Status
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Gene information
General information
TC002669 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17825894..17853316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002669
    iBeetle screen iB_00423

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002669 RA
      MRLHILVAV GLFCQGLAV LGEDVEVVE AVPGSEETK 
      EHSNEDNDT NPDQLALES LGEQDQPPK KLAYNGPPR 
      GPINLPPRN NQHYPHKPF KNNKPLYDN KNAYNGPPP 
      PPPKQAMDK YGTSGGDIW PAPVPDMPK IISLDVKCE 
      KNLMKVYIG FDKPFYGIV FSKGHYSNV HCVHLPAGL 
      GRTSAHFEI GIHACGTSG NTENGLYGY GAESGSGTY 
      FENIIVIQY DPQVQEVWD QARKLRCTW HDQYEKSVT 
      FRPFPVDML DVVRTDFAG DNVGCWMQI QVGKGPWAS 
      EVSGLVKIG QTMTMVLAI KDDDSKFDM LVRNCMAHD 
      GKRAPIQLV DQKGCVTRP KLMSRFTKI KNFGASASV 
      LSYAHFQAF KFPDSMEVH FQCTIQICR YQCPDQCSE 
      SNNVGLNGI LELAHSAPD AGYGPPPPP PLPIEAYLH 
      GGRPRDERR VRRSVKSDP EKEVGLNRV IRVVSTGDL 
      TFSLDDNNN TTTMVFPAK EELSGPGLI CMTTPGFAA 
      TLVVLLGIL IISCLMSAF LCVRLRPKA AGHAKKATT 
      KSCFYS
    CDS sequence
    • >TC002669 RA
      atgcggttg cacatactc gtcgcagtc ggccttttt 
      tgtcaaggg ctggccgtc ctgggcgaa gacgtcgaa 
      gtggtcgag gccgttccc ggctccgag gagaccaaa 
      gagcacagc aacgaagac aacgacacg aacccggac 
      caactcgcc ctcgagtcc ctgggcgag caggaccag 
      ccccccaag aaactagcc tataacggc ccgccacgc 
      gggcccatc aacctcccg ccccggaac aaccagcac 
      tacccccac aagcccttc aagaacaac aagccgctc 
      tacgacaac aaaaacgcc tacaatggc cccccacca 
      cctcccccg aagcaagcc atggacaag tacggcacc 
      tccgggggg gacatctgg cccgcccct gtgcccgac 
      atgcccaag atcatctcg ctcgacgtc aagtgcgag 
      aaaaacctt atgaaggtc tacatcggg ttcgataaa 
      cccttctac gggattgtc ttcagtaaa gggcactat 
      agcaacgtc cactgcgtc cacctcccg gctggcctt 
      ggccgcacc tccgcccac ttcgaaatc gggatccac 
      gcctgtggc acctccggg aacaccgaa aacgggctc 
      tacggctac ggggccgag tcgggttcc ggcacctac 
      ttcgaaaac atcatcgtg atccagtac gacccccag 
      gtgcaggag gtctgggac caggccagg aaactgcgc 
      tgcacgtgg cacgaccaa tacgaaaaa tcagtcact 
      ttccggccg ttccctgtg gatatgctg gacgtggtc 
      aggacggac tttgcgggt gataacgtc ggctgctgg 
      atgcaaatc caggtcggg aagggtcca tgggctagt 
      gaggtctcc ggtttagtc aagatcggg cagaccatg 
      accatggtc cttgccata aaggacgac gacagtaaa 
      tttgatatg ttggtacgc aattgcatg gcacatgat 
      gggaagagg gcacccatt cagctcgtc gatcaaaag 
      gggtgcgtc acgcggcct aaacttatg tccaggttc 
      accaaaatc aagaatttc ggggcttcg gcctcagtg 
      ttgtcctat gcgcatttc caggctttc aagttcccg 
      gatagtatg gaggtccac ttccagtgc accatccag 
      atttgccgg taccagtgc ccggaccag tgctcggaa 
      tcgaacaac gtgggcctg aacggcatc cttgaactg 
      gcgcacagc gcgcccgac gcgggctac ggcccgccg 
      cccccgccg ccgctcccc atcgaggcg tacttgcac 
      ggggggcgg ccgcgcgac gagcgcagg gtgcggcgg 
      tcggtgaag agcgacccg gagaaggaa gtgggcctg 
      aatcgggtc attcgagtg gtttccacc ggcgacttg 
      accttctcc ctcgacgat aacaacaac accaccacc 
      atggtgttc cccgccaag gaggagctg tccggtccg 
      ggcctgatc tgcatgacc acgcccggg ttcgcggcc 
      acgctggtg gtgctcctg ggcattctg atcatttcg 
      tgcctgatg tcagccttc ctctgtgtg cgactgagg 
      cccaaagcg gcgggacat gcgaaaaaa gcgacgacc 
      aaaagctgt ttctactcg taa
    mRNA sequence
    • >TC002669 RA
      caggattac cacttccgg gggctgccg cgccgacac 
      agaataacc tgctcctgg agaagatcg taaggaaat 
      gcggttgca catactcgt cgcagtcgg ccttttttg 
      tcaagggct ggccgtcct gggcgaaga cgtcgaagt 
      ggtcgaggc cgttcccgg ctccgagga gaccaaaga 
      gcacagcaa cgaagacaa cgacacgaa cccggacca 
      actcgccct cgagtccct gggcgagca ggaccagcc 
      ccccaagaa actagccta taacggccc gccacgcgg 
      gcccatcaa cctcccgcc ccggaacaa ccagcacta 
      cccccacaa gcccttcaa gaacaacaa gccgctcta 
      cgacaacaa aaacgccta caatggccc cccaccacc 
      tcccccgaa gcaagccat ggacaagta cggcacctc 
      cggggggga catctggcc cgcccctgt gcccgacat 
      gcccaagat catctcgct cgacgtcaa gtgcgagaa 
      aaaccttat gaaggtcta catcgggtt cgataaacc 
      cttctacgg gattgtctt cagtaaagg gcactatag 
      caacgtcca ctgcgtcca cctcccggc tggccttgg 
      ccgcacctc cgcccactt cgaaatcgg gatccacgc 
      ctgtggcac ctccgggaa caccgaaaa cgggctcta 
      cggctacgg ggccgagtc gggttccgg cacctactt 
      cgaaaacat catcgtgat ccagtacga cccccaggt 
      gcaggaggt ctgggacca ggccaggaa actgcgctg 
      cacgtggca cgaccaata cgaaaaatc agtcacttt 
      ccggccgtt ccctgtgga tatgctgga cgtggtcag 
      gacggactt tgcgggtga taacgtcgg ctgctggat 
      gcaaatcca ggtcgggaa gggtccatg ggctagtga 
      ggtctccgg tttagtcaa gatcgggca gaccatgac 
      catggtcct tgccataaa ggacgacga cagtaaatt 
      tgatatgtt ggtacgcaa ttgcatggc acatgatgg 
      gaagagggc acccattca gctcgtcga tcaaaaggg 
      gtgcgtcac gcggcctaa acttatgtc caggttcac 
      caaaatcaa gaatttcgg ggcttcggc ctcagtgtt 
      gtcctatgc gcatttcca ggctttcaa gttcccgga 
      tagtatgga ggtccactt ccagtgcac catccagat 
      ttgccggta ccagtgccc ggaccagtg ctcggaatc 
      gaacaacgt gggcctgaa cggcatcct tgaactggc 
      gcacagcgc gcccgacgc gggctacgg cccgccgcc 
      cccgccgcc gctccccat cgaggcgta cttgcacgg 
      ggggcggcc gcgcgacga gcgcagggt gcggcggtc 
      ggtgaagag cgacccgga gaaggaagt gggcctgaa 
      tcgggtcat tcgagtggt ttccaccgg cgacttgac 
      cttctccct cgacgataa caacaacac caccaccat 
      ggtgttccc cgccaagga ggagctgtc cggtccggg 
      cctgatctg catgaccac gcccgggtt cgcggccac 
      gctggtggt gctcctggg cattctgat catttcgtg 
      cctgatgtc agccttcct ctgtgtgcg actgaggcc 
      caaagcggc gggacatgc gaaaaaagc gacgaccaa 
      aagctgttt ctactcgta agacgctca atggctaga 
      ttaagggct acaagcgtg gtcatggga ccaaagctt 
      tattaatca ctcagaggc gaacggtac gaattaatg 
      aagctttgg gtcggaagt tgtgatcag gcattgtat 
      ccgacacgt gtagttgta aggttacct tgaaattgt 
      ttttggatt ttttgacta aatcactcg tacggcaag 
      tgtatagaa tgtgatgta aatacaata gtttgagat 
      ttttgcgga cggtcgtgg tcggttttg gttctagaa 
      taaaaaatt ttagcccta ggtctaggt taggtattt 
      attttattt atttattta tttatccaa aagacaatc 
      attgcagta tgtacctgt atagaaata gtttgtcga 
      aattgtgta ttggtgttt caagcaaaa tgtattaca 
      ttctagcga aataaaaca actattttt gtatatatt 
      tatttatcc cattattta cacagaaca tagcctctt 
      acatatcgt aaattaaca ataacgttt tcataaaat 
      gagcaatta tcagaatga aataaatac gagaaacgt 
      aacaaaaag gaagtctac tctaatccg aagaataat 
      ttaatcgct taactagtt taaccgaat tactgatta 
      atgcttaat aaaaataat gaaaaaaca ataccgaac 
      tggcagtaa ttctggttc catatttat tatgtacac 
      cacaactaa gctaagttt taacaaaaa taaaattaa 
      ctctaatta aaatactca taaggttaa tactgtaca 
      attcattta attatctga cttttaagt ttgctcact 
      tatctataa taaattaat tccaaacta ctattgcct 
      aactaacat attgagctt agcctttga acttgtcat 
      gattgactg tgaagctta tttgagccg ttttcaggt 
      ccaagccgc acaaattac acacaaatt tggcttttc 
      tgtatgtac aactactaa actaactta gtttttagt 
      tggggccag acagacgtg acgtactaa agggtgtcg 
      taatactgt tttgagagg atgtcaaaa aattatcct 
      gcgacgatc cctcctgtc tccaaacca ggagtaggc 
      ggcccctag tctaaccta gcatttata cagggtgaa 
      caaaaaaca t
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