Status
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Gene information
General information
TC002789 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10293681..10299791
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002789
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002789 RA
      MPNSEVVLK SENEDSAVT ELEDLCCSS DASFTPTAI 
      NVENSKDVI IGPVTQFHG PVTIYQNVT QSSASRGTI 
      GNGSANTTD LNGKLVKSE STCSDSPHA EKPKNSFLY 
      SKHVKYLGI SLVVIVILV TTTCVLILP RKTEKSPET 
      PDTNTATPR LPLGPGAII EKKIWGGRA TLNFSKPLP 
      HPTHFVIVS HTVTPTCSD FPACSQRVQ SMQDYHVGN 
      LKSPDIGYN FVIGGDGNA YVGRGWDIR NFHMDDSIG 
      ISFIGNFLH DHLTTEMIS VAKKLLDEG VKSGKLARD 
      YKLVAHNQT FRTESPGPN VYKEIKNWP HFDAGIYYS 
      R
    • >TC002789 RB
      MCPLMKLIR VNSSRYRQK SDVVSRSQS GKLVKSEST 
      CSDSPHAEK PKNSFLYSK HVKYLGISL VVIVILVTT 
      TCVLILPRK TEKSPETPD TNTATPRLP LGPGAIIEK 
      KIWGGRATL NFSKPLPHP THFVIVSHT VTPTCSDFP 
      ACSQRVQSM QDYHVGNLK SPDIGYNFV IGGDGNAYV 
      GRGWDIRNF HMDDSIGIS FIGNFLHDH LTTEMISVA 
      KKLLDEGVK SGKLARDYK LVAHNQTFR TESPGPNVY 
      KEIKNWPHF DAGIYYSR
    CDS sequence
    • >TC002789 RA
      atgcccaat tctgaagta gtgttaaaa agtgaaaat 
      gaagatagt gcagtaacg gaattagaa gatttgtgc 
      tgttctagt gacgctagt tttacacca actgctata 
      aatgttgaa aactcaaaa gatgttatc attgggcct 
      gtaactcaa ttccatgga ccggtcacg atttatcaa 
      aatgtgaca caatcatct gcttctaga ggaactata 
      ggaaatgga tctgctaat acaactgat ctcaatggt 
      aaactcgtc aagtctgaa agcacctgt tccgactct 
      ccccacgct gaaaaacca aaaaactcc tttctatac 
      tccaaacat gtgaaatat ttgggcatt tctctagtc 
      gtaatcgta atcctcgtc accacaact tgcgtttta 
      atactccca cggaaaact gaaaagtct cccgaaact 
      cccgacact aacactgcg actccaaga cttcctttg 
      ggccccggt gccatcatc gagaaaaaa atttgggga 
      ggccgtgcc accttaaac ttctctaaa ccactgcca 
      catcccact cattttgtc atcgtttca cacactgtt 
      accccgact tgtagcgac tttcctgca tgttcgcaa 
      agggtgcag tccatgcag gactaccat gtgggcaat 
      ttgaaaagt cccgatatt gggtacaat ttcgtaatt 
      ggtggagat gggaatgcg tatgttgga cgaggctgg 
      gatatcagg aattttcac atggatgat tcgatcggg 
      attagtttt attgggaat tttcttcat gatcatttg 
      acgacggag atgatcagc gtggcgaag aaattgcta 
      gatgagggg gtaaagagt ggaaaattg gccagggat 
      tataaactg gtagctcac aatcagacg tttagaacg 
      gaaagtccg gggccaaat gtttacaaa gagatcaaa 
      aattggccg cattttgat gcgggaatt tattactcg 
      cgatag
    • >TC002789 RB
      atgtgtcct ctcatgaag ctcattaga gtcaattca 
      agccgatat cggcaaaaa agcgacgtg gtttcgcga 
      agccaaagc ggtaaactc gtcaagtct gaaagcacc 
      tgttccgac tctccccac gctgaaaaa ccaaaaaac 
      tcctttcta tactccaaa catgtgaaa tatttgggc 
      atttctcta gtcgtaatc gtaatcctc gtcaccaca 
      acttgcgtt ttaatactc ccacggaaa actgaaaag 
      tctcccgaa actcccgac actaacact gcgactcca 
      agacttcct ttgggcccc ggtgccatc atcgagaaa 
      aaaatttgg ggaggccgt gccacctta aacttctct 
      aaaccactg ccacatccc actcatttt gtcatcgtt 
      tcacacact gttaccccg acttgtagc gactttcct 
      gcatgttcg caaagggtg cagtccatg caggactac 
      catgtgggc aatttgaaa agtcccgat attgggtac 
      aatttcgta attggtgga gatgggaat gcgtatgtt 
      ggacgaggc tgggatatc aggaatttt cacatggat 
      gattcgatc gggattagt tttattggg aattttctt 
      catgatcat ttgacgacg gagatgatc agcgtggcg 
      aagaaattg ctagatgag ggggtaaag agtggaaaa 
