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Gene information
General information
TC002791 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 5713997..5727094
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002791
    iBeetle screen iB_00445
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10

    wings not closed - Number of animals showing the phenotype: 10
    legs orientation irregular - Number of animals showing the phenotype: 10
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002791 RA
      MKRKRRDEC SSPLLDESP KRTKVHAQR KFAQGSNVN 
      SPVITPVKE IEKQEVDVL PEPVELLPI KRPNTEDFL 
      TFLCFRGTP ILPPSLNFF NTASIVDTN GQVHEIKSE 
      SPKNGPIDT STIAKAGSG SSDRPFIAF GVRKRADPI 
      VISRQMDKK RRHALAVQA LRRKYQEQR MAKIRAVTI 
      SKLSEKVAS KTLVRSNSV SKTEAVKKN ATVQKSKVS 
      VEPKQVKVA TRSVKTALR PQIKQKMCL RSFRGRFVQ 
      KELQFRNVK KRPLPPVKK PVKKSEETS EYSSDDDKP 
      LKTSKSVVP KVAKKSAPG PKKPIEINF RITRSNKFL 
      NNKIVSRRQ QIKQRMLLS TPKTTVKPR TLRSQTAQK 
      SKIETAKKV LIKSKEVGK KETVVKKLI ETKTKELKV 
      EALKKSDRL KKDVSPKME KPPPNVKKD VDSGKKTKI 
      VPKPTPKKD ETSSEKKDL VKKKAVMKK TAQKPAESP 
      PRKDPPSKK VDEDKKIDK PTEQMESRA SQRPTRKTK 
      EAAAIYMEI LSHKLVNES RLDDDNVSI DSFPELPNV 
      KKTEQRENE LKAKAKTTK TSESESDTK KSPKKEMEV 
      ESKVLRSGS DKKKSEEKV KKSEDKVKK SEEPVCDRK 
      TRSAVASPL ELKVHINAI EVTSEKKKE KKGKEKFED 
      DESSNSDVS DNNLKTKDK SGKSDEGTK RQTRNSSQN 
      IRHLEDTDD SDESFRADI RIPRKKQIT RSKVSKNCD 
      SENDKNSLK KSLRTNSRS YRECSSSES DQSTTSDIK 
      PTIVNKTKK FTKTRSVQK GEQSFSDSD EEPLSKLTQ 
      NKLSDGKDV AIKEEPKES GEKETKPKR ECAKRPQNY 
      LPMFSSSDE DENYFQGFK TKPEKPKIT TKLPSPGCS 
      HANTTVDLK DISRRFGKE KVNMSTEQI EKWLKDSAM 
      AGSSVKNEN DAMLKFGEK IPTETSHSV ANSIDTEKL 
      KSLLITNIE KKEVSPKGL PNEIKAILA KPMITDRKL 
      IFRNKDRVG TVVGVSPNV NAFSVSNES SVYAFGEEE 
      DVMKTPFRR PTRRPSSTA TSRSEDESS KFEEFAKFR 
      KPTLVKQES EDNLPLGGT EDSPFFTPQ RVTKPKIDK 
      KPMVPPKPP LKQTPKSCN SDASEEFKY KVPSSPSAS 
      SSSSAKLYK RQCTKPKIR ASEVVPVKL PDFPVNGAQ 
      LVEGPTFHP SEKEFQDPL EYIEKIRPK AEQFGICRI 
      VPPSTFKPE CKVTDDMRF TAYNQYVHK MLHRWGPNF 
      KELMAIKKY LSTQNINLT HPPWIGGME IDLPRLYQT 
      VQSLGGLKE VIEKKKWPK VSDLMKIPK SAQDRVTKL 
      DDIYCKYLL PYDTLSPAE REKLFDEVE TEWAKRESK 
      SLLKAQQNI DDNNTEIES ESDDEAEEC IVKGRSMAL 
      NAFYRIARN TMSMWFRTC EPPAEEVEQ EFWKHVTVK 
      QNHICVHSG SIDSGNWGY GFAVSKNSP FARHAWNLK 
      VLTNNSGSV LRSMGPVMG VTVPTLHVG MVFSSCCWY 
      RDPHSLPWI EYLHTGGNK IWYGIPDST SGAFHTALK 
      TLVPNYCRN KSLWLPSDT VMVPPNLLV ENGVSLSRI 
      VQEPGQFII VFPKAFTSS LSTGYVVSE SVYFAPSYW 
      LKTGRLVFN ELRNSCEPS MFSFDRLLL SIVNDARSN 
      VEVLKQIIP AVQELCDKE KSARERVRK LGVSATEKL 
      PLPDAPKKK KKLHNEDGE YECEICRMN LFVSMVIES 
      QEDLVYCLD HAAEQIEQK KIQCKNCTL SFTYDDTEL 
      DGLVDKIKS AICNKLQKK VPNRFTGMP TLLK
    CDS sequence
    • >TC002791 RA
      atgaaacgc aaacgccgc gacgagtgt tcatccccc 
      ttactcgac gaaagcccc aaaaggacc aaagtgcac 
      gcgcagcgc aaattcgcc caaggctcc aacgtaaac 
      agccccgtt ataacccca gtcaaagaa atcgaaaaa 
      caagaagtg gacgtcctt cccgaaccc gtggagctg 
      ctccccatc aagagacca aacaccgaa gacttcttg 
      acgtttctg tgcttcagg ggaacccca atcctcccc 
      ccgagcttg aatttcttc aacacggct tcgatagtg 
      gacaccaat ggccaagtg cacgaaatc aagagtgaa 
      tccccgaaa aacggcccc attgacact tccacgatc 
      gctaaagcg ggctcgggg tcctccgac cggccgttc 
      attgcgttt ggggtccga aaacgcgcg gaccccatt 
      gttatcagc cgccaaatg gacaaaaaa cggcggcat 
      gctctcgca gtccaagcg ttgagaagg aagtaccag 
      gagcaaaga atggcgaaa attcgggct gttaccata 
      agcaaactg tcggaaaaa gtcgccagt aaaacgcta 
      gttcgctcg aattccgtg tcgaaaacc gaagcggtt 
      aagaaaaac gccacggtt cagaaaagt aaagttagc 
      gttgaaccg aaacaagtg aaagtggcg acgcgttcg 
      gttaaaacc gccctcagg ccgcagatc aagcaaaaa 
      atgtgtttg cgcagtttc aggggacgg tttgtgcag 
      aaagagttg cagtttaga aatgtgaag aaaaggccg 
      ttgccaccg gttaaaaaa ccggttaag aagagtgaa 
      gaaacgagt gaatattcg tcagatgat gataaaccg 
      ttgaagacg tcgaaaagt gtggtccct aaagtggcg 
      aagaagtcg gcgccgggg cccaaaaaa ccaatagaa 
      atcaatttc agaattacc cgttcgaat aaatttctg 
      aacaacaaa atcgtttca aggcgacaa cagatcaaa 
      caacggatg ttgttatcc acaccgaaa acaacagtc 
      aagcctaga actttaaga tcgcaaact gcgcaaaaa 
      tcaaagata gaaacggcc aaaaaagtt ttaataaaa 
      agcaaagaa gttggtaaa aaagaaaca gttgtaaag 
      aaattaata gaaacaaaa acgaaagaa ttaaaagtt 
      gaagctctt aaaaaatca gatcgttta aagaaagat 
      gtttctcca aaaatggaa aaaccacca ccaaacgtc 
      aaaaaagac gtagacagt gggaagaaa acgaaaatt 
      gttccgaaa ccaacgcca aaaaaagac gaaacatcg 
      agtgagaag aaagatttg gttaagaag aaagctgta 
      atgaagaaa acggctcaa aaaccggcc gaaagtcca 
      ccacgcaaa gacccaccc agtaaaaaa gtggatgaa 
      gataagaaa attgataaa ccaaccgaa caaatggaa 
      tcgagagct tctcaaaga ccgactcgc aaaaccaaa 
      gaagctgcc gccatctat atggaaatt cttagtcac 
      aaattagta aacgagagt cgactcgat gacgataat 
      gtctcaatt gacagtttc ccagaattg ccaaatgtg 
      aaaaaaacc gaacagagg gaaaacgaa ttaaaagcg 
      aaagcaaaa acgacgaaa acatcggag agtgaaagt 
      gataccaaa aaatcaccg aaaaaggaa atggaggtg 
      gaaagtaaa gttttgagg tctggtagt gataagaag 
      aaaagtgaa gaaaaggtg aagaaaagt gaagataag 
      gttaagaaa agtgaagaa ccggtttgt gatcgtaaa 
      acccgatcg gcggtcgct tcacccttg gagcttaag 
      gtgcacata aatgcgatc gaggtcact tccgagaag 
      aagaaagag aagaaaggc aaagaaaaa ttcgaagat 
      gatgaaagt tcgaactcg gacgtctct gacaataat 
      ttgaaaacg aaagataaa agtggaaaa agtgatgaa 
      gggactaaa cgacaaacc aggaactcg tcccaaaac 
      atcaggcat ttggaggat actgatgat tccgacgag 
      tcctttcgt gcggacatc aggattccg aggaaaaaa 
      caaataacc agaagtaaa gtctcaaaa aactgtgac 
      agtgagaac gacaaaaac tctctcaaa aaatcgcta 
      agaacgaat tcgaggagt tacagagag tgcagttcg 
      agtgaatct gaccagtcc accacctcc gatataaaa 
      ccgaccata gttaataaa accaaaaaa tttacaaaa 
      acgagaagt gtgcaaaaa ggggaacaa agcttcagc 
      gattcggac gaagaacca ctttcgaaa ctaacacaa 
      aataaactt agtgacggt aaagatgtt gcaattaag 
      gaagaaccg aaagaaagt ggggaaaaa gaaaccaaa 
      ccgaagcgc gaatgcgcc aaacgcccc caaaactat 
      ctcccgatg ttctcatca tcggacgaa gacgaaaac 
      tactttcaa ggtttcaaa acaaaacct gaaaaaccc 
      aaaattacg actaaactg ccatcgcct ggctgctct 
      cacgccaac accactgtg gaccttaaa gacataagc 
      cgacgtttc ggcaaagaa aaggttaac atgtcgacc 
      gaacagatc gaaaagtgg ctcaaagac tccgccatg 
      gctggaagt agcgtcaaa aatgaaaat gacgctatg 
      ttgaagttt ggtgaaaaa ataccaact gagacctcg 
      cacagtgtt gccaattca attgacacg gagaaacta 
      aaatcgttg cttattaca aacatcgaa aagaaggaa 
      gtgtcaccg aaaggtctt ccgaacgaa attaaggca 
      attttggcc aaaccaatg atcaccgat cggaaatta 
      attttccgc aataaagat cgagtcggg acagttgta 
      ggggtttcc cccaatgtc aacgccttc tccgttagt 
      aacgagagc agtgtttat gcgttcggt gaagaggag 
      gacgtaatg aagactcct ttcaggagg ccaactaga 
      cgtccatca agcactgcc acttcaaga tcggaagat 
      gaatcctca aaatttgag gagtttgca aaattccgc 
      aaaccaacc cttgtcaag caagaatct gaagataac 
      ttacccctt ggtggcacc gaagatagt cccttcttt 
      accccacag agggtaact aagccaaaa attgacaaa 
      aaacccatg gtgccacca aaacctccc cttaaacaa 
      acccccaaa tcgtgcaat tctgacgct tcggaagag 
      tttaaatac aaagtacca agttcgccg agcgctagc 
      tcctcatcc agtgcgaaa ttgtacaaa cgacaatgc 
      actaaacct aaaatccgg gcgagtgaa gtggttccg 
      gtcaagtta ccagatttt ccagtcaac ggggcacag 
      ttagtggaa ggaccgact tttcatcct agtgagaag 
      gagtttcaa gatccgttg gaatatatc gagaagatt 
      cggcccaaa gctgaacag ttcgggatt tgtcggatt 
      gtaccaccg agtactttt aaaccggag tgtaaagtg 
      accgatgat atgaggttt actgcgtat aatcagtac 
      gttcataaa atgttgcat cggtggggg cccaacttt 
      aaggagttg atggcgatt aagaagtat ctgtccacg 
      cagaatatt aatctgact catccacca tggattggc 
      ggtatggag attgacttg ccacgtctc taccaaacc 
      gtccagtcc ctcggcggt ctcaaagaa gtcatcgag 
      aagaagaaa tggccgaaa gtttccgac ctcatgaaa 
      ataccaaag tcggcacaa gacagagtc acgaaacta 
      gacgacatc tattgcaaa tatctatta ccatacgat 
      actttatca cctgccgaa cgcgaaaaa ttatttgac 
      gaagtcgaa accgaatgg gcaaaacgc gagtcaaaa 
      tccctcctc aaagcccaa caaaacatc gacgataac 
      aacactgag atcgagtcg gaatccgac gatgaagcc 
      gaagagtgt attgtcaaa gggcgcagt atggcactg 
      aatgcgttt taccgaatt gcaagaaat acaatgagt 
      atgtggttc aggacttgt gaaccgccg gccgaagag 
      gtcgagcaa gagttctgg aaacatgtg actgtgaaa 
      caaaaccat atttgtgtc cattcgggc tcgattgac 
      tccgggaat tggggctat ggatttgca gtctcgaaa 
      aatagcccc tttgccaga cacgcgtgg aatctcaaa 
      gtgctgacg aataatagt gggtcggta ctaagatcc 
      atgggtcca gttatgggc gtaacggta ccaaccctg 
      cacgtggga atggtgttc agttcgtgc tgttggtac 
      cgcgacccc cacagcctc ccatggatc gagtacctc 
      cacactggg ggtaacaaa atctggtac ggcattccc 
      gactcaacc agtggtgct tttcacacc gcgttgaaa 
      accttagta cctaattac tgtcgaaat aagagtctc 
      tggttacca tcagacaca gtgatggta ccaccaaac 
      ctcctagtc gaaaatgga gtctcactg tctcgaatt 
      gtgcaagaa ccgggacag tttattata gtttttcct 
      aaagcgttc acctcgagt ttaagtacc ggttacgtg 
      gtgtccgaa agtgtgtat ttcgcgcct tcctattgg 
      ctgaagacc ggcagactt gtttttaac gagctgaga 
      aacagttgc gaaccgtcg atgttttcg ttcgataga 
      cttttgtta agtatagtg aacgacgcg aggtcgaat 
      gtggaggtt ttgaagcag atcatacca gcggttcag 
      gagctatgt gataaggaa aagtcggcg agagaaagg 
      gttagaaag ctgggcgtt tcagcgacg gaaaaattg 
      cctctgccg gacgcccca aaaaagaaa aagaagctt 
      cataatgaa gacggggag tacgagtgt gaaatttgc 
      agaatgaat ctttttgtt tcaatggtg atcgaatcg 
      caggaagac ctggtttat tgtctagac catgcggct 
      gaacaaatc gaacaaaag aaaatacaa tgcaagaac 
      tgtactttg agttttacg tacgatgat acggagttg 
      gatggactg gttgataaa atcaagtcg gcgatttgt 
      aacaaattg caaaagaaa gtacctaac cgatttaca 
      gggatgcca acgttgctt aaatga
    mRNA sequence
    • >TC002791 RA
      aataaaata aaataataa aaatgaaaa aaacttacc 
      ttgtactcc aagaaccta cctcgcatt ccatgtgaa 
      gcttgagcc cccgcaaac catgcaaaa acaccactt 
      ttcagtcaa ttttggcac aacacacgc acaagacaa 
      ttttttcac ttttctaac aataaaaca ccaagccga 
      cttgtcact tgatttttg aaataaccc aaacgcgca 
      tgcgtaaga cgcgaataa aaaagtgtc actatctat 
      taatttaaa gaatttatt aaaatacgt ttactattt 
      tgcgttttg cgtatttcg attattgtc cagctttca 
      aaataaacg ttttgcagt ctctttttt ggcccttac 
      gtttcactt ggcggtagt tttcaagta aaaattcgg 
      gcaaccaac agaaaagac caccgaaag cgttggtaa 
      catcagtgc ggccaacac attgcggcc ataaacaaa 
      aataaaaaa ctctttgtg ttaatttta aacagtatt 
      aagcaattt taaggtaat tacaatgtg ataaacact 
      tcactgtgt gttgtagag tatttctat atagtttat 
      gcgagattt taatgaaga aattgaata aaaaagtta 
      ggttatgta agtgagaat gtgttggta gaaatcgtg 
      ggttttttc aaaaaaatg gtaataaac cggggcgaa 
      atgaaacgc aaacgccgc gacgagtgt tcatccccc 
      ttactcgac gaaagcccc aaaaggacc aaagtgcac 
      gcgcagcgc aaattcgcc caaggctcc aacgtaaac 
      agccccgtt ataacccca gtcaaagaa atcgaaaaa 
      caagaagtg gacgtcctt cccgaaccc gtggagctg 
      ctccccatc aagagacca aacaccgaa gacttcttg 
      acgtttctg tgcttcagg ggaacccca atcctcccc 
      ccgagcttg aatttcttc aacacggct tcgatagtg 
      gacaccaat ggccaagtg cacgaaatc aagagtgaa 
      tccccgaaa aacggcccc attgacact tccacgatc 
      gctaaagcg ggctcgggg tcctccgac cggccgttc 
      attgcgttt ggggtccga aaacgcgcg gaccccatt 
      gttatcagc cgccaaatg gacaaaaaa cggcggcat 
      gctctcgca gtccaagcg ttgagaagg aagtaccag 
      gagcaaaga atggcgaaa attcgggct gttaccata 
      agcaaactg tcggaaaaa gtcgccagt aaaacgcta 
      gttcgctcg aattccgtg tcgaaaacc gaagcggtt 
      aagaaaaac gccacggtt cagaaaagt aaagttagc 
      gttgaaccg aaacaagtg aaagtggcg acgcgttcg 
      gttaaaacc gccctcagg ccgcagatc aagcaaaaa 
      atgtgtttg cgcagtttc aggggacgg tttgtgcag 
      aaagagttg cagtttaga aatgtgaag aaaaggccg 
      ttgccaccg gttaaaaaa ccggttaag aagagtgaa 
      gaaacgagt gaatattcg tcagatgat gataaaccg 
      ttgaagacg tcgaaaagt gtggtccct aaagtggcg 
      aagaagtcg gcgccgggg cccaaaaaa ccaatagaa 
      atcaatttc agaattacc cgttcgaat aaatttctg 
      aacaacaaa atcgtttca aggcgacaa cagatcaaa 
      caacggatg ttgttatcc acaccgaaa acaacagtc 
      aagcctaga actttaaga tcgcaaact gcgcaaaaa 
      tcaaagata gaaacggcc aaaaaagtt ttaataaaa 
      agcaaagaa gttggtaaa aaagaaaca gttgtaaag 
      aaattaata gaaacaaaa acgaaagaa ttaaaagtt 
      gaagctctt aaaaaatca gatcgttta aagaaagat 
      gtttctcca aaaatggaa aaaccacca ccaaacgtc 
      aaaaaagac gtagacagt gggaagaaa acgaaaatt 
      gttccgaaa ccaacgcca aaaaaagac gaaacatcg 
      agtgagaag aaagatttg gttaagaag aaagctgta 
      atgaagaaa acggctcaa aaaccggcc gaaagtcca 
      ccacgcaaa gacccaccc agtaaaaaa gtggatgaa 
      gataagaaa attgataaa ccaaccgaa caaatggaa 
      tcgagagct tctcaaaga ccgactcgc aaaaccaaa 
      gaagctgcc gccatctat atggaaatt cttagtcac 
      aaattagta aacgagagt cgactcgat gacgataat 
      gtctcaatt gacagtttc ccagaattg ccaaatgtg 
      aaaaaaacc gaacagagg gaaaacgaa ttaaaagcg 
      aaagcaaaa acgacgaaa acatcggag agtgaaagt 
      gataccaaa aaatcaccg aaaaaggaa atggaggtg 
      gaaagtaaa gttttgagg tctggtagt gataagaag 
      aaaagtgaa gaaaaggtg aagaaaagt gaagataag 
      gttaagaaa agtgaagaa ccggtttgt gatcgtaaa 
      acccgatcg gcggtcgct tcacccttg gagcttaag 
      gtgcacata aatgcgatc gaggtcact tccgagaag 
      aagaaagag aagaaaggc aaagaaaaa ttcgaagat 
      gatgaaagt tcgaactcg gacgtctct gacaataat 
      ttgaaaacg aaagataaa agtggaaaa agtgatgaa 
      gggactaaa cgacaaacc aggaactcg tcccaaaac 
      atcaggcat ttggaggat actgatgat tccgacgag 
      tcctttcgt gcggacatc aggattccg aggaaaaaa 
      caaataacc agaagtaaa gtctcaaaa aactgtgac 
      agtgagaac gacaaaaac tctctcaaa aaatcgcta 
      agaacgaat tcgaggagt tacagagag tgcagttcg 
      agtgaatct gaccagtcc accacctcc gatataaaa 
      ccgaccata gttaataaa accaaaaaa tttacaaaa 
      acgagaagt gtgcaaaaa ggggaacaa agcttcagc 
      gattcggac gaagaacca ctttcgaaa ctaacacaa 
      aataaactt agtgacggt aaagatgtt gcaattaag 
      gaagaaccg aaagaaagt ggggaaaaa gaaaccaaa 
      ccgaagcgc gaatgcgcc aaacgcccc caaaactat 
      ctcccgatg ttctcatca tcggacgaa gacgaaaac 
      tactttcaa ggtttcaaa acaaaacct gaaaaaccc 
      aaaattacg actaaactg ccatcgcct ggctgctct 
      cacgccaac accactgtg gaccttaaa gacataagc 
      cgacgtttc ggcaaagaa aaggttaac atgtcgacc 
      gaacagatc gaaaagtgg ctcaaagac tccgccatg 
      gctggaagt agcgtcaaa aatgaaaat gacgctatg 
      ttgaagttt ggtgaaaaa ataccaact gagacctcg 
      cacagtgtt gccaattca attgacacg gagaaacta 
      aaatcgttg cttattaca aacatcgaa aagaaggaa 
      gtgtcaccg aaaggtctt ccgaacgaa attaaggca 
      attttggcc aaaccaatg atcaccgat cggaaatta 
      attttccgc aataaagat cgagtcggg acagttgta 
      ggggtttcc cccaatgtc aacgccttc tccgttagt 
      aacgagagc agtgtttat gcgttcggt gaagaggag 
      gacgtaatg aagactcct ttcaggagg ccaactaga 
      cgtccatca agcactgcc acttcaaga tcggaagat 
      gaatcctca aaatttgag gagtttgca aaattccgc 
      aaaccaacc cttgtcaag caagaatct gaagataac 
      ttacccctt ggtggcacc gaagatagt cccttcttt 
      accccacag agggtaact aagccaaaa attgacaaa 
      aaacccatg gtgccacca aaacctccc cttaaacaa 
      acccccaaa tcgtgcaat tctgacgct tcggaagag 
      tttaaatac aaagtacca agttcgccg agcgctagc 
      tcctcatcc agtgcgaaa ttgtacaaa cgacaatgc 
      actaaacct aaaatccgg gcgagtgaa gtggttccg 
      gtcaagtta ccagatttt ccagtcaac ggggcacag 
      ttagtggaa ggaccgact tttcatcct agtgagaag 
      gagtttcaa gatccgttg gaatatatc gagaagatt 
      cggcccaaa gctgaacag ttcgggatt tgtcggatt 
      gtaccaccg agtactttt aaaccggag tgtaaagtg 
      accgatgat atgaggttt actgcgtat aatcagtac 
      gttcataaa atgttgcat cggtggggg cccaacttt 
      aaggagttg atggcgatt aagaagtat ctgtccacg 
      cagaatatt aatctgact catccacca tggattggc 
      ggtatggag attgacttg ccacgtctc taccaaacc 
      gtccagtcc ctcggcggt ctcaaagaa gtcatcgag 
      aagaagaaa tggccgaaa gtttccgac ctcatgaaa 
      ataccaaag tcggcacaa gacagagtc acgaaacta 
      gacgacatc tattgcaaa tatctatta ccatacgat 
      actttatca cctgccgaa cgcgaaaaa ttatttgac 
      gaagtcgaa accgaatgg gcaaaacgc gagtcaaaa 
      tccctcctc aaagcccaa caaaacatc gacgataac 
      aacactgag atcgagtcg gaatccgac gatgaagcc 
      gaagagtgt attgtcaaa gggcgcagt atggcactg 
      aatgcgttt taccgaatt gcaagaaat acaatgagt 
      atgtggttc aggacttgt gaaccgccg gccgaagag 
      gtcgagcaa gagttctgg aaacatgtg actgtgaaa 
      caaaaccat atttgtgtc cattcgggc tcgattgac 
      tccgggaat tggggctat ggatttgca gtctcgaaa 
      aatagcccc tttgccaga cacgcgtgg aatctcaaa 
      gtgctgacg aataatagt gggtcggta ctaagatcc 
      atgggtcca gttatgggc gtaacggta ccaaccctg 
      cacgtggga atggtgttc agttcgtgc tgttggtac 
      cgcgacccc cacagcctc ccatggatc gagtacctc 
      cacactggg ggtaacaaa atctggtac ggcattccc 
      gactcaacc agtggtgct tttcacacc gcgttgaaa 
      accttagta cctaattac tgtcgaaat aagagtctc 
      tggttacca tcagacaca gtgatggta ccaccaaac 
      ctcctagtc gaaaatgga gtctcactg tctcgaatt 
      gtgcaagaa ccgggacag tttattata gtttttcct 
      aaagcgttc acctcgagt ttaagtacc ggttacgtg 
      gtgtccgaa agtgtgtat ttcgcgcct tcctattgg 
      ctgaagacc ggcagactt gtttttaac gagctgaga 
      aacagttgc gaaccgtcg atgttttcg ttcgataga 
      cttttgtta agtatagtg aacgacgcg aggtcgaat 
      gtggaggtt ttgaagcag atcatacca gcggttcag 
      gagctatgt gataaggaa aagtcggcg agagaaagg 
      gttagaaag ctgggcgtt tcagcgacg gaaaaattg 
      cctctgccg gacgcccca aaaaagaaa aagaagctt 
      cataatgaa gacggggag tacgagtgt gaaatttgc 
      agaatgaat ctttttgtt tcaatggtg atcgaatcg 
      caggaagac ctggtttat tgtctagac catgcggct 
      gaacaaatc gaacaaaag aaaatacaa tgcaagaac 
      tgtactttg agttttacg tacgatgat acggagttg 
      gatggactg gttgataaa atcaagtcg gcgatttgt 
      aacaaattg caaaagaaa gtacctaac cgatttaca 
      gggatgcca acgttgctt aaatgaatt cagcgtgca 
      aatatttta ttgtagatt gatgtattt ttttaggtt 
      ggtattgtg tggattttt ctttacata aacgatttt 
      agtcataac aggtgtatt tttatttat acataaatt 
      ataagtatt taattcact tagtggtaa ctagatgtg 
      aacgaactg atataggat gcgagtaag gttacgaga 
      ttttttgtg tctcttgtg gtgagaaat ttaaactac 
      cataaataa agtcaacag tcacgtcat caaaactaa 
      aataaaact tctctcttc accgttcta attattatt 
      aaggggttt tgctgatta ttaagtttt ttcttagtt 
      aaaatgata aaaaaacgg aaataccat cattcaaaa 
      tgaa