| General information | |||
| TC002712 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 13590607..13595542 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002712 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00426 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
remarks - cuticles: eggs lost,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
remarks: animals lost,
- >TC002712 RA
MQSHPKLME LRPDRSLLN SDFDGYKLS LAEIPTISE QLKVPVDRL VPDVNQYSL LHAKLFALH NHLISENDN ESVYFVDKE LNVQKFSIE SLTHTFCKT TVWTLPLQR ERSFGDYNI SLKFASSQI AVVADGMGY LYVLDRGCR DIDDKWKIL FSGDVTGPD QKFVVTDVV FKEAPKPHL HLLLTSIRQ REANERNST ILHWITLEK SDSWNQVAL RELTVKGFV QYANFERTC DAVYVISDD GCKFTLDSE NEIKKAEEI QVEKKYKWS QTSEDLTIK FPLPENFKK NLVHVTTEP THISLKYEN ETLLTGKLY HQIDPDVTC WTIESSTLV LTLQKCESG LMWPEIVEG GDVFGEYLP DPALVSEIA ERLAPLTSD TEMGPPTGT TFNSQQVEE CDFECDKLT VFERVSRDS HEVTHKINL GSHQVLLSV GLEENQPLA VGIRSDVDT CIWQPTNEN EFRLVHKGT LLALGYIQA SKQQMKFFV ASPDLSYAA ICESTKHIF IYCQNKSIG LNQLRNRTT GKRVNALAQ QQVFSLPSN EEILGIYAS NTCLYVLGE NFITALKL
- >TC002712 RA
atgcagtct cacccgaaa ttaatggaa ttgcgtcct gatcggtca ctactgaac tctgatttt gacggttac aagttgtct ttggccgag atccccaca attagcgag cagttaaaa gtaccagtg gacagatta gtgcctgat gtaaatcag tattcgttg ctccatgct aaactcttc gctcttcac aatcatttg attagcgaa aatgataat gaatcagtt tattttgtt gataaagag ttgaacgtt caaaaattt agcattgaa tcattgaca cacacattc tgtaaaaca accgtatgg acgctacct ttacagaga gaaaggagc tttggggat tacaatatt tccctgaaa tttgcctca agtcagatt gccgttgta gcagatgga atgggctat ctttacgta ctagacagg ggctgcaga gacattgac gataaatgg aagatactc ttttctggg gacgtgaca ggtcctgat cagaagttt gttgtaact gatgtggtt tttaaagag gccccaaaa ccacatttg catttatta ctaaccagt ataagacag agggaagcg aatgagaga aattcaaca attttgcac tggataact ttggaaaaa agcgattcg tggaaccaa gtggctttg agggaacta acagttaaa ggcttcgtc cagtacgca aatttcgaa cgcacttgt gacgcagtt tacgtaatc agcgatgat ggttgcaaa ttcaccctg gattcggaa aatgaaatc aaaaaagcc gaagaaatt caagttgaa aagaaatac aagtggagc caaaccagt gaagatttg accattaaa tttccactt cctgaaaat tttaagaaa aatttagtc cacgtaacc accgaacca acccacatc tcgctcaaa tacgaaaat gagactctc ctaaccggc aaattgtat caccaaata gaccctgat gtgacttgt tggaccatt gagtcaagc actctcgtc ctgacttta caaaagtgt gaatccggc cttatgtgg ccagaaatc gtcgaaggt ggcgacgtt tttggcgaa tatttgcca gatccggct ttagttagc gaaattgcg gagagattg gccccactt acgagcgat accgagatg gggccgccc acagggacc actttcaac agccaacaa gtggaagag tgtgacttt gagtgcgat aaattgact gtgtttgaa cgagtttcg cgcgattcg catgaggtc acccataaa atcaacttg gggtcacat caagtcctg ctaagtgtt gggttggaa gagaaccag ccgttggct gtagggatc cgaagtgat gttgatacg tgtatctgg cagccgaca aatgaaaat gagtttcga ttggttcat aagggtact ttgttagcg ctgggttat attcaggcg tcaaaacaa caaatgaag ttttttgtg gcctctcct gacctgagt tacgccgct atttgcgaa tcgaccaaa catattttt atttattgc caaaataaa agcatcggt ttgaatcag ttaaggaat agaacgact gggaaaaga gtaaacgca ttagcacag caacaagtc ttcagtcta ccttcaaac gaggaaatt ttagggatt tatgcttca aatacttgt ttatacgtg ttgggagaa aatttcata acggcactt aaattgtaa
- >TC002712 RA
cacacagta tttaattac acaagatgc agtctcacc cgaaattaa tggaattgc gtcctgatc ggtcactac tgaactctg attttgacg gttacaagt tgtctttgg ccgagatcc ccacaatta gcgagcagt taaaagtac cagtggaca gattagtgc ctgatgtaa atcagtatt cgttgctcc atgctaaac tcttcgctc ttcacaatc atttgatta gcgaaaatg ataatgaat cagtttatt ttgttgata aagagttga acgttcaaa aatttagca ttgaatcat tgacacaca cattctgta aaacaaccg tatggacgc tacctttac agagagaaa ggagctttg gggattaca atatttccc tgaaatttg cctcaagtc agattgccg ttgtagcag atggaatgg gctatcttt acgtactag acaggggct gcagagaca ttgacgata aatggaaga tactctttt ctggggacg tgacaggtc ctgatcaga agtttgttg taactgatg tggttttta aagaggccc caaaaccac atttgcatt tattactaa ccagtataa gacagaggg aagcgaatg agagaaatt caacaattt tgcactgga taactttgg aaaaaagcg attcgtgga accaagtgg ctttgaggg aactaacag ttaaaggct tcgtccagt acgcaaatt tcgaacgca cttgtgacg cagtttacg taatcagcg atgatggtt gcaaattca ccctggatt cggaaaatg aaatcaaaa aagccgaag aaattcaag ttgaaaaga aatacaagt ggagccaaa ccagtgaag atttgacca ttaaatttc cacttcctg aaaatttta agaaaaatt tagtccacg taaccaccg aaccaaccc acatctcgc tcaaatacg aaaatgaga ctctcctaa ccggcaaat tgtatcacc aaatagacc ctgatgtga cttgttgga ccattgagt caagcactc tcgtcctga ctttacaaa agtgtgaat ccggcctta tgtggccag aaatcgtcg aaggtggcg acgtttttg gcgaatatt tgccagatc cggctttag ttagcgaaa ttgcggaga gattggccc cacttacga gcgataccg agatggggc cgcccacag ggaccactt tcaacagcc aacaagtgg aagagtgtg actttgagt gcgataaat tgactgtgt ttgaacgag tttcgcgcg attcgcatg aggtcaccc ataaaatca acttggggt cacatcaag tcctgctaa gtgttgggt tggaagaga accagccgt tggctgtag ggatccgaa gtgatgttg atacgtgta tctggcagc cgacaaatg aaaatgagt ttcgattgg ttcataagg gtactttgt tagcgctgg gttatattc aggcgtcaa aacaacaaa tgaagtttt ttgtggcct ctcctgacc tgagttacg ccgctattt gcgaatcga ccaaacata tttttattt attgccaaa ataaaagca tcggtttga atcagttaa ggaatagaa cgactggga aaagagtaa acgcattag cacagcaac aagtcttca gtctacctt caaacgagg aaattttag ggatttatg cttcaaata cttgtttat acgtgttgg gagaaaatt tcataacgg cacttaaat tgtaatttt ttattaatt tttgtaaca tttcaataa ataagtttc actctt
