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Gene information
General information
TC002712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13590607..13595542
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002712
    iBeetle screen iB_00426

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002712 RA
      MQSHPKLME LRPDRSLLN SDFDGYKLS LAEIPTISE 
QLKVPVDRL VPDVNQYSL LHAKLFALH NHLISENDN 
ESVYFVDKE LNVQKFSIE SLTHTFCKT TVWTLPLQR 
ERSFGDYNI SLKFASSQI AVVADGMGY LYVLDRGCR 
DIDDKWKIL FSGDVTGPD QKFVVTDVV FKEAPKPHL 
HLLLTSIRQ REANERNST ILHWITLEK SDSWNQVAL 
RELTVKGFV QYANFERTC DAVYVISDD GCKFTLDSE 
NEIKKAEEI QVEKKYKWS QTSEDLTIK FPLPENFKK 
NLVHVTTEP THISLKYEN ETLLTGKLY HQIDPDVTC 
WTIESSTLV LTLQKCESG LMWPEIVEG GDVFGEYLP 
DPALVSEIA ERLAPLTSD TEMGPPTGT TFNSQQVEE 
CDFECDKLT VFERVSRDS HEVTHKINL GSHQVLLSV 
GLEENQPLA VGIRSDVDT CIWQPTNEN EFRLVHKGT 
LLALGYIQA SKQQMKFFV ASPDLSYAA ICESTKHIF 
IYCQNKSIG LNQLRNRTT GKRVNALAQ QQVFSLPSN 
EEILGIYAS NTCLYVLGE NFITALKL
    CDS sequence
    • >TC002712 RA
      atgcagtct cacccgaaa ttaatggaa ttgcgtcct 
gatcggtca ctactgaac tctgatttt gacggttac 
aagttgtct ttggccgag atccccaca attagcgag 
cagttaaaa gtaccagtg gacagatta gtgcctgat 
gtaaatcag tattcgttg ctccatgct aaactcttc 
gctcttcac aatcatttg attagcgaa aatgataat 
gaatcagtt tattttgtt gataaagag ttgaacgtt 
caaaaattt agcattgaa tcattgaca cacacattc 
tgtaaaaca accgtatgg acgctacct ttacagaga 
gaaaggagc tttggggat tacaatatt tccctgaaa 
tttgcctca agtcagatt gccgttgta gcagatgga 
atgggctat ctttacgta ctagacagg ggctgcaga 
gacattgac gataaatgg aagatactc ttttctggg 
gacgtgaca ggtcctgat cagaagttt gttgtaact 
gatgtggtt tttaaagag gccccaaaa ccacatttg 
catttatta ctaaccagt ataagacag agggaagcg 
aatgagaga aattcaaca attttgcac tggataact 
ttggaaaaa agcgattcg tggaaccaa gtggctttg 
agggaacta acagttaaa ggcttcgtc cagtacgca 
aatttcgaa cgcacttgt gacgcagtt tacgtaatc 
agcgatgat ggttgcaaa ttcaccctg gattcggaa 
aatgaaatc aaaaaagcc gaagaaatt caagttgaa 
aagaaatac aagtggagc caaaccagt gaagatttg 
accattaaa tttccactt cctgaaaat tttaagaaa 
aatttagtc cacgtaacc accgaacca acccacatc 
tcgctcaaa tacgaaaat gagactctc ctaaccggc 
aaattgtat caccaaata gaccctgat gtgacttgt 
tggaccatt gagtcaagc actctcgtc ctgacttta 
caaaagtgt gaatccggc cttatgtgg ccagaaatc 
gtcgaaggt ggcgacgtt tttggcgaa tatttgcca 
gatccggct ttagttagc gaaattgcg gagagattg 
gccccactt acgagcgat accgagatg gggccgccc 
acagggacc actttcaac agccaacaa gtggaagag 
tgtgacttt gagtgcgat aaattgact gtgtttgaa 
cgagtttcg cgcgattcg catgaggtc acccataaa 
atcaacttg gggtcacat caagtcctg ctaagtgtt 
gggttggaa gagaaccag ccgttggct gtagggatc 
cgaagtgat gttgatacg tgtatctgg cagccgaca 
aatgaaaat gagtttcga ttggttcat aagggtact 
ttgttagcg ctgggttat attcaggcg tcaaaacaa 
caaatgaag ttttttgtg gcctctcct gacctgagt 
tacgccgct atttgcgaa tcgaccaaa catattttt 
atttattgc caaaataaa agcatcggt ttgaatcag 
ttaaggaat agaacgact gggaaaaga gtaaacgca 
ttagcacag caacaagtc ttcagtcta ccttcaaac 
gaggaaatt ttagggatt tatgcttca aatacttgt 
ttatacgtg ttgggagaa aatttcata acggcactt 
aaattgtaa
    mRNA sequence
    • >TC002712 RA
      cacacagta tttaattac acaagatgc agtctcacc 
cgaaattaa tggaattgc gtcctgatc ggtcactac 
tgaactctg attttgacg gttacaagt tgtctttgg 
ccgagatcc ccacaatta gcgagcagt taaaagtac 
cagtggaca gattagtgc ctgatgtaa atcagtatt 
cgttgctcc atgctaaac tcttcgctc ttcacaatc 
atttgatta gcgaaaatg ataatgaat cagtttatt 
ttgttgata aagagttga acgttcaaa aatttagca 
ttgaatcat tgacacaca cattctgta aaacaaccg 
tatggacgc tacctttac agagagaaa ggagctttg 
gggattaca atatttccc tgaaatttg cctcaagtc 
agattgccg ttgtagcag atggaatgg gctatcttt 
acgtactag acaggggct gcagagaca ttgacgata 
aatggaaga tactctttt ctggggacg tgacaggtc 
ctgatcaga agtttgttg taactgatg tggttttta 
aagaggccc caaaaccac atttgcatt tattactaa 
ccagtataa gacagaggg aagcgaatg agagaaatt 
caacaattt tgcactgga taactttgg aaaaaagcg 
attcgtgga accaagtgg ctttgaggg aactaacag 
ttaaaggct tcgtccagt acgcaaatt tcgaacgca 
cttgtgacg cagtttacg taatcagcg atgatggtt 
gcaaattca ccctggatt cggaaaatg aaatcaaaa 
aagccgaag aaattcaag ttgaaaaga aatacaagt 
ggagccaaa ccagtgaag atttgacca ttaaatttc 
cacttcctg aaaatttta agaaaaatt tagtccacg 
taaccaccg aaccaaccc acatctcgc tcaaatacg 
aaaatgaga ctctcctaa ccggcaaat tgtatcacc 
aaatagacc ctgatgtga cttgttgga ccattgagt 
caagcactc tcgtcctga ctttacaaa agtgtgaat 
ccggcctta tgtggccag aaatcgtcg aaggtggcg 
acgtttttg gcgaatatt tgccagatc cggctttag 
ttagcgaaa ttgcggaga gattggccc cacttacga 
gcgataccg agatggggc cgcccacag ggaccactt 
tcaacagcc aacaagtgg aagagtgtg actttgagt 
gcgataaat tgactgtgt ttgaacgag tttcgcgcg 
attcgcatg aggtcaccc ataaaatca acttggggt 
cacatcaag tcctgctaa gtgttgggt tggaagaga 
accagccgt tggctgtag ggatccgaa gtgatgttg 
atacgtgta tctggcagc cgacaaatg aaaatgagt 
ttcgattgg ttcataagg gtactttgt tagcgctgg 
gttatattc aggcgtcaa aacaacaaa tgaagtttt 
ttgtggcct ctcctgacc tgagttacg ccgctattt 
gcgaatcga ccaaacata tttttattt attgccaaa 
ataaaagca tcggtttga atcagttaa ggaatagaa 
cgactggga aaagagtaa acgcattag cacagcaac 
aagtcttca gtctacctt caaacgagg aaattttag 
ggatttatg cttcaaata cttgtttat acgtgttgg 
gagaaaatt tcataacgg cacttaaat tgtaatttt 
ttattaatt tttgtaaca tttcaataa ataagtttc 
actctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY