| General information | |||
| TC002784 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 10358809..10370042 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002784 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00441 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupation delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
remarks: later than schedule, hours 24
- >TC002784 RA
MDRMPYMSD PTLVPRVQK YLESNVDKT FVDINVMAD DLQRSYREY ARLKRNAFR AQVKKAYSI VLQSYGLED QDNSSSEDE DDSEDSAPE ASTFHNNSL NNQLVNLYN RPQVPKQID ENELIDISS DDSEAPAYN DKPSNGPST SQKESQALP TYQNNTNSK KVDANKDRY QYFGRKRKA ETVGLTPYQ STPKKKKSL ATLQEPSVS FKDIGGMDK ILEDVCKLL IHVRHPEVY RQIGISPPR GFLLHGPPG CGKTLLANA IAGEIGVPL LKVAAPELV AGVSGESEE RIRELFERA IFSTPCILF IDEIDAITP NRQNAQKEM ERRIVAQLL SCLDDLSQN ECGDRVLVI GATNRPDAI DPALRRAGR FDREICLGI PDVQARVQI LKVLTAKLK LSEDFDYDF LAKHTPGYV GADLMSLTR EAAMAAVNR MLNKLKEQH KLAQIVAAE KALEEKQKQ ETAKEETNE VNKSDPEII VDDDPKTEP PKESPIVVI DEEVKQNDT PSTSEAVNG HLLAEEKTP PEEPMPVDV EKQNNPLKD LMMWLHDKS PLSSEELSS LCITMEDFN QALKCVQPS AKREGFATV PDVSWDDVG SLNSVREEL QMAILAPIR HIEHFKELG LNTPTGVLL CGPPGCGKT LLAKAMANE AGINFISVK GPELLNMYV GESERAVRV CFERARNSA PCVIFFDEL DAICPKRSD SREGGATMR VVNQMLTEM DGVQDRQGV YLLAASNRP DIVDPAVLR PGRFDKILF VGLPTASDR IEILRAITR NGTRPRLAP DVDLEAIAS SEQCRGYTG ADLAALVKE AGIVALKEF MLCGDTQKS LVVNMEHFT RAIAKIRPS VPEKDQKHY EKLRKMYAA VPENEVEEM EYS
- >TC002784 RA
atggacaga atgccctac atgtctgac cctacttta gttcctaga gtacaaaag tatttggag tccaatgtt gacaaaacc ttcgtcgat atcaatgtt atggcagat gacttgcaa cggagctat cgcgaatac gcccgactg aaacgaaac gcctttagg gcacaagtg aaaaaagcc tattcaata gtcctccaa agctatggc ttggaggac caagacaac agctcaagt gaagacgag gacgattcc gaggacagt gcccctgaa gcgagcact ttccataat aactccctt aacaaccag ttggtgaat ttgtacaac cgaccacaa gtccccaaa caaatcgac gaaaatgaa ctgattgac ataagtagt gacgattca gaagcacca gcttacaat gataagccc agtaacggc ccttcgaca tcacaaaag gagtcccaa gccctacca acgtaccaa aacaacaca aatagtaaa aaagtcgat gctaacaaa gaccggtat cagtatttc ggccgcaaa cgcaaagcc gagaccgtg ggtctcacc ccctatcag agtacccca aaaaagaaa aaatccttg gccacttta caggaacct tcagtgtct ttcaaagac atagggggc atggataaa atcctggag gatgtttgc aaattgttg atccatgta cgccacccg gaggtttac agacagatc gggatctcg cccccgagg gggttcctc ctgcacggg cctcccggg tgcggcaag acgctccta gccaacgcc atcgcaggg gagatcggc gttcctctg ttgaaagtc gcggccccg gaactagtg gccggtgtt tccggtgaa agcgaggag cgaatccgg gagcttttc gaacgagcc attttctcc accccttgt atcctattt atcgatgaa atcgatgcg attacgcca aatcggcaa aatgcccaa aaggagatg gagaggcga attgttgct caattgctt agttgcctt gatgacttg agtcagaat gaatgtggg gacagagtt ctggttatt ggtgctact aatcgacca gatgctata gacccggcc ttaagacgc gccggtcgt tttgatcgc gaaatctgt ctgggaatc cccgatgtc caagccagg gtccaaatt ttgaaggtt ttgactgcg aaattgaaa ctgagtgaa gatttcgat tatgacttt ctggccaaa catacgccg ggatatgtg ggggctgat ttgatgtcg ctgacaagg gaggcggcc atggcggcg gttaataga atgttgaac aagctcaaa gagcaacac aaactcgca caaatcgtg gcagcggag aaagccctc gaagaaaaa cagaaacaa gagactgcg aaagaagaa acaaacgaa gtgaataag agcgatccc gagatcata gtcgacgac gatcctaaa accgaacca ccgaaagaa tctccgatt gtggtaatc gatgaggaa gtgaaacaa aatgacaca ccttcgact tctgaagcc gtcaatggt cacctttta gctgaagaa aaaacacca cctgaagag ccaatgcct gtcgacgtg gaaaaacaa aacaatcca cttaaagac ttgatgatg tggttgcac gacaaatcg ccattatcc agcgaagaa ttatcatcg ttatgtatc acaatggag gattttaat caagcatta aagtgtgtc caaccttcg gcaaaaaga gagggtttt gcgacggtg cctgatgtt agttgggac gatgtcgga tcactaaat tcagtcaga gaggagctc caaatggct attttagcc cccataaga cacattgaa catttcaaa gaactgggc ttgaacaca cccaccggt gttttattg tgtggaccg ccaggttgt ggcaaaaca ctcctagct aaagctatg gccaacgag gccggtatt aactttatt tcagtcaaa ggaccagaa ctactaaac atgtatgtg ggtgagagc gagcgggcg gtgcgtgtg tgtttcgaa cgggcacga aactccgcc ccttgtgtc atatttttc gacgaattg gacgccatc tgccccaaa agatcggac agcagagag ggcggggct acgatgcgt gtcgttaat caaatgttg accgaaatg gacggtgta caagaccga caaggcgtt tacctcctt gctgccagt aacagacct gacattgtg gatcctgca gtcttaagg ccgggccgt tttgacaaa atcttgttt gtcggtttg cctacagca agtgaccga attgagatt ttgagggct attacccga aacggtaca agaccgcgt ctcgccccc gacgtcgat cttgaagct atagcctct tcggagcaa tgtcggggg tacacaggg gctgatttg gccgcgttg gttaaggaa gccggaatt gtggctttg aaggagttt atgttgtgt ggggacaca cagaagtct ttggttgtt aatatggaa cattttact agagctatt gcgaaaatt agaccatct gtgcctgag aaggatcaa aaacattat gaaaagttg cggaagatg tatgccgcg gttcctgaa aatgaagtt gaggagatg gaatattct taa
- >TC002784 RA
agacgcaaa tttccgggg aaaacaatg tgactccac cacattttt gccaatcaa taacaaaaa cattcctcg gttactcac ctggtggga gtctaataa tcaccaaca aagtcccat atcacaagc aaagaataa tttcaccta cactggcac cgaccatgg acccaactg aaaccatgg cacggcatg tccacacgt aattcaaac ccaggtaaa atggtaaaa tggccaccc acagacaca aagacccac aaaacaccc aaatatatt acatccctt gattttcac gcaccacca tcgcaaaaa tacggcata ctacgatat tcccgttat ctgtggacg gcgtttgat tctctgata aaatcccct cctcgacaa tcgagtgaa ggggaaatc aggcaacaa agacagccg gggccccca tgaacgttg gaaagagac ggaacagtc aggtaacaa gtctgtaca cgtcatagg tagcgacca tgggttttg gcgcccatg gtattgtct gctttctat tgtacacta atgccacca ccaccaagg ggcccggaa tggacgcgc aggtagaag cttaagaca caggtgcag gaggaaact cttcgaaat tatttcggg gatttctgg taacaagtg ggcccactt acccattta ttccggtgg tcctgggct ttgatcaac tctattata tcacaatta aaataccgt aatcaccgg aatcggtag taatatcaa gcgtttacg cgccatttt gcgaccgga ataaatgcc tggagaata gtttcagca ttgattttt ccaggggcg tgcgttatc ctgtgatgc aataaggaa ctttgacat ttagagtaa tgaatgttt ccgagaaaa gctcggaga aattcgcgg aactcgaga cgcaatttt ggcttggag atctcgcaa ataaatttg ttccagttt tctcgtatt tatttggaa aattttgcg aaggataat tagacaatc ttggaaata aatttgtct ccggaaaaa atataaaaa atcgatact tcctctatt tgatattta aattacttt ccctatcta ttgtttgtc ttgaattca gttcatcac tttgttttg caatggtgt gacgcttta aataagacg tcatcgtca aagatatga gtcttatca atttattgc acgaaatac ctactcgtt tgcattaaa tcatttgtt tgtacgcca attgcttaa aatgaggaa aattaaaca tctgaagct tattatttg tttggaatg tttaatggc gtacatttt cgccgttct gattaaaaa ctgatttat tgttttcgc ttcagagag gtttatgag gcgcaataa gggtggttg taaatgtct gtcgcttaa aattcttga agtagttta tggctttgg ctatgtttt agctaaatg gtggaccga tttcccgcc atgatcagc acctctagc gaccactct tcgaataag acaaaaatc aacaagacc aaagcaatg attttactg tttgtacag tttgtggtt gtagaattt ttagtgtag tgtttccag tggaaatag ccattagct gctaattga aaaaaaaaa cctgctggt attaaatcg cacctcccc gtaaaatag tgcgcctta actgtaact gtatacaac gtgtgtatg tgtgtgtgt gtgattaaa aaccgtggc acacactgt ataatctaa aattccata attactatg gaatgagtg attttagta gatgatggg aaaaatcaa caataagag cggcgtcga aataatccc cttgaatga ggtaaacca caggaataa tcgttcacg tgtaatcgg tggaaatac tccagatag aaggttaaa aatggacag aatgcccta catgtctga ccctacttt agttcctag agtacaaaa gtatttgga gtccaatgt tgacaaaac cttcgtcga tatcaatgt tatggcaga tgacttgca acggagcta tcgcgaata cgcccgact gaaacgaaa cgcctttag ggcacaagt gaaaaaagc ctattcaat agtcctcca aagctatgg cttggagga ccaagacaa cagctcaag tgaagacga ggacgattc cgaggacag tgcccctga agcgagcac tttccataa taactccct taacaacca gttggtgaa tttgtacaa ccgaccaca agtccccaa acaaatcga cgaaaatga actgattga cataagtag tgacgattc agaagcacc agcttacaa tgataagcc cagtaacgg cccttcgac atcacaaaa ggagtccca agccctacc aacgtacca aaacaacac aaatagtaa aaaagtcga tgctaacaa agaccggta tcagtattt cggccgcaa acgcaaagc cgagaccgt gggtctcac cccctatca gagtacccc aaaaaagaa aaaatcctt ggccacttt acaggaacc ttcagtgtc tttcaaaga cataggggg catggataa aatcctgga ggatgtttg caaattgtt gatccatgt acgccaccc ggaggttta cagacagat cgggatctc gcccccgag ggggttcct cctgcacgg gcctcccgg gtgcggcaa gacgctcct agccaacgc catcgcagg ggagatcgg cgttcctct gttgaaagt cgcggcccc ggaactagt ggccggtgt ttccggtga aagcgagga gcgaatccg ggagctttt cgaacgagc cattttctc caccccttg tatcctatt tatcgatga aatcgatgc gattacgcc aaatcggca aaatgccca aaaggagat ggagaggcg aattgttgc tcaattgct tagttgcct tgatgactt gagtcagaa tgaatgtgg ggacagagt tctggttat tggtgctac taatcgacc agatgctat agacccggc cttaagacg cgccggtcg ttttgatcg cgaaatctg tctgggaat ccccgatgt ccaagccag ggtccaaat tttgaaggt tttgactgc gaaattgaa actgagtga agatttcga ttatgactt tctggccaa acatacgcc gggatatgt gggggctga tttgatgtc gctgacaag ggaggcggc catggcggc ggttaatag aatgttgaa caagctcaa agagcaaca caaactcgc acaaatcgt ggcagcgga gaaagccct cgaagaaaa acagaaaca agagactgc gaaagaaga aacaaacga agtgaataa gagcgatcc cgagatcat agtcgacga cgatcctaa aaccgaacc accgaaaga atctccgat tgtggtaat cgatgagga agtgaaaca aaatgacac accttcgac ttctgaagc cgtcaatgg tcacctttt agctgaaga aaaaacacc acctgaaga gccaatgcc tgtcgacgt ggaaaaaca aaacaatcc acttaaaga cttgatgat gtggttgca cgacaaatc gccattatc cagcgaaga attatcatc gttatgtat cacaatgga ggattttaa tcaagcatt aaagtgtgt ccaaccttc ggcaaaaag agagggttt tgcgacggt gcctgatgt tagttggga cgatgtcgg atcactaaa ttcagtcag agaggagct ccaaatggc tattttagc ccccataag acacattga acatttcaa agaactggg cttgaacac acccaccgg tgttttatt gtgtggacc gccaggttg tggcaaaac actcctagc taaagctat ggccaacga ggccggtat taactttat ttcagtcaa aggaccaga actactaaa catgtatgt gggtgagag cgagcgggc ggtgcgtgt gtgtttcga acgggcacg aaactccgc cccttgtgt catattttt cgacgaatt ggacgccat ctgccccaa aagatcgga cagcagaga gggcggggc tacgatgcg tgtcgttaa tcaaatgtt gaccgaaat ggacggtgt acaagaccg acaaggcgt ttacctcct tgctgccag taacagacc tgacattgt ggatcctgc agtcttaag gccgggccg ttttgacaa aatcttgtt tgtcggttt gcctacagc aagtgaccg aattgagat tttgagggc tattacccg aaacggtac aagaccgcg tctcgcccc cgacgtcga tcttgaagc tatagcctc ttcggagca atgtcgggg gtacacagg ggctgattt ggccgcgtt ggttaagga agccggaat tgtggcttt gaaggagtt tatgttgtg tggggacac acagaagtc tttggttgt taatatgga acattttac tagagctat tgcgaaaat tagaccatc tgtgcctga gaaggatca aaaacatta tgaaaagtt gcggaagat gtatgccgc ggttcctga aaatgaagt tgaggagat ggaatattc ttaagcagt atttgggct ttaataaga ctcgaattt cgaacttta gtatcggca gtgaatcat ttattgaat tgatttggt actgaagtg cttatgttt agtgttatt tcttagaac aatgtacaa taattaacg atttgttgt ccccaatat tttgtttac ttattaaaa aaatgtagg cgcattaag gcgaactaa ataataaac actacaaat aaatatttt tacgtttta cacaaattg tatttttat attttgtgg tggtaactc gcttaccag ttaagttat gtatcttct atctaaggg tgtcactgc aaagattgt gcaaatagt tgtaaaaaa tttataacg aagaaatat gctaagatt gagtgaaat aaaagttca tactgtag