      ttggccagg gattataaa ctggtagct cacaatcag 
      acgtttaga acggaaagt ccggggcca aatgtttac 
      aaagagatc aaaaattgg ccgcatttt gatgcggga 
      atttattac tcgcgatag
    mRNA sequence
    • >TC002789 RA
      taaattaac aatttgttg ctagtacga gaatgtggt 
      acccaatta ttatcgtgt cttgtgaaa catgataca 
      tttacaaag ttttattaa ataaaaatg taagaacac 
      ttacaaaat tagttataa tgcgactaa atgacctta 
      atcagtgcg tctgataat tcagcgttt cacgtattt 
      cctttggga atgcccaat tctgaagta gtgttaaaa 
      agtgaaaat gaagatagt gcagtaacg gaattagaa 
      gatttgtgc tgttctagt gacgctagt tttacacca 
      actgctata aatgttgaa aactcaaaa gatgttatc 
      attgggcct gtaactcaa ttccatgga ccggtcacg 
      atttatcaa aatgtgaca caatcatct gcttctaga 
      ggaactata ggaaatgga tctgctaat acaactgat 
      ctcaatggt aaactcgtc aagtctgaa agcacctgt 
      tccgactct ccccacgct gaaaaacca aaaaactcc 
      tttctatac tccaaacat gtgaaatat ttgggcatt 
      tctctagtc gtaatcgta atcctcgtc accacaact 
      tgcgtttta atactccca cggaaaact gaaaagtct 
      cccgaaact cccgacact aacactgcg actccaaga 
      cttcctttg ggccccggt gccatcatc gagaaaaaa 
      atttgggga ggccgtgcc accttaaac ttctctaaa 
      ccactgcca catcccact cattttgtc atcgtttca 
      cacactgtt accccgact tgtagcgac tttcctgca 
      tgttcgcaa agggtgcag tccatgcag gactaccat 
      gtgggcaat ttgaaaagt cccgatatt gggtacaat 
      ttcgtaatt ggtggagat gggaatgcg tatgttgga 
      cgaggctgg gatatcagg aattttcac atggatgat 
      tcgatcggg attagtttt attgggaat tttcttcat 
      gatcatttg acgacggag atgatcagc gtggcgaag 
      aaattgcta gatgagggg gtaaagagt ggaaaattg 
      gccagggat tataaactg gtagctcac aatcagacg 
      tttagaacg gaaagtccg gggccaaat gtttacaaa 
      gagatcaaa aattggccg cattttgat gcgggaatt 
      tattactcg cgatagtga caggaagtg aaataaaat 
      tgtgttaat tgttagtcg ctattgttg atttagtat 
      ttataataa aatacacaa cttgtgaat tgataaatt 
      gtgctattt gtgtaaata aattggcta atatttt
    • >TC002789 RB
      gctaaacgg aaggccgcg ttctttttc agtaactaa 
      accgaagaa cggttcagt cgcattaat ttcagcatc 
      caaatcgaa atttgcgtc aatgagtat gacagtaat 
      gtgtcctct catgaagct cattagagt caattcaag 
      ccgatatcg gcaaaaaag cgacgtggt ttcgcgaag 
      ccaaagcgg taaactcgt caagtctga aagcacctg 
      ttccgactc tccccacgc tgaaaaacc aaaaaactc 
      ctttctata ctccaaaca tgtgaaata tttgggcat 
      ttctctagt cgtaatcgt aatcctcgt caccacaac 
      ttgcgtttt aatactccc acggaaaac tgaaaagtc 
      tcccgaaac tcccgacac taacactgc gactccaag 
      acttccttt gggccccgg tgccatcat cgagaaaaa 
      aatttgggg aggccgtgc caccttaaa cttctctaa 
      accactgcc acatcccac tcattttgt catcgtttc 
      acacactgt taccccgac ttgtagcga ctttcctgc 
      atgttcgca aagggtgca gtccatgca ggactacca 
      tgtgggcaa tttgaaaag tcccgatat tgggtacaa 
      tttcgtaat tggtggaga tgggaatgc gtatgttgg 
      acgaggctg ggatatcag gaattttca catggatga 
      ttcgatcgg gattagttt tattgggaa ttttcttca 
      tgatcattt gacgacgga gatgatcag cgtggcgaa 
      gaaattgct agatgaggg ggtaaagag tggaaaatt 
      ggccaggga ttataaact ggtagctca caatcagac 
      gtttagaac ggaaagtcc ggggccaaa tgtttacaa 
      agagatcaa aaattggcc gcattttga tgcgggaat 
      ttattactc gcgatagtg acaggaagt gaaataaaa 
      ttgtgttaa ttgttagtc gctattgtt gatttagta 
      tttataata aaatacaca acttgtgaa ttgataaat 
      tgtgctatt tgtgtaaat aaattggct aatatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY