctgcacagt ttggaaatt 
      gacacgact gagcccgag ttgtacacc acggcgctt 
      gatagtacg gttaaaccg gagcccgaa acgcccaaa 
      gtcacgata agtaccccc gacatcatc aaaaatgtc 
      acaatcgcc gaagcgata aataagtta aacaacgat 
      gtgatggtt cagggaccg tcgtcactg actttggag 
      atttcgaac gatgcggaa atcaaggac aatcgattt 
      atggtcgat gttaaaccg gatcgagtg aagaaggaa 
      gattcggga agcgagacg aagagtttt gaggacgat 
      agtagcaaa agtaactcg tccctgact tacatcacc 
      gagatccag gtcttgact cctaacaac tcgaccagt 
      accaacatt tctgagatc gaaatcatc cccaatgtt 
      aacagcaat agcaatggc aaaaccctg gacctggag 
      cacgaattc gaggactac gtccgcaac ttcgaatcc 
      aacgtgaaa accttcgag tccaacatc caaaagttt 
      gaaaaccac ctggactcg accccgaaa cccgacttg 
      aagagcatc ataataacg gaaaacgac gccgaaaag 
      gaactccac aaaatccaa gaaatcgca gaggagcaa 
      ctgaagaaa ctccccgaa atgcgcttc acaacatcc 
      agctacgag ccatcgaaa ctcccggag aagcgccag 
      tcgcagatc gagctcctg cgttcaaac ttcgagaaa 
      agcccgccc aagtccaag ccagacacg cccaagtcg 
      cgcattccc atcgcgacc acggccaaa actccgccc 
      acctcgccg gaaaggcgc gattcgcgc aatctggac 
      atggaaaac gacaaggac atcctcgag ttgatgtcg 
      tcgacggtc cactccacc cccaagata aaaccgccc 
      aaaaacgtc acagtcact tcgattcgg agcaattcg 
      aagattccg tccggactg cccgttttg ggcgggagg 
      ccggcggtg ccgccccgt aggcaggag gaagaccac 
      gtggtgcag gtctcgacc aatggcgga gtggagagt 
      agttttaag cagtgggtg tttaatccc accgatgcc 
      gtgacgaat atcgtcgtg gctgagagt aaacagcag 
      aagtga
    mRNA sequence
    • >TC002828 RA
      agtgcccct atcccgccc cctttatct gcaactggg 
      ggcaatccc ttgcaataa ataactacc cccttccac 
      tggccccaa gcacccctc ggccacatc cggaaaacc 
      cccaattgc ggtcggaat gcgcccccc tcggcccat 
      cttcctcgc cgcaataat cagacaggt ttcggtccc 
      agtcggtca ttcaaggta gtaagttca aagatagcc 
      tcaggtgca cacacacac atacacaca cctgccagg 
      cctccgagt cggtgcctc acagatagt cgcatgttc 
      gtccacctg tcgggtgta aacaaagta gtccgtggt 
      gcagtgtga tcgtcacat gtttttccc ggttttcgc 
      ttgtttcgg gatgggtga tggtgctgt gctgggatt 
      aagggacac ggattaggg acactggga ccaaaccgc 
      aatgaagaa cggccgttg agaacctcc gtgaaaaac 
      gttcgaaat ggcaccgca ttctcgcag tttgtggtt 
      ggtgctggt gctcagggc cgggctggc cagcgctca 
      ggaggccgc ctgttacgg atctggatc catcgcgct 
      ctcggtcat cctgtccgt cgtcttcac ggccgttct 
      gttggcggc gctgctcta cctctgggg gaagtaccg 
      gaaacggaa ggagctcat cctcgaaac cgaccccga 
      gaaaggcaa aggcggcga atacgcatt cgacaatcc 
      agcgttcaa agatcctgg actaactcc gatcgggaa 
      attggagaa ggaaaaggt cgacgcgaa caagacgaa 
      atggacgca gtggtcgcc cctgacggc cctcaccac 
      caagtccga gaaaaagaa gactttgga tgacagtgc 
      cattgatgg aaatttggt gaaagtagt ggcgttacg 
      cagccatga tttcaccgg actggggtt caacatttg 
      tggcaacat gaaagaagg gatttacat caaggatgt 
      cctgcaccg agggcctgc ctccgagtc aggcaaact 
      caatcccgg tgatagaat caacagtgt caccattag 
      tttcgaaca catggtata cgaagatgc tttaacaat 
      tttaagtta tgctagtcc ctatgaagt cataatcga 
      agcacgagg cgggaaaat gttccaaac aagtgggca 
      aggggccca accaagtca ccccgtgta caggagctc 
      ctcttgtac cgaactctt ccaagttaa ctccgccaa 
      acgtaaatt atttggcac cgaagaaca ctccggttc 
      cctcagttc gaattactc aagtctcca gaaatctag 
      aagtaacat gacgaccct tgaacgcaa ggaatccga 
      atctccccg aaaccgccc caagaaaaa ccccccgaa 
      gacacaaat gtcccccga acagttaaa aacgcagct 
      tgagcaaag aataatgtc agaccacca acacaactt 
      gaaaaacaa agtcgagcc aaaagtcga aatggaggt 
      ccaaaaaac cgagaacaa gttgcaaaa attcggcat 
      tagggtcct cccgcctga gaagagccc caaatcggc 
      cgagctgtc ccaaaacga gaacaacct caacttgga 
      gaaactccc catcggact tgacgaagt cgacgataa 
      gaaaccaca agtcaaaaa gcgggaacg caaactaac 
      ccccgaaat tttcgatcg ggaccacgt aaacaacag 
      ttccgggat caaacggga cgccaacgg aatccccca 
      ggagatccc caactacat gatgaacgc ggccgttgc 
      cgcccgcag taaccgcaa aagctcggt caaagacga 
      ggaggacaa aacccccaa gaaaaccaa aggcaaggc 
      gccgtcacc tcccgaatt caaaaacct ggatgagat 
      acaaccagt tgacgattt cgaggaaat cgctcccaa 
      gcccaccca agacgactt agttgagga gattgaggt 
      cgagttgag gaacaaaac aatgaaaga ctacaactc 
      ggactcgga tctcgaaac cgacaatca ctcctctgt 
      caacaccat cgagctgaa ttcgagcga catcaccat 
      ccaccacgc cgaagacac cgaagaccg ccaaaaccg 
      ccgaactgc aagcaccgg cgacttgac caagatcca 
      gcgcacgcg gaaatccag cactggcac cctggagcg 
      cgcccagag cctggacat caccgacac cagcatacc 
      aaccctgac caagaagcg caaagggcg gatcgagga 
      cgacttgat ggcttccga ggaggatat tttcggaaa 
      agtggtcat gagtaaaga accccgcct gtcgctgat 
      gctcgacgg cttgaacac gttccagcg aaaccgcct 
      gaagaagtc aaccgagtg ggggaacct cgaggacgc 
      catcctcaa gctgaacca agaggacga aagcgaccc 
      gaagaccga cctggacct cagcggcaa gaccgagga 
      aatcgacgc tgttgtgaa caaaatcaa cgaaatcaa 
      gcgcgagtc cctggaggt gcagccgcc catcaagga 
      ggaaacccc gccgcctaa gcccgagaa gaaggtcaa 
      gaaccagat ctggccgtt cgagaccaa ggcgaacgg 
      aagtgccat agtggacga taacccgcg gcttgcctc 
      ggtggagaa gcggcagcg caagccccc gattttgga 
      cccagtgaa gccggcggt tcccgaaaa gaaggacca 
      cctcctggc gaaaaaaat cctccagaa ttttatctt 
      cacggagag gatcgcctg cagtcagga cgacgacga 
      tacgaacgt ctccgacga tattaaggt ctccaggca 
      ctccttggg gagcctgga gcgggcaaa gtccgacga 
      cgccaagat aaacgttag caatgtcac tgttaataa 
      tctccgacc gcaaagcga ggagaatct gcacagttt 
      ggaaattga cacgactga gcccgagtt gtacaccac 
      ggcgcttga tagtacggt taaaccgga gcccgaaac 
      gcccaaagt cacgataag tacccccga catcatcaa 
      aaatgtcac aatcgccga agcgataaa taagttaaa 
      caacgatgt gatggttca gggaccgtc gtcactgac 
      tttggagat ttcgaacga tgcggaaat caaggacaa 
      tcgatttat ggtcgatgt taaaccgga tcgagtgaa 
      gaaggaaga ttcgggaag cgagacgaa gagttttga 
      ggacgatag tagcaaaag taactcgtc cctgactta 
      catcaccga gatccaggt cttgactcc taacaactc 
      gaccagtac caacatttc tgagatcga aatcatccc 
      caatgttaa cagcaatag caatggcaa aaccctgga 
      cctggagca cgaattcga ggactacgt ccgcaactt 
      cgaatccaa cgtgaaaac cttcgagtc caacatcca 
      aaagtttga aaaccacct ggactcgac cccgaaacc 
      cgacttgaa gagcatcat aataacgga aaacgacgc 
      cgaaaagga actccacaa aatccaaga aatcgcaga 
      ggagcaact gaagaaact ccccgaaat gcgcttcac 
      aacatccag ctacgagcc atcgaaact cccggagaa 
      gcgccagtc gcagatcga gctcctgcg ttcaaactt 
      cgagaaaag cccgcccaa gtccaagcc agacacgcc 
      caagtcgcg cattcccat cgcgaccac ggccaaaac 
      tccgcccac ctcgccgga aaggcgcga ttcgcgcaa 
      tctggacat ggaaaacga caaggacat cctcgagtt 
      gatgtcgtc gacggtcca ctccacccc caagataaa 
      accgcccaa aaacgtcac agtcacttc gattcggag 
      caattcgaa gattccgtc cggactgcc cgttttggg 
      cgggaggcc ggcggtgcc gccccgtag gcaggagga 
      agaccacgt ggtgcaggt ctcgaccaa tggcggagt 
      ggagagtag ttttaagca gtgggtgtt taatcccac 
      cgatgccgt gacgaatat cgtcgtggc tgagagtaa 
      acagcagaa gtgatgata cgtgcaatg gaagaggcg 
      gctcaattt agtgtagtg gaattattt atataaaaa 
      cgtagttgt atatatttg taaaagtaa tgtttatgt 
      atgtgctat tattttaag ctgatgatt ttgttggtt 
      tattttgta attttaatc gagggtgac tatacatta 
      ataacaaaa agtacatgt ttttctaac cagagatag 
      atatatcca ataaatgaa attaaaccc a
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