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Gene information
General information
TC002647 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16678219..16699615
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002647
    iBeetle screen iB_00419

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 4
    previtellogenic egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    egg production: number no/almost no eggs -
    tropharium shape irregular - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4
    mature egg degenerated - Number of animals showing the phenotype: 4
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002647 RA
      MSKQKDLRF LVYLAKIAY PRHNSYCFI IYMIVNYCD 
      ILKQQQIFT LRGVSSEFL NNFVGGTVL DIDNTIVAN 
      DNVEQTCIK YVVVKDTTR KVNLITKKF DTKFVALLS 
      YNLLYNLKG AITCSFKNC QGNLQFADE VELSLVNSA 
      YTYLADLRR AVFPFMKQK KNKFLPVFL LEGRRGSGK 
      SLLVKCLAS TQGMHLYTI SNFDVTATT YAQNETKLR 
      NSFFAAKMA APSILQIKN FENFGKNNE GQYDERLIS 
      YFTTEVKTL FESNSFPLI LICCSSDKN IPIELKRTF 
      LKTFEIKAP NDQEREKIL NWILKSQDV TTDIDMSEI 
      ANKTHGFLF EDLQTLVHY AMTDFTNEK KSAERCVVS 
      QDYFFRALD LMQSNYSES LGAPRVPQV KWSDVGGLT 
      EVKEEIIKT IKLPLKHSE LLKTTGLKR SGILLYGPP 
      GTGKTLIAK AVATECGLC FLSVKGPEL LNMYVGQSE 
      QNVREVFEK ARDASPCII FFDELDSLA PNRGASGDS 
      GGVMDRVVS QLLAEMDGL NQTGTVFII GATNRPDLI 
      DPALLRPGR FDKLLYVGP CIDRDSKIA VLTALTRKF 
      TLENDSLIA EAVDLCPEN FSGADFYGV CSSAWMAAV 
      RRFVKTLEE GKNDRNSAT ASDVIVTLD DFKLAIKTI 
      KPSIRQEDL EYYNKLKSD FK
    CDS sequence
    • >TC002647 RA
      atgagtaag caaaaagac ctccggttt ttggtgtat 
      ttggccaaa attgcctac ccaagacac aattcgtac 
      tgttttatc atctacatg attgtaaac tactgtgat 
      attttgaag caacagcaa atatttacg ttgcgaggt 
      gttagtagc gaatttttg aacaacttt gttggtggg 
      actgttttg gacattgat aacacgata gttgcgaat 
      gataacgtt gaacaaact tgtattaaa tacgttgtc 
      gttaaagac accacgagg aaggttaat ttaattacg 
      aaaaaattt gacaccaaa tttgtagcg ttgttgagt 
      tacaatttg ttgtataac ttgaagggg gctataact 
      tgcagtttt aagaactgt cagggaaac ctccaattt 
      gccgatgaa gtcgagttg tctttggtc aattcggct 
      tacacgtat ttggcggac ttacggaga gctgttttt 
      ccgtttatg aagcaaaag aaaaataaa tttttgccg 
      gtttttcta ctcgaaggg cgcagaggt agtggcaaa 
      agtttgttg gttaaatgt ttggcttcc acgcaaggg 
      atgcatttg tacacaatc agtaatttt gacgttact 
      gcgaccact tacgcccaa aatgagacg aaattgcga 
      aatagtttt tttgctgcg aaaatggcc gccccctcc 
      atcttgcaa ataaagaat tttgagaat tttgggaaa 
      aataacgaa ggacaatat gacgaacgt ctgattagt 
      tattttaca actgaagtc aaaactctc ttcgaaagc 
      aattcattt ccgttgatt ctaatctgc tgtagcagc 
      gataaaaat attccgatc gaactcaaa cgaactttt 
      ctgaaaact ttcgaaata aaagcacca aacgaccaa 
      gaaagagaa aaaatatta aactggatt ttaaaaagc 
      caagatgta acgactgat attgacatg agcgagatt 
      gccaacaaa acgcacggt tttttgttc gaggacttg 
      caaaccttg gttcattat gctatgaca gatttcact 
      aacgaaaaa aaatcagcg gagagatgt gttgtgtct 
      caggattat tttttccgg gctttggac ttgatgcaa 
      tcgaattat agcgagagt ttgggggcg ccaagagtg 
      cctcaagtc aagtggtca gacgttggc ggtttaacc 
      gaagtgaag gaagaaata attaaaacg ataaaattg 
      ccgctgaaa cactccgag cttttgaaa acgaccgga 
      ttgaagcga tccgggatt ttgctttac ggccctccg 
      ggcaccgga aaaactcta atcgccaaa gcggtcgcc 
      accgaatgc ggtttatgt tttttgtca gtcaaaggc 
      cccgaattg ttgaatatg tatgtgggc cagtcggag 
      caaaacgtg cgcgaggtg tttgagaag gcgcgtgac 
      gcctccccg tgcataatt tttttcgac gaactggac 
      tctctggcc cccaatcgg ggcgcttcc ggtgattcc 
      ggcggcgtg atggacagg gtggtttcg cagcttttg 
      gcggaaatg gacggtttg aatcaaacc ggcactgtt 
      tttatcata ggagcaaca aataggcca gacttgatc 
      gatccggca cttctaaga ccgggaaga tttgataag 
      ttgctttat gtgggaccg tgcatcgat cgcgattcg 
      aaaattgcc gttctgact gccttaaca cgaaagttt 
      acgctggaa aatgacagt ttgatagcg gaagcggtt 
      gatttgtgc ccggaaaat ttcagtggc gccgatttt 
      tatggcgtt tgctcgagt gcatggatg gctgcagta 
      aggcgattt gtgaagact ttggaggaa gggaaaaat 
      gacagaaat agcgccaca gcaagcgac gtaatcgtg 
      actctcgac gactttaag ctcgctatc aagaccatc 
      aagccatca attaggcaa gaagacttg gaatactat 
      aataaactg aagtccgat tttaaataa
    mRNA sequence
    • >TC002647 RA
      agagggttt taagttgat aaataacgc acaaagtgg 
      cgttagggg tgccctcac attttttaa aatattcta 
      gttattttg tccccgcta ggttgtgca cgtcaagtt 
      tgacagcga ttctaaccc aacttttgc aactccaca 
      ggtgttaac aaagtgttc taataataa acaattatg 
      acttaattg tggtataaa gtgaccagt tttgttaaa 
      tttaactta agtgagata aaaaatgca actgacatt 
      gtgttggca agttgagta gtccaaaat gagtaagca 
      aaaagacct ccggttttt ggtgtattt ggccaaaat 
      tgcctaccc aagacacaa ttcgtactg ttttatcat 
      ctacatgat tgtaaacta ctgtgatat tttgaagca 
      acagcaaat atttacgtt gcgaggtgt tagtagcga 
      atttttgaa caactttgt tggtgggac tgttttgga 
      cattgataa cacgatagt tgcgaatga taacgttga 
      acaaacttg tattaaata cgttgtcgt taaagacac 
      cacgaggaa ggttaattt aattacgaa aaaatttga 
      caccaaatt tgtagcgtt gttgagtta caatttgtt 
      gtataactt gaagggggc tataacttg cagttttaa 
      gaactgtca gggaaacct ccaatttgc cgatgaagt 
      cgagttgtc tttggtcaa ttcggctta cacgtattt 
      ggcggactt acggagagc tgtttttcc gtttatgaa 
      gcaaaagaa aaataaatt tttgccggt ttttctact 
      cgaagggcg cagaggtag tggcaaaag tttgttggt 
      taaatgttt ggcttccac gcaagggat gcatttgta 
      cacaatcag taattttga cgttactgc gaccactta 
      cgcccaaaa tgagacgaa attgcgaaa tagtttttt 
      tgctgcgaa aatggccgc cccctccat cttgcaaat 
      aaagaattt tgagaattt tgggaaaaa taacgaagg 
      acaatatga cgaacgtct gattagtta ttttacaac 
      tgaagtcaa aactctctt cgaaagcaa ttcatttcc 
      gttgattct aatctgctg tagcagcga taaaaatat 
      tccgatcga actcaaacg aacttttct gaaaacttt 
      cgaaataaa agcaccaaa cgaccaaga aagagaaaa 
      aatattaaa ctggatttt aaaaagcca agatgtaac 
      gactgatat tgacatgag cgagattgc caacaaaac 
      gcacggttt tttgttcga ggacttgca aaccttggt 
      tcattatgc tatgacaga tttcactaa cgaaaaaaa 
      atcagcgga gagatgtgt tgtgtctca ggattattt 
      tttccgggc tttggactt gatgcaatc gaattatag 
      cgagagttt gggggcgcc aagagtgcc tcaagtcaa 
      gtggtcaga cgttggcgg tttaaccga agtgaagga 
      agaaataat taaaacgat aaaattgcc gctgaaaca 
      ctccgagct tttgaaaac gaccggatt gaagcgatc 
      cgggatttt gctttacgg ccctccggg caccggaaa 
      aactctaat cgccaaagc ggtcgccac cgaatgcgg 
      tttatgttt tttgtcagt caaaggccc cgaattgtt 
      gaatatgta tgtgggcca gtcggagca aaacgtgcg 
      cgaggtgtt tgagaaggc gcgtgacgc ctccccgtg 
      cataatttt tttcgacga actggactc tctggcccc 
      caatcgggg cgcttccgg tgattccgg cggcgtgat 
      ggacagggt ggtttcgca gcttttggc ggaaatgga 
      cggtttgaa tcaaaccgg cactgtttt tatcatagg 
      agcaacaaa taggccaga cttgatcga tccggcact 
      tctaagacc gggaagatt tgataagtt gctttatgt 
      gggaccgtg catcgatcg cgattcgaa aattgccgt 
      tctgactgc cttaacacg aaagtttac gctggaaaa 
      tgacagttt gatagcgga agcggttga tttgtgccc 
      ggaaaattt cagtggcgc cgattttta tggcgtttg 
      ctcgagtgc atggatggc tgcagtaag gcgatttgt 
      gaagacttt ggaggaagg gaaaaatga cagaaatag 
      cgccacagc aagcgacgt aatcgtgac tctcgacga 
      ctttaagct cgctatcaa gaccatcaa gccatcaat 
      taggcaaga agacttgga atactataa taaactgaa 
      gtccgattt taaataaaa attgtgtca caaccttgg 
      ccgcacatt gtgcacaca gtaaataga aaataaaag 
      tgcaaacag caaaggtca cggctggtg gttggagca 
      gtttgttct acaatcaat taattgttg gtttttatg 
      taaaaaatg gaatgtgtc tgggttaat aaatgatga 
      gttggtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY