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Gene information
General information
TC002784 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10358809..10370042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00441

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    vitellarium shortened - Number of animals showing the phenotype: 4
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupation delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    remarks: later than schedule, hours 24
    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC002784 RA
    CDS sequence
    • >TC002784 RA
      atggacaga atgccctac atgtctgac cctacttta 
      gttcctaga gtacaaaag tatttggag tccaatgtt 
      gacaaaacc ttcgtcgat atcaatgtt atggcagat 
      gacttgcaa cggagctat cgcgaatac gcccgactg 
      aaacgaaac gcctttagg gcacaagtg aaaaaagcc 
      tattcaata gtcctccaa agctatggc ttggaggac 
      caagacaac agctcaagt gaagacgag gacgattcc 
      gaggacagt gcccctgaa gcgagcact ttccataat 
      aactccctt aacaaccag ttggtgaat ttgtacaac 
      cgaccacaa gtccccaaa caaatcgac gaaaatgaa 
      ctgattgac ataagtagt gacgattca gaagcacca 
      gcttacaat gataagccc agtaacggc ccttcgaca 
      tcacaaaag gagtcccaa gccctacca acgtaccaa 
      aacaacaca aatagtaaa aaagtcgat gctaacaaa 
      gaccggtat cagtatttc ggccgcaaa cgcaaagcc 
      gagaccgtg ggtctcacc ccctatcag agtacccca 
      aaaaagaaa aaatccttg gccacttta caggaacct 
      tcagtgtct ttcaaagac atagggggc atggataaa 
      atcctggag gatgtttgc aaattgttg atccatgta 
      cgccacccg gaggtttac agacagatc gggatctcg 
      cccccgagg gggttcctc ctgcacggg cctcccggg 
      tgcggcaag acgctccta gccaacgcc atcgcaggg 
      gagatcggc gttcctctg ttgaaagtc gcggccccg 
      gaactagtg gccggtgtt tccggtgaa agcgaggag 
      cgaatccgg gagcttttc gaacgagcc attttctcc 
      accccttgt atcctattt atcgatgaa atcgatgcg 
      attacgcca aatcggcaa aatgcccaa aaggagatg 
      gagaggcga attgttgct caattgctt agttgcctt 
      gatgacttg agtcagaat gaatgtggg gacagagtt 
      ctggttatt ggtgctact aatcgacca gatgctata 
      gacccggcc ttaagacgc gccggtcgt tttgatcgc 
      gaaatctgt ctgggaatc cccgatgtc caagccagg 
      gtccaaatt ttgaaggtt ttgactgcg aaattgaaa 
      ctgagtgaa gatttcgat tatgacttt ctggccaaa 
      catacgccg ggatatgtg ggggctgat ttgatgtcg 
      ctgacaagg gaggcggcc atggcggcg gttaataga 
      atgttgaac aagctcaaa gagcaacac aaactcgca 
      caaatcgtg gcagcggag aaagccctc gaagaaaaa 
      cagaaacaa gagactgcg aaagaagaa acaaacgaa 
      gtgaataag agcgatccc gagatcata gtcgacgac 
      gatcctaaa accgaacca ccgaaagaa tctccgatt 
      gtggtaatc gatgaggaa gtgaaacaa aatgacaca 
      ccttcgact tctgaagcc gtcaatggt cacctttta 
      gctgaagaa aaaacacca cctgaagag ccaatgcct 
      gtcgacgtg gaaaaacaa aacaatcca cttaaagac 
      ttgatgatg tggttgcac gacaaatcg ccattatcc 
      agcgaagaa ttatcatcg ttatgtatc acaatggag 
      gattttaat caagcatta aagtgtgtc caaccttcg 
      gcaaaaaga gagggtttt gcgacggtg cctgatgtt 
      agttgggac gatgtcgga tcactaaat tcagtcaga 
      gaggagctc caaatggct attttagcc cccataaga 
      cacattgaa catttcaaa gaactgggc ttgaacaca 
      cccaccggt gttttattg tgtggaccg ccaggttgt 
      ggcaaaaca ctcctagct aaagctatg gccaacgag 
      gccggtatt aactttatt tcagtcaaa ggaccagaa 
      ctactaaac atgtatgtg ggtgagagc gagcgggcg 
      gtgcgtgtg tgtttcgaa cgggcacga aactccgcc 
      ccttgtgtc atatttttc gacgaattg gacgccatc 
      tgccccaaa agatcggac agcagagag ggcggggct 
      acgatgcgt gtcgttaat caaatgttg accgaaatg 
      gacggtgta caagaccga caaggcgtt tacctcctt 
      gctgccagt aacagacct gacattgtg gatcctgca 
      gtcttaagg ccgggccgt tttgacaaa atcttgttt 
      gtcggtttg cctacagca agtgaccga attgagatt 
      ttgagggct attacccga aacggtaca agaccgcgt 
      ctcgccccc gacgtcgat cttgaagct atagcctct 
      tcggagcaa tgtcggggg tacacaggg gctgatttg 
      gccgcgttg gttaaggaa gccggaatt gtggctttg 
      aaggagttt atgttgtgt ggggacaca cagaagtct 
      ttggttgtt aatatggaa cattttact agagctatt 
      gcgaaaatt agaccatct gtgcctgag aaggatcaa 
      aaacattat gaaaagttg cggaagatg tatgccgcg 
      gttcctgaa aatgaagtt gaggagatg gaatattct 
    mRNA sequence
    • >TC002784 RA
      agacgcaaa tttccgggg aaaacaatg tgactccac 
      cacattttt gccaatcaa taacaaaaa cattcctcg 
      gttactcac ctggtggga gtctaataa tcaccaaca 
      aagtcccat atcacaagc aaagaataa tttcaccta 
      cactggcac cgaccatgg acccaactg aaaccatgg 
      cacggcatg tccacacgt aattcaaac ccaggtaaa 
      atggtaaaa tggccaccc acagacaca aagacccac 
      aaaacaccc aaatatatt acatccctt gattttcac 
      gcaccacca tcgcaaaaa tacggcata ctacgatat 
      tcccgttat ctgtggacg gcgtttgat tctctgata 
      aaatcccct cctcgacaa tcgagtgaa ggggaaatc 
      aggcaacaa agacagccg gggccccca tgaacgttg 
      gaaagagac ggaacagtc aggtaacaa gtctgtaca 
      cgtcatagg tagcgacca tgggttttg gcgcccatg 
      gtattgtct gctttctat tgtacacta atgccacca 
      ccaccaagg ggcccggaa tggacgcgc aggtagaag 
      cttaagaca caggtgcag gaggaaact cttcgaaat 
      tatttcggg gatttctgg taacaagtg ggcccactt 
      acccattta ttccggtgg tcctgggct ttgatcaac 
      tctattata tcacaatta aaataccgt aatcaccgg 
      aatcggtag taatatcaa gcgtttacg cgccatttt 
      gcgaccgga ataaatgcc tggagaata gtttcagca 
      ttgattttt ccaggggcg tgcgttatc ctgtgatgc 
      aataaggaa ctttgacat ttagagtaa tgaatgttt 
      ccgagaaaa gctcggaga aattcgcgg aactcgaga 
      cgcaatttt ggcttggag atctcgcaa ataaatttg 
      ttccagttt tctcgtatt tatttggaa aattttgcg 
      aaggataat tagacaatc ttggaaata aatttgtct 
      ccggaaaaa atataaaaa atcgatact tcctctatt 
      tgatattta aattacttt ccctatcta ttgtttgtc 
      ttgaattca gttcatcac tttgttttg caatggtgt 
      gacgcttta aataagacg tcatcgtca aagatatga 
      gtcttatca atttattgc acgaaatac ctactcgtt 
      tgcattaaa tcatttgtt tgtacgcca attgcttaa 
      aatgaggaa aattaaaca tctgaagct tattatttg 
      tttggaatg tttaatggc gtacatttt cgccgttct 
      gattaaaaa ctgatttat tgttttcgc ttcagagag 
      gtttatgag gcgcaataa gggtggttg taaatgtct 
      gtcgcttaa aattcttga agtagttta tggctttgg 
      ctatgtttt agctaaatg gtggaccga tttcccgcc 
      atgatcagc acctctagc gaccactct tcgaataag 
      acaaaaatc aacaagacc aaagcaatg attttactg 
      tttgtacag tttgtggtt gtagaattt ttagtgtag 
      tgtttccag tggaaatag ccattagct gctaattga 
      aaaaaaaaa cctgctggt attaaatcg cacctcccc 
      gtaaaatag tgcgcctta actgtaact gtatacaac 
      gtgtgtatg tgtgtgtgt gtgattaaa aaccgtggc 
      acacactgt ataatctaa aattccata attactatg 
      gaatgagtg attttagta gatgatggg aaaaatcaa 
      caataagag cggcgtcga aataatccc cttgaatga 
      ggtaaacca caggaataa tcgttcacg tgtaatcgg 
      tggaaatac tccagatag aaggttaaa aatggacag 
      aatgcccta catgtctga ccctacttt agttcctag 
      agtacaaaa gtatttgga gtccaatgt tgacaaaac 
      cttcgtcga tatcaatgt tatggcaga tgacttgca 
      acggagcta tcgcgaata cgcccgact gaaacgaaa 
      cgcctttag ggcacaagt gaaaaaagc ctattcaat 
      agtcctcca aagctatgg cttggagga ccaagacaa 
      cagctcaag tgaagacga ggacgattc cgaggacag 
      tgcccctga agcgagcac tttccataa taactccct 
      taacaacca gttggtgaa tttgtacaa ccgaccaca 
      agtccccaa acaaatcga cgaaaatga actgattga 
      cataagtag tgacgattc agaagcacc agcttacaa 
      tgataagcc cagtaacgg cccttcgac atcacaaaa 
      ggagtccca agccctacc aacgtacca aaacaacac 
      aaatagtaa aaaagtcga tgctaacaa agaccggta 
      tcagtattt cggccgcaa acgcaaagc cgagaccgt 
      gggtctcac cccctatca gagtacccc aaaaaagaa 
      aaaatcctt ggccacttt acaggaacc ttcagtgtc 
      tttcaaaga cataggggg catggataa aatcctgga 
      ggatgtttg caaattgtt gatccatgt acgccaccc 
      ggaggttta cagacagat cgggatctc gcccccgag 
      ggggttcct cctgcacgg gcctcccgg gtgcggcaa 
      gacgctcct agccaacgc catcgcagg ggagatcgg 
      cgttcctct gttgaaagt cgcggcccc ggaactagt 
      ggccggtgt ttccggtga aagcgagga gcgaatccg 
      ggagctttt cgaacgagc cattttctc caccccttg 
      tatcctatt tatcgatga aatcgatgc gattacgcc 
      aaatcggca aaatgccca aaaggagat ggagaggcg 
      aattgttgc tcaattgct tagttgcct tgatgactt 
      gagtcagaa tgaatgtgg ggacagagt tctggttat 
      tggtgctac taatcgacc agatgctat agacccggc 
      cttaagacg cgccggtcg ttttgatcg cgaaatctg 
      tctgggaat ccccgatgt ccaagccag ggtccaaat 
      tttgaaggt tttgactgc gaaattgaa actgagtga 
      agatttcga ttatgactt tctggccaa acatacgcc 
      gggatatgt gggggctga tttgatgtc gctgacaag 
      ggaggcggc catggcggc ggttaatag aatgttgaa 
      caagctcaa agagcaaca caaactcgc acaaatcgt 
      ggcagcgga gaaagccct cgaagaaaa acagaaaca 
      agagactgc gaaagaaga aacaaacga agtgaataa 
      gagcgatcc cgagatcat agtcgacga cgatcctaa 
      aaccgaacc accgaaaga atctccgat tgtggtaat 
      cgatgagga agtgaaaca aaatgacac accttcgac 
      ttctgaagc cgtcaatgg tcacctttt agctgaaga 
      aaaaacacc acctgaaga gccaatgcc tgtcgacgt 
      ggaaaaaca aaacaatcc acttaaaga cttgatgat 
      gtggttgca cgacaaatc gccattatc cagcgaaga 
      attatcatc gttatgtat cacaatgga ggattttaa 
      tcaagcatt aaagtgtgt ccaaccttc ggcaaaaag 
      agagggttt tgcgacggt gcctgatgt tagttggga 
      cgatgtcgg atcactaaa ttcagtcag agaggagct 
      ccaaatggc tattttagc ccccataag acacattga 
      acatttcaa agaactggg cttgaacac acccaccgg 
      tgttttatt gtgtggacc gccaggttg tggcaaaac 
      actcctagc taaagctat ggccaacga ggccggtat 
      taactttat ttcagtcaa aggaccaga actactaaa 
      catgtatgt gggtgagag cgagcgggc ggtgcgtgt 
      gtgtttcga acgggcacg aaactccgc cccttgtgt 
      catattttt cgacgaatt ggacgccat ctgccccaa 
      aagatcgga cagcagaga gggcggggc tacgatgcg 
      tgtcgttaa tcaaatgtt gaccgaaat ggacggtgt 
      acaagaccg acaaggcgt ttacctcct tgctgccag 
      taacagacc tgacattgt ggatcctgc agtcttaag 
      gccgggccg ttttgacaa aatcttgtt tgtcggttt 
      gcctacagc aagtgaccg aattgagat tttgagggc 
      tattacccg aaacggtac aagaccgcg tctcgcccc 
      cgacgtcga tcttgaagc tatagcctc ttcggagca 
      atgtcgggg gtacacagg ggctgattt ggccgcgtt 
      ggttaagga agccggaat tgtggcttt gaaggagtt 
      tatgttgtg tggggacac acagaagtc tttggttgt 
      taatatgga acattttac tagagctat tgcgaaaat 
      tagaccatc tgtgcctga gaaggatca aaaacatta 
      tgaaaagtt gcggaagat gtatgccgc ggttcctga 
      aaatgaagt tgaggagat ggaatattc ttaagcagt 
      atttgggct ttaataaga ctcgaattt cgaacttta 
      gtatcggca gtgaatcat ttattgaat tgatttggt 
      actgaagtg cttatgttt agtgttatt tcttagaac 
      aatgtacaa taattaacg atttgttgt ccccaatat 
      tttgtttac ttattaaaa aaatgtagg cgcattaag 
      gcgaactaa ataataaac actacaaat aaatatttt 
      tacgtttta cacaaattg tatttttat attttgtgg 
      tggtaactc gcttaccag ttaagttat gtatcttct 
      atctaaggg tgtcactgc aaagattgt gcaaatagt 
      tgtaaaaaa tttataacg aagaaatat gctaagatt 
      gagtgaaat aaaagttca tactgtag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY