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Gene information
General information
TC002601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22215106..22235425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002601
    iBeetle screen iB_00411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002601 RA
      MPNTTLENS PNAVLNKSV AERYMHLPC PENKVKATY 
      IWIDGTGEN VRAKTRTVD FVPKKPEEL PVWNYDGSS 
      TYQAEGTNS DTYLCPVAI YNDPFNGGN HKLVMCDTY 
      KYNKKPTET NHRHTCLQA MEAAKDTKP WFGIEQEYT 
      LLDMDMRPL GWPKNGFPG PQGPYYCGV GANKVYARD 
      IVESHYSAC LYAGINIAG TNAEVMPAQ WEYQVGPCE 
      GISIGDELW VSRYILHRV AEDFGVIVT FDPKPIEGD 
      WNGAGAHTN FSTKAMRED GGIIEIEKA IDKLSRNHL 
      RHIQAYDPK GGKDNERRL TGRHETSSI HDFSAGVAN 
      RGASIRIPR GCAEDKKGY LEDRRPSSN CDPYAVCEA 
      LVRTCILDE
    CDS sequence
    • >TC002601 RA
      atgccgaat acaaccctt gagaactca cccaatgcg 
      gtgctcaac aagtcagtg gctgaaagg tacatgcat 
      ttaccgtgc cccgaaaat aaggtaaaa gccacctac 
      atatggatt gatgggact ggtgaaaat gtcagggcg 
      aaaacacgc acagtcgat ttcgtccca aagaaaccc 
      gaagagttg ccggtttgg aactacgac ggcagttcc 
      acataccaa gccgaaggc accaactct gacacatat 
      ctatgtcct gtcgccatc tacaacgac cccttcaat 
      gggggcaac cacaagttg gtcatgtgc gacacctac 
      aaatacaac aaaaaacca accgaaact aaccaccgg 
      cacacgtgt ctgcaagcc atggaggcg gctaaagac 
      accaaacct tggttcggt atcgagcag gagtacact 
      ttgctcgac atggatatg agacctttg gggtggccc 
      aagaatggg tttccggga ccccaaggg ccttactat 
      tgcggtgtg ggggctaat aaggtctac gcacgtgat 
      attgtagag tctcactac agtgcttgt ctgtatgct 
      ggtattaat attgctgga accaatgct gaggtcatg 
      ccagctcaa tgggagtat caggttggt ccctgtgag 
      ggtatttcc atcggggat gaattgtgg gtctcgagg 
      tatattttg catcgtgtc gccgaagat tttggggtt 
      attgtcacg tttgatcct aagccgatt gaaggcgat 
      tggaatgga gcgggggct catactaac ttctccact 
      aaggcgatg agagaagat ggaggaatt attgaaatc 
      gagaaagcc atcgataag ctcagccga aaccacctt 
      agacacatc caggcctac gatcctaag ggaggaaag 
      gacaacgaa cgtcgtctc accggtcgc catgaaact 
      tcctcgatc catgacttc agtgcaggt gtggctaac 
      cgtggcgcg agtattcgt attcctcgc ggatgtgca 
      gaagataag aagggatat cttgaagat aggagacca 
      tcttcaaat tgcgatccg tacgcagta tgtgaagct 
      ttggtccga acatgtatt ctagatgaa taa
    mRNA sequence
    • >TC002601 RA
      aaggattgc acttagtca ggataaagg atatggaca 
      cgttaaatg tccataatg aataaaata tctcccctt 
      gcgcatgtg tcgtgaagg aggggaacc aagtgcata 
      aagggagtc atcgacaag tgcgtactc acagtggtt 
      ccaaatccg ctactttca catggtgta ttaaacgtt 
      tatttataa acaaaacta gacactcag ctgatcaca 
      agacgatgc cgaatacaa cccttgaga actcaccca 
      atgcggtgc tcaacaagt cagtggctg aaaggtaca 
      tgcatttac cgtgccccg aaaataagg taaaagcca 
      cctacatat ggattgatg ggactggtg aaaatgtca 
      gggcgaaaa cacgcacag tcgatttcg tcccaaaga 
      aacccgaag agttgccgg tttggaact acgacggca 
      gttccacat accaagccg aaggcacca actctgaca 
      catatctat gtcctgtcg ccatctaca acgacccct 
      tcaatgggg gcaaccaca agttggtca tgtgcgaca 
      cctacaaat acaacaaaa aaccaaccg aaactaacc 
      accggcaca cgtgtctgc aagccatgg aggcggcta 
      aagacacca aaccttggt tcggtatcg agcaggagt 
      acactttgc tcgacatgg atatgagac ctttggggt 
      ggcccaaga atgggtttc cgggacccc aagggcctt 
      actattgcg gtgtggggg ctaataagg tctacgcac 
      gtgatattg tagagtctc actacagtg cttgtctgt 
      atgctggta ttaatattg ctggaacca atgctgagg 
      tcatgccag ctcaatggg agtatcagg ttggtccct 
      gtgagggta tttccatcg gggatgaat tgtgggtct 
      cgaggtata ttttgcatc gtgtcgccg aagattttg 
      gggttattg tcacgtttg atcctaagc cgattgaag 
      gcgattgga atggagcgg gggctcata ctaacttct 
      ccactaagg cgatgagag aagatggag gaattattg 
      aaatcgaga aagccatcg ataagctca gccgaaacc 
      accttagac acatccagg cctacgatc ctaagggag 
      gaaaggaca acgaacgtc gtctcaccg gtcgccatg 
      aaacttcct cgatccatg acttcagtg caggtgtgg 
      ctaaccgtg gcgcgagta ttcgtattc ctcgcggat 
      gtgcagaag ataagaagg gatatcttg aagatagga 
      gaccatctt caaattgcg atccgtacg cagtatgtg 
      aagctttgg tccgaacat gtattctag atgaataag 
      aaacacgtt cagtcgtgt tttttaaac caattttat 
      tatttttgt ttatttctc tttcttctg tataatttg 
      tataatacg acttatatg ttcgtttgg agaagtttt 
      attcttcaa tgtgacaga aactcactg tattcagta 
      tatgtaaat atataacaa tgtcatatc agtaggttt 
      aagagctaa tatgcatgc aacgaaata ttggttgat 
      gggaatagc acatgctct attaaaatg tattttgtt 
      acgttgtga aatagttta tacaagtta ttgtaattc 
      caacaattc aaatatatt tgctatgtt cac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY