Status
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Gene information
General information
TC002717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13501618..13542972
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002717
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00427

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002717 RA
      MTGRMAGQT INDRLLAAK HSLAGQGLA KSVCKATTE 
      EMIGPKKKH LDYLLHCTN EPNVSIPQL ANLLIERSQ 
      NTNWVVVYK ALITVHHMM CYGNERFTQ YLASSNSTF 
      QLSNFLDKS GVQGILNVR TGYDMSPFI RRYARYLNE 
      KALSYRTVA FDFCKVKRG KDDGMLRTM NADKLLKTL 
      PVLQNQLDA LLEFDCSAN DLTNGVINM CFMLLFKDL 
      IRLFACYND GIINLLEKF FDMNKKQCK EALDIYKKF 
      LIRMDKVAE FLKVAENVG IDKGDIPDL TKAPSSLLD 
      ALEQHLNHL EGKKNSQTN SSQSTSNQK NVKSGVSAL 
      SSTSNAFGN VVANGRFDT PANGIDENL KLQILAEEE 
      AAMNQFKSK VNSPPAGPN PFLNNSSST SSKTTSQSA 
      SEIDLFAAE PVSAPLKTS DDLLQLSNP FADAFAQPQ 
      PVNTAQFMP PNNIWMANG NAFVAQPPS PVVSNGAFV 
      SENNFASVF GNSEPKSSG FDALGGVLQ PVSAGQTTT 
      SPVTNNNNN NNNMNNNTQ TGALLTGDL ESSLASLAE 
      NLTINSRTA PMKGVQWNS PKNGSKTNN WTPQPMSAT 
      TGAGYKPMN QSMSSPPLS MQTTLQPQM YPQLTPMMG 
      MRPIVTSPA MIPQMSPLG VVPNPSSQM GQQSLLFH
    CDS sequence
    • >TC002717 RA
      atgacggga agaatggct ggtcagact ataaacgac 
      cgacttctg gccgctaag cacagtctc gccggtcag 
      ggattagcc aagtccgtt tgtaaagcc acgaccgaa 
      gagatgatt ggccccaaa aagaaacat ttagattat 
      ttacttcac tgcactaac gaaccaaat gtctcaatt 
      ccgcaatta gctaatctt ctcattgaa cgatcacaa 
      aacacaaat tgggtggtt gtatataaa gccctcatc 
      acagttcat cacatgatg tgttacgga aacgaaaga 
      tttacacaa tatttggcg tccagtaac agcacgttt 
      caactgagc aattttctt gataaaagt ggcgttcaa 
      ggaattcta aatgttagg actggttac gacatgtcg 
      ccctttatc agacgatac gctagatat ttaaatgaa 
      aaagcgtta tcgtaccga actgttgcc ttcgatttt 
      tgcaaagtg aaaaggggg aaagatgac ggcatgttg 
      cggaccatg aatgccgat aagcttcta aaaacattg 
      cctgtttta cagaatcag ttagatgcc cttttggag 
      tttgattgt tcagctaat gaccttacc aatggtgtc 
      attaatatg tgttttatg ttattattc aaggatctt 
      ataagactt ttcgcatgc tataatgat ggtattatt 
      aacttatta gaaaaattc ttcgatatg aataaaaag 
      cagtgcaaa gaggctttg gacatttac aaaaagttc 
      ctcattcga atggacaaa gtggccgaa tttttgaaa 
      gttgctgag aacgtcggt attgataaa ggcgacatt 
      cctgacctc actaaagct cccagcagt ttgttagat 
      gcccttgag caacatctg aatcatctc gaaggcaag 
      aagaattcc cagacgaat tcgtctcaa tcgacaagc 
      aatcagaaa aacgtcaaa tcgggcgtt tccgcctta 
      tcctcaacc agcaacgcg ttcggcaac gtcgtcgcc 
      aacggccgt ttcgacacc ccagccaac ggcatcgac 
      gagaacctc aaactccaa atcctcgcc gaagaggaa 
      gccgcaatg aaccaattc aaatcgaaa gtgaattcg 
      cccccagct ggtcccaac ccatttttg aacaattcg 
      tcttcgacg tcgagcaaa accacctcg cagtcggct 
      tccgaaatt gatttgttt gccgccgag cccgtttca 
      gctcctctt aaaacttcg gatgatttg ttgcagttg 
      tctaatcct ttcgcagat gcttttgct cagccccag 
      ccggtcaat accgcacag ttcatgccg ccgaacaat 
      atctggatg gcgaacgga aacgctttt gtggcccaa 
      cccccgagt ccggtggtg tccaatggg gcgttcgtt 
      tcggaaaat aatttcgcc tcggtgttc ggaaactcc 
      gagccgaaa agcagtggc tttgatgct ctcggtggg 
      gtcctccaa cccgtgtca gccggtcag acaaccaca 
      tctcctgtc acaaacaac aacaataac aataataat 
      atgaacaac aacacacag acgggagcg ttgctgaca 
      ggcgatttg gagtccagt cttgcatca ctagccgag 
      aatctcaca attaacagt agaacagcg ccgatgaaa 
      ggtgtgcaa tggaattcg ccgaaaaac ggatccaaa 
      acaaacaat tggactcct cagccgatg tccgcgacc 
      acaggtgct gggtataaa cccatgaat cagtcaatg 
      tcaagtcca ccactcagc atgcaaact actctacag 
      cctcagatg tacccacaa ctgacaccg atgatgggt 
      atgcgtccg attgtaaca agtccagcg atgattccg 
      cagatgagc cctttgggc gtggttcca aatccgtca 
      agtcagatg ggccaacag tcattgctg ttccattga
    mRNA sequence
    • >TC002717 RA
      gacgcaatc cgtaaagtg acagtccgc ggcccgtcg 
      tagtccaga aagtggcaa aggtgcgtc caaagacct 
      gcggtgtgt gactcgtta aagtgcaag tgtaaagtg 
      ctttatgtt ggagtcgac aaagtgaaa aaagtgcaa 
      tatgacggg aagaatggc tggtcagac tataaacga 
      ccgacttct ggccgctaa gcacagtct cgccggtca 
      gggattagc caagtccgt ttgtaaagc cacgaccga 
      agagatgat tggccccaa aaagaaaca tttagatta 
      tttacttca ctgcactaa cgaaccaaa tgtctcaat 
      tccgcaatt agctaatct tctcattga acgatcaca 
      aaacacaaa ttgggtggt tgtatataa agccctcat 
      cacagttca tcacatgat gtgttacgg aaacgaaag 
      atttacaca atatttggc gtccagtaa cagcacgtt 
      tcaactgag caattttct tgataaaag tggcgttca 
      aggaattct aaatgttag gactggtta cgacatgtc 
      gccctttat cagacgata cgctagata tttaaatga 
      aaaagcgtt atcgtaccg aactgttgc cttcgattt 
      ttgcaaagt gaaaagggg gaaagatga cggcatgtt 
      gcggaccat gaatgccga taagcttct aaaaacatt 
      gcctgtttt acagaatca gttagatgc ccttttgga 
      gtttgattg ttcagctaa tgaccttac caatggtgt 
      cattaatat gtgttttat gttattatt caaggatct 
      tataagact tttcgcatg ctataatga tggtattat 
      taacttatt agaaaaatt cttcgatat gaataaaaa 
      gcagtgcaa agaggcttt ggacattta caaaaagtt 
      cctcattcg aatggacaa agtggccga atttttgaa 
      agttgctga gaacgtcgg tattgataa aggcgacat 
      tcctgacct cactaaagc tcccagcag tttgttaga 
      tgcccttga gcaacatct gaatcatct cgaaggcaa 
      gaagaattc ccagacgaa ttcgtctca atcgacaag 
      caatcagaa aaacgtcaa atcgggcgt ttccgcctt 
      atcctcaac cagcaacgc gttcggcaa cgtcgtcgc 
      caacggccg tttcgacac cccagccaa cggcatcga 
      cgagaacct caaactcca aatcctcgc cgaagagga 
      agccgcaat gaaccaatt caaatcgaa agtgaattc 
      gcccccagc tggtcccaa cccattttt gaacaattc 
      gtcttcgac gtcgagcaa aaccacctc gcagtcggc 
      ttccgaaat tgatttgtt tgccgccga gcccgtttc 
      agctcctct taaaacttc ggatgattt gttgcagtt 
      gtctaatcc tttcgcaga tgcttttgc tcagcccca 
      gccggtcaa taccgcaca gttcatgcc gccgaacaa 
      tatctggat ggcgaacgg aaacgcttt tgtggccca 
      acccccgag tccggtggt gtccaatgg ggcgttcgt 
      ttcggaaaa taatttcgc ctcggtgtt cggaaactc 
      cgagccgaa aagcagtgg ctttgatgc tctcggtgg 
      ggtcctcca acccgtgtc agccggtca gacaaccac 
      atctcctgt cacaaacaa caacaataa caataataa 
      tatgaacaa caacacaca gacgggagc gttgctgac 
      aggcgattt ggagtccag tcttgcatc actagccga 
      gaatctcac aattaacag tagaacagc gccgatgaa 
      aggtgtgca atggaattc gccgaaaaa cggatccaa 
      aacaaacaa ttggactcc tcagccgat gtccgcgac 
      cacaggtgc tgggtataa acccatgaa tcagtcaat 
      gtcaagtcc accactcag catgcaaac tactctaca 
      gcctcagat gtacccaca actgacacc gatgatggg 
      tatgcgtcc gattgtaac aagtccagc gatgattcc 
      gcagatgag ccctttggg cgtggttcc aaatccgtc 
      aagtcagat gggccaaca gtcattgct gttccattg 
      aaatgccat gttgtaaat aaattttca agtgttaca 
      atcaagggt acaataata gtataatta tgtacatat 
      ccaattatt acgaaatgt gatgatttt catatctat 
      aaatttttt ataacggct tctgtagtg gttttaaat 
      tttttgtta tcaatattt ataataatt aaattattg 
      ttagtgtat acgtaaaat tgtcaatct gtccagatc 
      gtagttaat tcggtacat gtttgtgtt aattgataa 
      aaattctaa aattgttgt aggtctctg aatgtgatg 
      aatcagttc aattaaagt tttatcaaa cgttgcgca 
      ggtagtgac aaaagtgtt gatcatttt gtggggtca 
      tgggttctc ttattagtg cgtacctat ggtaagttt 
      ccgcccttg ctgcgccac ttgggacta aaagagaca 
      ataaacttt tccattgtt ggtagctct gcaacattg 
      gatttcaaa atgactgta tatttttaa gtaggtgtt 
      aaagaattg ccatgtaat ttttatgaa ttgtaattt 
      gcacaaatc ttcagcact ttaagtaca cattgttgt 
      ttccttacg atttgtttc aatgaggat ttgttatga 
      ttgtgtata taagattcc agatatgta ctcacaaat 
      acaatgtat ttcacaaag tactctata tttacttat 
      taatgtatc taatactgc ttctgtata ctacacatt 
      ttgttaatt ttaatgtgt aataaactg tattatgga 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY