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Gene information
General information
TC002817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9128217..9131863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002817
    iBeetle screen iB_00448

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002817 RA
      MASLLRQFT RTLRSPVAR NGVCATAIR FKHSEAAPA 
      EETRHTVRK PDLLARSQL KDFGKYCAE CLPKYIQKV 
      QITAGDELE LLIVPEGVL PVLQFLKDH HNAQFANIT 
      DIAGMDVPS REYRFEVIY NLLSLRFNS RIRVKTYTD 
      ELTPLDSAC DIYKGANWY EREIWDMYG VFFANHPDL 
      RRILTDYGF EGHPFRKDF PLSGYVEVR YDDEKKRVV 
      VEPLELAQE FRKFELSAP WEQFPNFRN APPTAEEVP 
      VEEKK
    CDS sequence
    • >TC002817 RA
      atggcttcc cttttgaga caattcaca aggaccttg 
      cggtcaccc gtggcccgc aatggggtc tgtgccacc 
      gctatcaga ttcaaacat tcggaagca gcccctgca 
      gaagagaca cgccatacg gtcagaaag ccggacctt 
      ttggcccgc tcccagctg aaagacttt ggaaaatac 
      tgcgctgaa tgccttcca aagtacatc cagaaggtg 
      caaatcact gctggcgac gagctcgag ctgttgata 
      gtccccgag ggggtgctc cctgtgctg cagttcctc 
      aaagaccat cacaatgcc cagtttgcc aacataacg 
      gatatcgca ggcatggat gtcccgagt cgcgagtac 
      cgctttgag gtgatttac aacctcctg tctttgcgc 
      ttcaactcc cggattaga gtcaagacg tacactgac 
      gagttgacg ccgcttgac tccgcctgc gatatctac 
      aagggggcc aactggtac gagcgcgag atttgggac 
      atgtatggg gtgtttttc gcaaatcac cccgatttg 
      aggcggatt ctcaccgat tacgggttc gaggggcat 
      ccgttcagg aaggacttc cccttgagc ggctatgtc 
      gaggtgagg tacgacgat gagaaaaag cgcgtggtg 
      gttgaaccg ctcgagttg gctcaggag ttcaggaag 
      tttgagctg tcggccccc tgggagcag ttccccaac 
      ttcaggaat gcgcccccc actgccgag gaggttcca 
      gtagaggag aagaagtga
    mRNA sequence
    • >TC002817 RA
      cggtaaagg attatttct ttcaaacaa atttgtatt 
      atttaactt aaccaaaga caatatatc gtataattg 
      cgaaagcaa gttaaaggc cttattttt tagtatttt 
      ttgaaaaaa atttacaag ggcaatgct tcgccaaca 
      taacgggct gaaatgtca ctagcggtg tgaggttag 
      gggtgttat taggtaaca aataaataa aaatttgat 
      ggcttccct tttgagaca attcacaag gaccttgcg 
      gtcacccgt ggcccgcaa tggggtctg tgccaccgc 
      tatcagatt caaacattc ggaagcagc ccctgcaga 
      agagacacg ccatacggt cagaaagcc ggacctttt 
      ggcccgctc ccagctgaa agactttgg aaaatactg 
      cgctgaatg ccttccaaa gtacatcca gaaggtgca 
      aatcactgc tggcgacga gctcgagct gttgatagt 
      ccccgaggg ggtgctccc tgtgctgca gttcctcaa 
      agaccatca caatgccca gtttgccaa cataacgga 
      tatcgcagg catggatgt cccgagtcg cgagtaccg 
      ctttgaggt gatttacaa cctcctgtc tttgcgctt 
      caactcccg gattagagt caagacgta cactgacga 
      gttgacgcc gcttgactc cgcctgcga tatctacaa 
      gggggccaa ctggtacga gcgcgagat ttgggacat 
      gtatggggt gtttttcgc aaatcaccc cgatttgag 
      gcggattct caccgatta cgggttcga ggggcatcc 
      gttcaggaa ggacttccc cttgagcgg ctatgtcga 
      ggtgaggta cgacgatga gaaaaagcg cgtggtggt 
      tgaaccgct cgagttggc tcaggagtt caggaagtt 
      tgagctgtc ggccccctg ggagcagtt ccccaactt 
      caggaatgc gccccccac tgccgagga ggttccagt 
      agaggagaa gaagtgatt aggtaaatg ttaaataat 
      aaagttgtg ttttatgca aagacttta tttgtttcc 
      taacaatgt ttccgtcat ttgtactgt agcaaaaca 
      acagtcggt tttttttcc tcataaaat ataatttca 
      aagcaccaa attacaata atcaaaaat gggttacca 
      agtttgcta tgttaaatt gacttgtca tgaatactg 
      tcataaaga aaaacatgt gggcatcac atgtcagaa 
      ccaatttca atgacagtg tgtacaaaa gttgatcaa 
      caaattccc ctattgaca tttttattt cattagaac 
      cccgtcaac attttaaaa tgcgaactc tagaataaa 
      tatgtcaga ataaataat aatacaaaa aataacgtt 
      ggctaacat aatattatt tacttaaat acaatttgt 
      gttaacttg gcaattttc tgtactgag agggtcgcg 
      acaacccgc ttagaacta caattagtc attattggt 
      aatactaac aatatttca caagtacag gtaccatct 
      atcacactt ttctaacat caatggtaa cttcgttac 
      atccattta tttacaaga agcttccaa caacacgaa 
      actcactcc gcagtcaaa aaacaatta ccgcgcagc 
      gaattttcg tattgtttc gtatcttat ctgattttt 
      ttaggaaaa tacctccaa aatataaac atacaaaac 
      aaattacaa ctgtacaag ttaaaaata tacttaaca 
      tttaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY