Status
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Gene information
General information
TC034599 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11921242..11923176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034599
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00436

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034599 RA
      MCLLLVIVT LVTLLLPLH FTTTPSPTT ATTTAVSNY 
      PYVAFLQNS PTNAWCAGT LISNDTVLT AAHCIYGAN 
      TLSVLLGAN DLTEDSNAV KLRSSEFAI HENYTNTTN 
      SYMHDIGLI FLSQSIMLN DKIDTVPLS NSPFFYTTE 
      KTCYVLGWK TSDNNFSET LLEKIVTLR EECDPFKIC 
      AFGENGPFE GDSGGPLIC ESMQIGIVS GGTVEDNQL 
      SIYVDVGSY NDWISEQIL QNSPTFKIS TLIVSSLLT 
      KMSKEAILL IVLALSHYS VCGSNLELI QAHIEPSKV 
      RIIGGDEVV PHSVPYQVG LRINGNAFC GGALISPNY 
      VLTAGHCGE VIRSVEVIL GAHNISNPS EDTQVTITG 
      SKITNHEKY DSATLRNDI CLIQLSEPA PINDNIQAA 
      KLPPSSDSG KSYFDETVT ATGWGLYKD VPFPTTRDM 
      SDVLLKVEV QVSNLTECG MFYNDDDGT YVVDTNLCT 
      SGYRNKGTC NGDSGGPLS LDGVLIGLT SFGTDLCEM 
      CSPSVYTRV VDYLDWIAQ NSDVHID
    CDS sequence
    • >TC034599 RA
      atgtgctta ttacttgtt attgttact cttgtcaca 
      ttacttttg ccactacat tttactaca actccgagt 
      ccaaccact gctaccacc actgcagtt tcgaactac 
      ccatatgtg gccttttta cagaattct cccacaaac 
      gcttggtgt gctggaact ttaatttcg aatgataca 
      gttttaaca gcagctcat tgtatttac ggtgcaaac 
      acgctttca gttctacta ggagctaat gatctaacc 
      gaagattca aatgctgta aagttgaga agttccgaa 
      tttgctatt cacgagaat tatactaat accacaaat 
      tcttatatg catgatatt gggcttatt ttcttaagt 
      cagtcaatt atgcttaat gacaaaatt gatactgtt 
      ccgttgtct aatagtccg tttttctat acaacggag 
      aaaacctgt tatgttttg ggttggaaa acatcagat 
      aacaacttc agtgaaaca ttattagaa aaaatcgtt 
      acactaagg gaagaatgc gatccattc aaaatttgt 
      gcttttggt gaaaatggc ccttttgaa ggagattcg 
      ggaggacct cttatctgc gaatccatg caaattggt 
      atagtttca ggtggaact gtggaggac aatcaactg 
      tcaatatac gtggacgtt ggaagttac aacgattgg 
      atcagtgaa caaatttta caaaatagt cctacgttt 
      aaaatctca accttgatt gtttcatca cttttgacc 
      aaaatgtcc aaagaagca atcctgttg attgtcttg 
      gctctaagc cactatagt gtctgtggt agcaacctg 
      gagctgatt caagcccac attgaacca tcaaaagtc 
      cgaattatt ggaggcgac gaagtagta ccccattcc 
      gtaccttac caggttggt ttaagaatc aatggaaat 
      gccttctgt ggcggagct ctcatttcg ccaaattac 
      gttctcaca gctggtcat tgtggcgaa gtgattcga 
      tcagttgag gtaattttg ggtgctcac aatatttcc 
      aacccttca gaagacact caagttaca ataactggg 
      agcaaaatc acaaaccac gaaaaatat gattctgca 
      acgcttcgt aacgatatt tgtttgatt cagttgtca 
      gaaccagct cctattaat gacaatatt caagcagca 
      aagctacca ccaagtagt gattcgggt aaaagttat 
      tttgatgaa actgtcaca gcaactgga tgggggttg 
      tacaaggat gtacctttt cccaccact agagacatg 
      tctgatgtt ctgttgaaa gttgaggtt caagtttca 
      aatttaact gaatgtgga atgttttac aatgatgat 
      gatggtact tatgtagtt gacacaaac ctttgtacc 
      tcgggatat agaaataaa gggacttgt aatggagat 
      tctggtggt cctttgagt ctagatggg gtacttatt 
      ggacttacc tctttcgga acggatttg tgtgaaatg 
      tgcagtcct tcggtatac acaagagtc gtggattac 
      cttgattgg attgctcaa aattctgat gttcacatt 
      gattga
    mRNA sequence
    • >TC034599 RA
      tgttccaga tttaaccct gtacgctgt gctcaaata 
      accatgtgc ttattactt gttattgtt actcttgtc 
      acattactt ttgccacta cattttact acaactccg 
      agtccaacc actgctacc accactgca gtttcgaac 
      tacccatat gtggccttt ttacagaat tctcccaca 
      aacgcttgg tgtgctgga actttaatt tcgaatgat 
      acagtttta acagcagct cattgtatt tacggtgca 
      aacacgctt tcagttcta ctaggagct aatgatcta 
      accgaagat tcaaatgct gtaaagttg agaagttcc 
      gaatttgct attcacgag aattatact aataccaca 
      aattcttat atgcatgat attgggctt attttctta 
      agtcagtca attatgctt aatgacaaa attgatact 
      gttccgttg tctaatagt ccgtttttc tatacaacg 
      gagaaaacc tgttatgtt ttgggttgg aaaacatca 
      gataacaac ttcagtgaa acattatta gaaaaaatc 
      gttacacta agggaagaa tgcgatcca ttcaaaatt 
      tgtgctttt ggtgaaaat ggccctttt gaaggagat 
      tcgggagga cctcttatc tgcgaatcc atgcaaatt 
      ggtatagtt tcaggtgga actgtggag gacaatcaa 
      ctgtcaata tacgtggac gttggaagt tacaacgat 
      tggatcagt gaacaaatt ttacaaaat agtcctacg 
      tttaaaatc tcaaccttg attgtttca tcacttttg 
      accaaaatg tccaaagaa gcaatcctg ttgattgtc 
      ttggctcta agccactat agtgtctgt ggtagcaac 
      ctggagctg attcaagcc cacattgaa ccatcaaaa 
      gtccgaatt attggaggc gacgaagta gtaccccat 
      tccgtacct taccaggtt ggtttaaga atcaatgga 
      aatgccttc tgtggcgga gctctcatt tcgccaaat 
      tacgttctc acagctggt cattgtggc gaagtgatt 
      cgatcagtt gaggtaatt ttgggtgct cacaatatt 
      tccaaccct tcagaagac actcaagtt acaataact 
      gggagcaaa atcacaaac cacgaaaaa tatgattct 
      gcaacgctt cgtaacgat atttgtttg attcagttg 
      tcagaacca gctcctatt aatgacaat attcaagca 
      gcaaagcta ccaccaagt agtgattcg ggtaaaagt 
      tattttgat gaaactgtc acagcaact ggatggggg 
      ttgtacaag gatgtacct tttcccacc actagagac 
      atgtctgat gttctgttg aaagttgag gttcaagtt 
      tcaaattta actgaatgt ggaatgttt tacaatgat 
      gatgatggt acttatgta gttgacaca aacctttgt 
      acctcggga tatagaaat aaagggact tgtaatgga 
      gattctggt ggtcctttg agtctagat ggggtactt 
      attggactt acctctttc ggaacggat ttgtgtgaa 
      atgtgcagt ccttcggta tacacaaga gtcgtggat 
      taccttgat tggattgct caaaattct gatgttcac 
      attgattga ttaacaata aaacaatga cactcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY