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Gene information
General information
TC002790 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10289049..10293451
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002790
    iBeetle screen iB_00444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002790 RA
      MQPEGTLAN PLTSDGEDT VSTSSEDDN ECDGAVVLH 
      EDDRPEVPS FGNISVMNS NDIHFGNKT FYQGPVTIK 
      QFLYANGKT TSDKSDSEV VLDATTLAD SGLENPTFV 
      NDNNTASEK TNAASKPAV QENSSGIVK GFRRLQKFF 
      QENVVILVS LLAVLIVSL AILMVVLLL RHSPNPIED 
      DQITREKLD EQEKNTPVD SPIDPQANL TLPPKLKLV 
      SRLEWLAQP PVQPANPLA VPVPYVIIL HTATENCSS 
      QAQCIFHVR FIQTFHIES RSWWDIGYN FLVGGDGEA 
      YEGRGWKSE GAHTYGYNA KSIGIAFIG TFNSFKPPE 
      RQITACKQL IAKGVELGF IRKDYKLLA HRQLETTQS 
      PGAALYEEM KTWEHWAKT P
    CDS sequence
    • >TC002790 RA
      atgcaaccg gaaggaacg ctcgctaac ccgttaacc 
      agcgatggc gaggatacg gtcagtact tcttcagag 
      gatgacaat gagtgtgat ggtgctgtc gtcttacac 
      gaagacgac agaccggaa gtaccgtcg tttggcaat 
      atctcggtg atgaactct aatgatatc cactttgga 
      aataaaacg ttctatcaa ggaccagtg actattaaa 
      cagttctta tacgcgaat ggtaaaacc actagtgac 
      aagagtgat agtgaagtt gtattagat gcgacgaca 
      cttgcagat agtggactt gaaaatccg acgtttgtg 
      aatgataac aatactgcg tcggagaag accaatgct 
      gcgagtaaa cctgcagtg caggaaaat agttcagga 
      attgttaaa ggatttcga cgattacag aaattcttt 
      caagaaaac gttgtgatt ctagtcagc ctcctagca 
      gttttgatt gtatcatta gcaatttta atggttgtg 
      ttattatta agacatagt cctaatccc atcgaagac 
      gatcaaata actagggaa aaattggat gaacaggag 
      aaaaataca ccagtcgac tcgccaata gatccccag 
      gcaaatctg actcttccc cccaaactt aaacttgtc 
      tcccgtctg gaatggctt gcccaacct ccagtccaa 
      cccgccaac cctctcgct gttcccgtc ccctacgtc 
      ataatcctc cacacggcc acagaaaac tgttcctct 
      caagcccaa tgtattttc cacgtccgt ttcatccaa 
      accttccac atcgaaagc cgaagttgg tgggacatt 
      ggatacaat ttcctggtg ggcggcgac ggagaagct 
      tacgaaggc cggggatgg aaatcagaa ggggcacat 
      acgtacgga tacaacgcc aaaagtatc ggaattgcc 
      ttcattggc acttttaat agttttaaa ccaccggag 
      aggcagata acggcttgc aaacaactg attgcaaaa 
      ggggtggaa ttggggttc ataagaaaa gattataag 
      ttgctagcg cacaggcag ctggaaacg acccaaagt 
      ccgggggcg gcgctctat gaggagatg aagacgtgg 
      gagcattgg gcgaaaacg ccgtaa
    mRNA sequence
    • >TC002790 RA
      ttctgttgt aatgtcact accatcagt actaaccaa 
      ttatggtgt ttatcgcaa cgaatccag agctaaaac 
      aaaataaac aaaaatcgc tggtgatcc tcgacctca 
      aagaatgcg ttgcatttt ttctcgtcg tatttttag 
      ccatgttgg ctattattt tcgcacttt cggcaatcg 
      ggattcact tcagtgtaa ccaaggacg atcctttga 
      tccttcctt atgataaat ggacaatcc ctcaaaaat 
      ttttcaacc gggaacaat ggggtgcta aacctcccc 
      ttaaccaaa cccgtctcc aacacccag tggatctag 
      ttataatcg gacataccg tcaccgatt tttgctgga 
      atttcgaac agtgttcac acatcgttg cagctattc 
      aaaactatc atctcgaat taaaaatgt cagatattg 
      cgtacaatt ttttaatcg gaggtgatg gaaatattt 
      atgaagggc ggggttggg atgttgcaa attctcaaa 
      gaaatcact caattagtg ttagtttta tcggtaatt 
      ttattttgg atgagttaa atttaaata tgattgatg 
      ctttccaac aactgatga ttgatggtg ttaatggag 
      aaaaattga gtaaaaaat ataaaataa tgtgtcata 
      atcaaacca aggcaacgt taagtccag gtaaaaatg 
      tatataaaa ttgtgaaaa cctggcccc atttccgct 
      ctggtgtat tgaaaaact aatctgttt ttaattcaa 
      ggaccaaag tttattatt agaaactgt tatcaaatt 
      atagaaaga tttcagtct cggtttcag aggaagttc 
      ggaattcac tttttaacg caaaaagac ctaaggcac 
      ataagcatc taaaactgg atccaaacc ttgaactat 
      tgttgtata attctaaat tctaagaac caaaagtgt 
      aaactctta ttataatta attccatta acgttggaa 
      gatattttt tatatgaaa aatatattt tgttaattg 
      tgtttccat aagtagaat ttaaattaa aataaactg 
      aaaccagtc gaacaagtt tcactttat gaacaaaca 
      ataatttat tcatgttta actgtgacc agtggcgtt 
      acttttgta ataaaaaat cttgaaaac accagacca 
      aattctgat gttgatata gttttatta ccttctaat 
      aactaaaat aaagtgatt acaactagt cattgaaac 
      acaatgaaa ttttttatt gctttagta acacgtaaa 
      cgtgaacat cacacaaca taatcaata tttatttta 
      aactaaatg aaaatacac aatgataaa attaaaagt 
      agcaagtct tgaaaacaa gtaaaaatt ttcgaaaac 
      tttcgacgc tcctgatga gttgatgat aatacgcaa 
      acctcgaat acacatcga gttgtttat gttaaaaga 
      aagttaagt tgcacagtc gcagttgag tgtaaaccc 
      tgatagtgc tatgttcgt gtgacgtgt tcgatctct 
      ggtgctaaa gcatcggtg atttgaacg ctttttgga 
      atgcaaccg gaaggaacg ctcgctaac ccgttaacc 
      agcgatggc gaggatacg gtcagtact tcttcagag 
      gatgacaat gagtgtgat ggtgctgtc gtcttacac 
      gaagacgac agaccggaa gtaccgtcg tttggcaat 
      atctcggtg atgaactct aatgatatc cactttgga 
      aataaaacg ttctatcaa ggaccagtg actattaaa 
      cagttctta tacgcgaat ggtaaaacc actagtgac 
      aagagtgat agtgaagtt gtattagat gcgacgaca 
      cttgcagat agtggactt gaaaatccg acgtttgtg 
      aatgataac aatactgcg tcggagaag accaatgct 
      gcgagtaaa cctgcagtg caggaaaat agttcagga 
      attgttaaa ggatttcga cgattacag aaattcttt 
      caagaaaac gttgtgatt ctagtcagc ctcctagca 
      gttttgatt gtatcatta gcaatttta atggttgtg 
      ttattatta agacatagt cctaatccc atcgaagac 
      gatcaaata actagggaa aaattggat gaacaggag 
      aaaaataca ccagtcgac tcgccaata gatccccag 
      gcaaatctg actcttccc cccaaactt aaacttgtc 
      tcccgtctg gaatggctt gcccaacct ccagtccaa 
      cccgccaac cctctcgct gttcccgtc ccctacgtc 
      ataatcctc cacacggcc acagaaaac tgttcctct 
      caagcccaa tgtattttc cacgtccgt ttcatccaa 
      accttccac atcgaaagc cgaagttgg tgggacatt 
      ggatacaat ttcctggtg ggcggcgac ggagaagct 
      tacgaaggc cggggatgg aaatcagaa ggggcacat 
      acgtacgga tacaacgcc aaaagtatc ggaattgcc 
      ttcattggc acttttaat agttttaaa ccaccggag 
      aggcagata acggcttgc aaacaactg attgcaaaa 
      ggggtggaa ttggggttc ataagaaaa gattataag 
      ttgctagcg cacaggcag ctggaaacg acccaaagt 
      ccgggggcg gcgctctat gaggagatg aagacgtgg 
      gagcattgg gcgaaaacg ccgtaattt tgaagatat 
      ttgccataa aaacacgtc caaatttat tgtgatttt 
      tacttagaa ctaaagagg tcaattcta actctactc 
      gtaactgtt ttcaatatt ttaataagt ttaagataa 
      gactgatat tttattgta ctatcaaaa catgaataa 
      agaagttaa ttttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY