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Gene information
General information
TC002629 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 1273448..1290202
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002629
      iBeetle screen iB_00417
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002629 RA
        MDEELTNLQ LDKPGAWGN KTNEQLAKH TIDSQFESK 
        PIDRSLHLL RKGDDIQKL SVIQALPQI LQYDPNGTL 
        TRLVPKIQQ ELPKSSCEF HIATSKIFK LLIEKQLPL 
        NLTGAVIQG TESKDPVVS NAWTETLLA VIPTLPIDQ 
        IKSAVIPLA LAKSQPSSP VYCRVSSCK ILGKCASHP 
        KMQSIDVKA SILPAVQSL CQDCFQDVR ATMCSQLHF 
        IAQVYANKM KIILIFRQD VLCREQCAF NLPAVTQFT 
        VARIPNQLE LWHTIFSGL ANDSCYIVR KTTAACMHD 
        IAKILGPQC KIIKDDLIK LLRDDAEEV LQQLVPHVG 
        PTLELLCAN GVLSREANT PACLEIARG LLKCQNELA 
        KSYNWRIKS NFLQQLERL PFCMTSDFI HQHFSPVIL 
        SCVLEARPK PVRSQASRT LLIFLRYNL KEVQRKWIR 
        DNLVTHLCY AKSCYTRHV FILACVHAA ELFSNNYFK 
        KNFFEPLLS LADDPISNI RLCVIGFLP MLYKMLVMP 
        EDRKLQTNI DNIFSKLDM TEKDKDVKD ILKTKLKEI 
        RNIGPSNKH DYLVEQRRK EEEENKILQ GKMVVSTAA 
        VGNNVTAAP KTAPSIASR DVPSTTTTR SQPITSRQS 
        VSSPRVSKS PSTISKSPS AGSGSNTAA SDMSFLEQH 
        FYIDAGVSL PRIEQPIDM EIRKVVTDF DQQLNLYSE 
        DPNVIPTVS VENVNIEAM SESELKKLE TSTTNITDD 
        VKVNLRKRS KKSTNRKSL LSPPKDICL KYKRYSSVF 
        PADSSTSKT TLNRRSLNI GAGDLSKIP VCVKKSHSN 
        ADIEHVFEC GDGDKANRT SGKVKHKPN EATQSKIRP 
        KSEILTRLD KQKDDSAIT SSISKNNVP ISSINKGKT 
        SGLPVLKKK TQSEIKSK
      • >TC002629 RB
        MDLGLYDYP PQTSVDDDY FLLQELLMK GDDIQKLSV 
        IQALPQILQ YDPNGTLTR LVPKIQQEL PKSSCEFHI 
        ATSKIFKLL IEKQLPLNL TGAVIQGTE SKDPVVSNA 
        WTETLLAVI PTLPIDQIK SAVIPLALA KSQPSSPVY 
        CRVSSCKIL GKCASHPKM QSIDVKASI LPAVQSLCQ 
        DCFQDVRAT MCSQLHFIA QVYANKMKI ILIFRQDVL 
        CREQCAFNL PAVTQFTVA RIPNQLELW HTIFSGLAN 
        DSCYIVRKT TAACMHDIA KILGPQCKI IKDDLIKLL 
        RDDAEEVLQ QLVPHVGPT LELLCANGV LSREANTPA 
        CLEIARGLL KCQNELAKS YNWRIKSNF LQQLERLPF 
        CMTSDFIHQ HFSPVILSC VLEARPKPV RSQASRTLL 
        IFLRYNLKE VQRKWIRDN LVTHLCYAK SCYTRHVFI 
        LACVHAAEL FSNNYFKKN FFEPLLSLA DDPISNIRL 
        CVIGFLPML YKMLVMPED RKLQTNIDN IFSKLDMTE 
        KDKDVKDIL KTKLKEIRN IGPSNKHDY LVEQRRKEE 
        EENKILQGK MVVSTAAVG NNVTAAPKT APSIASRDV 
        PSTTTTRSQ PITSRQSVS SPRVSKSPS TISKSPSAG 
        SGSNTAASD MSFLEQHFY IDAGVSLPR IEQPIDMEI 
        RKVVTDFDQ QLNLYSEDP NVIPTVSVE NVNIEAMSE 
        SELKKLETS TTNITDDVK VNLRKRSKK STNRKSLLS 
        PPKDICLKY KRYSSVFPA DSSTSKTTL NRRSLNIGA 
        GDLSKIPVC VKKSHSNAD IEHVFECGD GDKANRTSG 
        KVKHKPNEA TQSKIRPKS EILTRLDKQ KDDSAITSS 
        ISKNNVPIS SINKGKTSG LPVLKKKTQ SEIKSK
      CDS sequence
      • >TC002629 RA
        atggatgaa gaactcacc aatctgcag ctggacaaa 
        ccaggggct tggggaaac aaaacgaac gaacaactc 
        gcaaaacac acaattgac tcgcagttt gaatcaaaa 
        ccgattgac agaagtctt catttgctg cggaaaggc 
        gatgacatt cagaaatta agcgtaatt caggctttg 
        ccccaaatc ttgcaatat gatccaaac ggaacactt 
        acaagactt gtgccaaaa atacaacaa gaactgcca 
        aaatcctca tgcgagttt cacatagcc actagtaag 
        atatttaaa ttgttgatt gaaaagcaa ctgcctttg 
        aatttaacg ggggctgta atacaagga accgagtct 
        aaagaccct gtggtttcg aatgcgtgg actgagact 
        cttttggct gttattcca actctgcca attgatcaa 
        attaaatca gcagtgata ccgttagca ttggctaaa 
        tcgcagccc tcaagtcct gtgtattgt cgcgtttct 
        agctgtaaa atcttggga aaatgtgcg tcacatcca 
        aaaatgcag tcgatagac gtcaaagct tcgattttg 
        ccggctgtt caaagtttg tgccaagat tgttttcaa 
        gacgtgagg gccacaatg tgttcgcag ttgcacttc 
        attgcacag gtgtacgca aataaaatg aaaataatt 
        ctaattttt cgacaggac gtcttatgc agagaacaa 
        tgcgctttc aatttaccc gcagtcaca caattcaca 
        gtggctcgt ataccaaac cagttggag ctctggcat 
        accatcttc agtggtcta gcaaacgat tcctgttac 
        atcgttagg aaaaccaca gctgcttgt atgcacgat 
        atagccaaa attttgggt ccccaatgt aaaataatt 
        aaagacgat ttaattaaa ttattacgt gacgatgct 
        gaagaagta ctacaacaa ttagtacct catgtgggc 
        ccaactttg gaattactt tgcgccaat ggtgtgctt 
        tcgcgagaa gccaacact ccggcgtgt ctcgaaatc 
        gcaagaggg ttgttaaag tgccagaat gagttagca 
        aaatcttac aactggcgg attaaatcg aattttctg 
        caacagttg gaacgcctg cctttttgc atgacttcc 
        gacttcatc catcagcat ttcagtccg gttattttg 
        agttgtgtt ctagaagct aggcctaaa ccagtccgc 
        tcgcaggct agtcggact ttgctcatt tttcttcga 
        tacaatttg aaagaagtt caaagaaaa tggatccgt 
        gacaattta gtaacgcac ttgtgttat gcgaaatct 
        tgttacacc agacatgtt ttcatcctg gcctgtgtt 
        catgccgct gaacttttc agtaacaat tatttcaag 
        aagaatttt tttgaaccg ttattatca ttagctgat 
        gatccgatt tcaaatata cgtttgtgt gtcattggt 
        tttttacca atgttgtat aaaatgcta gtgatgcct 
        gaagataga aagcttcaa acgaacata gataatatt 
        ttttcgaag ttggacatg accgagaag gataaagat 
        gttaaagat attttgaag actaagctt aaggaaatt 
        agaaatatt ggaccttcg aacaaacat gattatttg 
        gtggaacag cggcggaaa gaagaagaa gagaataaa 
        atcctgcaa gggaaaatg gtcgtttcc acggcggct 
        gtaggaaat aatgtaaca gcagcgcca aaaacagca 
        ccttcgatc gcatccaga gatgttcca tctactaca 
        accactaga tctcaacct atcacttca aggcaatct 
        gtgtcatca cctcgtgtt agtaaatca ccgtcaact 
        atctctaaa tcaccttcg gcaggttcg ggttcaaat 
        acggctgca agcgatatg agcttctta gaacagcat 
        ttttacatt gatgctggc gtaagctta ccgcgtatc 
        gaacaacct atagatatg gaaatacgc aaagttgtg 
        acagatttc gatcaacaa ttgaattta tattcggaa 
        gatccgaac gtaataccg actgtctct gtcgaaaat 
        gttaacatt gaagcaatg tctgaaagt gaattgaaa 
        aaactggaa acatcaacc accaatatt acagacgat 
        gttaaagtg aacttgaga aagagaagt aagaaaagt 
        acaaatcgc aaatcactt ctaagtcca ccaaaagat 
        atctgcttg aaatacaaa agatattcg agtgtgttc 
        ccagccgat tcctcaaca tctaaaaca acattgaat 
        agaagaagc ctgaatata ggtgctggt gatttaagt 
        aaaattcca gtttgtgtg aaaaaatca cacagtaat 
        gctgatata gagcatgtt tttgaatgt ggagatggc 
        gataaagcg aatagaacc tcgggaaag gtgaaacat 
        aaaccgaat gaagccact caaagtaaa atcaggcct 
        aaatctgaa attttaaca cgtcttgac aaacaaaag 
        gacgatagt gcaataact tccagtatt agtaaaaat 
        aatgtgcca ataagtagt attaacaaa ggaaaaaca 
        tcaggattg ccagtgttg aaaaagaaa acacagtcc 
        gagatcaag agcaaataa
      • >TC002629 RB
        atggatttg gggttatac gattatccc ccgcagact 
        tcagtggac gatgattat tttttgtta caagagttg 
        ctcatgaaa ggcgatgac attcagaaa ttaagcgta 
        attcaggct ttgccccaa atcttgcaa tatgatcca 
        aacggaaca cttacaaga cttgtgcca aaaatacaa 
        caagaactg ccaaaatcc tcatgcgag tttcacata 
        gccactagt aagatattt aaattgttg attgaaaag 
        caactgcct ttgaattta acgggggct gtaatacaa 
        ggaaccgag tctaaagac cctgtggtt tcgaatgcg 
        tggactgag actcttttg gctgttatt ccaactctg 
        ccaattgat caaattaaa tcagcagtg ataccgtta 
        gcattggct aaatcgcag ccctcaagt cctgtgtat 
        tgtcgcgtt tctagctgt aaaatcttg ggaaaatgt 
        gcgtcacat ccaaaaatg cagtcgata gacgtcaaa 
        gcttcgatt ttgccggct gttcaaagt ttgtgccaa 
        gattgtttt caagacgtg agggccaca atgtgttcg 
        cagttgcac ttcattgca caggtgtac gcaaataaa 
        atgaaaata attctaatt tttcgacag gacgtctta 
        tgcagagaa caatgcgct ttcaattta cccgcagtc 
        acacaattc acagtggct cgtatacca aaccagttg 
        gagctctgg cataccatc ttcagtggt ctagcaaac 
        gattcctgt tacatcgtt aggaaaacc acagctgct 
        tgtatgcac gatatagcc aaaattttg ggtccccaa 
        tgtaaaata attaaagac gatttaatt aaattatta 
        cgtgacgat gctgaagaa gtactacaa caattagta 
        cctcatgtg ggcccaact ttggaatta ctttgcgcc 
        aatggtgtg ctttcgcga gaagccaac actccggcg 
        tgtctcgaa atcgcaaga gggttgtta aagtgccag 
        aatgagtta gcaaaatct tacaactgg cggattaaa 
        tcgaatttt ctgcaacag ttggaacgc ctgcctttt 
        tgcatgact tccgacttc atccatcag catttcagt 
        ccggttatt ttgagttgt gttctagaa gctaggcct 
        aaaccagtc cgctcgcag gctagtcgg actttgctc 
        atttttctt cgatacaat ttgaaagaa gttcaaaga 
        aaatggatc cgtgacaat ttagtaacg cacttgtgt 
        tatgcgaaa tcttgttac accagacat gttttcatc 
        ctggcctgt gttcatgcc gctgaactt ttcagtaac 
        aattatttc aagaagaat ttttttgaa ccgttatta 
        tcattagct gatgatccg atttcaaat atacgtttg 
        tgtgtcatt ggtttttta ccaatgttg tataaaatg 
        ctagtgatg cctgaagat agaaagctt caaacgaac 
        atagataat attttttcg aagttggac atgaccgag 
        aaggataaa gatgttaaa gatattttg aagactaag 
        cttaaggaa attagaaat attggacct tcgaacaaa 
        catgattat ttggtggaa cagcggcgg aaagaagaa 
        gaagagaat aaaatcctg caagggaaa atggtcgtt 
        tccacggcg gctgtagga aataatgta acagcagcg 
        ccaaaaaca gcaccttcg atcgcatcc agagatgtt 
        ccatctact acaaccact agatctcaa cctatcact 
        tcaaggcaa tctgtgtca tcacctcgt gttagtaaa 
        tcaccgtca actatctct aaatcacct tcggcaggt 
        tcgggttca aatacggct gcaagcgat atgagcttc 
        ttagaacag catttttac attgatgct ggcgtaagc 
        ttaccgcgt atcgaacaa cctatagat atggaaata 
        cgcaaagtt gtgacagat ttcgatcaa caattgaat 
        ttatattcg gaagatccg aacgtaata ccgactgtc 
        tctgtcgaa aatgttaac attgaagca atgtctgaa 
        agtgaattg aaaaaactg gaaacatca accaccaat 
        attacagac gatgttaaa gtgaacttg agaaagaga 
        agtaagaaa agtacaaat cgcaaatca cttctaagt 
        ccaccaaaa gatatctgc ttgaaatac aaaagatat 
        tcgagtgtg ttcccagcc gattcctca acatctaaa 
        acaacattg aatagaaga agcctgaat ataggtgct 
        ggtgattta agtaaaatt ccagtttgt gtgaaaaaa 
        tcacacagt aatgctgat atagagcat gtttttgaa 
        tgtggagat ggcgataaa gcgaataga acctcggga 
        aaggtgaaa cataaaccg aatgaagcc actcaaagt 
        aaaatcagg cctaaatct gaaatttta acacgtctt 
        gacaaacaa aaggacgat agtgcaata acttccagt 
        attagtaaa aataatgtg ccaataagt agtattaac 
        aaaggaaaa acatcagga ttgccagtg ttgaaaaag 
        aaaacacag tccgagatc aagagcaaa taa
      mRNA sequence
      • >TC002629 RA
        ggtcaaacc taaacaaat taacaaaaa tggatgaag 
        aactcacca atctgcagc tggacaaac caggggctt 
        ggggaaaca aaacgaacg aacaactcg caaaacaca 
        caattgact cgcagtttg aatcaaaac cgattgaca 
        gaagtcttc atttgctgc ggaaaggcg atgacattc 
        agaaattaa gcgtaattc aggctttgc cccaaatct 
        tgcaatatg atccaaacg gaacactta caagacttg 
        tgccaaaaa tacaacaag aactgccaa aatcctcat 
        gcgagtttc acatagcca ctagtaaga tatttaaat 
        tgttgattg aaaagcaac tgcctttga atttaacgg 
        gggctgtaa tacaaggaa ccgagtcta aagaccctg 
        tggtttcga atgcgtgga ctgagactc ttttggctg 
        ttattccaa ctctgccaa ttgatcaaa ttaaatcag 
        cagtgatac cgttagcat tggctaaat cgcagccct 
        caagtcctg tgtattgtc gcgtttcta gctgtaaaa 
        tcttgggaa aatgtgcgt cacatccaa aaatgcagt 
        cgatagacg tcaaagctt cgattttgc cggctgttc 
        aaagtttgt gccaagatt gttttcaag acgtgaggg 
        ccacaatgt gttcgcagt tgcacttca ttgcacagg 
        tgtacgcaa ataaaatga aaataattc taatttttc 
        gacaggacg tcttatgca gagaacaat gcgctttca 
        atttacccg cagtcacac aattcacag tggctcgta 
        taccaaacc agttggagc tctggcata ccatcttca 
        gtggtctag caaacgatt cctgttaca tcgttagga 
        aaaccacag ctgcttgta tgcacgata tagccaaaa 
        ttttgggtc cccaatgta aaataatta aagacgatt 
        taattaaat tattacgtg acgatgctg aagaagtac 
        tacaacaat tagtacctc atgtgggcc caactttgg 
        aattacttt gcgccaatg gtgtgcttt cgcgagaag 
        ccaacactc cggcgtgtc tcgaaatcg caagagggt 
        tgttaaagt gccagaatg agttagcaa aatcttaca 
        actggcgga ttaaatcga attttctgc aacagttgg 
        aacgcctgc ctttttgca tgacttccg acttcatcc 
        atcagcatt tcagtccgg ttattttga gttgtgttc 
        tagaagcta ggcctaaac cagtccgct cgcaggcta 
        gtcggactt tgctcattt ttcttcgat acaatttga 
        aagaagttc aaagaaaat ggatccgtg acaatttag 
        taacgcact tgtgttatg cgaaatctt gttacacca 
        gacatgttt tcatcctgg cctgtgttc atgccgctg 
        aacttttca gtaacaatt atttcaaga agaattttt 
        ttgaaccgt tattatcat tagctgatg atccgattt 
        caaatatac gtttgtgtg tcattggtt ttttaccaa 
        tgttgtata aaatgctag tgatgcctg aagatagaa 
        agcttcaaa cgaacatag ataatattt tttcgaagt 
        tggacatga ccgagaagg ataaagatg ttaaagata 
        ttttgaaga ctaagctta aggaaatta gaaatattg 
        gaccttcga acaaacatg attatttgg tggaacagc 
        ggcggaaag aagaagaag agaataaaa tcctgcaag 
        ggaaaatgg tcgtttcca cggcggctg taggaaata 
        atgtaacag cagcgccaa aaacagcac cttcgatcg 
        catccagag atgttccat ctactacaa ccactagat 
        ctcaaccta tcacttcaa ggcaatctg tgtcatcac 
        ctcgtgtta gtaaatcac cgtcaacta tctctaaat 
        caccttcgg caggttcgg gttcaaata cggctgcaa 
        gcgatatga gcttcttag aacagcatt tttacattg 
        atgctggcg taagcttac cgcgtatcg aacaaccta 
        tagatatgg aaatacgca aagttgtga cagatttcg 
        atcaacaat tgaatttat attcggaag atccgaacg 
        taataccga ctgtctctg tcgaaaatg ttaacattg 
        aagcaatgt ctgaaagtg aattgaaaa aactggaaa 
        catcaacca ccaatatta cagacgatg ttaaagtga 
        acttgagaa agagaagta agaaaagta caaatcgca 
        aatcacttc taagtccac caaaagata tctgcttga 
        aatacaaaa gatattcga gtgtgttcc cagccgatt 
        cctcaacat ctaaaacaa cattgaata gaagaagcc 
        tgaatatag gtgctggtg atttaagta aaattccag 
        tttgtgtga aaaaatcac acagtaatg ctgatatag 
        agcatgttt ttgaatgtg gagatggcg ataaagcga 
        atagaacct cgggaaagg tgaaacata aaccgaatg 
        aagccactc aaagtaaaa tcaggccta aatctgaaa 
        ttttaacac gtcttgaca aacaaaagg acgatagtg 
        caataactt ccagtatta gtaaaaata atgtgccaa 
        taagtagta ttaacaaag gaaaaacat caggattgc 
        cagtgttga aaaagaaaa cacagtccg agatcaaga 
        gcaaataat ttattttgt tacactttg tcagaaagt 
        cta
      • >TC002629 RB
        ggtcaaacc taaacaaat taacaaaaa tggatgaag 
        aactcacca atctgcagc tggacaaac caggggctt 
        ggggaaaca aaacgaacg aacaactcg caaaacaca 
        caattgact cgcagtttg aatcaaaac cgattgaca 
        gaagtcttc atttgctgc ggtaggtat ttcagattt 
        ttgaacttg ccgccaaaa aatgatgtt tgtgaaatg 
        tgtcacata acctaattt tttaagtat ttttctatt 
        tttctttct caaattaca aaaatatca caaactttt 
        aaaatgtag cttaatgtt caaccccat actttaaat 
        tacaatttt attcggtgc gttatggat ttggggtta 
        tacgattat cccccgcag acttcagtg gacgatgat 
        tattttttg ttacaagag ttgctcatg aaaggcgat 
        gacattcag aaattaagc gtaattcag gctttgccc 
        caaatcttg caatatgat ccaaacgga acacttaca 
        agacttgtg ccaaaaata caacaagaa ctgccaaaa 
        tcctcatgc gagtttcac atagccact agtaagata 
        tttaaattg ttgattgaa aagcaactg cctttgaat 
        ttaacgggg gctgtaata caaggaacc gagtctaaa 
        gaccctgtg gtttcgaat gcgtggact gagactctt 
        ttggctgtt attccaact ctgccaatt gatcaaatt 
        aaatcagca gtgataccg ttagcattg gctaaatcg 
        cagccctca agtcctgtg tattgtcgc gtttctagc 
        tgtaaaatc ttgggaaaa tgtgcgtca catccaaaa 
        atgcagtcg atagacgtc aaagcttcg attttgccg 
        gctgttcaa agtttgtgc caagattgt tttcaagac 
        gtgagggcc acaatgtgt tcgcagttg cacttcatt 
        gcacaggtg tacgcaaat aaaatgaaa ataattcta 
        atttttcga caggacgtc ttatgcaga gaacaatgc 
        gctttcaat ttacccgca gtcacacaa ttcacagtg 
        gctcgtata ccaaaccag ttggagctc tggcatacc 
        atcttcagt ggtctagca aacgattcc tgttacatc 
        gttaggaaa accacagct gcttgtatg cacgatata 
        gccaaaatt ttgggtccc caatgtaaa ataattaaa 
        gacgattta attaaatta ttacgtgac gatgctgaa 
        gaagtacta caacaatta gtacctcat gtgggccca 
        actttggaa ttactttgc gccaatggt gtgctttcg 
        cgagaagcc aacactccg gcgtgtctc gaaatcgca 
        agagggttg ttaaagtgc cagaatgag ttagcaaaa 
        tcttacaac tggcggatt aaatcgaat tttctgcaa 
        cagttggaa cgcctgcct ttttgcatg acttccgac 
        ttcatccat cagcatttc agtccggtt attttgagt 
        tgtgttcta gaagctagg cctaaacca gtccgctcg 
        caggctagt cggactttg ctcattttt cttcgatac 
        aatttgaaa gaagttcaa agaaaatgg atccgtgac 
        aatttagta acgcacttg tgttatgcg aaatcttgt 
        tacaccaga catgttttc atcctggcc tgtgttcat 
        gccgctgaa cttttcagt aacaattat ttcaagaag 
        aattttttt gaaccgtta ttatcatta gctgatgat 
        ccgatttca aatatacgt ttgtgtgtc attggtttt 
        ttaccaatg ttgtataaa atgctagtg atgcctgaa 
        gatagaaag cttcaaacg aacatagat aatattttt 
        tcgaagttg gacatgacc gagaaggat aaagatgtt 
        aaagatatt ttgaagact aagcttaag gaaattaga 
        aatattgga ccttcgaac aaacatgat tatttggtg 
        gaacagcgg cggaaagaa gaagaagag aataaaatc 
        ctgcaaggg aaaatggtc gtttccacg gcggctgta 
        ggaaataat gtaacagca gcgccaaaa acagcacct 
        tcgatcgca tccagagat gttccatct actacaacc 
        actagatct caacctatc acttcaagg caatctgtg 
        tcatcacct cgtgttagt aaatcaccg tcaactatc 
        tctaaatca ccttcggca ggttcgggt tcaaatacg 
        gctgcaagc gatatgagc ttcttagaa cagcatttt 
        tacattgat gctggcgta agcttaccg cgtatcgaa 
        caacctata gatatggaa atacgcaaa gttgtgaca 
        gatttcgat caacaattg aatttatat tcggaagat 
        ccgaacgta ataccgact gtctctgtc gaaaatgtt 
        aacattgaa gcaatgtct gaaagtgaa ttgaaaaaa 
        ctggaaaca tcaaccacc aatattaca gacgatgtt 
        aaagtgaac ttgagaaag agaagtaag aaaagtaca 
        aatcgcaaa tcacttcta agtccacca aaagatatc 
        tgcttgaaa tacaaaaga tattcgagt gtgttccca 
        gccgattcc tcaacatct aaaacaaca ttgaataga 
        agaagcctg aatataggt gctggtgat ttaagtaaa 
        attccagtt tgtgtgaaa aaatcacac agtaatgct 
        gatatagag catgttttt gaatgtgga gatggcgat 
        aaagcgaat agaacctcg ggaaaggtg aaacataaa 
        ccgaatgaa gccactcaa agtaaaatc aggcctaaa 
        tctgaaatt ttaacacgt cttgacaaa caaaaggac 
        gatagtgca ataacttcc agtattagt aaaaataat 
        gtgccaata agtagtatt aacaaagga aaaacatca 
        ggattgcca gtgttgaaa aagaaaaca cagtccgag 
        atcaagagc aaataattt attttgtta cactttgtc 
        agaaagtct a