Status
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Gene information
General information
TC002732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12888157..12894814
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002732
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002732 RA
      MNTNNKLSP TRNFSGNLA DNNEEPYPS LSDYHDYEP 
      NLEFDDSEL HQSPDIVVQ TAELIQMES NGINSPIPD 
      FTDDNFEDE LADPSEENL DSLVYSPSY PEVDDSPTR 
      KFSEIDKHK IVDVKGDDS AGRKIIVVY AHRLPPIAE 
      INHSLFLNY LTHTLDQYV EQDYSLVYF HYGLSSKNK 
      PSLRWLVQA YKAFDRKYK KNLKALYLV HPTGFLKFV 
      SQIFRPLIS AKFGKKINY INTLKELNE HISLEKLDI 
      PEEVIEHDR KLGGSVTPT TDASFQPAS PLQQFGVTL 
      QFIKDNYNV VIPPVVKQC VEYLDQPDA LETEGIFRR 
      SANTLKIRQ LKEAANQGE TLTFADAHE AAVLLKTFL 
      RELKEPLLT YELYDEIIQ FQTWEKDQQ LRQVSILVM 
      EKLPVDNYQ ILKYIISFL SRVMERADL NKMNAQNLA 
      VVFGPNLVW SEIESMSLV AIGPINMFT QFLLQHCNA 
      IFMI
    CDS sequence
    • >TC002732 RA
      atgaatacc aacaacaag ctcagtcca acgcgaaac 
      ttttcaggt aatctcgca gataacaat gaggagccc 
      tacccaagc ctatccgat taccacgac tacgaacct 
      aatctcgaa ttcgacgat agcgagttg catcagagc 
      cctgatatt gttgtccaa acagccgag ttgattcaa 
      atggaatca aacggcatt aactcgccc attcccgac 
      ttcaccgac gacaacttt gaggacgag ttggccgac 
      cccagcgag gagaacttg gacagctta gtgtactcc 
      ccttcctac cccgaagtc gacgactcc cccacacgt 
      aagttttcc gaaattgat aaacataaa atcgtggat 
      gtgaaagga gacgattcc gcggggaga aaaatcatc 
      gttgtgtac gcccatagg ctgccgccc atcgccgag 
      atcaatcac agtctattt ttaaattat cttacccac 
      actttagat cagtatgtg gaacaggat tatagctta 
      gtgtacttc cattatggc ctctcaagc aagaataaa 
      ccctccctc aggtggctt gtgcaagcc tataaggct 
      ttcgatagg aaatataaa aagaatttg aaagctttg 
      tacttagtc catccaact ggcttccta aagttcgtc 
      tcgcaaatc ttcagaccc ttaattagc gccaaattt 
      gggaagaaa atcaattat ataaacact ttgaaagag 
      ttgaatgag cacatatcg ctggaaaag ttggacatt 
      ccggaggaa gtgattgaa cacgatagg aaacttggg 
      ggtagtgta actccaaca actgacgcc agtttccaa 
      cctgcgtca cctttgcaa caatttggg gtgaccctc 
      caattcatt aaagacaat tacaatgta gttatacca 
      ccagttgtc aaacaatgt gttgaatat ttggatcaa 
      cctgatgcc ctagagact gaaggtata tttaggaga 
      tctgcaaat actctaaaa ataagacag ttgaaagaa 
      gccgcaaat caaggcgaa actctcact tttgcggac 
      gctcacgag gctgcagtt ttgcttaaa acgtttctc 
      cgcgagttg aaagaaccc ttactgact tacgaactg 
      tacgacgaa ataatccag tttcaaact tgggaaaaa 
      gaccaacaa ctgcgacaa gtttctatc ctagtcatg 
      gaaaagcta ccagttgat aactatcaa attttgaaa 
      tacattatt agcttcctt tcaagggtg atggaaagg 
      gccgacttg aataaaatg aatgcacaa aacttagct 
      gtggtattc ggcccaaat ctagtgtgg tctgagatc 
      gaatcgatg tcactggtg gccataggc cccataaac 
      atgttcacc cagtttttg ttgcaacac tgtaacgcg 
      atatttatg atttaa
    mRNA sequence
    • >TC002732 RA
      atgccctac gatgcaacc aaaacgcgc aggttatgt 
      tgattgtca aattgtcgg aagacgtca aaaaagtaa 
      tttatcgta atttacatg tttacttac taacattta 
      cgtttttac aattgggag tcgcttccc caacttcta 
      gtctttttt tgtgttttt attttgtgt aattcggtg 
      tttgttttt ttaccggga aagtgggtg tgctaataa 
      aaacgaaaa tttcctttt gaaatgact taatttgca 
      tttaagatg aataccaac aacaagctc agtccaacg 
      cgaaacttt tcaggtaat ctcgcagat aacaatgag 
      gagccctac ccaagccta tccgattac cacgactac 
      gaacctaat ctcgaattc gacgatagc gagttgcat 
      cagagccct gatattgtt gtccaaaca gccgagttg 
      attcaaatg gaatcaaac ggcattaac tcgcccatt 
      cccgacttc accgacgac aactttgag gacgagttg 
      gccgacccc agcgaggag aacttggac agcttagtg 
      tactcccct tcctacccc gaagtcgac gactccccc 
      acacgtaag ttttccgaa attgataaa cataaaatc 
      gtggatgtg aaaggagac gattccgcg gggagaaaa 
      atcatcgtt gtgtacgcc cataggctg ccgcccatc 
      gccgagatc aatcacagt ctattttta aattatctt 
      acccacact ttagatcag tatgtggaa caggattat 
      agcttagtg tacttccat tatggcctc tcaagcaag 
      aataaaccc tccctcagg tggcttgtg caagcctat 
      aaggctttc gataggaaa tataaaaag aatttgaaa 
      gctttgtac ttagtccat ccaactggc ttcctaaag 
      ttcgtctcg caaatcttc agaccctta attagcgcc 
      aaatttggg aagaaaatc aattatata aacactttg 
      aaagagttg aatgagcac atatcgctg gaaaagttg 
      gacattccg gaggaagtg attgaacac gataggaaa 
      cttgggggt agtgtaact ccaacaact gacgccagt 
      ttccaacct gcgtcacct ttgcaacaa tttggggtg 
      accctccaa ttcattaaa gacaattac aatgtagtt 
      ataccacca gttgtcaaa caatgtgtt gaatatttg 
      gatcaacct gatgcccta gagactgaa ggtatattt 
      aggagatct gcaaatact ctaaaaata agacagttg 
      aaagaagcc gcaaatcaa ggcgaaact ctcactttt 
      gcggacgct cacgaggct gcagttttg cttaaaacg 
      tttctccgc gagttgaaa gaaccctta ctgacttac 
      gaactgtac gacgaaata atccagttt caaacttgg 
      gaaaaagac caacaactg cgacaagtt tctatccta 
      gtcatggaa aagctacca gttgataac tatcaaatt 
      ttgaaatac attattagc ttcctttca agggtgatg 
      gaaagggcc gacttgaat aaaatgaat gcacaaaac 
      ttagctgtg gtattcggc ccaaatcta gtgtggtct 
      gagatcgaa tcgatgtca ctggtggcc ataggcccc 
      ataaacatg ttcacccag tttttgttg caacactgt 
      aacgcgata tttatgatt taaaatgct ttaaggagt 
      cgattctgt gattgttat tgcattttt ttcagtaca 
      aaattcgtt ttacattct agatcgcga ccacttacg 
      acagggttt ctgtgtcaa gattaattt gagccataa 
      tcgtgtttt acttaaatt gttatcatc tcatattaa 
      ttaataatg ttgatcata aaatagttt gcctggata 
      tatcatgtt agacgaaag atgtaagag atttaaagt 
      accagtttg tgttgctta ttacgacaa acataagac 
      ttatagaag tagttatgt aactgtaag agcagtaac 
      aaatgtagt aattttgta aattatata taccggtgc 
      atatccttt attgaggaa gagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY