Status
Please wait ...
Gene information
General information
TC002590 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22603214..22614624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002590
    iBeetle screen iB_00406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002590 RA
      MSDFNPFAA LIEESTDKS NDSTNTKQG HVKNISSIL 
      EEIFAFTLD PKVSEKKHF LLLEEVANT FSRNDLDLL 
      ILQHALFDR LFMCNEENL FIKTAKQNF RNTHSFESN 
      VLTYLCNCF RNLSDDMNL ESGEKAKVK SLIIQNAVT 
      ALIQSEIYE GQKLEQQLI DILKDNTDN GILFFKDVC 
      KGVLDEEEG GEAFLKDKL GQILLVLTD DIIKSNIVT 
      FDYGIFNVL NVFTTEEYL ASIFLDVKP VKFSAGSDY 
      ANTPLGAIF NVSILPKTP NAIYEHFQD PTDQASILS 
      AEGILWSNV DKLTEHTHA FVLSLLTCS PQIKNKTLE 
      WLGLCIKAN IDRGKLWSA QSPPELNLV NYTSVSDGF 
      MINFGNVML RLCRPFCNN FKDKKILKV DPTYCSVPD 
      DQCCEKGVH LPGMNTETC LIPNDSDDE GKLLTAEKY 
      NFVTECFYF AHKAVNLGY QVTVDKLVR LNHEVGRME 
      RAYLDAVAQ AAGNNDLVD SLKRRMTQQ LTKYLSLKA 
      QLSDPVLLN LLFDFVSTT TYWLCQVAV KVNFEDQRK 
      TFAPLDEIP INFPLPDAI PSTLKSIPE FLIENIVCY 
      LVFLRRFNP KIFEEQGYE KLKPILDFI LIYMGSPER 
      LRNPHVRAR LAEALESLL PRHEDEPPS FNAFGGFQR 
      EMLFTQHEH RSEIVSSLL KVFVGIEMT GQSVEFEQK 
      FNYRRPMYT VMDYLWTKE EFKTSFKML AQEAEKNVE 
      AVTPPLFLR FVNLLMNDA VYLLDEALA NMAKLKEMQ 
      TARQNGEWD SLPAQERTQ NLGYMHHIG MIAKFDNIL 
      GRDTIKTLE KLTSEITIV FTHSTMVDR VAAMLNYFL 
      YNLVGPKKK NFKVKDSKE YSFDPATTV LNICKIYVN 
      LKESSSFCL AVSQDGRSY SPQLFSYAE DVLIRIGGG 
      SLIGELKEV AMRVAEKAQ EQQASEEAI AEAPEHFLD 
      PIMSTLMTD PVILPSSKQ TVDRTTIAR HLLSDQTDP 
      FNRAPLSMD QVIPNTELA EEIRNWLDE RKKK
    CDS sequence
    • >TC002590 RA
      atgtcagac ttcaacccg ttcgctgcc ctaatagaa 
      gaatccacg gataaatcg aacgattca acaaacacg 
      aaacaagga catgttaaa aacatcagc agcatcctc 
      gaagagata ttcgcattt acattagac ccgaaagtt 
      agcgagaag aaacacttc ttgttgctg gaggaagtt 
      gcaaacact ttcagccgg aatgatctt gacctcttg 
      atcctccaa catgcccta tttgatcgc ctgtttatg 
      tgtaacgaa gagaatctg ttcataaaa actgccaaa 
      cagaacttc aggaacacg cattcgttt gaatcgaac 
      gtgctcact tatctgtgc aattgcttc aggaatttg 
      agcgatgac atgaacctt gagagtgga gaaaaggcc 
      aaagttaag tcgttgatt attcaaaac gctgtgacg 
      gccctcata cagtcggaa atctacgaa ggccaaaaa 
      ctagagcag cagttgatt gatattctt aaagataac 
      actgataac ggtatactt tttttcaaa gatgtgtgt 
      aagggagtt ctggatgag gaagaagga ggtgaggct 
      tttcttaag gataaattg gggcaaatt ttactggtg 
      ttaaccgat gacataatt aaatcaaat atcgtcact 
      tttgactat ggaattttc aatgttttg aatgttttt 
      acaactgag gaatatctc gccagtatt tttcttgat 
      gtaaaacct gtaaagttt agtgctggt tcagactat 
      gcgaacacc ccgttaggg gccattttc aacgtttca 
      atcttgccc aaaacaccg aatgcgatt tatgagcac 
      ttccaggac cctactgat caagctagc atcttgtcg 
      gctgaagga attctctgg agcaacgtc gataaacta 
      accgaacac acgcacgct tttgtccta agtttacta 
      acttgcagt ccacaaatt aaaaataaa accctggaa 
      tggctagga ctttgtatc aaagctaac attgatcgt 
      ggaaaattg tggagcgct caatcacca cctgagtta 
      aatcttgtc aattataca agtgtgtct gatggtttt 
      atgatcaat tttgggaac gtaatgtta aggttgtgc 
      cgaccgttt tgtaataat ttcaaggat aaaaaaata 
      ctgaaagtg gatccgacc tactgttct gttcctgat 
      gatcaatgt tgtgaaaag ggtgtccat ttgcctggc 
      atgaatacg gaaacatgt ctaataccg aatgattca 
      gatgatgaa ggaaagttg ctaactgct gaaaagtat 
      aattttgta acagagtgt ttttatttt gcacataaa 
      gctgttaat ttgggttat caagtcact gtggataag 
      ttggtgcgc ttaaatcac gaagtcggc cgaatggag 
      agagcctat ttggacgct gtagcacaa gcggcggga 
      aataacgat ttagttgat agtttaaaa agaagaatg 
      actcaacag ctgaccaaa tatttatca ctaaaagct 
      caattatcg gatcctgtt ttactaaat ttgctattt 
      gatttcgta tcaacgacc acttattgg ctttgccaa 
      gtcgctgtt aaagtcaat ttcgaggat caacgaaaa 
      acgtttgca cctttagac gaaattcca atcaatttt 
      ccgttacct gacgcaatt ccaagtacc ctaaaaagc 
      attccagaa tttctaatc gaaaatata gtttgttac 
      ttagtgttt ttgagacgt tttaatccg aaaattttc 
      gaagaacag ggatacgaa aaattgaaa cctatatta 
      gattttatt ttaatttat atgggatcg ccggaacgt 
      ttgagaaac ccccatgtg agagcaagg cttgctgag 
      gcattagag tctcttttg ccacgtcat gaagatgag 
      ccgccgagt tttaatgca tttggcggc ttccagcgt 
      gaaatgctt tttacccaa cacgaacat cgaagtgag 
      attgttagc agtttgctg aaagtgttt gttgggatt 
      gaaatgacg ggacaaagc gttgagttt gaacagaaa 
      ttcaattac agacggccg atgtacacc gtgatggac 
      tatttgtgg accaaggaa gaattcaaa accagtttt 
      aagatgttg gcacaggaa gccgaaaaa aacgtggaa 
      gcagtcaca cctcctcta tttttacgc tttgtcaat 
      cttctaatg aacgatgcg gtttattta ttggatgag 
      gcattggcc aatatggca aagttgaaa gaaatgcaa 
      actgcgcgt caaaacggc gaatgggat tcgttacca 
      gcccaagaa cggacccaa aacttgggc tacatgcac 
      cacatcggt atgatcgca aaattcgat aatatcttg 
      gggagagac acgattaaa actctggaa aaattgacc 
      tcggaaata acgatagta ttcacgcat tctacgatg 
      gttgatcga gtcgctgct atgctcaat tatttcttg 
      tacaattta gtcggtccc aaaaagaag aattttaag 
      gtgaaagat tcgaaagag tacagtttt gacccggca 
      acaacagtg ttgaacatt tgcaaaatt tacgttaat 
      cttaaggaa agcagttcg ttttgtttg gctgtttcg 
      caagatggg cgttcgtac agtcctcag ttgttcagt 
      tatgctgag gatgtttta attcgtatt gggggcggg 
      tctcttatt ggagaactg aaagaagtt gcaatgcgg 
      gttgcggaa aaggctcaa gaacagcaa gctagtgaa 
      gaggcgatt gccgaggct cctgaacat ttcttggat 
      cctattatg tcgactttg atgaccgat cctgtgatt 
      ctacctagt tcaaaacaa acggttgat cgtactact 
      attgccagg catctttta agcgaccaa actgatcct 
      ttcaatcgt gcacctttg tccatggat caggtgatt 
      ccgaataca gaactagct gaagaaatt cggaattgg 
      ttggatgaa aggaaaaag aaataa
    mRNA sequence
    • >TC002590 RA
      gggttttca agtgtcacg aaatgtcaa acgtggatg 
      tgatctgca attttttaa caacataaa aaatgcgtt 
      ttaaaaaat aacattcat agtaatttc gccgaattg 
      tgtgtcacg atgtcagac ttcaacccg ttcgctgcc 
      ctaatagaa gaatccacg gataaatcg aacgattca 
      acaaacacg aaacaagga catgttaaa aacatcagc 
      agcatcctc gaagagata ttcgcattt acattagac 
      ccgaaagtt agcgagaag aaacacttc ttgttgctg 
      gaggaagtt gcaaacact ttcagccgg aatgatctt 
      gacctcttg atcctccaa catgcccta tttgatcgc 
      ctgtttatg tgtaacgaa gagaatctg ttcataaaa 
      actgccaaa cagaacttc aggaacacg cattcgttt 
      gaatcgaac gtgctcact tatctgtgc aattgcttc 
      aggaatttg agcgatgac atgaacctt gagagtgga 
      gaaaaggcc aaagttaag tcgttgatt attcaaaac 
      gctgtgacg gccctcata cagtcggaa atctacgaa 
      ggccaaaaa ctagagcag cagttgatt gatattctt 
      aaagataac actgataac ggtatactt tttttcaaa 
      gatgtgtgt aagggagtt ctggatgag gaagaagga 
      ggtgaggct tttcttaag gataaattg gggcaaatt 
      ttactggtg ttaaccgat gacataatt aaatcaaat 
      atcgtcact tttgactat ggaattttc aatgttttg 
      aatgttttt acaactgag gaatatctc gccagtatt 
      tttcttgat gtaaaacct gtaaagttt agtgctggt 
      tcagactat gcgaacacc ccgttaggg gccattttc 
      aacgtttca atcttgccc aaaacaccg aatgcgatt 
      tatgagcac ttccaggac cctactgat caagctagc 
      atcttgtcg gctgaagga attctctgg agcaacgtc 
      gataaacta accgaacac acgcacgct tttgtccta 
      agtttacta acttgcagt ccacaaatt aaaaataaa 
      accctggaa tggctagga ctttgtatc aaagctaac 
      attgatcgt ggaaaattg tggagcgct caatcacca 
      cctgagtta aatcttgtc aattataca agtgtgtct 
      gatggtttt atgatcaat tttgggaac gtaatgtta 
      aggttgtgc cgaccgttt tgtaataat ttcaaggat 
      aaaaaaata ctgaaagtg gatccgacc tactgttct 
      gttcctgat gatcaatgt tgtgaaaag ggtgtccat 
      ttgcctggc atgaatacg gaaacatgt ctaataccg 
      aatgattca gatgatgaa ggaaagttg ctaactgct 
      gaaaagtat aattttgta acagagtgt ttttatttt 
      gcacataaa gctgttaat ttgggttat caagtcact 
      gtggataag ttggtgcgc ttaaatcac gaagtcggc 
      cgaatggag agagcctat ttggacgct gtagcacaa 
      gcggcggga aataacgat ttagttgat agtttaaaa 
      agaagaatg actcaacag ctgaccaaa tatttatca 
      ctaaaagct caattatcg gatcctgtt ttactaaat 
      ttgctattt gatttcgta tcaacgacc acttattgg 
      ctttgccaa gtcgctgtt aaagtcaat ttcgaggat 
      caacgaaaa acgtttgca cctttagac gaaattcca 
      atcaatttt ccgttacct gacgcaatt ccaagtacc 
      ctaaaaagc attccagaa tttctaatc gaaaatata 
      gtttgttac ttagtgttt ttgagacgt tttaatccg 
      aaaattttc gaagaacag ggatacgaa aaattgaaa 
      cctatatta gattttatt ttaatttat atgggatcg 
      ccggaacgt ttgagaaac ccccatgtg agagcaagg 
      cttgctgag gcattagag tctcttttg ccacgtcat 
      gaagatgag ccgccgagt tttaatgca tttggcggc 
      ttccagcgt gaaatgctt tttacccaa cacgaacat 
      cgaagtgag attgttagc agtttgctg aaagtgttt 
      gttgggatt gaaatgacg ggacaaagc gttgagttt 
      gaacagaaa ttcaattac agacggccg atgtacacc 
      gtgatggac tatttgtgg accaaggaa gaattcaaa 
      accagtttt aagatgttg gcacaggaa gccgaaaaa 
      aacgtggaa gcagtcaca cctcctcta tttttacgc 
      tttgtcaat cttctaatg aacgatgcg gtttattta 
      ttggatgag gcattggcc aatatggca aagttgaaa 
      gaaatgcaa actgcgcgt caaaacggc gaatgggat 
      tcgttacca gcccaagaa cggacccaa aacttgggc 
      tacatgcac cacatcggt atgatcgca aaattcgat 
      aatatcttg gggagagac acgattaaa actctggaa 
      aaattgacc tcggaaata acgatagta ttcacgcat 
      tctacgatg gttgatcga gtcgctgct atgctcaat 
      tatttcttg tacaattta gtcggtccc aaaaagaag 
      aattttaag gtgaaagat tcgaaagag tacagtttt 
      gacccggca acaacagtg ttgaacatt tgcaaaatt 
      tacgttaat cttaaggaa agcagttcg ttttgtttg 
      gctgtttcg caagatggg cgttcgtac agtcctcag 
      ttgttcagt tatgctgag gatgtttta attcgtatt 
      gggggcggg tctcttatt ggagaactg aaagaagtt 
      gcaatgcgg gttgcggaa aaggctcaa gaacagcaa 
      gctagtgaa gaggcgatt gccgaggct cctgaacat 
      ttcttggat cctattatg tcgactttg atgaccgat 
      cctgtgatt ctacctagt tcaaaacaa acggttgat 
      cgtactact attgccagg catctttta agcgaccaa 
      actgatcct ttcaatcgt gcacctttg tccatggat 
      caggtgatt ccgaataca gaactagct gaagaaatt 
      cggaattgg ttggatgaa aggaaaaag aaataagtc 
      gtttttgtt gtaaacatt ttaatgtga atgtgaaat 
      atttttatt aaaataaat tttattaaa gatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY