Status
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Gene information
General information
TC032572
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002594,TC002593
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22385361..22484403
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032572
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00408

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    iBeetle screen iB_07047

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032572 RA
      MATTVAMNE SNHVVYNRT ESTESGWEN 
      PFRPGGDLS READEIVEL IKGGKPITP 
      TPDSSAPPL PSLDETDTQ KNQTANNLS 
      EPITQSPKK ASELNATPK SANGNVKND 
      KPNAPGAVD VKRGTIKPD GDVEHVTIK 
      KKPKCKCCV IQ
    • >TC032572 RB
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
      SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
      PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
      EEPEPSPIP ENANPFPTD QDQTETERE KVRVTFFQQ 
      SKPQEVDLP DEPVVFTDA DFIMATTVA MNESNHVVY 
      NRTESTESG WENPFRPGG DLSREADEI VELIKGGKP 
      ITPTPDSSA PPLPSLDET DTQKNQTAN NLSEPITQS 
      PKKASELNA TPKSANGNV KNDKPNAPG AVDVKRGTI 
      KPDGDVEHV TIKKKPKCK CCVIQ
    • >TC032572 RC
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
      SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
      PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
      EEPEPSPIP ENANPFPTD QDQTEASSK FGNWWFAST 
      WRRFSKVSQ CKCFCGRAS SAYQSQSIG VDKEPNEKK 
      CE
    • >TC032572 RD
      MLSYMLPVD EKPPPAPNR GHMQLPLAS ETLTNGRTS 
      SPISKETSV SSFVSDVTT ATASHSSMT PTSTSDKGK 
      PKLVINGGK HQTLKRVSF GSSKGSMVE TLIFESPVQ 
      EEPEPSPIP ENANPFPTD QDQTE
    CDS sequence
    • >TC032572 RA
      atggccacc acggtagct atgaatgaa tcgaatcat 
      gtcgtctac aataggacg gaaagtacg gaaagcggc 
      tgggagaac cccttccga cctggcggc gacttgagt 
      cgagaggcc gacgagata gtagaatta ataaaaggg 
      ggcaaaccg ataacgcct acacccgac agttcggcg 
      cccccttta ccctcctta gacgaaacc gatacgcaa 
      aagaaccaa acagccaac aatctatcg gaacccata 
      acacaaagc ccgaagaaa gccagcgaa ctgaatgcc 
      accccgaaa agtgctaac ggcaacgtc aaaaacgac 
      aaacccaac gctccagga gccgtcgac gttaaacga 
      gggacgatt aaaccagac ggagacgtc gaacacgtt 
      acgataaaa aagaaaccc aagtgcaag tgctgtgtt 
      atacaataa
    • >TC032572 RB
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
      ccaccggct cctaacaga ggacacatg cagttacct 
      ttggcttct gaaactcta acaaacggt agaacttca 
      agtcctatc agtaaagaa acaagtgtt agcagtttt 
      gttagcgat gtgacaaca gcaacagct agtcacagc 
      tctatgacg cctacaagc accagtgat aaaggaaag 
      cctaagtta gttatcaat ggaggaaag caccaaaca 
      ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
      atggttgaa acgcttatt tttgaaagt cctgtccaa 
      gaagaaccc gagccgagt ccgattcct gaaaatgca 
      aaccctttt cctacagat caagatcaa actgaaacc 
      gaacgagaa aaagtgcga gtgacgttc tttcaacaa 
      tcaaaacct caagaagtc gatttaccg gatgaacct 
      gtcgttttt accgacgct gatttcata atggccacc 
      acggtagct atgaatgaa tcgaatcat gtcgtctac 
      aataggacg gaaagtacg gaaagcggc tgggagaac 
      cccttccga cctggcggc gacttgagt cgagaggcc 
      gacgagata gtagaatta ataaaaggg ggcaaaccg 
      ataacgcct acacccgac agttcggcg cccccttta 
      ccctcctta gacgaaacc gatacgcaa aagaaccaa 
      acagccaac aatctatcg gaacccata acacaaagc 
      ccgaagaaa gccagcgaa ctgaatgcc accccgaaa 
      agtgctaac ggcaacgtc aaaaacgac aaacccaac 
      gctccagga gccgtcgac gttaaacga gggacgatt 
      aaaccagac ggagacgtc gaacacgtt acgataaaa 
      aagaaaccc aagtgcaag tgctgtgtt atacaataa
    • >TC032572 RC
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
      ccaccggct cctaacaga ggacacatg cagttacct 
      ttggcttct gaaactcta acaaacggt agaacttca 
      agtcctatc agtaaagaa acaagtgtt agcagtttt 
      gttagcgat gtgacaaca gcaacagct agtcacagc 
      tctatgacg cctacaagc accagtgat aaaggaaag 
      cctaagtta gttatcaat ggaggaaag caccaaaca 
      ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
      atggttgaa acgcttatt tttgaaagt cctgtccaa 
      gaagaaccc gagccgagt ccgattcct gaaaatgca 
      aaccctttt cctacagat caagatcaa actgaagca 
      tcatcgaag ttcggaaat tggtggttt gcgagtact 
      tggcggcgg ttttccaaa gtcagtcag tgcaagtgc 
      ttttgtggt cgcgcgtcc tctgcctat caaagccaa 
      agcatcggc gtcgataaa gaaccgaac gagaaaaag 
      tgcgagtga
    • >TC032572 RD
      atgttgtcg tacatgcta ccagtggac gaaaaaccg 
      ccaccggct cctaacaga ggacacatg cagttacct 
      ttggcttct gaaactcta acaaacggt agaacttca 
      agtcctatc agtaaagaa acaagtgtt agcagtttt 
      gttagcgat gtgacaaca gcaacagct agtcacagc 
      tctatgacg cctacaagc accagtgat aaaggaaag 
      cctaagtta gttatcaat ggaggaaag caccaaaca 
      ctgaaaagg gtatcgttt ggtagctcc aaggggtcc 
      atggttgaa acgcttatt tttgaaagt cctgtccaa 
      gaagaaccc gagccgagt ccgattcct gaaaatgca 
      aaccctttt cctacagat caagatcaa actgagtaa
    mRNA sequence
    • >TC032572 RA
      cacccactt gtcggattg tatggtggc gctctgttg 
      caaaacagc gaactatcg ccaatccgc gatgtttga 
      ggcgtggcg ttcgaagcg tgttgcgta cgagtgtta 
      aatatttcg atgtaataa ttttataac ctcgtagag 
      catcatcga agttcggaa attggtggt ttgcgagta 
      cttggcggc ggttttcca aagtcagtc agtgcaagt 
      gcttttgtg gtcgcgcgt cctctgcct atcaaagcc 
      aaagcatcg gcgtcgata aagaaccga acgagaaaa 
      agtgcgagt gacgttctt tcaacaatc aaaacctca 
      agaagtcga tttaccgga tgaacctgt cgtttttac 
      cgacgctga tttcataat ggccaccac ggtagctat 
      gaatgaatc gaatcatgt cgtctacaa taggacgga 
      aagtacgga aagcggctg ggagaaccc cttccgacc 
      tggcggcga cttgagtcg agaggccga cgagatagt 
      agaattaat aaaaggggg caaaccgat aacgcctac 
      acccgacag ttcggcgcc ccctttacc ctccttaga 
      cgaaaccga tacgcaaaa gaaccaaac agccaacaa 
      tctatcgga acccataac acaaagccc gaagaaagc 
      cagcgaact gaatgccac cccgaaaag tgctaacgg 
      caacgtcaa aaacgacaa acccaacgc tccaggagc 
      cgtcgacgt taaacgagg gacgattaa accagacgg 
      agacgtcga acacgttac gataaaaaa gaaacccaa 
      gtgcaagtg ctgtgttat acaataact gctgacaga 
      tagtcaata gacttgtta ctgttaaat attttataa 
      aaaataata gcaaggtgt gagtaaaaa cgattcggt 
      tttgcgaga tggtggtgc taactgtga tgtttttag 
      tacaaaaaa agcatctga tggccgatc gaccaattc 
      gataaaatc ttccacaca aagcagttt tcaacctaa 
      cgaaagtaa aaaattgca aacaaaaca tatcaatcc 
      agtaataat tttgttgca attaaaaca gtcgatcag 
      tggatcaga taatcgccc agaagtaca cgaaataat 
      caaaaactg caaccgatt tcaggattt tcgttgaga 
      agaaacgaa accgaaggt ttttacgcc acccagagc 
      tgagaaatg cttacctat gctttaacc agtcacaca 
      aatactagt ggtgcatgt tcaatctct acttaattt 
      tcaagcaat actgaatat ttagttgta tggccaata 
      ggaattgtc cgccttgtc cgtgacaga aagttttaa 
      attcgactc tagtcaaaa ttatttttg tgattgtaa 
      acggacaag gtggaacag tgtggttat tggcgctgt 
      tattctact aaacgaatg tgattatat acagttata 
      aaatttacc atattttta cattgtatt agagttgtt 
      tgtgtttcg cgcttattt cttcacggt tgtgggagt 
      tttctacag atgagataa acaaacacg gaatatggg 
      aacacaaac agcggttta tatttagtt taacatttg 
      tataaatat acaatgagt ctagaatgg atgatagta 
      caagtattt tttatatat tttagcctt tcatcaaca 
      tttttacta ctgtatgac agaaatttc ggattaacg 
      gtgtattca gtagcgaga tttagttgt ctatgtttt 
      actatttaa cttgtaaat gttctataa ataggtgcg 
      acgttctag ttattgttg ttaatatta tttgttgtg 
      tttgtgtgt ctggaatga aagtcggaa acatcggca 
      ttatttttg gtaaaaaat aatacttga taaagataa 
      ttaaatcgt accggaacg atcagacgg ttggtaata 
      agtttcatt agtgaaatt tatttgnnn nnnnnnnnn 
      nnnnnnnnn nnnaacacg aattgtgca gagattatt 
      taatatttt aaatataag tacataacg tccctgttt 
      atatgtgta tgtaaagat tgcagctta taatatgct 
      taagatgta tagatttta gattgtaaa tcgatcatg 
      tttgtttga agcactcca aactctttt ttaatgata 
      ttttacatg aggaaattt ttattaata tattatttt 
      aatcttttg tgtattata ttgtaatat aaatctaat 
      ttgtatacc aagccgcat agcattaaa taatttgtg 
      atgtc
    • >TC032572 RB
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
      tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
      taaacaccg gctcctcca gctaaaatc gcgctaaga 
      ctgcatagc tcctccatc gaattcgaa cggtggtgc 
      agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
      cacttgccc cagtttcga gcccagatc ggtgcttct 
      tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
      ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
      atgctacca gtggacgaa aaaccgcca ccggctcct 
      aacagagga cacatgcag ttacctttg gcttctgaa 
      actctaaca aacggtaga acttcaagt cctatcagt 
      aaagaaaca agtgttagc agttttgtt agcgatgtg 
      acaacagca acagctagt cacagctct atgacgcct 
      acaagcacc agtgataaa ggaaagcct aagttagtt 
      atcaatgga ggaaagcac caaacactg aaaagggta 
      tcgtttggt agctccaag gggtccatg gttgaaacg 
      cttattttt gaaagtcct gtccaagaa gaacccgag 
      ccgagtccg attcctgaa aatgcaaac ccttttcct 
      acagatcaa gatcaaact gaaaccgaa cgagaaaaa 
      gtgcgagtg acgttcttt caacaatca aaacctcaa 
      gaagtcgat ttaccggat gaacctgtc gtttttacc 
      gacgctgat ttcataatg gccaccacg gtagctatg 
      aatgaatcg aatcatgtc gtctacaat aggacggaa 
      agtacggaa agcggctgg gagaacccc ttccgacct 
      ggcggcgac ttgagtcga gaggccgac gagatagta 
      gaattaata aaagggggc aaaccgata acgcctaca 
      cccgacagt tcggcgccc cctttaccc tccttagac 
      gaaaccgat acgcaaaag aaccaaaca gccaacaat 
      ctatcggaa cccataaca caaagcccg aagaaagcc 
      agcgaactg aatgccacc ccgaaaagt gctaacggc 
      aacgtcaaa aacgacaaa cccaacgct ccaggagcc 
      gtcgacgtt aaacgaggg acgattaaa ccagacgga 
      gacgtcgaa cacgttacg ataaaaaag aaacccaag 
      tgcaagtgc tgtgttata caataactg ctgacagat 
      agtcaatag acttgttac tgttaaata ttttataaa 
      aaataatag caaggtgtg agtaaaaac gattcggtt 
      ttgcgagat ggtggtgct aactgtgat gtttttagt 
      acaaaaaaa gcatctgat ggccgatcg accaattcg 
      ataaaatct tccacacaa agcagtttt caacctaac 
      gaaagtaaa aaattgcaa acaaaacat atcaatcca 
      gtaataatt ttgttgcaa ttaaaacag tcgatcagt 
      ggatcagat aatcgccca gaagtacac gaaataatc 
      aaaaactgc aaccgattt caggatttt cgttgagaa 
      gaaacgaaa ccgaaggtt tttacgcca cccagagct 
      gagaaatgc ttacctatg ctttaacca gtcacacaa 
      atactagtg gtgcatgtt caatctcta cttaatttt 
      caagcaata ctgaatatt tagttgtat ggccaatag 
      gaattgtcc gccttgtcc gtgacagaa agttttaaa 
      ttcgactct agtcaaaat tatttttgt gattgtaaa 
      cggacaagg tggaacagt gtggttatt ggcgctgtt 
      attctacta aacgaatgt gattatata cagttataa 
      aatttacca tatttttac attgtatta gagttgttt 
      gtgtttcgc gcttatttc ttcacggtt gtgggagtt 
      ttctacaga tgagataaa caaacacgg aatatggga 
      acacaaaca gcggtttat atttagttt aacatttgt 
      ataaatata caatgagtc tagaatgga tgatagtac 
      aagtatttt ttatatatt ttagccttt catcaacat 
      ttttactac tgtatgaca gaaatttcg gattaacgg 
      tgtattcag tagcgagat ttagttgtc tatgtttta 
      ctatttaac ttgtaaatg ttctataaa taggtgcga 
      cgttctagt tattgttgt taatattat ttgttgtgt 
      ttgtgtgtc tggaatgaa agtcggaaa catcggcat 
      tatttttgg taaaaaata atacttgat aaagataat 
      taaatcgta ccggaacga tcagacggt tggtaataa 
      gtttcatta gtgaaattt atttgnnnn nnnnnnnnn 
      nnnnnnnnn nnaacacga attgtgcag agattattt 
      aatatttta aatataagt acataacgt ccctgttta 
      tatgtgtat gtaaagatt gcagcttat aatatgctt 
      aagatgtat agattttag attgtaaat cgatcatgt 
      ttgtttgaa gcactccaa actcttttt taatgatat 
      tttacatga ggaaatttt tattaatat attatttta 
      atcttttgt gtattatat tgtaatata aatctaatt 
      tgtatacca agccgcata gcattaaat aatttgtga 
      tgtc
    • >TC032572 RC
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
      tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
      taaacaccg gctcctcca gctaaaatc gcgctaaga 
      ctgcatagc tcctccatc gaattcgaa cggtggtgc 
      agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
      cacttgccc cagtttcga gcccagatc ggtgcttct 
      tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
      ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
      atgctacca gtggacgaa aaaccgcca ccggctcct 
      aacagagga cacatgcag ttacctttg gcttctgaa 
      actctaaca aacggtaga acttcaagt cctatcagt 
      aaagaaaca agtgttagc agttttgtt agcgatgtg 
      acaacagca acagctagt cacagctct atgacgcct 
      acaagcacc agtgataaa ggaaagcct aagttagtt 
      atcaatgga ggaaagcac caaacactg aaaagggta 
      tcgtttggt agctccaag gggtccatg gttgaaacg 
      cttattttt gaaagtcct gtccaagaa gaacccgag 
      ccgagtccg attcctgaa aatgcaaac ccttttcct 
      acagatcaa gatcaaact gaagcatca tcgaagttc 
      ggaaattgg tggtttgcg agtacttgg cggcggttt 
      tccaaagtc agtcagtgc aagtgcttt tgtggtcgc 
      gcgtcctct gcctatcaa agccaaagc atcggcgtc 
      gataaagaa ccgaacgag aaaaagtgc gagtgacgt 
      tctttcaac aatcaaaac ctcaagaag tcgatttac 
      cggatgaac ctgtcgttt ttaccgacg ctgatttca 
      taatggcca ccacggtag ctatgaatg aatcgaatc 
      atgtcgtct acaatagga cggaaaggt gagtttttg 
      tattgctgt aagtgacta tgtcacact aaaagctca 
      caaagact
    • >TC032572 RD
      tacaaagca gaagtagag tttcgatgg tccgctgtg 
      tacaaatcg cgagctgcg ccgtgagta cgtcgcgga 
      taaacaccg gctcctcca gctaaaatc gcgctaaga 
      ctgcatagc tcctccatc gaattcgaa cggtggtgc 
      agtgtaggt gtggtgtcg tggtcaagg gtaagccga 
      cacttgccc cagtttcga gcccagatc ggtgcttct 
      tcgattgtg accgtgaaa gtgtattag tgtaaaatg 
      ttaaaatta agttagtgc ttcgtaatg ttgtcgtac 
      atgctacca gtggacgaa aaaccgcca ccggctcct 
      aacagagga cacatgcag ttacctttg gcttctgaa 
      actctaaca aacggtaga acttcaagt cctatcagt 
      aaagaaaca agtgttagc agttttgtt agcgatgtg 
      acaacagca acagctagt cacagctct atgacgcct 
      acaagcacc agtgataaa ggaaagcct aagttagtt 
      atcaatgga ggaaagcac caaacactg aaaagggta 
      tcgtttggt agctccaag gggtccatg gttgaaacg 
      cttattttt gaaagtcct gtccaagaa gaacccgag 
      ccgagtccg attcctgaa aatgcaaac ccttttcct 
      acagatcaa gatcaaact gagtaagtt atgttttaa 
      ctatttaat tactttagt ttataacgt tttttttat 
      atttgtatt ccaaaaact ctaataact ctcttcata 
      agactttaa atcttgatg gaacaatca ccatattca 
      tcgcttaag ctttccagg ttttgttag gataattta 
      aacaccaac atacaaaaa gttgtttgg acttaattt 
      acattgacc tgatgatat ttgaaagca aataaaata 
      tttaaaaat tt
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