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Gene information
General information
TC032506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14335137..14336691
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032506
    iBeetle screen iB_00425

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032506 RA
      MHYVVFPAV LLLTFSLNL SSGAFPNVY GPIKQQPSE 
      NPGLPLILT PLIEQGRIK DALTASRVY FNGFKTIES 
      YSGYFTVNK AYNSNLFFW FFPSQTDYA NAPVVLWLQ 
      GGPGATSLI GLFAENGPF AVMRQHGLK LRKYSWVKT 
      HSVIYIDNP AGTGYSFTN NGFCQNETQ VGLDLYNAL 
      QQFFLLFPA LQKNDFFVS GESYGGKYT PAIAYTIHT 
      KNPTAKLKI NLKGVSIGN GLTDPVHQL DYADYLYQI 
      GLIDSNVRS TVKQYQDQG IKYIQSKDW VKAFQLFDN 
      LLNGDLNNH TSLFKNVTG FDNYFNFLY PIDPSNELI 
      YMGEYIQRD DVRAAIHVG NATFHGESQ EVELNLMTD 
      VMQSVAPWV AELLSHYRV LIYNGQLDI IVAYPLTVN 
      YLQNLNFSA ADEYKKAQR YKWYVDEDL AGYVKQAGN 
      LTEVLVRNA GHMVPADQP KWAFDLISR FTRNKPFHL
    CDS sequence
    • >TC032506 RA
      atgcattat gtggtgttt cccgccgtg ttattatta 
      actttttcg ttaaattta tcatcgggt gcgttcccc 
      aacgtctac ggccccatc aaacaacag ccaagtgaa 
      aacccgggg ttgccccta atcctaacc ccacttatc 
      gaacagggg cggattaaa gacgcttta accgcatcg 
      cgcgtttac ttcaacggt ttcaaaacc atcgaaagt 
      tactccggg tacttcacc gtgaacaaa gcgtacaat 
      tcgaactta tttttctgg tttttcccc tcacaaacc 
      gactatgca aacgcaccg gtcgttttg tggctacaa 
      gggggcccc ggggccacc tcccttatc gggttattc 
      gccgagaac ggccccttt gccgttatg aggcaacat 
      gggctcaaa ttgcgcaag tattcctgg gttaagaca 
      cattcggtc atttatatc gacaatcct gctgggacc 
      ggttacagt tttaccaac aatgggttc tgccaaaat 
      gagacacaa gtggggctt gatttgtac aatgcgctt 
      caacagttc ttcctgtta ttccccgcg ttgcaaaaa 
      aatgatttt ttcgtttcg ggggagtcc tatgggggg 
      aagtacacc ccagcgatt gcgtatact attcatacg 
      aagaacccc acagctaaa ttgaagatt aatttgaag 
      ggggttagt atcgggaac gggttaact gacccagtt 
      catcagctt gattatgcc gattatttg taccaaatt 
      ggtttgatt gactcaaac gtgcgaagt actgtgaag 
      cagtaccaa gaccaaggg attaaatac atccagagt 
      aaagactgg gtcaaggcg tttcaactg tttgataat 
      ctcctcaac ggcgatttg aacaaccac acttccctc 
      ttcaagaac gtgacaggt ttcgacaat tatttcaac 
      ttcttgtac cccattgat ccgtctaat gaactgatt 
      tatatggga gagtacatt cagagagat gatgtgagg 
      gcggcaatt catgtgggg aatgcaacg tttcatggg 
      gaaagtcag gaggttgag ctgaatcta atgacggat 
      gttatgcag tcggttgcc ccctgggtt gcagaattg 
      ctgagtcat tatagagtg ttgatttac aacggacag 
      ttggatata attgtggcg tatcctctt acagttaac 
      tatttgcaa aatttgaat ttctccgct gctgatgag 
      tataaaaaa gcgcaaagg tacaaatgg tacgttgat 
      gaggattta gctgggtat gtgaaacaa gccgggaat 
      ttgacggag gttttggtg cggaatgct gggcatatg 
      gtaccagcg gatcaaccc aaatgggcg tttgatttg 
      atttcaaga tttactcgt aataaaccg tttcacttg 
      taa
    mRNA sequence
    • >TC032506 RA
      attgttaca taacctcca aagtcaatc gtagtttgt 
      gttttaaac gcgtcaaaa tgcattatg tggtgtttc 
      ccgccgtgt tattattaa ctttttcgt taaatttat 
      catcgggtg cgttcccca acgtctacg gccccatca 
      aacaacagc caagtgaaa acccggggt tgcccctaa 
      tcctaaccc cacttatcg aacaggggc ggattaaag 
      acgctttaa ccgcatcgc gcgtttact tcaacggtt 
      tcaaaacca tcgaaagtt actccgggt acttcaccg 
      tgaacaaag cgtacaatt cgaacttat ttttctggt 
      ttttcccct cacaaaccg actatgcaa acgcaccgg 
      tcgttttgt ggctacaag ggggccccg gggccacct 
      cccttatcg ggttattcg ccgagaacg gcccctttg 
      ccgttatga ggcaacatg ggctcaaat tgcgcaagt 
      attcctggg ttaagacac attcggtca tttatatcg 
      acaatcctg ctgggaccg gttacagtt ttaccaaca 
      atgggttct gccaaaatg agacacaag tggggcttg 
      atttgtaca atgcgcttc aacagttct tcctgttat 
      tccccgcgt tgcaaaaaa atgattttt tcgtttcgg 
      gggagtcct atgggggga agtacaccc cagcgattg 
      cgtatacta ttcatacga agaacccca cagctaaat 
      tgaagatta atttgaagg gggttagta tcgggaacg 
      ggttaactg acccagttc atcagcttg attatgccg 
      attatttgt accaaattg gtttgattg actcaaacg 
      tgcgaagta ctgtgaagc agtaccaag accaaggga 
      ttaaataca tccagagta aagactggg tcaaggcgt 
      ttcaactgt ttgataatc tcctcaacg gcgatttga 
      acaaccaca cttccctct tcaagaacg tgacaggtt 
      tcgacaatt atttcaact tcttgtacc ccattgatc 
      cgtctaatg aactgattt atatgggag agtacattc 
      agagagatg atgtgaggg cggcaattc atgtgggga 
      atgcaacgt ttcatgggg aaagtcagg aggttgagc 
      tgaatctaa tgacggatg ttatgcagt cggttgccc 
      cctgggttg cagaattgc tgagtcatt atagagtgt 
      tgatttaca acggacagt tggatataa ttgtggcgt 
      atcctctta cagttaact atttgcaaa atttgaatt 
      tctccgctg ctgatgagt ataaaaaag cgcaaaggt 
      acaaatggt acgttgatg aggatttag ctgggtatg 
      tgaaacaag ccgggaatt tgacggagg ttttggtgc 
      ggaatgctg ggcatatgg taccagcgg atcaaccca 
      aatgggcgt ttgatttga tttcaagat ttactcgta 
      ataaaccgt ttcacttgt aatctgtgt ttataatat 
      tatgtaata cctactttg atatttctt atgtgtata 
      cccaagaac ttttttgtc tctgaaata aacatataa 
      tcttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY