Status
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Gene information
General information
TC002574 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23017105..23027258
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002574
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00404

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002574 RA
      MVLADLGRK ITTALQSLS KATIINEEV LNGMLKEIC 
      AALLEADVN IRLVKKLRE NVRAVIDFD EMAGGLNKR 
      RMIQSAVFK ELVKLVDPG VKPYQPVKG KPNVIMFVG 
      LQGSGKTTT CTKLAYHYQ KKNWKSCLV CADTFRAGA 
      YDQVKQNCT KARIPFYGS YTEVDPVVI AQDGVDMFK 
      KEGFEIIIV DTSGRHKQE ESLFEEMLA VSNAIQPDN 
      IIFVMDATI GQACEAQAK AFKEKVDVG SVIITKLDG 
      HAKGGGALS AVAATNSPI IYIGTGEHI DDLEPFKTK 
      PFVSKLLGM GDIEGLIDK VNELKLEDN EELLEKIKH 
      GQFTLRDMY EQFQNIMKM GPFSQIMGM IPGFSQDFM 
      SKGSEQESM SRLKRLMTI MDSMNDDEL DSRDGAKIF 
      SKQNGRVVR VAQGSGVTE REVKELITQ YTKFAAVVK 
      KMGGIKGLF KGGDMAKNV NPAQMAKLN QQMAKMMDP 
      RVLHQMGGM PGLQNMMRQ LQAGAGGGL GNLGNLMGG 
      FGGK
    CDS sequence
    • >TC002574 RA
      atggtttta gcagactta ggtcgtaaa ataacgact 
      gctttgcag tcgttaagc aaagcaacg ataataaac 
      gaagaggtt ctcaatgga atgctcaag gaaatatgt 
      gcagctctg cttgaagcc gatgtaaat atccgtctc 
      gtgaaaaaa ctgcgggaa aatgtgagg gcggttatt 
      gattttgat gagatggca gggggcttg aacaagcgg 
      aggatgatc cagagtgcc gttttcaaa gagctcgtt 
      aaacttgtc gatcccggt gtcaagcca taccagcct 
      gttaaaggc aaacccaat gttattatg tttgttggg 
      cttcaaggc tcagggaaa accacaact tgtacaaag 
      ctggcatat cattatcag aagaaaaat tggaagtcg 
      tgtttagtg tgtgcggac acgttcagg gctggtgcc 
      tatgaccag gttaaacaa aattgcaca aaagcaaga 
      atcccgttt tacggaagt tacactgag gtggaccca 
      gtggtgata gcgcaagat ggtgtcgac atgttcaaa 
      aaagagggt tttgagatt attattgtg gatacgagc 
      ggaagacat aagcaagaa gaatcatta tttgaagag 
      atgttggca gtttcaaac gccattcaa cctgacaat 
      attattttc gtcatggac gccaccata ggtcaagct 
      tgtgaagct caagcgaaa gcttttaaa gaaaaagtc 
      gatgtaggg tctgtaatt atcacaaaa ttggacgga 
      catgccaag ggtggaggc gccctcagt gcggtggct 
      gctaccaac agtccgatt atttacatt ggtacaggg 
      gaacatatt gatgattta gagccgttc aagaccaaa 
      cctttcgtc agtaaattg ctaggaatg ggcgatatt 
      gagggtctc atagacaaa gtaaatgaa ttgaaacta 
      gaagataat gaagaattg ttagagaaa atcaaacat 
      ggacagttc acacttaga gatatgtac gaacagttt 
      cagaacatc atgaaaatg ggacccttc tcacaaatt 
      atgggaatg attccgggc tttagccaa gacttcatg 
      tcgaaaggc agcgaacaa gagtcgatg tcgcgtctc 
      aaacgcttg atgaccatc atggacagt atgaacgac 
      gacgagctc gacagcaga gacggtgca aaaattttc 
      tctaaacag aacggtcgt gtcgtacga gtagcccaa 
      ggctcaggc gttaccgaa cgtgaagtc aaagaactg 
      atcacccag tatacgaaa ttcgcagca gtcgtcaag 
      aaaatgggc ggtattaag ggccttttc aaagggggc 
      gacatggcc aaaaatgtc aatccagca caaatggcc 
      aaactcaac caacagatg gccaaaatg atggacccc 
      agagtatta caccaaatg ggtggaatg ccaggtttg 
      cagaacatg atgcgacag ttgcaagca ggcgctgga 
      ggaggatta ggtaattta gggaatttg atgggtggt 
      ttcggggga aagtaa
    mRNA sequence
    • >TC002574 RA
      ccttagcca catcccaca accagcatg tcaccaact 
      tgtcaaaat tgttgaaac atttttaac aacaaataa 
      agcattaac tgtgtgaaa cgaagcttc cacgatggt 
      tttagcaga cttaggtcg taaaataac gactgcttt 
      gcagtcgtt aagcaaagc aacgataat aaacgaaga 
      ggttctcaa tggaatgct caaggaaat atgtgcagc 
      tctgcttga agccgatgt aaatatccg tctcgtgaa 
      aaaactgcg ggaaaatgt gagggcggt tattgattt 
      tgatgagat ggcaggggg cttgaacaa gcggaggat 
      gatccagag tgccgtttt caaagagct cgttaaact 
      tgtcgatcc cggtgtcaa gccatacca gcctgttaa 
      aggcaaacc caatgttat tatgtttgt tgggcttca 
      aggctcagg gaaaaccac aacttgtac aaagctggc 
      atatcatta tcagaagaa aaattggaa gtcgtgttt 
      agtgtgtgc ggacacgtt cagggctgg tgcctatga 
      ccaggttaa acaaaattg cacaaaagc aagaatccc 
      gttttacgg aagttacac tgaggtgga cccagtggt 
      gatagcgca agatggtgt cgacatgtt caaaaaaga 
      gggttttga gattattat tgtggatac gagcggaag 
      acataagca agaagaatc attatttga agagatgtt 
      ggcagtttc aaacgccat tcaacctga caatattat 
      tttcgtcat ggacgccac cataggtca agcttgtga 
      agctcaagc gaaagcttt taaagaaaa agtcgatgt 
      agggtctgt aattatcac aaaattgga cggacatgc 
      caagggtgg aggcgccct cagtgcggt ggctgctac 
      caacagtcc gattattta cattggtac aggggaaca 
      tattgatga tttagagcc gttcaagac caaaccttt 
      cgtcagtaa attgctagg aatgggcga tattgaggg 
      tctcataga caaagtaaa tgaattgaa actagaaga 
      taatgaaga attgttaga gaaaatcaa acatggaca 
      gttcacact tagagatat gtacgaaca gtttcagaa 
      catcatgaa aatgggacc cttctcaca aattatggg 
      aatgattcc gggctttag ccaagactt catgtcgaa 
      aggcagcga acaagagtc gatgtcgcg tctcaaacg 
      cttgatgac catcatgga cagtatgaa cgacgacga 
      gctcgacag cagagacgg tgcaaaaat tttctctaa 
      acagaacgg tcgtgtcgt acgagtagc ccaaggctc 
      aggcgttac cgaacgtga agtcaaaga actgatcac 
      ccagtatac gaaattcgc agcagtcgt caagaaaat 
      gggcggtat taagggcct tttcaaagg gggcgacat 
      ggccaaaaa tgtcaatcc agcacaaat ggccaaact 
      caaccaaca gatggccaa aatgatgga ccccagagt 
      attacacca aatgggtgg aatgccagg tttgcagaa 
      catgatgcg acagttgca agcaggcgc tggaggagg 
      attaggtaa tttagggaa tttgatggg tggtttcgg 
      gggaaagta atttaggga gttgtaact atgtgaagt 
      ttttataaa aataaattt atcttttat tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY