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Gene information
General information
TC002719 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13376113..13391003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002719
    iBeetle screen iB_00428

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: animals lost, adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: animals lost, remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    egg production: number >150% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002719 RA
      MAKEYIKAV IENTIMEEK CELEASESC DDVETEGAS 
      ESNSMGLRS PGGPLRGRS VKEFEEQLS NLKKENFNL 
      KLRIYFLEE RMGVNFNLD KENVVKKNV ELMVEVESL 
      RKEVQEKHD LLCQAVKAM ELEDEEHKK LVTEKEEML 
      TECQQEIED LKTQLLDAK AESEILSAR TQSGASDHY 
      ASQAFSLPD TPSALKELQ DKIKSLEEE LQNEKGNSA 
      AIQIILDQN DTKIKSLSS QLDEATKKL EYRVHELED 
      MSFQLEEAR AKCQGLHKK LEEKSQIVS ELSSENDEF 
      RKRQNLFSA DLEKERKRY EKLRASSEQ KIADLQSKL 
      EASLAELKK SQDLVVSKS PGRRLGYTD ADSFSVQKP 
      ATSPGSPSK DTNLLPTLP SPVVTPTSS PSPSVSFPF 
      DHNLDLKQI IEQLKTERD AQHQKIVKL KAEQMKACK 
      IIKSMIDSK NKTDSEITT LKQQNEELA RELEEVAAK 
      GDRRQPESL SNENSMDEQ SGELIEHYK ALTDELDEK 
      NRILTATLK EKDNQMKKL QQQYEDIVK KIKEKEEHI 
      VDLEFDLLT VSQEKLKEK EEEIERLNG IIVQRNSDL 
      QGLVNKELW EKNREIEKL QKKQASEIE KLRNDLTTK 
      EKQLSLIKE KISELGIEI DIQNGGISV ENNLQNELE 
      KSEKLRLEA NEVCAVLSR RLEELAVFL DSLLKQKSV 
      LGFLGNKEN KRIRHIIDQ SLDLSRTFT MSMIINPDK 
      SLLQLTNIS SLLNDTNQS EASLLPANM SLTYKSHLY 
      KKDEEDNAQ IIQTLRQQI INLKYELQL RDVELNKMS 
      VCDNFTEIS SEKDDEGLN KSNLNTTST TLKYKSENQ 
      SESEAWSEP DRSVSRARI GLMDGSLKS NSSKKSANT 
      STDSTEDEE DRSSRASKK SVLNESRTT ILELHRQVC 
      ELSQKLNEK ESAYCDVLK NNSKLKQEL DNLEIKLND 
      TELKLAQTL QDKIECDRK VQALEEEFR NMNEKDKKL 
      EDKINEIEL EKVEALKVA KVAEEAFEV AKNEINTLE 
      AKLREKQDE MNELEEELR NEYQNQLKE KVKVLEEES 
      LKKINEMQS GAEQEIKNL RNTIQQLQV EYQLNYVKK 
      CEVEEALEE IERLKSEVD SLEERLEEM TGVEESTNK 
      RFSDYEERI NGLRSDLDN ATLQYSEAV LDKTKIANE 
      KALLEQELN RSVLKEIDT KKRLDEVEN ELQELRQAH 
      QRQVSLLQV QKSKLEVRV SELESSNAE LHNRLVKIQ 
      AGCGDNVAS LSGSPNFVN QIVSFRRQH SDNSGYLSE 
      EQFVMDDGR GTRSFSRLV SNQNFEIER HEANSSPDL 
      GIESDHGRF SSLETHLNV QRPLLQTLE LTESMNNLL 
      DVESNHDQD DNCDNVHCC QKTLEMAHE NSDLKRKLL 
      RTRRALEET VTQLTLANQ RKKQVEKTI CKQIHKTSQ 
      VLRKAKANL DSGSETDAY KK
    CDS sequence
    • >TC002719 RA
      atggccaaa gaatatatc aaagccgtg attgaaaac 
      acgataatg gaagaaaag tgcgaactc gaagcgtcc 
      gaatcatgc gacgatgtc gaaacagaa ggcgcgagc 
      gaaagcaat tcaatgggt ctgcgatca cctggtgga 
      ccgctgcgc ggcaggtcg gtcaaagaa ttcgaagag 
      cagctgtcc aatctcaaa aaggaaaat tttaatctc 
      aaactcagg atttatttt ttagaagaa cggatgggt 
      gtcaatttt aatctagac aaggaaaat gtagttaag 
      aaaaacgtt gaattgatg gttgaagtg gaaagtttg 
      agaaaagaa gtgcaggaa aagcatgat ttattgtgt 
      caagctgtg aaggcaatg gagttggaa gatgaagag 
      cacaagaaa ctggtaacc gaaaaggag gagatgctg 
      acggagtgc cagcaagaa attgaagac ttgaagacg 
      cagctactt gatgctaaa gcggaaagt gagattctg 
      tcggccagg acccaatct ggcgcatct gatcactat 
      gcatctcag gcattcagt ttaccagac actccaagt 
      gcactcaaa gagcttcaa gataaaatt aaatcactg 
      gaggaagag ctgcaaaac gaaaaggga aacagtgcc 
      gcaattcaa ataattctc gatcagaac gatacaaaa 
      atcaaaagt ttatcatcc cagttggac gaagcgacg 
      aaaaaactc gagtatcgc gttcatgaa ctcgaagat 
      atgagtttc caactggag gaggccagg gccaagtgc 
      caaggcttg cacaagaag ctcgaggaa aagtcgcag 
      attgtgagc gaattatcg tcggagaac gatgagttc 
      cgcaaacgg caaaacctc ttctcggcc gatttggag 
      aaagagcgg aagcgttac gaaaagttg agggcgagc 
      agtgagcag aaaattgcc gacctccag tcgaaactg 
      gaggcctct ttggccgaa ctgaagaaa agtcaggac 
      cttgtggtg tctaagagt cccggccgc cgcctcgga 
      tatactgat gccgattcc ttttcagtt caaaaacca 
      gcaacatct cctggatct ccgtcgaaa gacacaaac 
      cttcttcca acactccct tcgccggta gtaacgccc 
      acctcctca ccctcaccc tctgtcagc ttccccttc 
      gatcataac ctcgacctt aagcaaatc atcgagcaa 
      ttgaagacc gaacgcgat gcccaacac cagaaaatc 
      gtcaaactc aaagccgaa caaatgaag gcttgtaag 
      ataatcaag agcatgatc gattcgaaa aacaagacc 
      gacagcgaa atcaccacc ttgaagcag caaaacgaa 
      gagttggcg agggaactt gaagaggtg gctgcaaag 
      ggcgacagg agacaaccg gaatctttg tccaatgag 
      aattcgatg gacgaacaa agcggagag ttaattgag 
      cattataaa gcgctcact gacgaactg gatgagaag 
      aacaggatc ttgacggcc actttgaag gaaaaggac 
      aaccaaatg aagaaattg caacagcag tacgaagac 
      atcgtgaaa aaaattaag gaaaaggaa gaacacatt 
      gtcgatctc gaatttgat ttactgaca gtcagtcaa 
      gaaaaactg aaggagaag gaagaagag attgagcga 
      ctcaacgga atcatcgtc caacgcaac tctgacttg 
      caaggactc gtcaataaa gaactttgg gagaaaaat 
      cgggaaatt gaaaaactc caaaagaaa caagcttcg 
      gaaatcgaa aagttgcga aatgacttg accacgaag 
      gaaaaacag ttgagtctg atcaaggag aagatttcc 
      gaacttggg atcgaaatc gacattcag aatggaggg 
      atcagtgtc gagaacaat ttacaaaac gaattagaa 
      aaatccgaa aagttgaga ctggaagcg aacgaagtt 
      tgtgcagtc ttgagccga agacttgaa gaattggct 
      gtgtttctc gactctttg ctgaagcag aaatcagtg 
      ttgggcttt ttgggcaat aaggagaac aagagaata 
      cgtcacatt atcgaccaa agcctggat ttgtccaga 
      acgttcacc atgagcatg atcattaac ccagacaag 
      agtttactc caactcacc aatatttca agtcttttg 
      aacgacact aaccaaagt gaggctagt ttactgccg 
      gccaatatg agtctgacc tacaaaagc cacctgtac 
      aagaaagat gaggaagat aacgcacag atcattcaa 
      accttgcgc caacaaatt attaatctg aagtatgag 
      ctccagtta cgcgacgtg gagttgaat aaaatgagc 
      gtttgtgac aactttacg gaaatctcg tccgaaaaa 
      gacgacgag ggtttgaac aaatcaaac ttgaacaca 
      acttcgacc actttgaag tacaagagt gagaaccag 
      tcggagtct gaagcatgg tctgagcca gaccggtca 
      gtttcacga gctcgtatc gggcttatg gatggaagt 
      ttgaagtca aattcgagc aaaaagtcg gctaacact 
      tcaacagac tcgactgaa gatgaggaa gaccgaagt 
      tcccgtgct tctaagaag agtgtcctt aatgaaagt 
      cgcacaaca atcctggaa ttgcaccga caagtgtgc 
      gaactcagc cagaaattg aacgaaaaa gagtcggct 
      tattgtgac gtgctgaag aacaacagt aaattgaag 
      caagaattg gacaatttg gaaatcaag ttgaatgac 
      accgaactg aaactggcc caaacactg caagataaa 
      atcgaatgt gacaggaag gtgcaagcg cttgaagaa 
      gagtttaga aatatgaac gagaaagat aaaaagttg 
      gaggataaa atcaacgag attgaattg gaaaaggtt 
      gaagcttta aaagttgcg aaagtggcg gaagaagct 
      ttcgaagtt gcgaaaaat gagataaat acacttgaa 
      gccaaactg cgggagaaa caggacgaa atgaacgaa 
      ctggaggag gagctgaga aacgagtac cagaaccag 
      ttaaaggaa aaagtcaag gttttggag gaagaatcg 
      ttgaagaaa attaatgaa atgcaaagt ggtgccgag 
      caagaaatt aagaatttg cgcaacaca attcagcag 
      ttgcaagtc gagtatcag ttgaattat gtgaagaag 
      tgcgaagtt gaggaagct cttgaggag attgaacgg 
      ttgaagagt gaagttgat tcgctggag gaacgtttg 
      gaggaaatg accggagtg gaggaaagc actaataag 
      agattctcc gattatgag gaacgaatc aatggactg 
      cgaagtgat ctagataat gccactttg cagtattcc 
      gaagccgtt ttggacaaa actaaaata gcaaacgag 
      aaggcgctc ctggaacag gaactcaac cgaagcgtt 
      ttgaaggaa attgacacg aagaagcga ctggatgag 
      gtcgaaaat gagcttcaa gagttgcgc caagcccac 
      cagcgccaa gtgtcgtta ctccaagtg caaaaatct 
      aaacttgaa gtgcgcgtt agtgagttg gaatcgtct 
      aatgcggag ttgcacaac agacttgtt aaaattcaa 
      gcgggttgt ggcgataat gtggcgtct ttatccgga 
      agtccgaat tttgtaaat cagattgtt tcgttcaga 
      cgccaacat tctgacaat tccgggtat ttgtctgaa 
      gaacagttc gtaatggac gatgggaga ggcactagg 
      agtttcagt agattggtc tcaaaccag aatttcgag 
      attgagaga cacgaagca aactcttcg cctgattta 
      gggattgag agcgaccat ggcaggttt tcgagtttg 
      gagacgcac ctgaatgta cagaggccg ctgttgcag 
      actttggag ttgacggag tcgatgaac aatctgctg 
      gatgttgag agcaaccac gaccaagac gataactgt 
      gataatgtc cactgctgc cagaaaacc ctggaaatg 
      gcacatgag aacagcgat ttgaaacgg aagttgctg 
      cggacgagg agagcgttg gaggaaaca gtgacccaa 
      ttgacttta gcaaatcaa cggaaaaaa caagtcgaa 
      aaaaccatt tgcaagcag attcataag acgagtcaa 
      gtgttgcgg aaggcgaaa gctaattta gattcaggt 
      tcagagact gatgcgtat aaaaagtaa
    mRNA sequence
    • >TC002719 RA
      gtctaaccc ttgccacca accaccacc aactttttt 
      tgagcaatt acatttgtt tttgttttt gacgttatt 
      tttgacgca tacctaatt tttcgatgt caccattgt 
      aaattgtcc gtaaaattg tccatcata agcttcatg 
      ggtccttgt taatttgag caacgctcc actggaagg 
      caaaatggc caaagaata tatcaaagc cgtgattga 
      aaacacgat aatggaaga aaagtgcga actcgaagc 
      gtccgaatc atgcgacga tgtcgaaac agaaggcgc 
      gagcgaaag caattcaat gggtctgcg atcacctgg 
      tggaccgct gcgcggcag gtcggtcaa agaattcga 
      agagcagct gtccaatct caaaaagga aaattttaa 
      tctcaaact caggattta ttttttaga agaacggat 
      gggtgtcaa ttttaatct agacaagga aaatgtagt 
      taagaaaaa cgttgaatt gatggttga agtggaaag 
      tttgagaaa agaagtgca ggaaaagca tgatttatt 
      gtgtcaagc tgtgaaggc aatggagtt ggaagatga 
      agagcacaa gaaactggt aaccgaaaa ggaggagat 
      gctgacgga gtgccagca agaaattga agacttgaa 
      gacgcagct acttgatgc taaagcgga aagtgagat 
      tctgtcggc caggaccca atctggcgc atctgatca 
      ctatgcatc tcaggcatt cagtttacc agacactcc 
      aagtgcact caaagagct tcaagataa aattaaatc 
      actggagga agagctgca aaacgaaaa gggaaacag 
      tgccgcaat tcaaataat tctcgatca gaacgatac 
      aaaaatcaa aagtttatc atcccagtt ggacgaagc 
      gacgaaaaa actcgagta tcgcgttca tgaactcga 
      agatatgag tttccaact ggaggaggc cagggccaa 
      gtgccaagg cttgcacaa gaagctcga ggaaaagtc 
      gcagattgt gagcgaatt atcgtcgga gaacgatga 
      gttccgcaa acggcaaaa cctcttctc ggccgattt 
      ggagaaaga gcggaagcg ttacgaaaa gttgagggc 
      gagcagtga gcagaaaat tgccgacct ccagtcgaa 
      actggaggc ctctttggc cgaactgaa gaaaagtca 
      ggaccttgt ggtgtctaa gagtcccgg ccgccgcct 
      cggatatac tgatgccga ttccttttc agttcaaaa 
      accagcaac atctcctgg atctccgtc gaaagacac 
      aaaccttct tccaacact cccttcgcc ggtagtaac 
      gcccacctc ctcaccctc accctctgt cagcttccc 
      cttcgatca taacctcga ccttaagca aatcatcga 
      gcaattgaa gaccgaacg cgatgccca acaccagaa 
      aatcgtcaa actcaaagc cgaacaaat gaaggcttg 
      taagataat caagagcat gatcgattc gaaaaacaa 
      gaccgacag cgaaatcac caccttgaa gcagcaaaa 
      cgaagagtt ggcgaggga acttgaaga ggtggctgc 
      aaagggcga caggagaca accggaatc tttgtccaa 
      tgagaattc gatggacga acaaagcgg agagttaat 
      tgagcatta taaagcgct cactgacga actggatga 
      gaagaacag gatcttgac ggccacttt gaaggaaaa 
      ggacaacca aatgaagaa attgcaaca gcagtacga 
      agacatcgt gaaaaaaat taaggaaaa ggaagaaca 
      cattgtcga tctcgaatt tgatttact gacagtcag 
      tcaagaaaa actgaagga gaaggaaga agagattga 
      gcgactcaa cggaatcat cgtccaacg caactctga 
      cttgcaagg actcgtcaa taaagaact ttgggagaa 
      aaatcggga aattgaaaa actccaaaa gaaacaagc 
      ttcggaaat cgaaaagtt gcgaaatga cttgaccac 
      gaaggaaaa acagttgag tctgatcaa ggagaagat 
      ttccgaact tgggatcga aatcgacat tcagaatgg 
      agggatcag tgtcgagaa caatttaca aaacgaatt 
      agaaaaatc cgaaaagtt gagactgga agcgaacga 
      agtttgtgc agtcttgag ccgaagact tgaagaatt 
      ggctgtgtt tctcgactc tttgctgaa gcagaaatc 
      agtgttggg ctttttggg caataagga gaacaagag 
      aatacgtca cattatcga ccaaagcct ggatttgtc 
      cagaacgtt caccatgag catgatcat taacccaga 
      caagagttt actccaact caccaatat ttcaagtct 
      tttgaacga cactaacca aagtgaggc tagtttact 
      gccggccaa tatgagtct gacctacaa aagccacct 
      gtacaagaa agatgagga agataacgc acagatcat 
      tcaaacctt gcgccaaca aattattaa tctgaagta 
      tgagctcca gttacgcga cgtggagtt gaataaaat 
      gagcgtttg tgacaactt tacggaaat ctcgtccga 
      aaaagacga cgagggttt gaacaaatc aaacttgaa 
      cacaacttc gaccacttt gaagtacaa gagtgagaa 
      ccagtcgga gtctgaagc atggtctga gccagaccg 
      gtcagtttc acgagctcg tatcgggct tatggatgg 
      aagtttgaa gtcaaattc gagcaaaaa gtcggctaa 
      cacttcaac agactcgac tgaagatga ggaagaccg 
      aagttcccg tgcttctaa gaagagtgt ccttaatga 
      aagtcgcac aacaatcct ggaattgca ccgacaagt 
      gtgcgaact cagccagaa attgaacga aaaagagtc 
      ggcttattg tgacgtgct gaagaacaa cagtaaatt 
      gaagcaaga attggacaa tttggaaat caagttgaa 
      tgacaccga actgaaact ggcccaaac actgcaaga 
      taaaatcga atgtgacag gaaggtgca agcgcttga 
      agaagagtt tagaaatat gaacgagaa agataaaaa 
      gttggagga taaaatcaa cgagattga attggaaaa 
      ggttgaagc tttaaaagt tgcgaaagt ggcggaaga 
      agctttcga agttgcgaa aaatgagat aaatacact 
      tgaagccaa actgcggga gaaacagga cgaaatgaa 
      cgaactgga ggaggagct gagaaacga gtaccagaa 
      ccagttaaa ggaaaaagt caaggtttt ggaggaaga 
      atcgttgaa gaaaattaa tgaaatgca aagtggtgc 
      cgagcaaga aattaagaa tttgcgcaa cacaattca 
      gcagttgca agtcgagta tcagttgaa ttatgtgaa 
      gaagtgcga agttgagga agctcttga ggagattga 
      acggttgaa gagtgaagt tgattcgct ggaggaacg 
      tttggagga aatgaccgg agtggagga aagcactaa 
      taagagatt ctccgatta tgaggaacg aatcaatgg 
      actgcgaag tgatctaga taatgccac tttgcagta 
      ttccgaagc cgttttgga caaaactaa aatagcaaa 
      cgagaaggc gctcctgga acaggaact caaccgaag 
      cgttttgaa ggaaattga cacgaagaa gcgactgga 
      tgaggtcga aaatgagct tcaagagtt gcgccaagc 
      ccaccagcg ccaagtgtc gttactcca agtgcaaaa 
      atctaaact tgaagtgcg cgttagtga gttggaatc 
      gtctaatgc ggagttgca caacagact tgttaaaat 
      tcaagcggg ttgtggcga taatgtggc gtctttatc 
      cggaagtcc gaattttgt aaatcagat tgtttcgtt 
      cagacgcca acattctga caattccgg gtatttgtc 
      tgaagaaca gttcgtaat ggacgatgg gagaggcac 
      taggagttt cagtagatt ggtctcaaa ccagaattt 
      cgagattga gagacacga agcaaactc ttcgcctga 
      tttagggat tgagagcga ccatggcag gttttcgag 
      tttggagac gcacctgaa tgtacagag gccgctgtt 
      gcagacttt ggagttgac ggagtcgat gaacaatct 
      gctggatgt tgagagcaa ccacgacca agacgataa 
      ctgtgataa tgtccactg ctgccagaa aaccctgga 
      aatggcaca tgagaacag cgatttgaa acggaagtt 
      gctgcggac gaggagagc gttggagga aacagtgac 
      ccaattgac tttagcaaa tcaacggaa aaaacaagt 
      cgaaaaaac catttgcaa gcagattca taagacgag 
      tcaagtgtt gcggaaggc gaaagctaa tttagattc 
      aggttcaga gactgatgc gtataaaaa gtaaccgtt 
      tactttgta tattgtttt taagtttta gtaaataat 
      ttttcttgt ttgtagttt taatttttt tctttaaag 
      taggacgta aatgactgg aatgtgata tttcctgtg 
      tcttcttgt gccaagagt gtacaaaat tgcagtagt 
      ttattgtta atcctatag tttgactcg aaattcgtt 
      tgaatttta aacagtatt gtgatacta taagtgtat 
      ttttgaaat agtctgtac atacatgtg aatttcatc 
      aatataagt ttttgattt tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY