| General information | |||
| TC002791 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10275284..10288381 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002791 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00445 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC002791 RA
MKRKRRDEC SSPLLDESP KRTKVHAQR KFAQGSNVN SPVITPVKE IEKQEVDVL PEPVELLPI KRPNTEDFL TFLCFRGTP ILPPSLNFF NTASIVDTN GQVHEIKSE SPKNGPIDT STIAKAGSG SSDRPFIAF GVRKRADPI VISRQMDKK RRHALAVQA LRRKYQEQR MAKIRAVTI SKLSEKVAS KTLVRSNSV SKTEAVKKN ATVQKSKVS VEPKQVKVA TRSVKTALR PQIKQKMCL RSFRGRFVQ KELQFRNVK KRPLPPVKK PVKKSEETS EYSSDDDKP LKTSKSVVP KVAKKSAPG PKKPIEINF RITRSNKFL NNKIVSRRQ QIKQRMLLS TPKTTVKPR TLRSQTAQK SKIETAKKV LIKSKEVGK KETVVKKLI ETKTKELKV EALKKSDRL KKDVSPKME KPPPNVKKD VDSGKKTKI VPKPTPKKD ETSSEKKDL VKKKAVMKK TAQKPAESP PRKDPPSKK VDEDKKIDK PTEQMESRA SQRPTRKTK EAAAIYMEI LSHKLVNES RLDDDNVSI DSFPELPNV KKTEQRENE LKAKAKTTK TSESESDTK KSPKKEMEV ESKVLRSGS DKKKSEEKV KKSEDKVKK SEEPVCDRK TRSAVASPL ELKVHINAI EVTSEKKKE KKGKEKFED DESSNSDVS DNNLKTKDK SGKSDEGTK RQTRNSSQN IRHLEDTDD SDESFRADI RIPRKKQIT RSKVSKNCD SENDKNSLK KSLRTNSRS YRECSSSES DQSTTSDIK PTIVNKTKK FTKTRSVQK GEQSFSDSD EEPLSKLTQ NKLSDGKDV AIKEEPKES GEKETKPKR ECAKRPQNY LPMFSSSDE DENYFQGFK TKPEKPKIT TKLPSPGCS HANTTVDLK DISRRFGKE KVNMSTEQI EKWLKDSAM AGSSVKNEN DAMLKFGEK IPTETSHSV ANSIDTEKL KSLLITNIE KKEVSPKGL PNEIKAILA KPMITDRKL IFRNKDRVG TVVGVSPNV NAFSVSNES SVYAFGEEE DVMKTPFRR PTRRPSSTA TSRSEDESS KFEEFAKFR KPTLVKQES EDNLPLGGT EDSPFFTPQ RVTKPKIDK KPMVPPKPP LKQTPKSCN SDASEEFKY KVPSSPSAS SSSSAKLYK RQCTKPKIR ASEVVPVKL PDFPVNGAQ LVEGPTFHP SEKEFQDPL EYIEKIRPK AEQFGICRI VPPSTFKPE CKVTDDMRF TAYNQYVHK MLHRWGPNF KELMAIKKY LSTQNINLT HPPWIGGME IDLPRLYQT VQSLGGLKE VIEKKKWPK VSDLMKIPK SAQDRVTKL DDIYCKYLL PYDTLSPAE REKLFDEVE TEWAKRESK SLLKAQQNI DDNNTEIES ESDDEAEEC IVKGRSMAL NAFYRIARN TMSMWFRTC EPPAEEVEQ EFWKHVTVK QNHICVHSG SIDSGNWGY GFAVSKNSP FARHAWNLK VLTNNSGSV LRSMGPVMG VTVPTLHVG MVFSSCCWY RDPHSLPWI EYLHTGGNK IWYGIPDST SGAFHTALK TLVPNYCRN KSLWLPSDT VMVPPNLLV ENGVSLSRI VQEPGQFII VFPKAFTSS LSTGYVVSE SVYFAPSYW LKTGRLVFN ELRNSCEPS MFSFDRLLL SIVNDARSN VEVLKQIIP AVQELCDKE KSARERVRK LGVSATEKL PLPDAPKKK KKLHNEDGE YECEICRMN LFVSMVIES QEDLVYCLD HAAEQIEQK KIQCKNCTL SFTYDDTEL DGLVDKIKS AICNKLQKK VPNRFTGMP TLLK
- >TC002791 RA
atgaaacgc aaacgccgc gacgagtgt tcatccccc ttactcgac gaaagcccc aaaaggacc aaagtgcac gcgcagcgc aaattcgcc caaggctcc aacgtaaac agccccgtt ataacccca gtcaaagaa atcgaaaaa caagaagtg gacgtcctt cccgaaccc gtggagctg ctccccatc aagagacca aacaccgaa gacttcttg acgtttctg tgcttcagg ggaacccca atcctcccc ccgagcttg aatttcttc aacacggct tcgatagtg gacaccaat ggccaagtg cacgaaatc aagagtgaa tccccgaaa aacggcccc attgacact tccacgatc gctaaagcg ggctcgggg tcctccgac cggccgttc attgcgttt ggggtccga aaacgcgcg gaccccatt gttatcagc cgccaaatg gacaaaaaa cggcggcat gctctcgca gtccaagcg ttgagaagg aagtaccag gagcaaaga atggcgaaa attcgggct gttaccata agcaaactg tcggaaaaa gtcgccagt aaaacgcta gttcgctcg aattccgtg tcgaaaacc gaagcggtt aagaaaaac gccacggtt cagaaaagt aaagttagc gttgaaccg aaacaagtg aaagtggcg acgcgttcg gttaaaacc gccctcagg ccgcagatc aagcaaaaa atgtgtttg cgcagtttc aggggacgg tttgtgcag aaagagttg cagtttaga aatgtgaag aaaaggccg ttgccaccg gttaaaaaa ccggttaag aagagtgaa gaaacgagt gaatattcg tcagatgat gataaaccg ttgaagacg tcgaaaagt gtggtccct aaagtggcg aagaagtcg gcgccgggg cccaaaaaa ccaatagaa atcaatttc agaattacc cgttcgaat aaatttctg aacaacaaa atcgtttca aggcgacaa cagatcaaa caacggatg ttgttatcc acaccgaaa acaacagtc aagcctaga actttaaga tcgcaaact gcgcaaaaa tcaaagata gaaacggcc aaaaaagtt ttaataaaa agcaaagaa gttggtaaa aaagaaaca gttgtaaag aaattaata gaaacaaaa acgaaagaa ttaaaagtt gaagctctt aaaaaatca gatcgttta aagaaagat gtttctcca aaaatggaa aaaccacca ccaaacgtc aaaaaagac gtagacagt gggaagaaa acgaaaatt gttccgaaa ccaacgcca aaaaaagac gaaacatcg agtgagaag aaagatttg gttaagaag aaagctgta atgaagaaa acggctcaa aaaccggcc gaaagtcca ccacgcaaa gacccaccc agtaaaaaa gtggatgaa gataagaaa attgataaa ccaaccgaa caaatggaa tcgagagct tctcaaaga ccgactcgc aaaaccaaa gaagctgcc gccatctat atggaaatt cttagtcac aaattagta aacgagagt cgactcgat gacgataat gtctcaatt gacagtttc ccagaattg ccaaatgtg aaaaaaacc gaacagagg gaaaacgaa ttaaaagcg aaagcaaaa acgacgaaa acatcggag agtgaaagt gataccaaa aaatcaccg aaaaaggaa atggaggtg gaaagtaaa gttttgagg tctggtagt gataagaag aaaagtgaa gaaaaggtg aagaaaagt gaagataag gttaagaaa agtgaagaa ccggtttgt gatcgtaaa acccgatcg gcggtcgct tcacccttg gagcttaag gtgcacata aatgcgatc gaggtcact tccgagaag aagaaagag aagaaaggc aaagaaaaa ttcgaagat gatgaaagt tcgaactcg gacgtctct gacaataat ttgaaaacg aaagataaa agtggaaaa agtgatgaa gggactaaa cgacaaacc aggaactcg tcccaaaac atcaggcat ttggaggat actgatgat tccgacgag tcctttcgt gcggacatc aggattccg aggaaaaaa caaataacc agaagtaaa gtctcaaaa aactgtgac agtgagaac gacaaaaac tctctcaaa aaatcgcta agaacgaat tcgaggagt tacagagag tgcagttcg agtgaatct gaccagtcc accacctcc gatataaaa ccgaccata gttaataaa accaaaaaa tttacaaaa acgagaagt gtgcaaaaa ggggaacaa agcttcagc gattcggac gaagaacca ctttcgaaa ctaacacaa aataaactt agtgacggt aaagatgtt gcaattaag gaagaaccg aaagaaagt ggggaaaaa gaaaccaaa ccgaagcgc gaatgcgcc aaacgcccc caaaactat ctcccgatg ttctcatca tcggacgaa gacgaaaac tactttcaa ggtttcaaa acaaaacct gaaaaaccc aaaattacg actaaactg ccatcgcct ggctgctct cacgccaac accactgtg gaccttaaa gacataagc cgacgtttc ggcaaagaa aaggttaac atgtcgacc gaacagatc gaaaagtgg ctcaaagac tccgccatg gctggaagt agcgtcaaa aatgaaaat gacgctatg ttgaagttt ggtgaaaaa ataccaact gagacctcg cacagtgtt gccaattca attgacacg gagaaacta aaatcgttg cttattaca aacatcgaa aagaaggaa gtgtcaccg aaaggtctt ccgaacgaa attaaggca attttggcc aaaccaatg atcaccgat cggaaatta attttccgc aataaagat cgagtcggg acagttgta ggggtttcc cccaatgtc aacgccttc tccgttagt aacgagagc agtgtttat gcgttcggt gaagaggag gacgtaatg aagactcct ttcaggagg ccaactaga cgtccatca agcactgcc acttcaaga tcggaagat gaatcctca aaatttgag gagtttgca aaattccgc aaaccaacc cttgtcaag caagaatct gaagataac ttacccctt ggtggcacc gaagatagt cccttcttt accccacag agggtaact aagccaaaa attgacaaa aaacccatg gtgccacca aaacctccc cttaaacaa acccccaaa tcgtgcaat tctgacgct tcggaagag tttaaatac aaagtacca agttcgccg agcgctagc tcctcatcc agtgcgaaa ttgtacaaa cgacaatgc actaaacct aaaatccgg gcgagtgaa gtggttccg gtcaagtta ccagatttt ccagtcaac ggggcacag ttagtggaa ggaccgact tttcatcct agtgagaag gagtttcaa gatccgttg gaatatatc gagaagatt cggcccaaa gctgaacag ttcgggatt tgtcggatt gtaccaccg agtactttt aaaccggag tgtaaagtg accgatgat atgaggttt actgcgtat aatcagtac gttcataaa atgttgcat cggtggggg cccaacttt aaggagttg atggcgatt aagaagtat ctgtccacg cagaatatt aatctgact catccacca tggattggc ggtatggag attgacttg ccacgtctc taccaaacc gtccagtcc ctcggcggt ctcaaagaa gtcatcgag aagaagaaa tggccgaaa gtttccgac ctcatgaaa ataccaaag tcggcacaa gacagagtc acgaaacta gacgacatc tattgcaaa tatctatta ccatacgat actttatca cctgccgaa cgcgaaaaa ttatttgac gaagtcgaa accgaatgg gcaaaacgc gagtcaaaa tccctcctc aaagcccaa caaaacatc gacgataac aacactgag atcgagtcg gaatccgac gatgaagcc gaagagtgt attgtcaaa gggcgcagt atggcactg aatgcgttt taccgaatt gcaagaaat acaatgagt atgtggttc aggacttgt gaaccgccg gccgaagag gtcgagcaa gagttctgg aaacatgtg actgtgaaa caaaaccat atttgtgtc cattcgggc tcgattgac tccgggaat tggggctat ggatttgca gtctcgaaa aatagcccc tttgccaga cacgcgtgg aatctcaaa gtgctgacg aataatagt gggtcggta ctaagatcc atgggtcca gttatgggc gtaacggta ccaaccctg cacgtggga atggtgttc agttcgtgc tgttggtac cgcgacccc cacagcctc ccatggatc gagtacctc cacactggg ggtaacaaa atctggtac ggcattccc gactcaacc agtggtgct tttcacacc gcgttgaaa accttagta cctaattac tgtcgaaat aagagtctc tggttacca tcagacaca gtgatggta ccaccaaac ctcctagtc gaaaatgga gtctcactg tctcgaatt gtgcaagaa ccgggacag tttattata gtttttcct aaagcgttc acctcgagt ttaagtacc ggttacgtg gtgtccgaa agtgtgtat ttcgcgcct tcctattgg ctgaagacc ggcagactt gtttttaac gagctgaga aacagttgc gaaccgtcg atgttttcg ttcgataga cttttgtta agtatagtg aacgacgcg aggtcgaat gtggaggtt ttgaagcag atcatacca gcggttcag gagctatgt gataaggaa aagtcggcg agagaaagg gttagaaag ctgggcgtt tcagcgacg gaaaaattg cctctgccg gacgcccca aaaaagaaa aagaagctt cataatgaa gacggggag tacgagtgt gaaatttgc agaatgaat ctttttgtt tcaatggtg atcgaatcg caggaagac ctggtttat tgtctagac catgcggct gaacaaatc gaacaaaag aaaatacaa tgcaagaac tgtactttg agttttacg tacgatgat acggagttg gatggactg gttgataaa atcaagtcg gcgatttgt aacaaattg caaaagaaa gtacctaac cgatttaca gggatgcca acgttgctt aaatga
- >TC002791 RA
aataaaata aaataataa aaatgaaaa aaacttacc ttgtactcc aagaaccta cctcgcatt ccatgtgaa gcttgagcc cccgcaaac catgcaaaa acaccactt ttcagtcaa ttttggcac aacacacgc acaagacaa ttttttcac ttttctaac aataaaaca ccaagccga cttgtcact tgatttttg aaataaccc aaacgcgca tgcgtaaga cgcgaataa aaaagtgtc actatctat taatttaaa gaatttatt aaaatacgt ttactattt tgcgttttg cgtatttcg attattgtc cagctttca aaataaacg ttttgcagt ctctttttt ggcccttac gtttcactt ggcggtagt tttcaagta aaaattcgg gcaaccaac agaaaagac caccgaaag cgttggtaa catcagtgc ggccaacac attgcggcc ataaacaaa aataaaaaa ctctttgtg ttaatttta aacagtatt aagcaattt taaggtaat tacaatgtg ataaacact tcactgtgt gttgtagag tatttctat atagtttat gcgagattt taatgaaga aattgaata aaaaagtta ggttatgta agtgagaat gtgttggta gaaatcgtg ggttttttc aaaaaaatg gtaataaac cggggcgaa atgaaacgc aaacgccgc gacgagtgt tcatccccc ttactcgac gaaagcccc aaaaggacc aaagtgcac gcgcagcgc aaattcgcc caaggctcc aacgtaaac agccccgtt ataacccca gtcaaagaa atcgaaaaa caagaagtg gacgtcctt cccgaaccc gtggagctg ctccccatc aagagacca aacaccgaa gacttcttg acgtttctg tgcttcagg ggaacccca atcctcccc ccgagcttg aatttcttc aacacggct tcgatagtg gacaccaat ggccaagtg cacgaaatc aagagtgaa tccccgaaa aacggcccc attgacact tccacgatc gctaaagcg ggctcgggg tcctccgac cggccgttc attgcgttt ggggtccga aaacgcgcg gaccccatt gttatcagc cgccaaatg gacaaaaaa cggcggcat gctctcgca gtccaagcg ttgagaagg aagtaccag gagcaaaga atggcgaaa attcgggct gttaccata agcaaactg tcggaaaaa gtcgccagt aaaacgcta gttcgctcg aattccgtg tcgaaaacc gaagcggtt aagaaaaac gccacggtt cagaaaagt aaagttagc gttgaaccg aaacaagtg aaagtggcg acgcgttcg gttaaaacc gccctcagg ccgcagatc aagcaaaaa atgtgtttg cgcagtttc aggggacgg tttgtgcag aaagagttg cagtttaga aatgtgaag aaaaggccg ttgccaccg gttaaaaaa ccggttaag aagagtgaa gaaacgagt gaatattcg tcagatgat gataaaccg ttgaagacg tcgaaaagt gtggtccct aaagtggcg aagaagtcg gcgccgggg cccaaaaaa ccaatagaa atcaatttc agaattacc cgttcgaat aaatttctg aacaacaaa atcgtttca aggcgacaa cagatcaaa caacggatg ttgttatcc acaccgaaa acaacagtc aagcctaga actttaaga tcgcaaact gcgcaaaaa tcaaagata gaaacggcc aaaaaagtt ttaataaaa agcaaagaa gttggtaaa aaagaaaca gttgtaaag aaattaata gaaacaaaa acgaaagaa ttaaaagtt gaagctctt aaaaaatca gatcgttta aagaaagat gtttctcca aaaatggaa aaaccacca ccaaacgtc aaaaaagac gtagacagt gggaagaaa acgaaaatt gttccgaaa ccaacgcca aaaaaagac gaaacatcg agtgagaag aaagatttg gttaagaag aaagctgta atgaagaaa acggctcaa aaaccggcc gaaagtcca ccacgcaaa gacccaccc agtaaaaaa gtggatgaa gataagaaa attgataaa ccaaccgaa caaatggaa tcgagagct tctcaaaga ccgactcgc aaaaccaaa gaagctgcc gccatctat atggaaatt cttagtcac aaattagta aacgagagt cgactcgat gacgataat gtctcaatt gacagtttc ccagaattg ccaaatgtg aaaaaaacc gaacagagg gaaaacgaa ttaaaagcg aaagcaaaa acgacgaaa acatcggag agtgaaagt gataccaaa aaatcaccg aaaaaggaa atggaggtg gaaagtaaa gttttgagg tctggtagt gataagaag aaaagtgaa gaaaaggtg aagaaaagt gaagataag gttaagaaa agtgaagaa ccggtttgt gatcgtaaa acccgatcg gcggtcgct tcacccttg gagcttaag gtgcacata aatgcgatc gaggtcact tccgagaag aagaaagag aagaaaggc aaagaaaaa ttcgaagat gatgaaagt tcgaactcg gacgtctct gacaataat ttgaaaacg aaagataaa agtggaaaa agtgatgaa gggactaaa cgacaaacc aggaactcg tcccaaaac atcaggcat ttggaggat actgatgat tccgacgag tcctttcgt gcggacatc aggattccg aggaaaaaa caaataacc agaagtaaa gtctcaaaa aactgtgac agtgagaac gacaaaaac tctctcaaa aaatcgcta agaacgaat tcgaggagt tacagagag tgcagttcg agtgaatct gaccagtcc accacctcc gatataaaa ccgaccata gttaataaa accaaaaaa tttacaaaa acgagaagt gtgcaaaaa ggggaacaa agcttcagc gattcggac gaagaacca ctttcgaaa ctaacacaa aataaactt agtgacggt aaagatgtt gcaattaag gaagaaccg aaagaaagt ggggaaaaa gaaaccaaa ccgaagcgc gaatgcgcc aaacgcccc caaaactat ctcccgatg ttctcatca tcggacgaa gacgaaaac tactttcaa ggtttcaaa acaaaacct gaaaaaccc aaaattacg actaaactg ccatcgcct ggctgctct cacgccaac accactgtg gaccttaaa gacataagc cgacgtttc ggcaaagaa aaggttaac atgtcgacc gaacagatc gaaaagtgg ctcaaagac tccgccatg gctggaagt agcgtcaaa aatgaaaat gacgctatg ttgaagttt ggtgaaaaa ataccaact gagacctcg cacagtgtt gccaattca attgacacg gagaaacta aaatcgttg cttattaca aacatcgaa aagaaggaa gtgtcaccg aaaggtctt ccgaacgaa attaaggca attttggcc aaaccaatg atcaccgat cggaaatta attttccgc aataaagat cgagtcggg acagttgta ggggtttcc cccaatgtc aacgccttc tccgttagt aacgagagc agtgtttat gcgttcggt gaagaggag gacgtaatg aagactcct ttcaggagg ccaactaga cgtccatca agcactgcc acttcaaga tcggaagat gaatcctca aaatttgag gagtttgca aaattccgc aaaccaacc cttgtcaag caagaatct gaagataac ttacccctt ggtggcacc gaagatagt cccttcttt accccacag agggtaact aagccaaaa attgacaaa aaacccatg gtgccacca aaacctccc cttaaacaa acccccaaa tcgtgcaat tctgacgct tcggaagag tttaaatac aaagtacca agttcgccg agcgctagc tcctcatcc agtgcgaaa ttgtacaaa cgacaatgc actaaacct aaaatccgg gcgagtgaa gtggttccg gtcaagtta ccagatttt ccagtcaac ggggcacag ttagtggaa ggaccgact tttcatcct agtgagaag gagtttcaa gatccgttg gaatatatc gagaagatt cggcccaaa gctgaacag ttcgggatt tgtcggatt gtaccaccg agtactttt aaaccggag tgtaaagtg accgatgat atgaggttt actgcgtat aatcagtac gttcataaa atgttgcat cggtggggg cccaacttt aaggagttg atggcgatt aagaagtat ctgtccacg cagaatatt aatctgact catccacca tggattggc ggtatggag attgacttg ccacgtctc taccaaacc gtccagtcc ctcggcggt ctcaaagaa gtcatcgag aagaagaaa tggccgaaa gtttccgac ctcatgaaa ataccaaag tcggcacaa gacagagtc acgaaacta gacgacatc tattgcaaa tatctatta ccatacgat actttatca cctgccgaa cgcgaaaaa ttatttgac gaagtcgaa accgaatgg gcaaaacgc gagtcaaaa tccctcctc aaagcccaa caaaacatc gacgataac aacactgag atcgagtcg gaatccgac gatgaagcc gaagagtgt attgtcaaa gggcgcagt atggcactg aatgcgttt taccgaatt gcaagaaat acaatgagt atgtggttc aggacttgt gaaccgccg gccgaagag gtcgagcaa gagttctgg aaacatgtg actgtgaaa caaaaccat atttgtgtc cattcgggc tcgattgac tccgggaat tggggctat ggatttgca gtctcgaaa aatagcccc tttgccaga cacgcgtgg aatctcaaa gtgctgacg aataatagt gggtcggta ctaagatcc atgggtcca gttatgggc gtaacggta ccaaccctg cacgtggga atggtgttc agttcgtgc tgttggtac cgcgacccc cacagcctc ccatggatc gagtacctc cacactggg ggtaacaaa atctggtac ggcattccc gactcaacc agtggtgct tttcacacc gcgttgaaa accttagta cctaattac tgtcgaaat aagagtctc tggttacca tcagacaca gtgatggta ccaccaaac ctcctagtc gaaaatgga gtctcactg tctcgaatt gtgcaagaa ccgggacag tttattata gtttttcct aaagcgttc acctcgagt ttaagtacc ggttacgtg gtgtccgaa agtgtgtat ttcgcgcct tcctattgg ctgaagacc ggcagactt gtttttaac gagctgaga aacagttgc gaaccgtcg atgttttcg ttcgataga cttttgtta agtatagtg aacgacgcg aggtcgaat gtggaggtt ttgaagcag atcatacca gcggttcag gagctatgt gataaggaa aagtcggcg agagaaagg gttagaaag ctgggcgtt tcagcgacg gaaaaattg cctctgccg gacgcccca aaaaagaaa aagaagctt cataatgaa gacggggag tacgagtgt gaaatttgc agaatgaat ctttttgtt tcaatggtg atcgaatcg caggaagac ctggtttat tgtctagac catgcggct gaacaaatc gaacaaaag aaaatacaa tgcaagaac tgtactttg agttttacg tacgatgat acggagttg gatggactg gttgataaa atcaagtcg gcgatttgt aacaaattg caaaagaaa gtacctaac cgatttaca gggatgcca acgttgctt aaatgaatt cagcgtgca aatatttta ttgtagatt gatgtattt ttttaggtt ggtattgtg tggattttt ctttacata aacgatttt agtcataac aggtgtatt tttatttat acataaatt ataagtatt taattcact tagtggtaa ctagatgtg aacgaactg atataggat gcgagtaag gttacgaga ttttttgtg tctcttgtg gtgagaaat ttaaactac cataaataa agtcaacag tcacgtcat caaaactaa aataaaact tctctcttc accgttcta attattatt aaggggttt tgctgatta ttaagtttt ttcttagtt aaaatgata aaaaaacgg aaataccat cattcaaaa tgaa
