Status
Please wait ...
Gene information
General information
TC002805 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9628082..9634509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002805
    iBeetle screen iB_00447

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002805 RA
      MHASAPRKS IVWAYVWWL FGGIFGLHL FYLERDAHA 
      FLTWSTLGG YGLGWLADV TKIPRYVRD CNDDPKFLE 
      ELGERMRKN RKPPFSTSR FISAVMVGY LWGQLVIMA 
      IPEDEVWGF NWKIFHWVI PFAVALGVW VVGNIGREK 
      GKPWLTIFA SYSSYLTRW YFPDENIWL TVMVFCAAL 
      TFDTFSKEW RRTPRKKKT FIRRITVLI ICAMLYVAL 
      WSSYFYFNG KITDANGDE IPVHEAIHH FFTSPWWTD 
      LKQSLYDTY QYAQHHGWY EIWRQIIEL SDPQGEQNS 
      YKVLGVGPS SSQSEITAK WRALSREWH PDKVKDPEQ 
      RQAAQDKFM EIQQAYEIL SNIKSKRKR KNKKSVAND
    CDS sequence
    • >TC002805 RA
      atgcacgct tctgctcct agaaaaagc atcgtttgg 
      gcgtacgtc tggtggctg ttcggaggc attttcggc 
      ctccatttg ttctatctc gaaagggat gcgcacgcc 
      ttcctcacc tggagcacc ctcggaggc tacggcctc 
      ggatggctc gctgatgtt accaaaatc ccgagatac 
      gtgcgggat tgcaacgat gatcccaag tttttggag 
      gagttgggc gaaaggatg cggaaaaac agaaagccc 
      ccgttttca acgagcaga ttcatctcc gccgtaatg 
      gtgggctac ctctggggc cagttggtc ataatggcc 
      attccagaa gacgaagtc tggggattt aactggaaa 
      atcttccat tgggtgatt ccctttgca gtcgcactt 
      ggtgtgtgg gttgtggga aacattgga agagagaag 
      ggcaagccc tggctcaca attttcgcc tcgtattcg 
      agttatctg acgcgttgg tacttcccc gacgagaac 
      atttggctc acagtgatg gtgttttgt gccgcattg 
      actttcgac acgttttcc aaagagtgg cgacgaact 
      ccgcgcaag aaaaaaacc ttcattcgc cgaattacc 
      gtccttata atttgcgca atgttgtat gtggcgctt 
      tggtcgtcc tatttctac tttaatgga aaaatcacc 
      gacgccaac ggcgacgaa attcccgtc cacgaagcc 
      atacatcac ttcttcacc tcgccctgg tggaccgac 
      ctcaagcag tcgctttac gacacctat caatacgca 
      cagcatcac ggatggtac gagatttgg aggcaaatt 
      atcgaacta tcggacccg caaggggag cagaactcg 
      tataaggtg ctgggagtg gggccttcg tccagtcag 
      agcgaaatc accgccaaa tggcgggct ctgagcagg 
      gaatggcat cctgataaa gtcaaggat cctgagcag 
      cgtcaggcg gcgcaggac aagtttatg gagattcag 
      caagcttat gagattctg tccaatatt aagagcaag 
      aggaagagg aaaaataaa aagtctgtt gccaatgat 
      tga
    mRNA sequence
    • >TC002805 RA
      cgataccac aaaacggaa gaggcccac tttttgacc 
      cgattcctc gccgcttac ctatatttc ggcctttgc 
      cgtacgaaa gtaccgtaa tattatgaa acgttgcct 
      acctgaagc gacccaaca acgtgaagc tacgtggac 
      atcctactc ctacctggt agaagtaag ttagtttct 
      ttagtttga tatttctgt ttgcggcgt gcgttcaat 
      gaacttgcg gtgtaataa tgcaaattt ccaagtgcg 
      tttcaaaaa gccgatcca aatggtgac ataattgcg 
      cgaaaaaac acttatggc agtggtgat atgtagcga 
      aaagtccca aataactga tatgtgata tgtttgtgt 
      tatccgaac gtgttttgt taagtcatt agttttcct 
      aacattaaa atgcacgct tctgctcct agaaaaagc 
      atcgtttgg gcgtacgtc tggtggctg ttcggaggc 
      attttcggc ctccatttg ttctatctc gaaagggat 
      gcgcacgcc ttcctcacc tggagcacc ctcggaggc 
      tacggcctc ggatggctc gctgatgtt accaaaatc 
      ccgagatac gtgcgggat tgcaacgat gatcccaag 
      tttttggag gagttgggc gaaaggatg cggaaaaac 
      agaaagccc ccgttttca acgagcaga ttcatctcc 
      gccgtaatg gtgggctac ctctggggc cagttggtc 
      ataatggcc attccagaa gacgaagtc tggggattt 
      aactggaaa atcttccat tgggtgatt ccctttgca 
      gtcgcactt ggtgtgtgg gttgtggga aacattgga 
      agagagaag ggcaagccc tggctcaca attttcgcc 
      tcgtattcg agttatctg acgcgttgg tacttcccc 
      gacgagaac atttggctc acagtgatg gtgttttgt 
      gccgcattg actttcgac acgttttcc aaagagtgg 
      cgacgaact ccgcgcaag aaaaaaacc ttcattcgc 
      cgaattacc gtccttata atttgcgca atgttgtat 
      gtggcgctt tggtcgtcc tatttctac tttaatgga 
      aaaatcacc gacgccaac ggcgacgaa attcccgtc 
      cacgaagcc atacatcac ttcttcacc tcgccctgg 
      tggaccgac ctcaagcag tcgctttac gacacctat 
      caatacgca cagcatcac ggatggtac gagatttgg 
      aggcaaatt atcgaacta tcggacccg caaggggag 
      cagaactcg tataaggtg ctgggagtg gggccttcg 
      tccagtcag agcgaaatc accgccaaa tggcgggct 
      ctgagcagg gaatggcat cctgataaa gtcaaggat 
      cctgagcag cgtcaggcg gcgcaggac aagtttatg 
      gagattcag caagcttat gagattctg tccaatatt 
      aagagcaag aggaagagg aaaaataaa aagtctgtt 
      gccaatgat tgatagtgc tagttgcaa tttattgtt 
      atttaatat aactgtgtt ttatcattt gagatttta 
      ttagattaa agattgagc gaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY