| General information | |||
| TC002629 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 20952529..20969283 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC002629 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00417 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC002629 RA
MDEELTNLQ LDKPGAWGN KTNEQLAKH TIDSQFESK PIDRSLHLL RKGDDIQKL SVIQALPQI LQYDPNGTL TRLVPKIQQ ELPKSSCEF HIATSKIFK LLIEKQLPL NLTGAVIQG TESKDPVVS NAWTETLLA VIPTLPIDQ IKSAVIPLA LAKSQPSSP VYCRVSSCK ILGKCASHP KMQSIDVKA SILPAVQSL CQDCFQDVR ATMCSQLHF IAQVYANKM KIILIFRQD VLCREQCAF NLPAVTQFT VARIPNQLE LWHTIFSGL ANDSCYIVR KTTAACMHD IAKILGPQC KIIKDDLIK LLRDDAEEV LQQLVPHVG PTLELLCAN GVLSREANT PACLEIARG LLKCQNELA KSYNWRIKS NFLQQLERL PFCMTSDFI HQHFSPVIL SCVLEARPK PVRSQASRT LLIFLRYNL KEVQRKWIR DNLVTHLCY AKSCYTRHV FILACVHAA ELFSNNYFK KNFFEPLLS LADDPISNI RLCVIGFLP MLYKMLVMP EDRKLQTNI DNIFSKLDM TEKDKDVKD ILKTKLKEI RNIGPSNKH DYLVEQRRK EEEENKILQ GKMVVSTAA VGNNVTAAP KTAPSIASR DVPSTTTTR SQPITSRQS VSSPRVSKS PSTISKSPS AGSGSNTAA SDMSFLEQH FYIDAGVSL PRIEQPIDM EIRKVVTDF DQQLNLYSE DPNVIPTVS VENVNIEAM SESELKKLE TSTTNITDD VKVNLRKRS KKSTNRKSL LSPPKDICL KYKRYSSVF PADSSTSKT TLNRRSLNI GAGDLSKIP VCVKKSHSN ADIEHVFEC GDGDKANRT SGKVKHKPN EATQSKIRP KSEILTRLD KQKDDSAIT SSISKNNVP ISSINKGKT SGLPVLKKK TQSEIKSK
- >TC002629 RB
MDLGLYDYP PQTSVDDDY FLLQELLMK GDDIQKLSV IQALPQILQ YDPNGTLTR LVPKIQQEL PKSSCEFHI ATSKIFKLL IEKQLPLNL TGAVIQGTE SKDPVVSNA WTETLLAVI PTLPIDQIK SAVIPLALA KSQPSSPVY CRVSSCKIL GKCASHPKM QSIDVKASI LPAVQSLCQ DCFQDVRAT MCSQLHFIA QVYANKMKI ILIFRQDVL CREQCAFNL PAVTQFTVA RIPNQLELW HTIFSGLAN DSCYIVRKT TAACMHDIA KILGPQCKI IKDDLIKLL RDDAEEVLQ QLVPHVGPT LELLCANGV LSREANTPA CLEIARGLL KCQNELAKS YNWRIKSNF LQQLERLPF CMTSDFIHQ HFSPVILSC VLEARPKPV RSQASRTLL IFLRYNLKE VQRKWIRDN LVTHLCYAK SCYTRHVFI LACVHAAEL FSNNYFKKN FFEPLLSLA DDPISNIRL CVIGFLPML YKMLVMPED RKLQTNIDN IFSKLDMTE KDKDVKDIL KTKLKEIRN IGPSNKHDY LVEQRRKEE EENKILQGK MVVSTAAVG NNVTAAPKT APSIASRDV PSTTTTRSQ PITSRQSVS SPRVSKSPS TISKSPSAG SGSNTAASD MSFLEQHFY IDAGVSLPR IEQPIDMEI RKVVTDFDQ QLNLYSEDP NVIPTVSVE NVNIEAMSE SELKKLETS TTNITDDVK VNLRKRSKK STNRKSLLS PPKDICLKY KRYSSVFPA DSSTSKTTL NRRSLNIGA GDLSKIPVC VKKSHSNAD IEHVFECGD GDKANRTSG KVKHKPNEA TQSKIRPKS EILTRLDKQ KDDSAITSS ISKNNVPIS SINKGKTSG LPVLKKKTQ SEIKSK
- >TC002629 RA
atggatgaa gaactcacc aatctgcag ctggacaaa ccaggggct tggggaaac aaaacgaac gaacaactc gcaaaacac acaattgac tcgcagttt gaatcaaaa ccgattgac agaagtctt catttgctg cggaaaggc gatgacatt cagaaatta agcgtaatt caggctttg ccccaaatc ttgcaatat gatccaaac ggaacactt acaagactt gtgccaaaa atacaacaa gaactgcca aaatcctca tgcgagttt cacatagcc actagtaag atatttaaa ttgttgatt gaaaagcaa ctgcctttg aatttaacg ggggctgta atacaagga accgagtct aaagaccct gtggtttcg aatgcgtgg actgagact cttttggct gttattcca actctgcca attgatcaa attaaatca gcagtgata ccgttagca ttggctaaa tcgcagccc tcaagtcct gtgtattgt cgcgtttct agctgtaaa atcttggga aaatgtgcg tcacatcca aaaatgcag tcgatagac gtcaaagct tcgattttg ccggctgtt caaagtttg tgccaagat tgttttcaa gacgtgagg gccacaatg tgttcgcag ttgcacttc attgcacag gtgtacgca aataaaatg aaaataatt ctaattttt cgacaggac gtcttatgc agagaacaa tgcgctttc aatttaccc gcagtcaca caattcaca gtggctcgt ataccaaac cagttggag ctctggcat accatcttc agtggtcta gcaaacgat tcctgttac atcgttagg aaaaccaca gctgcttgt atgcacgat atagccaaa attttgggt ccccaatgt aaaataatt aaagacgat ttaattaaa ttattacgt gacgatgct gaagaagta ctacaacaa ttagtacct catgtgggc ccaactttg gaattactt tgcgccaat ggtgtgctt tcgcgagaa gccaacact ccggcgtgt ctcgaaatc gcaagaggg ttgttaaag tgccagaat gagttagca aaatcttac aactggcgg attaaatcg aattttctg caacagttg gaacgcctg cctttttgc atgacttcc gacttcatc catcagcat ttcagtccg gttattttg agttgtgtt ctagaagct aggcctaaa ccagtccgc tcgcaggct agtcggact ttgctcatt tttcttcga tacaatttg aaagaagtt caaagaaaa tggatccgt gacaattta gtaacgcac ttgtgttat gcgaaatct tgttacacc agacatgtt ttcatcctg gcctgtgtt catgccgct gaacttttc agtaacaat tatttcaag aagaatttt tttgaaccg ttattatca ttagctgat gatccgatt tcaaatata cgtttgtgt gtcattggt tttttacca atgttgtat aaaatgcta gtgatgcct gaagataga aagcttcaa acgaacata gataatatt ttttcgaag ttggacatg accgagaag gataaagat gttaaagat attttgaag actaagctt aaggaaatt agaaatatt ggaccttcg aacaaacat gattatttg gtggaacag cggcggaaa gaagaagaa gagaataaa atcctgcaa gggaaaatg gtcgtttcc acggcggct gtaggaaat aatgtaaca gcagcgcca aaaacagca ccttcgatc gcatccaga gatgttcca tctactaca accactaga tctcaacct atcacttca aggcaatct gtgtcatca cctcgtgtt agtaaatca ccgtcaact atctctaaa tcaccttcg gcaggttcg ggttcaaat acggctgca agcgatatg agcttctta gaacagcat ttttacatt gatgctggc gtaagctta ccgcgtatc gaacaacct atagatatg gaaatacgc aaagttgtg acagatttc gatcaacaa ttgaattta tattcggaa gatccgaac gtaataccg actgtctct gtcgaaaat gttaacatt gaagcaatg tctgaaagt gaattgaaa aaactggaa acatcaacc accaatatt acagacgat gttaaagtg aacttgaga aagagaagt aagaaaagt acaaatcgc aaatcactt ctaagtcca ccaaaagat atctgcttg aaatacaaa agatattcg agtgtgttc ccagccgat tcctcaaca tctaaaaca acattgaat agaagaagc ctgaatata ggtgctggt gatttaagt aaaattcca gtttgtgtg aaaaaatca cacagtaat gctgatata gagcatgtt tttgaatgt ggagatggc gataaagcg aatagaacc tcgggaaag gtgaaacat aaaccgaat gaagccact caaagtaaa atcaggcct aaatctgaa attttaaca cgtcttgac aaacaaaag gacgatagt gcaataact tccagtatt agtaaaaat aatgtgcca ataagtagt attaacaaa ggaaaaaca tcaggattg ccagtgttg aaaaagaaa acacagtcc gagatcaag agcaaataa
- >TC002629 RB
atggatttg gggttatac gattatccc ccgcagact tcagtggac gatgattat tttttgtta caagagttg ctcatgaaa ggcgatgac attcagaaa ttaagcgta attcaggct ttgccccaa atcttgcaa tatgatcca aacggaaca cttacaaga cttgtgcca aaaatacaa caagaactg ccaaaatcc tcatgcgag tttcacata gccactagt aagatattt aaattgttg attgaaaag caactgcct ttgaattta acgggggct gtaatacaa ggaaccgag tctaaagac cctgtggtt tcgaatgcg tggactgag actcttttg gctgttatt ccaactctg ccaattgat caaattaaa tcagcagtg ataccgtta gcattggct aaatcgcag ccctcaagt cctgtgtat tgtcgcgtt tctagctgt aaaatcttg ggaaaatgt gcgtcacat ccaaaaatg cagtcgata gacgtcaaa gcttcgatt ttgccggct gttcaaagt ttgtgccaa gattgtttt caagacgtg agggccaca atgtgttcg cagttgcac ttcattgca caggtgtac gcaaataaa atgaaaata attctaatt tttcgacag gacgtctta tgcagagaa caatgcgct ttcaattta cccgcagtc acacaattc acagtggct cgtatacca aaccagttg gagctctgg cataccatc ttcagtggt ctagcaaac gattcctgt tacatcgtt aggaaaacc acagctgct tgtatgcac gatatagcc aaaattttg ggtccccaa tgtaaaata attaaagac gatttaatt aaattatta cgtgacgat gctgaagaa gtactacaa caattagta cctcatgtg ggcccaact ttggaatta ctttgcgcc aatggtgtg ctttcgcga gaagccaac actccggcg tgtctcgaa atcgcaaga gggttgtta aagtgccag aatgagtta gcaaaatct tacaactgg cggattaaa tcgaatttt ctgcaacag ttggaacgc ctgcctttt tgcatgact tccgacttc atccatcag catttcagt ccggttatt ttgagttgt gttctagaa gctaggcct aaaccagtc cgctcgcag gctagtcgg actttgctc atttttctt cgatacaat ttgaaagaa gttcaaaga aaatggatc cgtgacaat ttagtaacg cacttgtgt tatgcgaaa tcttgttac accagacat gttttcatc ctggcctgt gttcatgcc gctgaactt ttcagtaac aattatttc aagaagaat ttttttgaa ccgttatta tcattagct gatgatccg atttcaaat atacgtttg tgtgtcatt ggtttttta ccaatgttg tataaaatg ctagtgatg cctgaagat agaaagctt caaacgaac atagataat attttttcg aagttggac atgaccgag aaggataaa gatgttaaa gatattttg aagactaag cttaaggaa attagaaat attggacct tcgaacaaa catgattat ttggtggaa cagcggcgg aaagaagaa gaagagaat aaaatcctg caagggaaa atggtcgtt tccacggcg gctgtagga aataatgta acagcagcg ccaaaaaca gcaccttcg atcgcatcc agagatgtt ccatctact acaaccact agatctcaa cctatcact tcaaggcaa tctgtgtca tcacctcgt gttagtaaa tcaccgtca actatctct aaatcacct tcggcaggt tcgggttca aatacggct gcaagcgat atgagcttc ttagaacag catttttac attgatgct ggcgtaagc ttaccgcgt atcgaacaa cctatagat atggaaata cgcaaagtt gtgacagat ttcgatcaa caattgaat ttatattcg gaagatccg aacgtaata ccgactgtc tctgtcgaa aatgttaac attgaagca atgtctgaa agtgaattg aaaaaactg gaaacatca accaccaat attacagac gatgttaaa gtgaacttg agaaagaga agtaagaaa agtacaaat cgcaaatca cttctaagt ccaccaaaa gatatctgc ttgaaatac aaaagatat tcgagtgtg ttcccagcc gattcctca acatctaaa acaacattg aatagaaga agcctgaat ataggtgct ggtgattta agtaaaatt ccagtttgt gtgaaaaaa tcacacagt aatgctgat atagagcat gtttttgaa tgtggagat ggcgataaa gcgaataga acctcggga aaggtgaaa cataaaccg aatgaagcc actcaaagt aaaatcagg cctaaatct gaaatttta acacgtctt gacaaacaa aaggacgat agtgcaata acttccagt attagtaaa aataatgtg ccaataagt agtattaac aaaggaaaa acatcagga ttgccagtg ttgaaaaag aaaacacag tccgagatc aagagcaaa taa
- >TC002629 RA
ggtcaaacc taaacaaat taacaaaaa tggatgaag aactcacca atctgcagc tggacaaac caggggctt ggggaaaca aaacgaacg aacaactcg caaaacaca caattgact cgcagtttg aatcaaaac cgattgaca gaagtcttc atttgctgc ggaaaggcg atgacattc agaaattaa gcgtaattc aggctttgc cccaaatct tgcaatatg atccaaacg gaacactta caagacttg tgccaaaaa tacaacaag aactgccaa aatcctcat gcgagtttc acatagcca ctagtaaga tatttaaat tgttgattg aaaagcaac tgcctttga atttaacgg gggctgtaa tacaaggaa ccgagtcta aagaccctg tggtttcga atgcgtgga ctgagactc ttttggctg ttattccaa ctctgccaa ttgatcaaa ttaaatcag cagtgatac cgttagcat tggctaaat cgcagccct caagtcctg tgtattgtc gcgtttcta gctgtaaaa tcttgggaa aatgtgcgt cacatccaa aaatgcagt cgatagacg tcaaagctt cgattttgc cggctgttc aaagtttgt gccaagatt gttttcaag acgtgaggg ccacaatgt gttcgcagt tgcacttca ttgcacagg tgtacgcaa ataaaatga aaataattc taatttttc gacaggacg tcttatgca gagaacaat gcgctttca atttacccg cagtcacac aattcacag tggctcgta taccaaacc agttggagc tctggcata ccatcttca gtggtctag caaacgatt cctgttaca tcgttagga aaaccacag ctgcttgta tgcacgata tagccaaaa ttttgggtc cccaatgta aaataatta aagacgatt taattaaat tattacgtg acgatgctg aagaagtac tacaacaat tagtacctc atgtgggcc caactttgg aattacttt gcgccaatg gtgtgcttt cgcgagaag ccaacactc cggcgtgtc tcgaaatcg caagagggt tgttaaagt gccagaatg agttagcaa aatcttaca actggcgga ttaaatcga attttctgc aacagttgg aacgcctgc ctttttgca tgacttccg acttcatcc atcagcatt tcagtccgg ttattttga gttgtgttc tagaagcta ggcctaaac cagtccgct cgcaggcta gtcggactt tgctcattt ttcttcgat acaatttga aagaagttc aaagaaaat ggatccgtg acaatttag taacgcact tgtgttatg cgaaatctt gttacacca gacatgttt tcatcctgg cctgtgttc atgccgctg aacttttca gtaacaatt atttcaaga agaattttt ttgaaccgt tattatcat tagctgatg atccgattt caaatatac gtttgtgtg tcattggtt ttttaccaa tgttgtata aaatgctag tgatgcctg aagatagaa agcttcaaa cgaacatag ataatattt tttcgaagt tggacatga ccgagaagg ataaagatg ttaaagata ttttgaaga ctaagctta aggaaatta gaaatattg gaccttcga acaaacatg attatttgg tggaacagc ggcggaaag aagaagaag agaataaaa tcctgcaag ggaaaatgg tcgtttcca cggcggctg taggaaata atgtaacag cagcgccaa aaacagcac cttcgatcg catccagag atgttccat ctactacaa ccactagat ctcaaccta tcacttcaa ggcaatctg tgtcatcac ctcgtgtta gtaaatcac cgtcaacta tctctaaat caccttcgg caggttcgg gttcaaata cggctgcaa gcgatatga gcttcttag aacagcatt tttacattg atgctggcg taagcttac cgcgtatcg aacaaccta tagatatgg aaatacgca aagttgtga cagatttcg atcaacaat tgaatttat attcggaag atccgaacg taataccga ctgtctctg tcgaaaatg ttaacattg aagcaatgt ctgaaagtg aattgaaaa aactggaaa catcaacca ccaatatta cagacgatg ttaaagtga acttgagaa agagaagta agaaaagta caaatcgca aatcacttc taagtccac caaaagata tctgcttga aatacaaaa gatattcga gtgtgttcc cagccgatt cctcaacat ctaaaacaa cattgaata gaagaagcc tgaatatag gtgctggtg atttaagta aaattccag tttgtgtga aaaaatcac acagtaatg ctgatatag agcatgttt ttgaatgtg gagatggcg ataaagcga atagaacct cgggaaagg tgaaacata aaccgaatg aagccactc aaagtaaaa tcaggccta aatctgaaa ttttaacac gtcttgaca aacaaaagg acgatagtg caataactt ccagtatta gtaaaaata atgtgccaa taagtagta ttaacaaag gaaaaacat caggattgc cagtgttga aaaagaaaa cacagtccg agatcaaga gcaaataat ttattttgt tacactttg tcagaaagt cta
- >TC002629 RB
ggtcaaacc taaacaaat taacaaaaa tggatgaag aactcacca atctgcagc tggacaaac caggggctt ggggaaaca aaacgaacg aacaactcg caaaacaca caattgact cgcagtttg aatcaaaac cgattgaca gaagtcttc atttgctgc ggtaggtat ttcagattt ttgaacttg ccgccaaaa aatgatgtt tgtgaaatg tgtcacata acctaattt tttaagtat ttttctatt tttctttct caaattaca aaaatatca caaactttt aaaatgtag cttaatgtt caaccccat actttaaat tacaatttt attcggtgc gttatggat ttggggtta tacgattat cccccgcag acttcagtg gacgatgat tattttttg ttacaagag ttgctcatg aaaggcgat gacattcag aaattaagc gtaattcag gctttgccc caaatcttg caatatgat ccaaacgga acacttaca agacttgtg ccaaaaata caacaagaa ctgccaaaa tcctcatgc gagtttcac atagccact agtaagata tttaaattg ttgattgaa aagcaactg cctttgaat ttaacgggg gctgtaata caaggaacc gagtctaaa gaccctgtg gtttcgaat gcgtggact gagactctt ttggctgtt attccaact ctgccaatt gatcaaatt aaatcagca gtgataccg ttagcattg gctaaatcg cagccctca agtcctgtg tattgtcgc gtttctagc tgtaaaatc ttgggaaaa tgtgcgtca catccaaaa atgcagtcg atagacgtc aaagcttcg attttgccg gctgttcaa agtttgtgc caagattgt tttcaagac gtgagggcc acaatgtgt tcgcagttg cacttcatt gcacaggtg tacgcaaat aaaatgaaa ataattcta atttttcga caggacgtc ttatgcaga gaacaatgc gctttcaat ttacccgca gtcacacaa ttcacagtg gctcgtata ccaaaccag ttggagctc tggcatacc atcttcagt ggtctagca aacgattcc tgttacatc gttaggaaa accacagct gcttgtatg cacgatata gccaaaatt ttgggtccc caatgtaaa ataattaaa gacgattta attaaatta ttacgtgac gatgctgaa gaagtacta caacaatta gtacctcat gtgggccca actttggaa ttactttgc gccaatggt gtgctttcg cgagaagcc aacactccg gcgtgtctc gaaatcgca agagggttg ttaaagtgc cagaatgag ttagcaaaa tcttacaac tggcggatt aaatcgaat tttctgcaa cagttggaa cgcctgcct ttttgcatg acttccgac ttcatccat cagcatttc agtccggtt attttgagt tgtgttcta gaagctagg cctaaacca gtccgctcg caggctagt cggactttg ctcattttt cttcgatac aatttgaaa gaagttcaa agaaaatgg atccgtgac aatttagta acgcacttg tgttatgcg aaatcttgt tacaccaga catgttttc atcctggcc tgtgttcat gccgctgaa cttttcagt aacaattat ttcaagaag aattttttt gaaccgtta ttatcatta gctgatgat ccgatttca aatatacgt ttgtgtgtc attggtttt ttaccaatg ttgtataaa atgctagtg atgcctgaa gatagaaag cttcaaacg aacatagat aatattttt tcgaagttg gacatgacc gagaaggat aaagatgtt aaagatatt ttgaagact aagcttaag gaaattaga aatattgga ccttcgaac aaacatgat tatttggtg gaacagcgg cggaaagaa gaagaagag aataaaatc ctgcaaggg aaaatggtc gtttccacg gcggctgta ggaaataat gtaacagca gcgccaaaa acagcacct tcgatcgca tccagagat gttccatct actacaacc actagatct caacctatc acttcaagg caatctgtg tcatcacct cgtgttagt aaatcaccg tcaactatc tctaaatca ccttcggca ggttcgggt tcaaatacg gctgcaagc gatatgagc ttcttagaa cagcatttt tacattgat gctggcgta agcttaccg cgtatcgaa caacctata gatatggaa atacgcaaa gttgtgaca gatttcgat caacaattg aatttatat tcggaagat ccgaacgta ataccgact gtctctgtc gaaaatgtt aacattgaa gcaatgtct gaaagtgaa ttgaaaaaa ctggaaaca tcaaccacc aatattaca gacgatgtt aaagtgaac ttgagaaag agaagtaag aaaagtaca aatcgcaaa tcacttcta agtccacca aaagatatc tgcttgaaa tacaaaaga tattcgagt gtgttccca gccgattcc tcaacatct aaaacaaca ttgaataga agaagcctg aatataggt gctggtgat ttaagtaaa attccagtt tgtgtgaaa aaatcacac agtaatgct gatatagag catgttttt gaatgtgga gatggcgat aaagcgaat agaacctcg ggaaaggtg aaacataaa ccgaatgaa gccactcaa agtaaaatc aggcctaaa tctgaaatt ttaacacgt cttgacaaa caaaaggac gatagtgca ataacttcc agtattagt aaaaataat gtgccaata agtagtatt aacaaagga aaaacatca ggattgcca gtgttgaaa aagaaaaca cagtccgag atcaagagc aaataattt attttgtta cactttgtc agaaagtct a
