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Gene information
General information
TC002792 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10234328..10241470
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002792
    iBeetle screen iB_00446

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002792 RA
      MAVKQKVVE CVAQSAEAR LQLILRTLQ DLTAQGLQD 
      KKCANIVSL LGTIHVPTD PSDYKQFIG SIKSVQEIL 
      TKECMKNDL VYLTLRSLY HEISTSQKP LSPAMSIVL 
      TLIQVDYIP TAVDWILQT GYSPENHKK ALMTLCNWL 
      PKWTWTENL GRLVLTFMQ GLEAQQQLE IVFEVTEAT 
      IEPLFRLLI LPEHRKSVG PIVKYMLFS MLSQSNPVS 
      FDKVVPHVG SVVAYLYKE KSDSSLEYF GDIVKVCFL 
      LMDHFSDYS NRYVQLKKD LEQHLPAAN CIENGPSNS 
      VATVYSASG KVGLNNLGN TCYMNSVLQ ALFMTKPFR 
      NEILLYNKD MTSLLSKLQ TLFALLQHS KRASISPHD 
      ISNLARPPG FLPGHQHDS SEFLGYLLD VLHEQEKTA 
      VCGNGTKAD HVPPSYNTI VQSAFGGRT VSVSRCGEC 
      DTKSERVDN FWELQLSFP NTTDNQSVQ TLLNYYLQP 
      EKLSGDNQY HCEVCDCLT DGERVTCIE EAPPRLILT 
      LKHFRYDQA SQQRTKLLQ SIKLDNHVQ LDTGLYVLY 
      AAVVHCGSS VDSGHYYTF ARDGDEWFK FNDCSVMRT 
      MAEELCSLK PPETPYILF YSRQDCSDP EALPRTVLS 
      QRLQLVINN DHTEFELEK RRRPIKTYN SKQNRNDEP 
      PPPGCGGPG FSSNSSNMY VC
    CDS sequence
    • >TC002792 RA
      atggcagtg aagcaaaag gtggtggaa tgcgtggcc 
      cagagcgcg gaggcgcgc ctccagctc atcctgcgc 
      accctgcag gacctcacg gcccagggg ctccaggac 
      aagaagtgc gccaacatc gtcagcctc ctggggacc 
      atccacgtg cccacggac cccagcgac tacaagcag 
      ttcatcggg agcattaag agcgtccag gagatcctc 
      accaaagaa tgcatgaag aacgacttg gtctacctg 
      accttgagg tcgctttac cacgaaatc tccacgtct 
      cagaagccg ctctcgccg gccatgtcc atagttttg 
      acccttatc caagtcgat tatatcccg acggccgtg 
      gactggatc ctccaaacg gggtattcc cccgaaaac 
      cacaaaaaa gccctcatg acgctgtgt aattggctg 
      ccgaaatgg acctggacg gagaacctc ggccggttg 
      gtcctcacc ttcatgcag ggcctggag gcccagcaa 
      cagctcgag atcgtgttc gaggtcacg gaggccacg 
      attgagccc cttttcagg ctgttgatt ttgcccgag 
      caccgcaaa agcgtgggc cccattgtc aagtacatg 
      ctgttctcc atgttgtcg caaagcaac cccgtgtcc 
      ttcgacaag gtcgtcccc cacgtgggg agcgtcgtc 
      gcctacttg tacaaagaa aagtccgat tctagtctt 
      gaatatttc ggcgacatc gtcaaagtg tgttttctg 
      ttaatggac cacttctcc gactattca aatcgctac 
      gtccagttg aagaaggac ctggagcag catcttcct 
      gcggcgaat tgtatcgag aacgggccg tcgaattcc 
      gtggcgacc gtttattca gcgtcgggg aaggtgggg 
      ttgaacaac ctggggaac acctgttac atgaacagt 
      gtcctccag gcgttgttt atgacgaag ccgttccgc 
      aatgagatt cttttgtat aataaggat atgacgtcg 
      ttgttgtcc aagttgcag acgctgttt gcgctgttg 
      caacactcg aagagggct tccatctcg ccgcatgat 
      atttcgaat ttggcgagg ccgcccggg tttttgcca 
      gggcatcag catgacagt tcggagttt ttggggtat 
      ttgttggat gttttgcac gagcaggag aagacggcc 
      gtttgtggg aatggtacc aaagcagac catgtgccc 
      ccctcatac aacaccata gtccagtca gccttcggc 
      ggtcgcacg gtgtcggtt tcgcgttgc ggcgaatgc 
      gacacaaaa agcgaacgc gtcgacaac ttctgggag 
      ctgcaactc tccttcccc aacaccacc gacaatcag 
      tccgtgcag accctcctc aactactac ctccaaccg 
      gagaagctc agcggcgac aaccaatac cactgcgag 
      gtgtgcgac tgtctcacc gacggcgag cgcgtcacc 
      tgcatcgaa gaggctccc ccacgtctc atcctcacc 
      ctgaagcac ttccgctac gaccaggcc tcccagcag 
      cgcaccaaa ctcctccag agcatcaaa ctcgacaat 
      cacgtgcag ctcgacacc ggcctctac gtgctctac 
      gcggcggtg gtccactgc ggctccagt gtggactcg 
      gggcactat tacacgttc gcacgagac ggagacgag 
      tggttcaag ttcaacgat tgttccgtt atgaggact 
      atggcggag gaactttgc agtttgaag ccgccagaa 
      acgccgtat attttgttt tatagtcgg caggactgc 
      tcggatccc gaggcgctg ccgaggact gtcttgtcg 
      cagagatta cagttagtg attaataat gatcacact 
      gagttcgag cttgagaag agaaggagg ccgatcaag 
      acgtacaac agcaaacaa aatcggaac gatgaaccg 
      ccgcctccg gggtgtgga gggcctggg ttttctagt 
      aattcatct aatatgtat gtatgctaa
    mRNA sequence
    • >TC002792 RA
      ggccattta attatgatt cccgttttt gccgtttaa 
      ttaatttct ttttgtgtt ttatttatt ttgctatca 
      tcacccttc gaaaaatgc gtttgtaag tcttaacga 
      acgcccaaa gtgtgccta aaccaagtt cctccaccg 
      cagctacga ttccaacga ccgtcgaaa aaccctcac 
      ctggatgac attcattgc tatcatctc ccagtaaga 
      aacatggca gtgaagcaa aaggtggtg gaatgcgtg 
      gcccagagc gcggaggcg cgcctccag ctcatcctg 
      cgcaccctg caggacctc acggcccag gggctccag 
      gacaagaag tgcgccaac atcgtcagc ctcctgggg 
      accatccac gtgcccacg gaccccagc gactacaag 
      cagttcatc gggagcatt aagagcgtc caggagatc 
      ctcaccaaa gaatgcatg aagaacgac ttggtctac 
      ctgaccttg aggtcgctt taccacgaa atctccacg 
      tctcagaag ccgctctcg ccggccatg tccatagtt 
      ttgaccctt atccaagtc gattatatc ccgacggcc 
      gtggactgg atcctccaa acggggtat tcccccgaa 
      aaccacaaa aaagccctc atgacgctg tgtaattgg 
      ctgccgaaa tggacctgg acggagaac ctcggccgg 
      ttggtcctc accttcatg cagggcctg gaggcccag 
      caacagctc gagatcgtg ttcgaggtc acggaggcc 
      acgattgag ccccttttc aggctgttg attttgccc 
      gagcaccgc aaaagcgtg ggccccatt gtcaagtac 
      atgctgttc tccatgttg tcgcaaagc aaccccgtg 
      tccttcgac aaggtcgtc ccccacgtg gggagcgtc 
      gtcgcctac ttgtacaaa gaaaagtcc gattctagt 
      cttgaatat ttcggcgac atcgtcaaa gtgtgtttt 
      ctgttaatg gaccacttc tccgactat tcaaatcgc 
      tacgtccag ttgaagaag gacctggag cagcatctt 
      cctgcggcg aattgtatc gagaacggg ccgtcgaat 
      tccgtggcg accgtttat tcagcgtcg gggaaggtg 
      gggttgaac aacctgggg aacacctgt tacatgaac 
      agtgtcctc caggcgttg tttatgacg aagccgttc 
      cgcaatgag attcttttg tataataag gatatgacg 
      tcgttgttg tccaagttg cagacgctg tttgcgctg 
      ttgcaacac tcgaagagg gcttccatc tcgccgcat 
      gatatttcg aatttggcg aggccgccc gggtttttg 
      ccagggcat cagcatgac agttcggag tttttgggg 
      tatttgttg gatgttttg cacgagcag gagaagacg 
      gccgtttgt gggaatggt accaaagca gaccatgtg 
      cccccctca tacaacacc atagtccag tcagccttc 
      ggcggtcgc acggtgtcg gtttcgcgt tgcggcgaa 
      tgcgacaca aaaagcgaa cgcgtcgac aacttctgg 
      gagctgcaa ctctccttc cccaacacc accgacaat 
      cagtccgtg cagaccctc ctcaactac tacctccaa 
      ccggagaag ctcagcggc gacaaccaa taccactgc 
      gaggtgtgc gactgtctc accgacggc gagcgcgtc 
      acctgcatc gaagaggct cccccacgt ctcatcctc 
      accctgaag cacttccgc tacgaccag gcctcccag 
      cagcgcacc aaactcctc cagagcatc aaactcgac 
      aatcacgtg cagctcgac accggcctc tacgtgctc 
      tacgcggcg gtggtccac tgcggctcc agtgtggac 
      tcggggcac tattacacg ttcgcacga gacggagac 
      gagtggttc aagttcaac gattgttcc gttatgagg 
      actatggcg gaggaactt tgcagtttg aagccgcca 
      gaaacgccg tatattttg ttttatagt cggcaggac 
      tgctcggat cccgaggcg ctgccgagg actgtcttg 
      tcgcagaga ttacagtta gtgattaat aatgatcac 
      actgagttc gagcttgag aagagaagg aggccgatc 
      aagacgtac aacagcaaa caaaatcgg aacgatgaa 
      ccgccgcct ccggggtgt ggagggcct gggttttct 
      agtaattca tctaatatg tatgtatgc taaatgttt 
      caatatata tcggatttg ggttattac atagttgaa 
      ttctttcta atatgttga acaacgaac tgaactcat 
      ctgttggca atttggcga tagggtcag gtggggtaa 
      gtgttaact aggggtctc aattaatat tcggaatac 
      agttgggat gattgattt catagtgtg ccccacccg 
      accctggtt attttgccg gtggataac ttttcgccc 
      agtgacata tacgattgt aaatattag ttttattca 
      cactcacac ataaatagg aattgtttc aaaaatagt 
      cacggagta ttgttgttt tattgttgt ttttgtttt 
      attttgaaa gtacaacgt actgaattg ttttttttt 
      cgaaatcgt gattaattt gctttccat caattgtct 
      aggtttgtt tacacttat ttataagtc tgtgaattt 
      ataggttta cgttttgtg gatattgta gataacaaa 
      tttgtttac tgctggtat tatccatac cgtctgtct 
      gtacacaaa ttctacgct tgtatattt ataacaata 
      aattgttct tttcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY