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Gene information
General information
TC002636 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 991609..1082966
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002636
    iBeetle screen iB_00418
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: animals lost, remarks: later than schedule, hours 24
    Pupal morphology
    remarks: animals lost, remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002636 RA
      MELKTVAFI FILLYTVHG KPTDSTQDS YDYLQNDNY 
      EDENEQPAE YEEEEKDDV NEENNKPLV ITTLGQEFT 
      ANLSATIVL PCTIVGKNF VRIWRKKRV TDQNSEKKG 
      PILFQSGMT VAPSPNMKL LEDGSLEIS NIHPQDEGV 
      YECAAMDTA NNSPKIYHK VTVTVQPTI DFLMTKNNR 
      TSFKSGDTL ELTCKAHGH PKPVISWHK GSKRFEVEG 
      ETLTINNIK HHDAGTYKC LADNKIGEP AFSHINIKV 
      DFKPVITVE RISVSSENN SETELQCLV HSEPKAAVV 
      WIKDNGNIV SSPRVQIVS KDHEYNLVL KDLRDSDFG 
      VYTCSATNY LGNTKKHIK LVKTPAIVR ITKSRTEAN 
      EVVLTWEVQ SKSNLSDHE FVYRKKGEQ KWKRIYPEV 
      APMGDEHYS VKYTFEKNL DPGDYETKM RSANAYGWS 
      EYSDVVDFQ KVHNKHGHH KDQKKGHKH GKERQITTP 
      TPSTEPAEH QESEIKSGS VKDSSKSVT ESSASSVTP 
      TALMTSIAL ITFLQSLRI
    • >TC002636 RB
      MELKTVAFI FILLYTVHG KPTDSTQDS YDYLQNDNY 
      EDENEQPAE YEEEEKDDV NEENNKPLV ITTLGQEFT 
      ANLSATIVL PCTIVGKNF VRIWRKKRV TDQNSEKKG 
      PILFQSGMT VAPSPNMKL LEDGSLEIS NIHPQDEGV 
      YECAAMDTA NNSPKIYHK VTVTVQPTI DFLMTKNNR 
      TSFKSGDTL ELTCKAHGH PKPVISWHK GSKRFEVEG 
      ETLTINNIK HHDAGTYKC LADNKIGEP AFSHINIKV 
      DFKPVITVE RISVSSENN SETELQCLV HSEPKAAVV 
      WIKDNGNIV SSPRVQIVS KDHEYNLVL KDLRDSDFG 
      VYTCSATNY LGNTKKHIK LVKTPAIVR ITKSRTEAN 
      EVVLTWEVQ SKSNLSDHE FVYRKKGEQ KWKRIYPEV 
      APMGDEHYS VKYTFEKNL DPGDYETKM RSANAYGWS 
      EYSDVVDFQ KVTESSASS VTPTALMTS IALITFLQS 
      LRI
    • >TC002636 RC
      MELKTVAFI FILLYTVHG KPTDSTQDS YDYLQNDNY 
      EDENEQPAE YEEEEKDDV NEENNKPLV ITTLGQEFT 
      ANLSATIVL PCTIVGKNF VRIWRKKRV TDQNSEKKG 
      PILFQSGMT VAPSPNMKL LEDGSLEIS NIHPQDEGV 
      YECAAMDTA NNSPKIYHK VTVTVQPTI DFLMTKNNR 
      TSFKSGDTL ELTCKAHGH PKPVISWHK GSKRFEVEG 
      ETLTINNIK HHDAGTYKC LADNKIGEP AFSHINIKV 
      DFKPVITVE RISVSSENN SETELQCLV HSEPKAAVV 
      WIKDNGNIV SSPRVQIVS KDHEYNLVL KDLRDSDFG 
      VYTCSATNY LGNTKKHIK LVKTPAIVR ITKSRTEAN 
      EVVLTWEVQ SKSNLSDHE FVYRKKGEQ KWKRIYPEV 
      APMGDEHYS VKYTFEKNL DPGDYETKM RSANAYGWS 
      EYSDVVDFQ KVHNKHGHH KDQKKGHKH GKVTESSAS 
      SVTPTALMT SIALITFLQ SLRI
    CDS sequence
    • >TC002636 RA
      atggaactt aaaacggtg gcatttatt tttatactt 
      ctatatacc gtacacggc aaacccaca gattctact 
      caggactcc tacgattac ctccagaac gacaactac 
      gaggacgaa aatgagcaa ccggcagaa tacgaagaa 
      gaagagaaa gacgatgtt aatgaagaa aacaataag 
      ccactagta atcaccacc ttggggcaa gagtttacg 
      gctaatttg agtgccacg attgtctta ccgtgtacc 
      attgttgga aaaaatttc gtccgaatt tggcgaaag 
      aagagagta accgaccaa aattcggaa aagaaggga 
      cccatcctg tttcaatct ggaatgaca gtcgcacct 
      tcgcccaac atgaaactc ctcgaagac ggttcgctt 
      gaaatatca aacatccac ccccaagac gagggtgtt 
      tacgaatgt gccgccatg gacacggcc aacaacagc 
      cccaaaatc taccacaaa gttacagtg accgttcaa 
      cccactatc gattttctt atgaccaaa aataacaga 
      actagtttc aaaagcgga gacactctg gaattgacc 
      tgcaaagcc cacgggcac cccaaacct gtgatttcg 
      tggcacaaa ggctccaaa aggttcgaa gtcgaaggt 
      gaaaccttg acgatcaac aacatcaag catcacgac 
      gctggaact tacaaatgc ttggctgat aacaaaatc 
      ggagagcca gccttcagt cacattaat attaaagtc 
      gatttcaag ccggtcata accgtcgag agaattagc 
      gtaagttcc gaaaacaac tctgagact gaattacag 
      tgcctggtt cactcggaa cccaaagct gccgtagtc 
      tggatcaaa gacaacggg aatatcgtt agcagcccc 
      cgagtccaa atagtttcc aaagaccac gaatataac 
      ctagttttg aaagacttg cgcgactct gatttcggt 
      gtttacact tgttcagcg accaattat ctcggaaac 
      acgaaaaaa catatcaaa ctggtcaaa actccggca 
      attgtccgc attaccaag tcgaggacg gaagccaac 
      gaagttgtt ttgacttgg gaggtgcag tcgaaatct 
      aatctgtcc gaccatgag ttcgtgtac aggaaaaaa 
      ggggagcaa aaatggaag aggatttat ccagaagtg 
      gctccaatg ggcgatgag cattattcc gtgaaatat 
      actttcgag aaaaatctc gatcctggg gattatgag 
      accaagatg cggtcggca aatgcttac ggttggtcg 
      gagtattcc gacgttgtc gacttccag aaagttcat 
      aataaacac ggacaccac aaagatcag aaaaaaggt 
      cataaacac ggaaaagaa agacaaata actacaccc 
      acaccttcc acagaaccc gcagaacac caagaaagc 
      gaaattaaa agtggatct gttaaagac tcgtcgaaa 
      tcagttact gagtcatcg gcttcttcc gttactcca 
      actgcgcta atgactagc atagcctta attaccttc 
      ctacaatca ctgaggata taa
    • >TC002636 RB
      atggaactt aaaacggtg gcatttatt tttatactt 
      ctatatacc gtacacggc aaacccaca gattctact 
      caggactcc tacgattac ctccagaac gacaactac 
      gaggacgaa aatgagcaa ccggcagaa tacgaagaa 
      gaagagaaa gacgatgtt aatgaagaa aacaataag 
      ccactagta atcaccacc ttggggcaa gagtttacg 
      gctaatttg agtgccacg attgtctta ccgtgtacc 
      attgttgga aaaaatttc gtccgaatt tggcgaaag 
      aagagagta accgaccaa aattcggaa aagaaggga 
      cccatcctg tttcaatct ggaatgaca gtcgcacct 
      tcgcccaac atgaaactc ctcgaagac ggttcgctt 
      gaaatatca aacatccac ccccaagac gagggtgtt 
      tacgaatgt gccgccatg gacacggcc aacaacagc 
      cccaaaatc taccacaaa gttacagtg accgttcaa 
      cccactatc gattttctt atgaccaaa aataacaga 
      actagtttc aaaagcgga gacactctg gaattgacc 
      tgcaaagcc cacgggcac cccaaacct gtgatttcg 
      tggcacaaa ggctccaaa aggttcgaa gtcgaaggt 
      gaaaccttg acgatcaac aacatcaag catcacgac 
      gctggaact tacaaatgc ttggctgat aacaaaatc 
      ggagagcca gccttcagt cacattaat attaaagtc 
      gatttcaag ccggtcata accgtcgag agaattagc 
      gtaagttcc gaaaacaac tctgagact gaattacag 
      tgcctggtt cactcggaa cccaaagct gccgtagtc 
      tggatcaaa gacaacggg aatatcgtt agcagcccc 
      cgagtccaa atagtttcc aaagaccac gaatataac 
      ctagttttg aaagacttg cgcgactct gatttcggt 
      gtttacact tgttcagcg accaattat ctcggaaac 
      acgaaaaaa catatcaaa ctggtcaaa actccggca 
      attgtccgc attaccaag tcgaggacg gaagccaac 
      gaagttgtt ttgacttgg gaggtgcag tcgaaatct 
      aatctgtcc gaccatgag ttcgtgtac aggaaaaaa 
      ggggagcaa aaatggaag aggatttat ccagaagtg 
      gctccaatg ggcgatgag cattattcc gtgaaatat 
      actttcgag aaaaatctc gatcctggg gattatgag 
      accaagatg cggtcggca aatgcttac ggttggtcg 
      gagtattcc gacgttgtc gacttccag aaagttact 
      gagtcatcg gcttcttcc gttactcca actgcgcta 
      atgactagc atagcctta attaccttc ctacaatca 
      ctgaggata taa
    • >TC002636 RC
      atggaactt aaaacggtg gcatttatt tttatactt 
      ctatatacc gtacacggc aaacccaca gattctact 
      caggactcc tacgattac ctccagaac gacaactac 
      gaggacgaa aatgagcaa ccggcagaa tacgaagaa 
      gaagagaaa gacgatgtt aatgaagaa aacaataag 
      ccactagta atcaccacc ttggggcaa gagtttacg 
      gctaatttg agtgccacg attgtctta ccgtgtacc 
      attgttgga aaaaatttc gtccgaatt tggcgaaag 
      aagagagta accgaccaa aattcggaa aagaaggga 
      cccatcctg tttcaatct ggaatgaca gtcgcacct 
      tcgcccaac atgaaactc ctcgaagac ggttcgctt 
      gaaatatca aacatccac ccccaagac gagggtgtt 
      tacgaatgt gccgccatg gacacggcc aacaacagc 
      cccaaaatc taccacaaa gttacagtg accgttcaa 
      cccactatc gattttctt atgaccaaa aataacaga 
      actagtttc aaaagcgga gacactctg gaattgacc 
      tgcaaagcc cacgggcac cccaaacct gtgatttcg 
      tggcacaaa ggctccaaa aggttcgaa gtcgaaggt 
      gaaaccttg acgatcaac aacatcaag catcacgac 
      gctggaact tacaaatgc ttggctgat aacaaaatc 
      ggagagcca gccttcagt cacattaat attaaagtc 
      gatttcaag ccggtcata accgtcgag agaattagc 
      gtaagttcc gaaaacaac tctgagact gaattacag 
      tgcctggtt cactcggaa cccaaagct gccgtagtc 
      tggatcaaa gacaacggg aatatcgtt agcagcccc 
      cgagtccaa atagtttcc aaagaccac gaatataac 
      ctagttttg aaagacttg cgcgactct gatttcggt 
      gtttacact tgttcagcg accaattat ctcggaaac 
      acgaaaaaa catatcaaa ctggtcaaa actccggca 
      attgtccgc attaccaag tcgaggacg gaagccaac 
      gaagttgtt ttgacttgg gaggtgcag tcgaaatct 
      aatctgtcc gaccatgag ttcgtgtac aggaaaaaa 
      ggggagcaa aaatggaag aggatttat ccagaagtg 
      gctccaatg ggcgatgag cattattcc gtgaaatat 
      actttcgag aaaaatctc gatcctggg gattatgag 
      accaagatg cggtcggca aatgcttac ggttggtcg 
      gagtattcc gacgttgtc gacttccag aaagttcat 
      aataaacac ggacaccac aaagatcag aaaaaaggt 
      cataaacac ggaaaagtt actgagtca tcggcttct 
      tccgttact ccaactgcg ctaatgact agcatagcc 
      ttaattacc ttcctacaa tcactgagg atataa
    mRNA sequence
    • >TC002636 RA
      ctcaccaca catccaaca acgaagaaa aagcgataa 
      caagtttgc ataaataaa aagctaaaa gaaacggaa 
      agctcattc cattctcga tcttgcctc gaaagcggc 
      ggcagcagc agagttgct ttgcattcg gagccgaag 
      gcatagcga aggtttcaa ctcgtcttg tccatgcgc 
      aagcgtatc ttttaaaaa tacattcgg cttttgtcc 
      cgcgaatgt tgcggactt cgggcgcgc tgtgtttgt 
      gctccggtg aagtggaat gaaataaaa aaaattaac 
      ttggactaa gtgttttgt tactcgagg aaaaccaat 
      ttttagcat cactactac taactagca gaaaatgga 
      acttaaaac ggtggcatt tatttttat acttctata 
      taccgtaca cggcaaacc cacagattc tactcagga 
      ctcctacga ttacctcca gaacgacaa ctacgagga 
      cgaaaatga gcaaccggc agaatacga agaagaaga 
      gaaagacga tgttaatga agaaaacaa taagccact 
      agtaatcac caccttggg gcaagagtt tacggctaa 
      tttgagtgc cacgattgt cttaccgtg taccattgt 
      tggaaaaaa tttcgtccg aatttggcg aaagaagag 
      agtaaccga ccaaaattc ggaaaagaa gggacccat 
      cctgtttca atctggaat gacagtcgc accttcgcc 
      caacatgaa actcctcga agacggttc gcttgaaat 
      atcaaacat ccaccccca agacgaggg tgtttacga 
      atgtgccgc catggacac ggccaacaa cagccccaa 
      aatctacca caaagttac agtgaccgt tcaacccac 
      tatcgattt tcttatgac caaaaataa cagaactag 
      tttcaaaag cggagacac tctggaatt gacctgcaa 
      agcccacgg gcaccccaa acctgtgat ttcgtggca 
      caaaggctc caaaaggtt cgaagtcga aggtgaaac 
      cttgacgat caacaacat caagcatca cgacgctgg 
      aacttacaa atgcttggc tgataacaa aatcggaga 
      gccagcctt cagtcacat taatattaa agtcgattt 
      caagccggt cataaccgt cgagagaat tagcgtaag 
      ttccgaaaa caactctga gactgaatt acagtgcct 
      ggttcactc ggaacccaa agctgccgt agtctggat 
      caaagacaa cgggaatat cgttagcag cccccgagt 
      ccaaatagt ttccaaaga ccacgaata taacctagt 
      tttgaaaga cttgcgcga ctctgattt cggtgttta 
      cacttgttc agcgaccaa ttatctcgg aaacacgaa 
      aaaacatat caaactggt caaaactcc ggcaattgt 
      ccgcattac caagtcgag gacggaagc caacgaagt 
      tgttttgac ttgggaggt gcagtcgaa atctaatct 
      gtccgacca tgagttcgt gtacaggaa aaaagggga 
      gcaaaaatg gaagaggat ttatccaga agtggctcc 
      aatgggcga tgagcatta ttccgtgaa atatacttt 
      cgagaaaaa tctcgatcc tggggatta tgagaccaa 
      gatgcggtc ggcaaatgc ttacggttg gtcggagta 
      ttccgacgt tgtcgactt ccagaaagt tcataataa 
      acacggaca ccacaaaga tcagaaaaa aggtcataa 
      acacggaaa agaaagaca aataactac acccacacc 
      ttccacaga acccgcaga acaccaaga aagcgaaat 
      taaaagtgg atctgttaa agactcgtc gaaatcagt 
      tactgagtc atcggcttc ttccgttac tccaactgc 
      gctaatgac tagcatagc cttaattac cttcctaca 
      atcactgag gatataata cacgagagc ttgtgtaat 
      ggaggaact tatcagcgc acttactgg tagcttaaa 
      aagttaaac caaagtatt taaagtaat tttaagtaa 
      attataaaa cagttgatt tattcttgt atacttacc 
      tttacttac tgtaaattc agtaactgt acatacgtt 
      ttgtacgat ttattgtgt tcattttag ttgtcttat 
      ctcatgagt gcttctttg caacgtatt tttttttgt 
      g
    • >TC002636 RB
      ctcaccaca catccaaca acgaagaaa aagcgataa 
      caagtttgc ataaataaa aagctaaaa gaaacggaa 
      agctcattc cattctcga tcttgcctc gaaagcggc 
      ggcagcagc agagttgct ttgcattcg gagccgaag 
      gcatagcga aggtttcaa ctcgtcttg tccatgcgc 
      aagcgtatc ttttaaaaa tacattcgg cttttgtcc 
      cgcgaatgt tgcggactt cgggcgcgc tgtgtttgt 
      gctccggtg aagtggaat gaaataaaa aaaattaac 
      ttggactaa gtgttttgt tactcgagg aaaaccaat 
      ttttagcat cactactac taactagca gaaaatgga 
      acttaaaac ggtggcatt tatttttat acttctata 
      taccgtaca cggcaaacc cacagattc tactcagga 
      ctcctacga ttacctcca gaacgacaa ctacgagga 
      cgaaaatga gcaaccggc agaatacga agaagaaga 
      gaaagacga tgttaatga agaaaacaa taagccact 
      agtaatcac caccttggg gcaagagtt tacggctaa 
      tttgagtgc cacgattgt cttaccgtg taccattgt 
      tggaaaaaa tttcgtccg aatttggcg aaagaagag 
      agtaaccga ccaaaattc ggaaaagaa gggacccat 
      cctgtttca atctggaat gacagtcgc accttcgcc 
      caacatgaa actcctcga agacggttc gcttgaaat 
      atcaaacat ccaccccca agacgaggg tgtttacga 
      atgtgccgc catggacac ggccaacaa cagccccaa 
      aatctacca caaagttac agtgaccgt tcaacccac 
      tatcgattt tcttatgac caaaaataa cagaactag 
      tttcaaaag cggagacac tctggaatt gacctgcaa 
      agcccacgg gcaccccaa acctgtgat ttcgtggca 
      caaaggctc caaaaggtt cgaagtcga aggtgaaac 
      cttgacgat caacaacat caagcatca cgacgctgg 
      aacttacaa atgcttggc tgataacaa aatcggaga 
      gccagcctt cagtcacat taatattaa agtcgattt 
      caagccggt cataaccgt cgagagaat tagcgtaag 
      ttccgaaaa caactctga gactgaatt acagtgcct 
      ggttcactc ggaacccaa agctgccgt agtctggat 
      caaagacaa cgggaatat cgttagcag cccccgagt 
      ccaaatagt ttccaaaga ccacgaata taacctagt 
      tttgaaaga cttgcgcga ctctgattt cggtgttta 
      cacttgttc agcgaccaa ttatctcgg aaacacgaa 
      aaaacatat caaactggt caaaactcc ggcaattgt 
      ccgcattac caagtcgag gacggaagc caacgaagt 
      tgttttgac ttgggaggt gcagtcgaa atctaatct 
      gtccgacca tgagttcgt gtacaggaa aaaagggga 
      gcaaaaatg gaagaggat ttatccaga agtggctcc 
      aatgggcga tgagcatta ttccgtgaa atatacttt 
      cgagaaaaa tctcgatcc tggggatta tgagaccaa 
      gatgcggtc ggcaaatgc ttacggttg gtcggagta 
      ttccgacgt tgtcgactt ccagaaagt tactgagtc 
      atcggcttc ttccgttac tccaactgc gctaatgac 
      tagcatagc cttaattac cttcctaca atcactgag 
      gatataata cacgagagc ttgtgtaat ggaggaact 
      tatcagcgc acttactgg tagcttaaa aagttaaac 
      caaagtatt taaagtaat tttaagtaa attataaaa 
      cagttgatt tattcttgt atacttacc tttacttac 
      tgtaaattc agtaactgt acatacgtt ttgtacgat 
      ttattgtgt tcattttag ttgtcttat ctcatgagt 
      gcttctttg caacgtatt tttttttgt g
    • >TC002636 RC
      ctcaccaca catccaaca acgaagaaa aagcgataa 
      caagtttgc ataaataaa aagctaaaa gaaacggaa 
      agctcattc cattctcga tcttgcctc gaaagcggc 
      ggcagcagc agagttgct ttgcattcg gagccgaag 
      gcatagcga aggtttcaa ctcgtcttg tccatgcgc 
      aagcgtatc ttttaaaaa tacattcgg cttttgtcc 
      cgcgaatgt tgcggactt cgggcgcgc tgtgtttgt 
      gctccggtg aagtggaat gaaataaaa aaaattaac 
      ttggactaa gtgttttgt tactcgagg aaaaccaat 
      ttttagcat cactactac taactagca gaaaatgga 
      acttaaaac ggtggcatt tatttttat acttctata 
      taccgtaca cggcaaacc cacagattc tactcagga 
      ctcctacga ttacctcca gaacgacaa ctacgagga 
      cgaaaatga gcaaccggc agaatacga agaagaaga 
      gaaagacga tgttaatga agaaaacaa taagccact 
      agtaatcac caccttggg gcaagagtt tacggctaa 
      tttgagtgc cacgattgt cttaccgtg taccattgt 
      tggaaaaaa tttcgtccg aatttggcg aaagaagag 
      agtaaccga ccaaaattc ggaaaagaa gggacccat 
      cctgtttca atctggaat gacagtcgc accttcgcc 
      caacatgaa actcctcga agacggttc gcttgaaat 
      atcaaacat ccaccccca agacgaggg tgtttacga 
      atgtgccgc catggacac ggccaacaa cagccccaa 
      aatctacca caaagttac agtgaccgt tcaacccac 
      tatcgattt tcttatgac caaaaataa cagaactag 
      tttcaaaag cggagacac tctggaatt gacctgcaa 
      agcccacgg gcaccccaa acctgtgat ttcgtggca 
      caaaggctc caaaaggtt cgaagtcga aggtgaaac 
      cttgacgat caacaacat caagcatca cgacgctgg 
      aacttacaa atgcttggc tgataacaa aatcggaga 
      gccagcctt cagtcacat taatattaa agtcgattt 
      caagccggt cataaccgt cgagagaat tagcgtaag 
      ttccgaaaa caactctga gactgaatt acagtgcct 
      ggttcactc ggaacccaa agctgccgt agtctggat 
      caaagacaa cgggaatat cgttagcag cccccgagt 
      ccaaatagt ttccaaaga ccacgaata taacctagt 
      tttgaaaga cttgcgcga ctctgattt cggtgttta 
      cacttgttc agcgaccaa ttatctcgg aaacacgaa 
      aaaacatat caaactggt caaaactcc ggcaattgt 
      ccgcattac caagtcgag gacggaagc caacgaagt 
      tgttttgac ttgggaggt gcagtcgaa atctaatct 
      gtccgacca tgagttcgt gtacaggaa aaaagggga 
      gcaaaaatg gaagaggat ttatccaga agtggctcc 
      aatgggcga tgagcatta ttccgtgaa atatacttt 
      cgagaaaaa tctcgatcc tggggatta tgagaccaa 
      gatgcggtc ggcaaatgc ttacggttg gtcggagta 
      ttccgacgt tgtcgactt ccagaaagt tcataataa 
      acacggaca ccacaaaga tcagaaaaa aggtcataa 
      acacggaaa agttactga gtcatcggc ttcttccgt 
      tactccaac tgcgctaat gactagcat agccttaat 
      taccttcct acaatcact gaggatata atacacgag 
      agcttgtgt aatggagga acttatcag cgcacttac 
      tggtagctt aaaaagtta aaccaaagt atttaaagt 
      aattttaag taaattata aaacagttg atttattct 
      tgtatactt acctttact tactgtaaa ttcagtaac 
      tgtacatac gttttgtac gatttattg tgttcattt 
      tagttgtct tatctcatg agtgcttct ttgcaacgt 
      atttttttt tgtg