General information | |||
TC002792 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10234328..10241470 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002792 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00446 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)- Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC002792 RA
MAVKQKVVE CVAQSAEAR LQLILRTLQ DLTAQGLQD KKCANIVSL LGTIHVPTD PSDYKQFIG SIKSVQEIL TKECMKNDL VYLTLRSLY HEISTSQKP LSPAMSIVL TLIQVDYIP TAVDWILQT GYSPENHKK ALMTLCNWL PKWTWTENL GRLVLTFMQ GLEAQQQLE IVFEVTEAT IEPLFRLLI LPEHRKSVG PIVKYMLFS MLSQSNPVS FDKVVPHVG SVVAYLYKE KSDSSLEYF GDIVKVCFL LMDHFSDYS NRYVQLKKD LEQHLPAAN CIENGPSNS VATVYSASG KVGLNNLGN TCYMNSVLQ ALFMTKPFR NEILLYNKD MTSLLSKLQ TLFALLQHS KRASISPHD ISNLARPPG FLPGHQHDS SEFLGYLLD VLHEQEKTA VCGNGTKAD HVPPSYNTI VQSAFGGRT VSVSRCGEC DTKSERVDN FWELQLSFP NTTDNQSVQ TLLNYYLQP EKLSGDNQY HCEVCDCLT DGERVTCIE EAPPRLILT LKHFRYDQA SQQRTKLLQ SIKLDNHVQ LDTGLYVLY AAVVHCGSS VDSGHYYTF ARDGDEWFK FNDCSVMRT MAEELCSLK PPETPYILF YSRQDCSDP EALPRTVLS QRLQLVINN DHTEFELEK RRRPIKTYN SKQNRNDEP PPPGCGGPG FSSNSSNMY VC
- >TC002792 RA
atggcagtg aagcaaaag gtggtggaa tgcgtggcc cagagcgcg gaggcgcgc ctccagctc atcctgcgc accctgcag gacctcacg gcccagggg ctccaggac aagaagtgc gccaacatc gtcagcctc ctggggacc atccacgtg cccacggac cccagcgac tacaagcag ttcatcggg agcattaag agcgtccag gagatcctc accaaagaa tgcatgaag aacgacttg gtctacctg accttgagg tcgctttac cacgaaatc tccacgtct cagaagccg ctctcgccg gccatgtcc atagttttg acccttatc caagtcgat tatatcccg acggccgtg gactggatc ctccaaacg gggtattcc cccgaaaac cacaaaaaa gccctcatg acgctgtgt aattggctg ccgaaatgg acctggacg gagaacctc ggccggttg gtcctcacc ttcatgcag ggcctggag gcccagcaa cagctcgag atcgtgttc gaggtcacg gaggccacg attgagccc cttttcagg ctgttgatt ttgcccgag caccgcaaa agcgtgggc cccattgtc aagtacatg ctgttctcc atgttgtcg caaagcaac cccgtgtcc ttcgacaag gtcgtcccc cacgtgggg agcgtcgtc gcctacttg tacaaagaa aagtccgat tctagtctt gaatatttc ggcgacatc gtcaaagtg tgttttctg ttaatggac cacttctcc gactattca aatcgctac gtccagttg aagaaggac ctggagcag catcttcct gcggcgaat tgtatcgag aacgggccg tcgaattcc gtggcgacc gtttattca gcgtcgggg aaggtgggg ttgaacaac ctggggaac acctgttac atgaacagt gtcctccag gcgttgttt atgacgaag ccgttccgc aatgagatt cttttgtat aataaggat atgacgtcg ttgttgtcc aagttgcag acgctgttt gcgctgttg caacactcg aagagggct tccatctcg ccgcatgat atttcgaat ttggcgagg ccgcccggg tttttgcca gggcatcag catgacagt tcggagttt ttggggtat ttgttggat gttttgcac gagcaggag aagacggcc gtttgtggg aatggtacc aaagcagac catgtgccc ccctcatac aacaccata gtccagtca gccttcggc ggtcgcacg gtgtcggtt tcgcgttgc ggcgaatgc gacacaaaa agcgaacgc gtcgacaac ttctgggag ctgcaactc tccttcccc aacaccacc gacaatcag tccgtgcag accctcctc aactactac ctccaaccg gagaagctc agcggcgac aaccaatac cactgcgag gtgtgcgac tgtctcacc gacggcgag cgcgtcacc tgcatcgaa gaggctccc ccacgtctc atcctcacc ctgaagcac ttccgctac gaccaggcc tcccagcag cgcaccaaa ctcctccag agcatcaaa ctcgacaat cacgtgcag ctcgacacc ggcctctac gtgctctac gcggcggtg gtccactgc ggctccagt gtggactcg gggcactat tacacgttc gcacgagac ggagacgag tggttcaag ttcaacgat tgttccgtt atgaggact atggcggag gaactttgc agtttgaag ccgccagaa acgccgtat attttgttt tatagtcgg caggactgc tcggatccc gaggcgctg ccgaggact gtcttgtcg cagagatta cagttagtg attaataat gatcacact gagttcgag cttgagaag agaaggagg ccgatcaag acgtacaac agcaaacaa aatcggaac gatgaaccg ccgcctccg gggtgtgga gggcctggg ttttctagt aattcatct aatatgtat gtatgctaa
- >TC002792 RA
ggccattta attatgatt cccgttttt gccgtttaa ttaatttct ttttgtgtt ttatttatt ttgctatca tcacccttc gaaaaatgc gtttgtaag tcttaacga acgcccaaa gtgtgccta aaccaagtt cctccaccg cagctacga ttccaacga ccgtcgaaa aaccctcac ctggatgac attcattgc tatcatctc ccagtaaga aacatggca gtgaagcaa aaggtggtg gaatgcgtg gcccagagc gcggaggcg cgcctccag ctcatcctg cgcaccctg caggacctc acggcccag gggctccag gacaagaag tgcgccaac atcgtcagc ctcctgggg accatccac gtgcccacg gaccccagc gactacaag cagttcatc gggagcatt aagagcgtc caggagatc ctcaccaaa gaatgcatg aagaacgac ttggtctac ctgaccttg aggtcgctt taccacgaa atctccacg tctcagaag ccgctctcg ccggccatg tccatagtt ttgaccctt atccaagtc gattatatc ccgacggcc gtggactgg atcctccaa acggggtat tcccccgaa aaccacaaa aaagccctc atgacgctg tgtaattgg ctgccgaaa tggacctgg acggagaac ctcggccgg ttggtcctc accttcatg cagggcctg gaggcccag caacagctc gagatcgtg ttcgaggtc acggaggcc acgattgag ccccttttc aggctgttg attttgccc gagcaccgc aaaagcgtg ggccccatt gtcaagtac atgctgttc tccatgttg tcgcaaagc aaccccgtg tccttcgac aaggtcgtc ccccacgtg gggagcgtc gtcgcctac ttgtacaaa gaaaagtcc gattctagt cttgaatat ttcggcgac atcgtcaaa gtgtgtttt ctgttaatg gaccacttc tccgactat tcaaatcgc tacgtccag ttgaagaag gacctggag cagcatctt cctgcggcg aattgtatc gagaacggg ccgtcgaat tccgtggcg accgtttat tcagcgtcg gggaaggtg gggttgaac aacctgggg aacacctgt tacatgaac agtgtcctc caggcgttg tttatgacg aagccgttc cgcaatgag attcttttg tataataag gatatgacg tcgttgttg tccaagttg cagacgctg tttgcgctg ttgcaacac tcgaagagg gcttccatc tcgccgcat gatatttcg aatttggcg aggccgccc gggtttttg ccagggcat cagcatgac agttcggag tttttgggg tatttgttg gatgttttg cacgagcag gagaagacg gccgtttgt gggaatggt accaaagca gaccatgtg cccccctca tacaacacc atagtccag tcagccttc ggcggtcgc acggtgtcg gtttcgcgt tgcggcgaa tgcgacaca aaaagcgaa cgcgtcgac aacttctgg gagctgcaa ctctccttc cccaacacc accgacaat cagtccgtg cagaccctc ctcaactac tacctccaa ccggagaag ctcagcggc gacaaccaa taccactgc gaggtgtgc gactgtctc accgacggc gagcgcgtc acctgcatc gaagaggct cccccacgt ctcatcctc accctgaag cacttccgc tacgaccag gcctcccag cagcgcacc aaactcctc cagagcatc aaactcgac aatcacgtg cagctcgac accggcctc tacgtgctc tacgcggcg gtggtccac tgcggctcc agtgtggac tcggggcac tattacacg ttcgcacga gacggagac gagtggttc aagttcaac gattgttcc gttatgagg actatggcg gaggaactt tgcagtttg aagccgcca gaaacgccg tatattttg ttttatagt cggcaggac tgctcggat cccgaggcg ctgccgagg actgtcttg tcgcagaga ttacagtta gtgattaat aatgatcac actgagttc gagcttgag aagagaagg aggccgatc aagacgtac aacagcaaa caaaatcgg aacgatgaa ccgccgcct ccggggtgt ggagggcct gggttttct agtaattca tctaatatg tatgtatgc taaatgttt caatatata tcggatttg ggttattac atagttgaa ttctttcta atatgttga acaacgaac tgaactcat ctgttggca atttggcga tagggtcag gtggggtaa gtgttaact aggggtctc aattaatat tcggaatac agttgggat gattgattt catagtgtg ccccacccg accctggtt attttgccg gtggataac ttttcgccc agtgacata tacgattgt aaatattag ttttattca cactcacac ataaatagg aattgtttc aaaaatagt cacggagta ttgttgttt tattgttgt ttttgtttt attttgaaa gtacaacgt actgaattg ttttttttt cgaaatcgt gattaattt gctttccat caattgtct aggtttgtt tacacttat ttataagtc tgtgaattt ataggttta cgttttgtg gatattgta gataacaaa tttgtttac tgctggtat tatccatac cgtctgtct gtacacaaa ttctacgct tgtatattt ataacaata aattgttct tttcata
