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Gene information
General information
TC002722 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid17: 1825084..1830501
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002722
    iBeetle screen iB_00429
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24 adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002722 RA
      MAVFQRYDF IFRFSKDFH LQKKALDII HNYSNEVIA 
      TKRKELDEN VKQKESVDD LGRKEKMVF LDMILNKAI 
      DGHIFSQEA IRGEVDTFM FAGHDTTAT AISFILLCI 
      ANHNEVQDK IIEEQERLF GCEKNPKVT FAHLQEMQF 
      LENVIKEGL RLYTPVPLY GRQIDKNVE FEGMLIPKG 
      VNVVIFNHG VHMNPEFYP NPEKFDPNR FESMEDKHP 
      FAFIPFSAG PRVCIGKRC PRKYKNTIK
    • >TC002722 RB
      MIVFTGYLI AIVLILFPL YYYYAKCYA LEKFYHQFP 
      SPPKTPFFG NALDLKTTK DLFQSFHRY VKAYGTMVH 
      LKIGPFDHM LLCSNPKFL EFLLTSNTL LKKSPNLRY 
      LVPWIGTGV ATAYGPKWK LHRKLVTPA FHYSVLSQF 
      VNTFESSGN ILVEKISKL CDGQKVDNF DLYPHITRC 
      TFDIICETA MGTKSNVQT NETSVYFES IKEMCRIFM 
      SRTMAVFQR YDFIFRFSK DFHLQKKAL DIIHNYSNE 
      VIATKRKEL DENVKQKES VDDLGRKEK MVFLDMILN 
      KAIDGHIFS QEAIRGEVD TFMFAGHDT TATAISFIL 
      LCIANHNEV QDKIIEEQE RLFGCEKNP KVTFAHLQE 
      MQFLENVIK EGLRLYTPV PLYGRQIDK NVEFEGMLI 
      PKGVNVVIF NHGVHMNPE FYPNPEKFD PNRFESMED 
      KHPFAFIPF SAGPRVCIG KRCPRKYKN TIK
    CDS sequence
    • >TC002722 RA
      atggcagtg tttcaacgt tacgatttt attttccgg 
      ttttcaaaa gactttcat ttgcagaag aaggcgtta 
      gatatcata cataactac tcaaatgaa gtgattgca 
      acaaaacgc aaagaactg gacgaaaat gtaaaacaa 
      aaagagagt gttgacgat ttggggaga aaggaaaag 
      atggttttt ttggacatg attttgaac aaagctatt 
      gatggtcac atctttagc caggaagcg attagagga 
      gaagtggac acttttatg tttgctggt cacgatacc 
      acggccacc gcaataagt ttcattctt ctttgcatc 
      gcaaaccat aacgaagtt caagacaaa attattgaa 
      gaacaggag cgtcttttt ggctgtgaa aaaaaccca 
      aaagtgact tttgcacat ttacaagaa atgcagttt 
      ttggaaaat gttatcaag gaagggttg cgtttgtac 
      actcctgtg ccgctgtac ggaaggcaa attgataaa 
      aatgtggaa tttgaggga atgcttatt cctaaagga 
      gtcaatgtc gttattttt aatcacggg gttcacatg 
      aatcctgaa ttttacccc aatcctgaa aaattcgat 
      ccgaacaga tttgaaagt atggaagat aaacacccg 
      tttgcgttt ataccattc agtgctggt ccacgagtt 
      tgcattggt aaaagatgt ccaagaaag tacaaaaat 
      acgattaaa taa
    • >TC002722 RB
      atgattgtc ttcactggg tatttaatt gccattgtt 
      ttgattttg tttcctctt tattactac tacgcaaaa 
      tgttatgct ttggagaag ttttaccat caatttcca 
      agccctcca aaaacgccc ttttttgga aatgcttta 
      gatttaaaa acaacaaaa gatctattt caaagtttt 
      cacagatat gtgaaagca tacggaacg atggttcac 
      ctaaaaatt ggtcctttt gatcatatg ttactttgt 
      tccaatccg aaatttctt gaattttta ctgaccagt 
      aacacattg ctaaaaaaa tcaccgaat ttaaggtat 
      ttagtgccg tggattggc actggagta gcaactgct 
      tatggacca aaatggaaa ttacacaga aaactcgta 
      acgccggca tttcattat agtgttttg tcccagttt 
      gtaaacact tttgaatcg agtggaaac attttggtc 
      gagaaaata tcaaaatta tgtgatggg caaaaagtt 
      gataatttt gatctttat ccacacatt acgagatgc 
      acttttgat ataatttgt gagactgca atgggcacc 
      aaatcaaac gtccaaacc aacgaaact tccgtttat 
      tttgaaagt atcaaggaa atgtgtaga atttttatg 
      tctcgaacc atggcagtg tttcaacgt tacgatttt 
      attttccgg ttttcaaaa gactttcat ttgcagaag 
      aaggcgtta gatatcata cataactac tcaaatgaa 
      gtgattgca acaaaacgc aaagaactg gacgaaaat 
      gtaaaacaa aaagagagt gttgacgat ttggggaga 
      aaggaaaag atggttttt ttggacatg attttgaac 
      aaagctatt gatggtcac atctttagc caggaagcg 
      attagagga gaagtggac acttttatg tttgctggt 
      cacgatacc acggccacc gcaataagt ttcattctt 
      ctttgcatc gcaaaccat aacgaagtt caagacaaa 
      attattgaa gaacaggag cgtcttttt ggctgtgaa 
      aaaaaccca aaagtgact tttgcacat ttacaagaa 
      atgcagttt ttggaaaat gttatcaag gaagggttg 
      cgtttgtac actcctgtg ccgctgtac ggaaggcaa 
      attgataaa aatgtggaa tttgaggga atgcttatt 
      cctaaagga gtcaatgtc gttattttt aatcacggg 
      gttcacatg aatcctgaa ttttacccc aatcctgaa 
      aaattcgat ccgaacaga tttgaaagt atggaagat 
      aaacacccg tttgcgttt ataccattc agtgctggt 
      ccacgagtt tgcattggt aaaagatgt ccaagaaag 
      tacaaaaat acgattaaa taa
    mRNA sequence
    • >TC002722 RA
      taaacaaga ggttaagtt tgtctggca ctggtatta 
      gttttgatt aaatgtaat cttcagact ttaagacac 
      gcactgaaa ccatgattg tcttcactg ggtatttaa 
      ttgccattg ttttgattt tgtttcctc tttattact 
      actacgcaa aatgttatg ctttggaga agttttacc 
      atcaatttc caagccctc caaaaacgc ccttttttg 
      gaaatgctt tagatttaa aaacaacaa aagatctat 
      ttcaaagtt ttcacagat atgtgaaag catacggaa 
      cgatggttc acctaaaaa ttggtcctt ttgatcata 
      tgttacttt gttccaatc cgaaatttc ttgaatttt 
      tactgacca gtaacacat tgctaaaaa aatcaccga 
      atttaaggt atttagtgc cgtggattg gcactggag 
      tagcaactg cttatggta caagtaatt atagtttat 
      gagaagaat aattatcat tttttaagg accaaaatg 
      gaaattaca cagaaaact cgtaacgcc ggcatttca 
      ttatagtgt tttgtccca gtttgtaaa cacttttga 
      atcgagtgg aaacatttt ggtcgagaa aatatcaaa 
      attatgtga tgggcaaaa agttgataa ttttgatct 
      ttatccaca cattacgag atgcacttt tgatataat 
      ttgtggtag tcttgttat tgttactta catatctta 
      ttttgtttg aatacttta tagagactg caatgggca 
      ccaaatcaa acgtccaaa ccaacgaaa cttccgttt 
      attttgaaa gtatcaagg aaatgtgta gaattttta 
      tgtctcgaa ccatggcag tgtttcaac gttacgatt 
      ttattttcc ggttttcaa aagactttc atttgcaga 
      agaaggcgt tagatatca tacataact actcaaatg 
      aagtgattg caacaaaac gcaaagaac tggacgaaa 
      atgtaaaac aaaaagaga gtgttgacg atttgggga 
      gaaaggaaa agatggttt ttttggaca tgattttga 
      acaaagcta ttgatggtc acatcttta gccaggaag 
      cgattagag gagaagtgg acactttta tgtttgctg 
      gtcacgata ccacggcca ccgcaataa gtttcattc 
      ttctttgca tcgcaaacc ataacgaag ttcaagaca 
      aaattattg aagaacagg agcgtcttt ttggctgtg 
      aaaaaaacc caaaagtga cttttgcac atttacaag 
      aaatgcagt ttttggaaa atgttatca aggaagggt 
      tgcgtttgt acactcctg tgccgctgt acggaaggc 
      aaattgata aaaatgtgg aatttgagg gaatgctta 
      ttcctaaag gagtcaatg tcgttattt ttaatcacg 
      gggttcaca tgaatcctg aattttacc ccaatcctg 
      aaaaattcg atccgaaca gatttgaaa gtatggaag 
      ataaacacc cgtttgcgt ttataccat tcagtgctg 
      gtccacgag tttgcattg gtaaaagat gtccaagaa 
      agtacaaaa atacgatta aataatgtt tcaggtaaa 
      aaatttgcc atcttggaa ataaaaagc atactttca 
      aaaatcgtt cgaaacttt gagctattt cctgcttct 
      ccgagctac gagttggac ctagctgca gagactgtt 
      ctaaagtcc ctcaacggt attcgaatc gggata
    • >TC002722 RB
      taaacaaga ggttaagtt tgtctggca ctggtatta 
      gttttgatt aaatgtaat cttcagact ttaagacac 
      gcactgaaa ccatgattg tcttcactg ggtatttaa 
      ttgccattg ttttgattt tgtttcctc tttattact 
      actacgcaa aatgttatg ctttggaga agttttacc 
      atcaatttc caagccctc caaaaacgc ccttttttg 
      gaaatgctt tagatttaa aaacaacaa aagatctat 
      ttcaaagtt ttcacagat atgtgaaag catacggaa 
      cgatggttc acctaaaaa ttggtcctt ttgatcata 
      tgttacttt gttccaatc cgaaatttc ttgaatttt 
      tactgacca gtaacacat tgctaaaaa aatcaccga 
      atttaaggt atttagtgc cgtggattg gcactggag 
      tagcaactg cttatggac caaaatgga aattacaca 
      gaaaactcg taacgccgg catttcatt atagtgttt 
      tgtcccagt ttgtaaaca cttttgaat cgagtggaa 
      acattttgg tcgagaaaa tatcaaaat tatgtgatg 
      ggcaaaaag ttgataatt ttgatcttt atccacaca 
      ttacgagat gcacttttg atataattt gtgagactg 
      caatgggca ccaaatcaa acgtccaaa ccaacgaaa 
      cttccgttt attttgaaa gtatcaagg aaatgtgta 
      gaattttta tgtctcgaa ccatggcag tgtttcaac 
      gttacgatt ttattttcc ggttttcaa aagactttc 
      atttgcaga agaaggcgt tagatatca tacataact 
      actcaaatg aagtgattg caacaaaac gcaaagaac 
      tggacgaaa atgtaaaac aaaaagaga gtgttgacg 
      atttgggga gaaaggaaa agatggttt ttttggaca 
      tgattttga acaaagcta ttgatggtc acatcttta 
      gccaggaag cgattagag gagaagtgg acactttta 
      tgtttgctg gtcacgata ccacggcca ccgcaataa 
      gtttcattc ttctttgca tcgcaaacc ataacgaag 
      ttcaagaca aaattattg aagaacagg agcgtcttt 
      ttggctgtg aaaaaaacc caaaagtga cttttgcac 
      atttacaag aaatgcagt ttttggaaa atgttatca 
      aggaagggt tgcgtttgt acactcctg tgccgctgt 
      acggaaggc aaattgata aaaatgtgg aatttgagg 
      gaatgctta ttcctaaag gagtcaatg tcgttattt 
      ttaatcacg gggttcaca tgaatcctg aattttacc 
      ccaatcctg aaaaattcg atccgaaca gatttgaaa 
      gtatggaag ataaacacc cgtttgcgt ttataccat 
      tcagtgctg gtccacgag tttgcattg gtaaaagat 
      gtccaagaa agtacaaaa atacgatta aataatgtt 
      tcaggtaaa aaatttgcc atcttggaa ataaaaagc 
      atactttca aaaatcgtt cgaaacttt gagctattt 
      cctgcttct ccgagctac gagttggac ctagctgca 
      gagactgtt ctaaagtcc ctcaacggt attcgaatc 
      gggata