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Gene information
General information
TC032487 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid17: 1355026..1359932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032487
    iBeetle screen iB_00432
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032487 RA
      MSSDFYLEL SENPVRFEA VNQLTNVFF DDSNKHVFA 
      VRSGGVMGV VVKGPTESD KPLNFRMED HGPVLSIKF 
      SLDQKVLAV QRSNTSVEF MNFNGATLD SEYSQSCKK 
      NSNILGFVW SQNNEVAFM TDHGIELYM IIPEKKSLK 
      YLKTTSATV QWFVWCSLN KIALLASAH GSQLQPVLF 
      KPGSISKLP KVETEPGRM ALERDVTLS TLYDIPAVL 
      ILRHQSGPQ SAEVHVHTL SGPGQAAVK THVLKLGLS 
      GRFAINIVD DLILVHHQA SRSSQIFDT ALPGESDGT 
      VRYHTTVAP ARSFKPAQI TLPGLVEPQ SHLCELYSP 
      NWVVFQPNI VIDAKLGCL WHINLCLAE LCSSITDLA 
      TCTQLALKR TNGKPILLK LLLEAIRQP KPPLDKLQE 
      SFNHINNVY RECLEAELQ SHTASPPGA PSLKPPPLS 
      RVQIDQMDM YNEIFQKLE ADKDLGKLE WTLISYMTS 
      LFEQGIPAQ HNLNELLIT TLVKRKKFT ALQQLLQYG 
      VVSDSKPLA CLLLSLGNL HPAASQLAL DMLARLNAI 
      EEIQEILLS EGQVLSALK LGQENANPR KFLQAAQNT 
      GDDGMFHSV LYYFKNTSQ FAGAFSKDE RLNQFVQQY 
      NSTFLEK
    CDS sequence
    • >TC032487 RA
      atgtcaagt gatttttat ttggaattg tctgagaac 
      ccggtccgt tttgaggcc gtcaaccag ctcacaaat 
      gtcttcttc gacgactcg aacaaacac gtgttcgcg 
      gtgcgctcg ggtggcgtt atgggagtg gtggtcaaa 
      ggcccaaca gagtccgac aaaccgctc aattttcga 
      atggaagac cacggcccc gtcctatca ataaaattc 
      tcccttgat cagaaggtt ctggcagtt cagcgctca 
      aacacgtcg gttgagttt atgaatttt aacggagcg 
      acactcgat tcagagtac tcccaaagc tgcaagaag 
      aattcgaac attttgggg ttcgtttgg tcgcaaaat 
      aatgaagtt gcgttcatg accgaccac ggaattgaa 
      ctctacatg atcatcccg gaaaaaaag tcactcaaa 
      taccttaaa accacctcg gcgactgtt cagtggttc 
      gtctggtgc tccttaaac aaaatcgcg cttctggca 
      tcggctcat gggtcgcag ctgcagcct gtgcttttt 
      aaaccgggg tcaataagc aaactgcct aaagttgaa 
      accgaacct ggccgtatg gctttagaa cgagacgta 
      actttgtcc actctgtac gacattccc gctgtttta 
      attctgaga catcagagt ggcccccag agtgctgag 
      gtccatgta cacacctta agtggcccg ggacaagcc 
      gccgttaaa acacatgtc ttgaaattg gggctttct 
      gggagattt gcgattaat atcgttgat gatttgatt 
      ctggtccat catcaggcg tcacgaagc tcgcaaata 
      ttcgacacc gctttaccc ggagaaagc gacggtaca 
      gtaaggtac cacacgaca gtggcgcca gctaggtca 
      tttaaacca gctcaaata actctgcct ggattggtt 
      gaacctcaa tcacacctt tgcgaacta tattctcca 
      aactgggtt gttttccaa ccgaatatt gtaattgat 
      gcaaaactg gggtgtttg tggcacatt aacttgtgc 
      ttagctgag ctgtgtagc tcaatcact gacttagcc 
      acttgtaca caactagcg ctgaagcgg acaaacggc 
      aaaccaata ttactcaaa ctgcttctt gaagctata 
      agacaacct aaaccgcct ttagacaaa ttgcaagaa 
      tccttcaac cacataaac aatgtctat cgagagtgc 
      ctggaggcg gagctgcag tcacacact gcctcccct 
      ccaggggcc ccctccctg aaaccgccc cctctgtca 
      agggtccaa atcgaccaa atggacatg tacaacgaa 
      attttccaa aaattagaa gccgacaaa gatctaggc 
      aagctcgag tggactttg attagttac atgacgtcg 
      cttttcgaa caaggcatt ccagcacag cacaatctg 
      aacgaattg ctgattaca acgctcgtg aagcgaaaa 
      aaattcaca gctttgcaa caattgctc cagtatggg 
      gtggtttcg gattccaaa cccttggct tgcttgctc 
      ttatcactg ggtaatttg cacccagcg gcgtctcag 
      ttggcgctc gatatgttg gcaagactg aacgccatt 
      gaggagata caggagatt ctcttgagt gaggggcag 
      gtcttgtcg gcccttaaa ctggggcaa gaaaatgcc 
      aacccaagg aaatttctc caagcggcc caaaataca 
      ggcgatgac gggatgttt catagcgtt ttgtattat 
      tttaaaaat acgtcgcag ttcgcgggg gcttttagc 
      aaagatgaa agactgaac caattcgta cagcagtac 
      aactcaacg tttttagag aaataa
    mRNA sequence
    • >TC032487 RA
      attgaaaat gtcaagtga tttttattt ggaattgtc 
      tgagaaccc ggtccgttt tgaggccgt caaccagct 
      cacaaatgt cttcttcga cgactcgaa caaacacgt 
      gttcgcggt gcgctcggg tggcgttat gggagtggt 
      ggtcaaagg cccaacaga gtccgacaa accgctcaa 
      ttttcgaat ggaagacca cggccccgt cctatcaat 
      aaaattctc ccttgatca gaaggttct ggcagttca 
      gcgctcaaa cacgtcggt tgagtttat gaattttaa 
      cggagcgac actcgattc agagtactc ccaaagctg 
      caagaagaa ttcgaacat tttggggtt cgtttggtc 
      gcaaaataa tgaagttgc gttcatgac cgaccacgg 
      aattgaact ctacatgat catcccgga aaaaaagtc 
      actcaaata ccttaaaac cacctcggc gactgttca 
      gtggttcgt ctggtgctc cttaaacaa aatcgcgct 
      tctggcatc ggctcatgg gtcgcagct gcagcctgt 
      gctttttaa accggggtc aataagcaa actgcctaa 
      agttgaaac cgaacctgg ccgtatggc tttagaacg 
      agacgtaac tttgtccac tctgtacga cattcccgc 
      tgttttaat tctgagaca tcagagtgg cccccagag 
      tgctgaggt ccatgtaca caccttaag tggcccggg 
      acaagccgc cgttaaaac acatgtctt gaaattggg 
      gctttctgg gagatttgc gattaatat cgttgatga 
      tttgattct ggtccatca tcaggcgtc acgaagctc 
      gcaaatatt cgacaccgc tttacccgg agaaagcga 
      cggtacagt aaggtacca cacgacagt ggcgccagc 
      taggtcatt taaaccagc tcaaataac tctgcctgg 
      attggttga acctcaatc acacctttg cgaactata 
      ttctccaaa ctgggttgt tttccaacc gaatattgt 
      aattgatgc aaaactggg gtgtttgtg gcacattaa 
      cttgtgctt agctgagct gtgtagctc aatcactga 
      cttagccac ttgtacaca actagcgct gaagcggac 
      aaacggcaa accaatatt actcaaact gcttcttga 
      agctataag acaacctaa accgccttt agacaaatt 
      gcaagaatc cttcaacca cataaacaa tgtctatcg 
      agagtgcct ggaggcgga gctgcagtc acacactgc 
      ctcccctcc aggggcccc ctccctgaa accgccccc 
      tctgtcaag ggtccaaat cgaccaaat ggacatgta 
      caacgaaat tttccaaaa attagaagc cgacaaaga 
      tctaggcaa gctcgagtg gactttgat tagttacat 
      gacgtcgct tttcgaaca aggcattcc agcacagca 
      caatctgaa cgaattgct gattacaac gctcgtgaa 
      gcgaaaaaa attcacagc tttgcaaca attgctcca 
      gtatggggt ggtttcgga ttccaaacc cttggcttg 
      cttgctctt atcactggg taatttgca cccagcggc 
      gtctcagtt ggcgctcga tatgttggc aagactgaa 
      cgccattga ggagataca ggagattct cttgagtga 
      ggggcaggt cttgtcggc ccttaaact ggggcaaga 
      aaatgccaa cccaaggaa atttctcca agcggccca 
      aaatacagg cgatgacgg gatgtttca tagcgtttt 
      gtattattt taaaaatac gtcgcagtt cgcgggggc 
      ttttagcaa agatgaaag actgaacca attcgtaca 
      gcagtacaa ctcaacgtt tttagagaa ataactaat 
      aaataacat gtatatatt ttatttgta caaataata 
      aaaatataa atatcac